| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
We have developed reconstitution membrane of EGF receptor (EGFR) for analyzing molecular mechanism of signal transduction across the plasma membrane. EGFR molecules were incorporated into nanodiscs with the diameter of 10-15 nm. Single-molecule FRET imaging of the transmembrane (TM)-juxtamenbrane (JM) domains of EGFR revealed that dimerization of EGFR, which is responsible for signal transduction, is induced by interactions of the JM domain with acidic membrane lipids, and inhibitory effect of the phosphorylation at the JM domain for dimerization requires acidic lipids in the membrane. Cholesterol in the membrane also induced dimerization of EGFR, however, dimer structures suggested to be different from that in membranes without cholesterol.
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