Estimation of neural activity propagation pathway in the brain by applying deep learning

• Project/Area Number: 15KT0111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Mathematical Sciences in Search of New Cooperation
• Research Institution: Keio University
• Principal Investigator: TAKATA Norio 慶應義塾大学, 医学部(信濃町), 特任講師 (50415212)
• Co-Investigator(Kenkyū-buntansha): 中江 健 京都大学, 情報学研究科, 特定研究員 (70617472)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Kenji 慶應義塾大学, 医学部, 准教授 (30329700) ; OKANO Hideyuki 慶應義塾大学, 医学部, 教授 (60160694)
• Research Collaborator: YOSHIDA Keitaro
• Project Period (FY): 2015-07-10 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 深層学習 / マウス / fMRI / 光遺伝学 / 経路推定 / 脳活動伝搬 / ディープラーニング / dropout / optogenetics

Outline of Final Research Achievements

In this study, we tried to estimate the activity propagation path in the brain and elucidate the existence of rules and features in the propagation path. Specifically, when the start site and the termination site of brain activity are clear, we aimed to clarify the nature of the propagation pathway such as whether the neural activity propagates in parallel or in series in the brain circuit. In the beginning, path estimation was performed on a small number of ROIs using fMRI measurement data (data obtained by measuring responses of the brain upon optogenetic activation of the hippocampus of an anesthetized mouse; Takata et al. 2015 PLoS One). As a result, we could estimate three paths as potential candidates.

Research Products

• [Journal Article] Optogenetic astrocyte activation evokes BOLD fMRI response with oxygen consumption without neuronal activity modulation (2018)
  • Author(s): Norio Takata, Yuki Sugiura, Keitaro Yoshida, Miwako Koizumi, Hiroshi Nishida, Kurara Honda, Ryutaro Yano, Yuji Komaki, Ko Matsui, Makoto Suematsu , Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka
  • Journal Title: Glia Volume: 印刷中 Issue: 9 Pages: 2013-2023
  • DOI: 10.1002/glia.23454
  • Peer Reviewed
• [Journal Article] Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity. (2017)
  • Author(s): Akiyo Natsubori, Iku Tsustui-Kimura, Hiroshi Nishida, Youcef Bouchekioua, Hiroshi Sekiya, Motokazu Uchigashima, Masahiko Watanabe, Alban de Kerchove d'Exaerde, Masaru Mimura, Norio Takata, and Kenji Tanaka
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 10 Pages: 2723-2733
  • DOI: 10.1523/jneurosci.3377-16.2017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dysfunction of ventrolateral striatal dopamine receptor type 2-expressing medium spiny neurons impairs instrumental motivation. (2017)
  • Author(s): Tsutsui-Kimura I, Takiue H, Yoshida K, Xu M, Yano R, Ohta H, Nishida H, Bouchekioua Y, Okano H, Uchigashima M, Watanabe M, Takata N, Drew MR, Sano H, Mimura M, Tanaka KF.
  • Journal Title: Nat Commun. Volume: 8 Issue: 1 Pages: 14304-14304
  • DOI: 10.1038/ncomms14304
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe (2016)
  • Author(s): Keitaro Yoshida, Yu Mimura, Ryosuke Ishihara, Hiroshi Nishida, Yuji Komaki, Tomohito Minakuchi, Tomokazu Tsurugizawa, Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka, Norio Takata
  • Journal Title: J Neurosci Methods. Volume: 274 Pages: 38-48
  • DOI: 10.1016/j.jneumeth.2016.09.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Astroglial Glutamate Transporter Deficiency Increases Synaptic Excitability and Leads to Pathological Repetitive Behaviors in Mice. (2015)
  • Author(s): Aida T, Yoshida J, Nomura M, Tanimura A, Iino Y, Soma M, Bai N, Ito Y, Cui W, Aizawa H, Yanagisawa M, Nagai T, Takata N, Tanaka KF, Takayanagi R, Kano M, Gotz M, Hirase H, Tanaka K.
  • Journal Title: Neuropsychopharmacology Volume: in press Issue: 7 Pages: 1660-1660
  • DOI: 10.1038/npp.2015.26
  • Peer Reviewed / Open Access
• [Journal Article] Optogenetic Activation of CA1 Pyramidal Neurons at the Dorsal and Ventral Hippocampus Evokes Distinct Brain-Wide Responses Revealed by Mouse fMRI. (2015)
  • Author(s): N, Yoshida K, Komaki Y, Xu M, Sakai Y, Hikishima K, Mimura M, Okano H, Tanaka KF.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: e0121417-e0121417
  • DOI: 10.1371/journal.pone.0121417
  • Peer Reviewed / Open Access
• [Journal Article] Adaptive changes of extracellular amino acid concentrations in mouse dorsal striatum by 4-AP-induced cortical seizures (2015)
  • Author(s): Terumi Nagai, Norio Takata, Yoshiaki Shinohara, Hajime Hirase
  • Journal Title: Neuroscience Volume: 295 Pages: 229-236
  • DOI: 10.1016/j.neuroscience.2015.03.043
  • NAID: 120006386964
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of the extent of cortical spreading depression propagation by Npas4 mRNA expression (2015)
  • Author(s): Keitaro Yoshida, Ming Xu, Akiyo Natsubori, Masaru Mimura, Norio Takata, Kenji F Tanaka
  • Journal Title: Neuroscinece Research Volume: - Pages: 1-8
  • DOI: 10.1016/j.neures.2015.04.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Observation and manipulation of glial cell function by virtue of sufficient probe expression (2015)
  • Author(s): Akiyo Natsubori, Norio Takata, Kenji F Tanaka
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 176 Pages: 1-7
  • DOI: 10.3389/fncel.2015.00176
  • Peer Reviewed / Open Access
• [Journal Article] Neuronal heterotopias affect the activities of distant brain areas and lead to behavioral deficits (2015)
  • Author(s): Kazuhiro Ishii, Ken-ichiro Kubo, Toshihiro Endo, Keitaro Yoshida, Seico Benner, Yukiko Ito, Hidenori Aizawa, Michihiko Aramaki, Akihiro Yamanaka, Kohichi Tanaka, Norio Takata, Kenji Tanaka, Masaru Mimura, Chiahru Tohyama, Masaki Kakeyama, and Kazunori Nakajima
  • Journal Title: Journal of Neuroscience Volume: 35 Issue: 36 Pages: 12432-45
  • DOI: 10.1523/jneurosci.3648-14.2015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 光遺伝学的fMRIを用いた海馬活動伝播様式の全脳解析 (2017)
  • Author(s): 高田則雄
  • Organizer: 次世代プロジェクト「異なる時期の虚血が脳の発達に及ぼす影響の多層的アプローチによる解明と治療戦略構築」国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Dissection of downstream targets of the hippocampus using optogenetic fMRI (2017)
  • Author(s): 高田則雄
  • Organizer: 次世代プロジェクト「異なる時期の虚血が脳の発達に及ぼす影響の多層的アプローチによる解明と治療戦略構築」国際シンポジウム
  • Place of Presentation: 慶応義塾大学医学部
  • Int'l Joint Research / Invited
• [Presentation] 光遺伝学的fMRIを用いたアストロサイトによるBOLD信号発生の研究 (2016)
  • Author(s): 高田則雄
  • Organizer: 第89回日本薬理学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-09
  • Invited
• [Presentation] Optogenetic-fMRI for investigation of neuronal and glial contribution to BOLD signal (2015)
  • Author(s): 高田則雄
  • Organizer: 第38回日本神経科学会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-07-29
  • Invited