Morphogenesis of endoplasmic reticulum through self-organization in plant cells

• Project/Area Number: 15KT0151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Constructive Systems Biology
• Research Institution: Konan University (2016-2017); Kyoto University (2015)
• Principal Investigator: Haruko Ueda 甲南大学, 理工学部, 特任研究准教授 (90402776)
• Project Period (FY): 2015-07-10 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 小胞体 / シロイヌナズナ

Outline of Final Research Achievements

The endoplasmic reticulum (ER) is a membrane-enclosed organelle in eukaryotes. The ER develops a continuous network composed of interconnected membrane tubules and flat cisternae called ER sheets in the cell periphery. The network structure is widely conserved in eukaryotes. The multifunctionality of the ER is thought to be closely related to its morphology. However, the mechanism of the ER morphogenesis in plant cells are largely unknown. In this study, we identified two ER membrane protein families, which are required for the ER morphogenesis in plant cells.

Research Products

• [Journal Article] FAMA: A Molecular Link between Stomata and Myrosin Cells. (2016)
  • Author(s): Shirakawa M, Ueda H, Shimada T, Hara-Nishimura I.
  • Journal Title: Trends Plant Sci. Volume: 21 Issue: 10 Pages: 861-871
  • DOI: 10.1016/j.tplants.2016.07.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis. (2016)
  • Author(s): Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
  • Journal Title: Plant Physiol. Volume: 170 Issue: 2 Pages: 867-880
  • DOI: 10.1104/pp.15.01172
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Myrosin cells are differentiated directly from ground meristem cells and are developmentally independent of the vasculature in Arabidopsis leaves (2016)
  • Author(s): Shirakawa, M., Ueda, H., Shimada, T., Hara-Nishimura, I.
  • Journal Title: Plant Signal. Behav. Volume: 11 Issue: 4 Pages: e1150403-e1150403
  • DOI: 10.1080/15592324.2016.1150403
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functions of plant-specific myosin XI: from intracellular motility to plant postures. (2015)
  • Author(s): Ueda, H., Tamura, K., Hara-Nishimura, I.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 28 Pages: 30-38
  • DOI: 10.1016/j.pbi.2015.08.006
  • NAID: 120005676685
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] MAG3は小胞体とゴルジ体の境界で効率的な物質輸送に寄与する (2018)
  • Author(s): 高木純平，高橋英之，長野稔，深尾陽一朗，上田晴子，田村謙太郎，嶋田知生，西村いくこ
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 細胞膜接着部位に着目した小胞体の4次元構造解析 (2018)
  • Author(s): 石川一也，田村謙太郎，上田晴子，伊藤容子，中野明彦，西村いくこ，嶋田知生
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] ABCB19は植物器官のストレートニング機構に関与する (2018)
  • Author(s): 井田千香子，上田晴子，嶋田知生，田村謙太郎，森田(寺尾)美代，西村いくこ
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 植物の器官屈曲を抑制する復元機構 (2017)
  • Author(s): 上田晴子，西村いくこ
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島市）
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] アクチン・ミオシンXIはストレートニング機構を介して植物の姿勢を決定する (2017)
  • Author(s): 上田晴子，西村いくこ
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸国際会議場（神戸市）
  • Year and Date: 2017-01-06
• [Presentation] 小胞体膜タンパク質の機能解析 (2017)
  • Author(s): 上田晴子
  • Organizer: 第9回植物細胞研究会
• [Presentation] 植物のActin-myosin XI細胞骨格依存的なストレートニング機構 (2017)
  • Author(s): 西村いくこ，上田晴子
  • Organizer: 2017年度生命科学系学会合同年次大会 ConBio2017
  • Invited
• [Presentation] The coiled-coil protein MAG3 is involved in protein transport at the endoplasmic reticulum-Golgi interface (2017)
  • Author(s): Junpei Takagi, Hideyuki Takahashi, Minoru Nagano, Masayuki Fujiwara, Yoichiro Fukao, Haruko Ueda, Kentaro Tamura, Akihiko Nakano, Tomoo Shimada, Ikuko Hara-Nishimura
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research
• [Presentation] The membrane protein MAG3 functions in protein transport between endoplasmic reticulum and Golgi apparatus. (2017)
  • Author(s): Junpei Takagi, Hideyuki Takahashi, Minoru Nagano, Masayuki Fujiwara, Yoichiro Fukao, Haruko Ueda, Kentaro Tamura, Akihiko Nakano, Tomoo Shimada, Ikuko Hara-Nishimura
  • Organizer: 20th European Network for Plant Endomembrane Research meeting
  • Int'l Joint Research
• [Presentation] RHD3依存的な膜融合による小胞体ネットワーク形成 (2016)
  • Author(s): 上田晴子
  • Organizer: 植物細胞骨格研究会2016
  • Place of Presentation: 東京大学駒場キャンパス（東京）
  • Year and Date: 2016-11-18
• [Presentation] 重力・光刺激に応答した植物の器官屈曲とストレートニング機構 (2016)
  • Author(s): 上田晴子
  • Organizer: 日本宇宙生物科学会第30回大会
  • Place of Presentation: 愛知医科大学（長久手市）
  • Year and Date: 2016-10-14
  • Invited
• [Presentation] Functions of an actin-myosin XI cytoskeleton ~ from intracellular motility to plant organ movement ~ (2016)
  • Author(s): Haruko Ueda, Ikuko Hara-Nishimura
  • Organizer: Front Lines of Plant Cell Wall Research and Beyond
  • Place of Presentation: KKRホテル熱海（熱海市）
  • Year and Date: 2016-10-04
  • Int'l Joint Research / Invited
• [Presentation] リン酸化を介した小胞体膜融合因子RHD3の活性調節 (2016)
  • Author(s): 上田晴子、横田悦雄、桑田啓子、真野昌二、嶋田知生、田村謙太郎、深尾陽一朗、新免輝男、西村幹夫、西村いくこ
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-20
• [Presentation] アクチン・ミオシンXI依存的なストレートニング機構の役割 (2016)
  • Author(s): 上田晴子，岡本圭史，嶋田知生，田村謙太郎，加藤荘英，田坂昌生，森田美代，西村いくこ
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-19
• [Presentation] An Analysis of Transport of Glucosinolates in Arabidopsis Leaves. (2016)
  • Author(s): 中崎淳子、山田健志、河本恭子、上田晴子、西村いくこ、嶋田知生
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-19
• [Presentation] Chemical defense systems in Brassicaceae plants (2016)
  • Author(s): Hara-Nishimura, I., Yamada, K., Shirakawa, M., Nakano, R., Ueda, H., Shimada, T.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Flavonoid accumulation in Arabidopsis seeds affected by GFS9-mediated membrane trafficking (2016)
  • Author(s): Ichino, T., Fuji, K., Ueda, H., Takahashi, H., Koumoto, Y., Takagi, J., Tamura, K., Sasaki, R., Aoki, K., Schumacher, K., Shimada, T., Hara-Nishimura, I.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Abscission of the outermost cell layer of Arabidopsis root cap is controlled by modification of cell wall pectin (2016)
  • Author(s): 前田和輝、國枝正、上田晴子、嶋田知生、西村いくこ
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Active movements and network formation of the endoplasmic reticulum in plant cells (2015)
  • Author(s): Ueda, H., Yokota, E.
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（神戸市）
  • Year and Date: 2015-12-04
  • Int'l Joint Research / Invited
• [Presentation] An actin-myosin XI cytoskeleton determines plant posture by regulating organ straightening. (2015)
  • Author(s): Ueda H., Shimada, T., Tamura, K., Terao Morita, M., Hara-Nishimura, I.
  • Organizer: 8th Plant Biomechanics International Conference
  • Place of Presentation: 名古屋大学（名古屋市）
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] 植物の形態におけるミオシンXIの役割 (2015)
  • Author(s): 上田晴子
  • Organizer: 植物細胞骨格研究会2015
  • Place of Presentation: 国立遺伝学研究所（三島市）
  • Year and Date: 2015-11-27