| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Various lipids and membrane are assumed as candidates for molecules that define the identity of cell membranes such as plasma membrane and organelles. However, it is difficult to determine the minimal combination of lipids and membrane proteins which define specific cell membrane by conventional cell biological approaches. Therefore, we tried to establish a method to introduce liposomes and proteins into cells in this research project. As a result, we established a method to introduce liposome and protein complex encapsulated in giant liposomes into cells by fusing cells with giant liposomes by electroporation.
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