Exploring of the principle of epithelial tubulogenesis by intra- and extracellular small molecules

• Project/Area Number: 15KT0154
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Constructive Systems Biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: SUZUKI MAKOTO 基礎生物学研究所, 形態形成研究部門, 助教 (10533193)
• Co-Investigator(Renkei-kenkyūsha): Ueno Naoto 基礎生物学研究所, 形態形成研究部門, 教授 (40221105) ; KOYAMA Hiroshi 基礎生物学研究所, 初期発生研究部門, 助教 (10530462) ; KATO Kagayaki 大学共同利用機関法人自然科学研究機構, 新分野創成センター, 特任助教 (30391915)
• Research Collaborator: YASUE Naoko
• Project Period (FY): 2015-07-10 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: カルシウムイオン / 神経管形成 / 頂端収縮 / アフリカツメガエル / 光操作 / ツメガエル / 細胞外ATP

Outline of Final Research Achievements

In this study, we explored the function of intracellular calcium ions in epithelial morphogenesis by using the neural tube closure of the African clawed frog as a model. As a result, we developed the technique that induces one of the cellular movements necessary for epithelial sheet deformation, called apical constriction, and revealed the molecular mechanism of it. Moreover, we proposed the mechanical basis by which the synchrony of the apical constriction affects the efficiency of tissue deformation. The results of this study are expected to contribute to the understanding of the principle of morphogenesis of various epithelial organs as well as the neural tube.

Research Products

• [Int'l Joint Research] アルバータ大学(カナダ)
• [Journal Article] Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure. (2017)
  • Author(s): Suzuki, M., Sato, M., Koyama, H., Hara, Y., Hayashi, K., Yasue, N., Imamura, H., Fujimori, T., Nagai, T., Campbell, RE., Ueno, N.
  • Journal Title: Development. Volume: 144 Issue: 7 Pages: 1307-1316
  • DOI: 10.1242/dev.141952
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanical regulation of three-dimensional epithelial fold pattern formation in the mouse oviduct (2016)
  • Author(s): Koyama, H., Shi, D., Suzuki, M., Ueno, N., Uemura, T., Fujimori, T.
  • Journal Title: Biophysical Journal Volume: 111 Issue: 3 Pages: 650-665
  • DOI: 10.1016/j.bpj.2016.06.032
  • Peer Reviewed
• [Journal Article] Mechanical roles of apical constriction, cell elongation, and cell migration during neural tube formation in Xenopus, Biomechanics and Modeling in Mechanobiology (2016)
  • Author(s): Yasuhiro Inoue, Makoto Suzuki, Tadashi Watanabe, Naoko Yasue, Itsuki Tateo, Taiji Adachi, Naoto Ueno
  • Journal Title: Biomechanics and Modeling in Mechanobiology Volume: 15 Issue: 6 Pages: 1733-1746
  • DOI: 10.1007/s10237-016-0794-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels. (2016)
  • Author(s): Nagasaka, A., Shinoda, T., Kawaue, T., Suzuki, M., Nagayama, K., Matsumoto, T., Ueno, N., Kawaguchi, A. and Miyata, T.
  • Journal Title: Front Cell Dev. Biol. Volume: 25 Pages: 139-139
  • DOI: 10.3389/fcell.2016.00139
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 動物発生のバイオメカニクス (2017)
  • Author(s): 上野直人, 鈴木誠, 根岸剛文.
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウインクあいち（愛知県・名古屋市）
  • Year and Date: 2017-01-19
  • Invited
• [Presentation] Periodic actomyosin contractility contributes to convergence movement during neurulation in zebrafish (2017)
  • Author(s): Makoto Suzuki, Naoto Ueno.
  • Organizer: 18th International Congress of Developmental Biology
  • Int'l Joint Research
• [Presentation] Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure (2017)
  • Author(s): Makoto Suzuki, Masanao Sato, Hiroshi Koyama, Naoto Ueno.
  • Organizer: 第27回日本数理生物学会年会
  • Invited
• [Presentation] Distinct intracellular calcium ion dynamics regulate apical constriction and differentially con- tribute to neural tube closure (2016)
  • Author(s): Suzuki, M., Ueno, N.
  • Organizer: The 22nd International Congress of Zoology (OIST, Okinawa)
  • Place of Presentation: 沖縄科学技術大学院大学（沖縄県・恩納村）
  • Int'l Joint Research / Invited