| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this research, we investigated the mechanism of spindle formation and maintenance that appears during cell division as a model, filling the gap between fluctuation in the number of individual molecules and high stability/reproducibility of the entire structure. We also aimed at revealing the design principles of the each component for forming both stable and dynamic structures. On the way, we changed the research plan: we focused on bidirectional kinesin-5, which reverses the direction of movement according to the number of molecules, and studied the motility while crosslinking microtubules. As a result, we succeeded in producing multi-molecule complexes of kinesin-5 motors using DNA nanostructures as templates, which enables us to study the relationship between the directionality reversal and the number of molecules involved.
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