Structural study of signal transduction through membrane proteins, channels and receptors

• Project/Area Number: 16001005
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: FUJIYOSHI Yoshinori Kyoto University, 大学院・理学研究科, 教授 (80142298)
• Co-Investigator(Kenkyū-buntansha): MIZOGUCHI Akira 三重大学, 医学部, 教授 (90181916) ; YASUI Masato 慶應義塾大学, 医学部, 教授 (90246637)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥547,560,000 (Direct Cost: ¥421,200,000、Indirect Cost: ¥126,360,000); Fiscal Year 2008: ¥60,840,000 (Direct Cost: ¥46,800,000、Indirect Cost: ¥14,040,000); Fiscal Year 2007: ¥91,260,000 (Direct Cost: ¥70,200,000、Indirect Cost: ¥21,060,000); Fiscal Year 2006: ¥111,540,000 (Direct Cost: ¥85,800,000、Indirect Cost: ¥25,740,000); Fiscal Year 2005: ¥131,820,000 (Direct Cost: ¥101,400,000、Indirect Cost: ¥30,420,000); Fiscal Year 2004: ¥152,100,000 (Direct Cost: ¥117,000,000、Indirect Cost: ¥35,100,000)
• Keywords: 構造生物学 / シグナル伝達 / 神経科学 / 生理学 / 脳・神経 / 水チャネル / イオンチャネル / Gタンパク質共役型受容体 / 細胞接着 / シナプス / 電子線結晶学 / 2次元結晶 / プロテオミクス

Research Abstract

膜蛋白質を中心とする情報伝達と制御の分子機構を構造の視点から理解することを目的として、具体的には、1) 水チャネルの構造と機能、活性制御、そして高次機能の研究、2) イオンチャネルの構造と機能解析および局在化機構等による高次機能の解析、3) エンドセリン受容体B型(ETBR)等のG蛋白質共役型受容体(GPCR)の構造と機能解析の3つの研究課題を遂行した。

Research Products

• [Journal Article] Mechanism of Aquaporin-4's Fast and Highly Selective Water Conduction and Proton Exclusion. (2009)
  • Author(s): K. Tani, T. Mitsuma, Y. Hiroaki, A. Kamegawa, K. Nishikawa, Y. Tanimura and Y. Fujiyoshi
  • Journal Title: J. Mol. Biol. 389 Pages: 694-706
  • Peer Reviewed
• [Journal Article] Inter-subunit interaction of gastric H^+, K^+-ATPase prevents reverse reaction of the transport cycle. (2009)
  • Author(s): K. Abe, K. Tani, T. Nishizawa and Y. Fujiyoshi
  • Journal Title: EMBO J. (in the press)
  • Peer Reviewed
• [Journal Article] Structure of the connexin-26 gap junction channel at 3.5 Å resolution. (2009)
  • Author(s): S. Maeda, S. Nakagawa, M. Suga, E. Yamashita, A. Oshima, Y. Fujiyoshi and T. Tsukihara
  • Journal Title: Nature 458 Pages: 597-602
  • Peer Reviewed
• [Journal Article] Acetazolamide reversibly inhibits water conduction by aquaporin-4. (2009)
  • Author(s): Y. Tanimura, Y. Hiroaki and Y. Fujiyoshi
  • Journal Title: J. Struct. Biol. 166 Pages: 16-21
  • Peer Reviewed
• [Journal Article] Reconstruction of the P2X2 Receptor Reveals a Vase-ShapedStructure with Lateral Tunnels above the Membrane (2009)
  • Author(s): K. Mio
  • Journal Title: Structure 17 Pages: 266-275
  • Peer Reviewed
• [Journal Article] Increased susceptibility to spontaneous lung cancer inmice lacking LIM-domain only 7 (2009)
  • Author(s): M. Tanaka-Okamoto
  • Journal Title: Cancer Sci 100 Pages: 608-616
  • Peer Reviewed
• [Journal Article] Acetazolamide reversibly inhibits water conduction byaquaporin-4 (2009)
  • Author(s): Y. Tanimura
  • Journal Title: J. Struct. Biol 166 Pages: 16-21
  • Peer Reviewed
• [Journal Article] Structure of the connexin-26 gap junction channel at 3.5A resolution (2009)
  • Author(s): S. Maeda
  • Journal Title: Nature 458 Pages: 597-602
  • Peer Reviewed
• [Journal Article] Rabll and its effector Ripil participate in regulationof insulin granule exocytosis (2009)
  • Author(s): k. Sugawara
  • Journal Title: Genes Cells 14 Pages: 445-456
  • Peer Reviewed
• [Journal Article] Unusual thetmal Aisassembly of the SPFH domain oligomerfrom Nrocoecus horiAvshil (2009)
  • Author(s): Y. Kuwahara
  • Journal Title: Biophys. J.
  • Peer Reviewed
• [Journal Article] Inter-subunit interaction of gastric H^+, K^+-ATPaseprevents reverse reaction of the transport cycle (2009)
  • Author(s): K. Abe
  • Journal Title: EMBO J.
  • Peer Reviewed
• [Journal Article] 22. Molecular mechanism of fast and high water selectiveaquaporin-4 channel (2009)
  • Author(s): K. Tani
  • Journal Title: J. Mol. Biol
  • Peer Reviewed
• [Journal Article] Formation of aquaporin-4 arrays is inhibited by palmitoylation of N-terminal cysteine residues. (2008)
  • Author(s): H. Suzuki, K. Nishikawa, Y. Hiroaki and Y. Fujiyoshi
  • Journal Title: Biochem. Biophys. Acta. 1778 Pages: 1181-1189
  • Peer Reviewed
• [Journal Article] Projection Structure of a N-terminal Deletion Mutant of Connexin 26 Channel with Decreased Central Pore Density (2008)
  • Author(s): A Oshima
  • Journal Title: Cell Commun. Adhes 15 Pages: 85-93
  • Peer Reviewed
• [Journal Article] Neuromyelitis optica and anti-aquaporin-4 antibodiesmeasured by an enzyme-linked immunosorbent assay (2008)
  • Author(s): S. Hayakawa
  • Journal Title: J. Neuroimmun 196 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Sendai virus F glycoprotein induces IL-6 production in dendritic cells in a fusion-independent manner (2008)
  • Author(s): H. Suzuki
  • Journal Title: FEBS Lett 582 Pages: 1325-1329
  • Peer Reviewed
• [Journal Article] Mercury chloride decreases the water permeability of aquaporin-4-reconstituted proteoliposomes (2008)
  • Author(s): Y. Yukutake
  • Journal Title: Biol. Cell 100 Pages: 355-363
  • Peer Reviewed
• [Journal Article] 膜タンパク質の構造から見たチャネルの分子機構 (2008)
  • Author(s): 藤吉好則
  • Journal Title: 固体物理 43 Pages: 35-44
• [Journal Article] PASK (proline-alanine-rich Ste20-related kinase) binds to tubulin andmicrotubules and is involved in microtubule stabilization (2008)
  • Author(s): T. Tsutsumi
  • Journal Title: Arch. Biochem. Biophys 15 Pages: 267-278
  • Peer Reviewed
• [Journal Article] Electron crystallography of proteins in membranes (2008)
  • Author(s): Y. Fuj iyoshi
  • Journal Title: Current Opinion in Structural Biology 18 Pages: 587-592
  • Peer Reviewed
• [Journal Article] Dual inhibition of SNARE complex formation by tomosynensures controlled neurotransmitter release (2008)
  • Author(s): T. Sakisaka
  • Journal Title: J. Cell Biol 183 Pages: 323-337
  • Peer Reviewed
• [Journal Article] Pleomorphic configuration of the trimeric capsid proteins of Rice dwarf virusthat allows formation of both the outer capsid and tubular crystals (2008)
  • Author(s): K. Iwasaki
  • Journal Title: J. Mol. Biol 383 Pages: 252-265
  • Peer Reviewed
• [Journal Article] アクアポリン-4 (2008)
  • Author(s): 谷一寿
  • Journal Title: 臨床神経学 48 Pages: 941-944
• [Journal Article] Phosphorylation in the C-terminal domain of Aquaporin-4 isrequired for Golgi ransition in primary cultured astrocytes (2008)
  • Author(s): I. Kadohira
  • Journal Title: Biochem. Biophys. Res. Commun 377 Pages: 463-468
  • Peer Reviewed
• [Journal Article] Establishment of cell polarity by afadin during the formation of embryoid bodies. (2008)
  • Author(s): H. Komura
  • Journal Title: Genes Cells 13 Pages: 79-90
  • Peer Reviewed
• [Journal Article] Formation of aquaporin-4 arrays is inhibited by palmitoylation of N-terminal cysteine residues. (2008)
  • Author(s): H. Suzuki
  • Journal Title: Biochem. Biophys. Acta. 1778 Pages: 1181-1189
  • Peer Reviewed
• [Journal Article] Junction-forming aquaporins. (2008)
  • Author(s): A. Engel
  • Journal Title: Current Opinion in Structural Biology 18 Pages: 229-235
  • Peer Reviewed
• [Journal Article] Structural analysis of 2D crystals of gastric H^+, K^+-ATPase in different states of the transport cycle. (2008)
  • Author(s): T. Nishizawa
  • Journal Title: J. Structural. Biol. (In press)
  • Peer Reviewed
• [Journal Article] Mercury Chloride Decreases the Water Permeability of Aaquaporin-4-Reconstituted Proteoliposomes. (2008)
  • Author(s): Y. Yukutake
  • Journal Title: Biol. Cell (In press)
  • Peer Reviewed
• [Journal Article] Projection Structure of a N-terminal Deletion Mutant of Connexin 26 Channel with Decreased Central Pore (2008)
  • Author(s): A. Oshima
  • Journal Title: Cell Communication & Adhesion (In press)
  • Peer Reviewed
• [Journal Article] Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule. (2007)
  • Author(s): A. Oshima, K. Tani, Y. Hiroaki, Y. Fujiyoshi and G. E. Sosinsky
  • Journal Title: PNAS 104 Pages: 10034-10039
  • Peer Reviewed
• [Journal Article] Aquaporin-11 containing a divergent NPA motif has normal water channel activity. (2007)
  • Author(s): K. Yakata, Y. Hiroaki, K. Ishibashi, E. Sohara, S. Sasaki, K. Mitsuoka and Y. Fujiyoshi
  • Journal Title: Biochim. Biophys. Acta. 1768 Pages: 688-693
  • Peer Reviewed
• [Journal Article] TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation. (2007)
  • Author(s): Y. Toiyama
  • Journal Title: World J. Gastroenterol. 13 Pages: 2717-2721
  • Peer Reviewed
• [Journal Article] Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule. (2007)
  • Author(s): A. Oshima
  • Journal Title: PNAS 104 Pages: 10034-10039
  • Peer Reviewed
• [Journal Article] S-SCAM{alpha} is a Scaffold to Mediate NMDA Receptor-dependent RhoA Activation in Dendrites. (2007)
  • Author(s): J. Iida
  • Journal Title: Mol. Cell. Biol. 27 Pages: 4388-4405
  • Peer Reviewed
• [Journal Article] Diminished climbing fiber innervation of Purkinje cells in the cerebellum of myosin Va mutant mice and rats. (2007)
  • Author(s): Y. Takagishi
  • Journal Title: Dev. Neurobiol. 67 Pages: 909-923
  • Peer Reviewed
• [Journal Article] Simulation of charge effects on density maps obtained by high-resolution electron crystallography. (2007)
  • Author(s): T. Hirai
  • Journal Title: J. Electron Microsc. 56 Pages: 131-140
  • Peer Reviewed
• [Journal Article] A nuclear factor-kappaB inhibitor pyrrolidine dithiocarbamate ameliorates pulmonary hypertension in rats. (2007)
  • Author(s): H. Sawada
  • Journal Title: CHEST 132 Pages: 2717-2721
  • Peer Reviewed
• [Journal Article] Activity-induced protocadherin arcadlin regulates dendritic spine number by triggering N-cadherin endocytosis via TA02beta and p38 MAP kinases. (2007)
  • Author(s): S. Yasuda
  • Journal Title: Neuron 56 Pages: 456-471
  • Peer Reviewed
• [Journal Article] Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. (2007)
  • Author(s): M. Toei
  • Journal Title: PNAS 104 Pages: 20256-20261
  • Peer Reviewed
• [Journal Article] Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer. (2007)
  • Author(s): Y.Okada
  • Journal Title: Nat.Biotechnol. 25・2 Pages: 233-237
• [Journal Article] Aquaporin-11 containing a divergent NPA motif has normal water channel activity. (2007)
  • Author(s): K.Yakata
  • Journal Title: Biochim.Biophys.Acta. 1768・3 Pages: 688-693
• [Journal Article] Impaired cerebellar development and function in mice lacking CAPS2, a protein involved in neurotrophin release. (2007)
  • Author(s): T.Sadakata
  • Journal Title: J.Neurosci. 27・10 Pages: 2472-2482
• [Journal Article] The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas. (2007)
  • Author(s): K.Mio
  • Journal Title: J.Mol.Biol. 367・2 Pages: 373-383
• [Journal Article] Neurosteroid pregnenolone sulfate enhances glutamatergic synaptic transmission by facilitating presynaptic calcium currents at the calyx of Held of immature rats. (2006)
  • Author(s): T. Hige, Y. Fujiyoshi and T. Takahashi
  • Journal Title: Eur. J. Neurosci. 24 Pages: 1955-1966
  • Peer Reviewed
• [Journal Article] Implications of the Aquaporin-4 Structure on Array Formation and Cell Adhesion. (2006)
  • Author(s): Y. Hiroaki, K. Tani, A. Kamegawa, N. Gyobu, K. Nishikawa, H. Suzuki, T. Walz, S. Sasaki, K. Mitsuoka, K. Kimura, A. Mizoguchi and Y. Fujiyoshi
  • Journal Title: J. Mol. Biol. 355 Pages: 628-639
  • Peer Reviewed
• [Journal Article] Differential localization and regulation of stargazin-like protein, gamma-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. (2006)
  • Author(s): M.Inamura
  • Journal Title: Neurosci Res. 55・1 Pages: 45-53
• [Journal Article] Structural Basis for Detoxification and Oxidative Stress Protection in Membranes. (2006)
  • Author(s): P.J.Holm
  • Journal Title: J.Mol.Biol. 360・5 Pages: 934-945
• [Journal Article] Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart. (2006)
  • Author(s): R.Okamoto
  • Journal Title: Cell.Signal. 18・9 Pages: 1408-1416
• [Journal Article] Neurosteroid pregnenolone sulfate enhances glutamatergic synaptic transmission by facilitating presynaptic calcium currents at the calyx of Held of immature rats. (2006)
  • Author(s): T.Hige
  • Journal Title: Eur.J.Neurosci. 24・7 Pages: 1955-1966
• [Journal Article] RGS2 promotes formation of neurites by stimulating microtubule polymerization. (2006)
  • Author(s): K.Heo
  • Journal Title: Cell.Signal. 18・12 Pages: 2182-2192
• [Journal Article] Protruding disordered loop of gClqR is specifically exposed and related to antiapoptotic property in germ cell lineage. (2006)
  • Author(s): S.Kitazawa
  • Journal Title: Histochem.Cell Biol. 126・6 Pages: 665-677
• [Journal Article] Choroid plexus ependymal cells host neural progenitor cells in the rat. (2006)
  • Author(s): Y.Itokazu
  • Journal Title: Glia. 53・1 Pages: 32-42
• [Journal Article] Essential Contribution of the Ligand-Binding βB/βC Loop of PDZ1 and PDZ2 in the Regulation of Postsynaptic Clustering, Scaffolding, and Localization of Postsynaptic Density-95. (2006)
  • Author(s): M.Nonaka
  • Journal Title: J.Neurosci. 26・3 Pages: 763-774
• [Journal Article] Implications of the Aquaporin-4 Structure on Array Formation and Cell Adhesion. (2006)
  • Author(s): Y.Hiroaki
  • Journal Title: J.Mol.Biol. 355・4 Pages: 628-639
• [Journal Article] Involvement of nectins in the formation of puncta adherentia junctions and the mossy fiber trajectory in the mouse hippocampus. (2006)
  • Author(s): T.Honda
  • Journal Title: Mol.Cell.Neurosci. 31・2 Pages: 315-325
• [Journal Article] Two-dimensional crystallization and analysis of projection images of intact Thermus thermophilus V-ATPase. (2006)
  • Author(s): C.Gerle
  • Journal Title: J.Structural.Biol. 153・2 Pages: 200-206
• [Journal Article] Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart. (2006)
  • Author(s): R.Okamoto
  • Journal Title: Cell.Signal. (In press)
• [Journal Article] Differential localization and regulation of stargazin-like protein, gamma-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. (2006)
  • Author(s): M.Inamura
  • Journal Title: Neurosci.Res. (In press)
• [Journal Article] Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. (2005)
  • Author(s): T. Gonen, Y. Cheng, P. Sliz, Y. Hiroaki, Y. Fujiyoshi, S. C. Harrison and T. Walz
  • Journal Title: Nature 438 Pages: 633-638
  • Peer Reviewed
• [Journal Article] Nectin-like molecule 1/TSLL1/SynCAM3 : a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non junctional contact sites of presynaptic nerve terminals, axons and glia cell processes. (2005)
  • Author(s): S.Kakunaga
  • Journal Title: J.Cell Sci. 118・6 Pages: 1267-1277
• [Journal Article] Persistence of gene expression changes in noninflamed and inflamed colonic mucosa in ulcerative colitis and their presence in colonic carcinoma. (2005)
  • Author(s): Y.Toiyama
  • Journal Title: World J.Gastroenterol. 11・33 Pages: 5151-5155
• [Journal Article] Expression and localization of an exogenous G protein-coupled receptor fused with the rhodopsin C-terminal sequence in the retinal rod cells of knockin mice. (2005)
  • Author(s): T.Kodama
  • Journal Title: Experim.Eye Res. 80・6 Pages: 859-869
• [Journal Article] Electron tomography reveals diverse conformations of integrin αIIbβ3 in the active state. (2005)
  • Author(s): K.Iwasaki
  • Journal Title: J.Structural.Biol. 150・3 Pages: 259-267
• [Journal Article] 極低温電子顕微鏡で解析されたアセチルコリン受容体の構造と機能 (2005)
  • Author(s): 宮澤 淳夫
  • Journal Title: 構造生物 11・1 Pages: 31-50
• [Journal Article] Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. (2005)
  • Author(s): T.Gonen
  • Journal Title: Nature 438・7068 Pages: 633-638
• [Journal Article] 水チャネルの構造と機能 (2005)
  • Author(s): 藤吉 好則
  • Journal Title: 蛋白質 核酸 酵素 50・10 Pages: 1278-1283
• [Journal Article] Proteomic analysis revealed a novel synaptic proline-rich membrane protein (PRR7) associated with PSD-95 and NMDA receptor. (2005)
  • Author(s): Y.Murata
  • Journal Title: Biochem.Biophys.Research Communications 327・1 Pages: 183-191
• [Journal Article] The secretory granule-associated protein CAPS2 regulates neurotrophin release and cell survival. (2004)
  • Author(s): T.Sadataka
  • Journal Title: J.Neucreosci. 24・1 Pages: 43-52
• [Journal Article] Characterization and application of monoclonal antibodies against human endothelin B receptor expressed in insect cells. (2004)
  • Author(s): T.Yamaguchi
  • Journal Title: Biotechnology Letters 26・4 Pages: 293-299
• [Journal Article] Improved Specimen Preparation for Cryo-Electron Microscopy using a Symmetric Carbon Sandwich Technique. (2004)
  • Author(s): N.Gyobu
  • Journal Title: J.Structural Biol. 146・3 Pages: 325-333
• [Journal Article] Neurite outgrowth from hippocampal neurons is promoted by choroid plexus ependymal cells in vitro. (2004)
  • Author(s): K.Kimura
  • Journal Title: J.Neurocytol. 33・4 Pages: 465-476
• [Journal Article] 極低温電子顕微鏡による蛋白質の立体構造解析 (2004)
  • Author(s): 光岡 薫
  • Journal Title: 蛋白質核酸酵素 49・11 Pages: 1794-1799
• [Journal Article] A novel mode of action of an ArfGAP, AMAP2/PAG3/Papalpha, in Arf6 function. (2004)
  • Author(s): S.Hashimoto
  • Journal Title: J.Biol.Chem. 279・3 Pages: 37677-37684
• [Journal Article] Nectin-Afadin細胞接着機構 (2004)
  • Author(s): 溝口 明
  • Journal Title: 生体の科学 55・5 Pages: 438-439
• [Journal Article] Nectin-like molecule-1/TSLL1/SynCAM3 : A neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites where presynaptic nerve terminals, axons, and glia cell processes contact
  • Author(s): S.Kakunaga
  • Journal Title: J.Cell Scie. (in press)
• [Presentation] 構造生理学研究のための見る技術 (2009)
  • Author(s): 藤吉好則
  • Organizer: マイクロビームアナリシス第141委員会第135回研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-03
• [Presentation] Structure and function of adhennels : adhesive channels (2009)
  • Author(s): Y. Fujiyoshi
  • Organizer: Network of Excelence-3D-EM Final Meeting
  • Place of Presentation: Brdo (Slovenia)
  • Year and Date: 2009-02-11
• [Presentation] 膜タンパク質の構造を機能研究の現状 (2009)
  • Author(s): 藤吉好則
  • Organizer: 三井化学株式会社講演会
  • Place of Presentation: 茂原
  • Year and Date: 2009-01-19
• [Presentation] 多機能性チャネルを中心とした構造生理学 (2009)
  • Author(s): 藤吉好則
  • Organizer: 生機能研究科第46回研究交流本フロンティアバイオサイエンスコロキウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-01-14
• [Presentation] これからの生命科学、数学に求めること (2009)
  • Author(s): 藤吉好則
  • Organizer: RIMS共同研究「離散力学系の分子細胞生物学への応用数理」
  • Place of Presentation: 京都
  • Year and Date: 2009-01-05
• [Presentation] Structure and function of adhennels : adhesive channels (Invited lecture) (2009)
  • Author(s): Y. Fujiyoshi
  • Organizer: Network of Excelence-3D-EM Final Meeting
  • Place of Presentation: Brdo (Slovenia)
• [Presentation] Structure and function of Multifunctional Channels (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: International Symposium on Integrative Membrane Biology
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2008-12-18
• [Presentation] Structure and function of Multifunctional Channels (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: BMB2008
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2008-12-09
• [Presentation] 多機能性チャネルの構造生理学 (2008)
  • Author(s): 藤吉好則
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Structure and function of water and ion channels (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: 150th anniversary of Japan-France relationship
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2008-11-27
• [Presentation] Structure and function of channels (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: Innovation in Diffraction Structural Biology : Neuroscience and electron microscopy
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2008-10-27
• [Presentation] Structure and function of Multifunctional channels (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: IUCr2008
  • Place of Presentation: Osaka (Japan)
  • Year and Date: 2008-08-26
• [Presentation] 電子顕微鏡を機軸とした構造生理学 (2008)
  • Author(s): 藤吉好則
  • Organizer: 松下電器産業株式会社講演会
  • Place of Presentation: 京都
  • Year and Date: 2008-07-28
• [Presentation] Membrane protein structure (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: Beijing Workshop of Cryo-Electron Microscopy-in Structural Biology
  • Place of Presentation: Beijing (China)
  • Year and Date: 2008-07-16
• [Presentation] Introduction to cryo-electron microscopy (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: Beijing Workshop of Cryo-Electron Microscopy in Structural Biology
  • Place of Presentation: Beijing (China)
  • Year and Date: 2008-07-14
• [Presentation] Structure and Function of Multifunctional Channels (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: Neuroscience2008
  • Place of Presentation: Tokyo (Japan)
  • Year and Date: 2008-07-10
• [Presentation] 多機能性チャネルを中心とした構造生理学研究 (2008)
  • Author(s): 藤吉好則
  • Organizer: 第5回KMU研究推進セミナー
  • Place of Presentation: 金沢
  • Year and Date: 2008-06-26
• [Presentation] 多機能性チャネルの構造生理学 (2008)
  • Author(s): 藤吉好則
  • Organizer: 特別講演会「今をときめく生命科学 : 如何にして新しい概念を提唱するか」
  • Place of Presentation: 仙台
  • Year and Date: 2008-06-20
• [Presentation] Regulation, structure and function of brain aquaporins (2008)
  • Author(s): Masato Yasui
  • Organizer: 1st International Meeting on Brain Aquaporins
  • Place of Presentation: Oslo (Norway)
  • Year and Date: 2008-06-09
• [Presentation] Structural Physiology of Multifunctional Channels (2008)
  • Author(s): Y. Fuj iyoshi
  • Organizer: The 1st International Conference of the Grand Challenge to Next-Generation Integrated Nanoscience
  • Place of Presentation: Tokyo-(Japan)
  • Year and Date: 2008-06-04
• [Presentation] 電子顕微鏡法の現状と展望 (2008)
  • Author(s): 藤吉好則
  • Organizer: Biological Seminar
  • Place of Presentation: 三島
  • Year and Date: 2008-05-22
• [Presentation] 電子顕微鏡を機軸とした構造生理学 (2008)
  • Author(s): 藤吉好則
  • Organizer: 日本顕微鏡学会第64回学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2008-05-22
• [Presentation] Structure and fUnction of channels (2008)
  • Author(s): Y. Fuj iyoshi
  • Organizer: Symposium 'Frontiers in Microscopy' on theoccasion of the Titan Krios Inauguration
  • Place of Presentation: Munich (Germany)
  • Year and Date: 2008-04-15
• [Presentation] 水銀によるアクアポリンの水透過阻害メカニズムに関する研究 (2008)
  • Author(s): Y. Hirano
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-19
• [Presentation] 多機能チャネルの構造生理学 (2008)
  • Author(s): 藤吉 好則
  • Organizer: 「特定領域」マルチスケール操作によるシステム細胞工学(バイオ操作)第5回シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-03-07
• [Presentation] 多機能性膜タンパク質の生理的意味 (2008)
  • Author(s): 藤吉 好則
  • Organizer: 千里ライフサイエンスセミナー<生命機能を支える生体超分子の高次構造と機能>
  • Place of Presentation: 大阪
  • Year and Date: 2008-02-28
• [Presentation] 構造生理学研究の1例 (2008)
  • Author(s): 藤吉 好則
  • Organizer: 第32回構造生物応用研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-02-21
• [Presentation] 構造生理学研究の1例 (2008)
  • Author(s): 藤吉 好則
  • Organizer: 県立広島大学大学院総合学術研究科生命システム科学特別講義
  • Place of Presentation: 広島
  • Year and Date: 2008-01-30
• [Presentation] 電子顕微鏡による膜タンパク質の構造解析 (2008)
  • Author(s): 藤吉 好則
  • Organizer: 横浜市立大学大学院国際総合科学研究「生体超分子化学講究」
  • Place of Presentation: 横浜
  • Year and Date: 2008-01-15
• [Presentation] Structure and function of Multifunctional channels (Keynote lecture) (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: IUCr2008
  • Place of Presentation: Osaka (JAPAN)
• [Presentation] Structure and Function of Multifunctional Channels (Special Lecture) (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: Neuroscience2008 (The 31st Annual Meeting of the Japan Neuroscience Society)
  • Place of Presentation: Tokyo (JAPAN)
• [Presentation] Specimen preparation techniques for high resolutionstructural studies by cryo-EM (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: Workshop on advanced Topics in EM Structure Determination
  • Place of Presentation: La Jolla (USA)
  • Year and Date: 2007-11-11
• [Presentation] Mercury chloride decreases the water permeability of aquaporin-4 reconstituted proteoliposomes (2007)
  • Author(s): Y. Yukutake
  • Organizer: G-COE International Symposium ″Novel techniquesin aquaporin research″
  • Place of Presentation: Karolinska (Sweden)
  • Year and Date: 2007-11-08
• [Presentation] Structure and function of multifunctional channels (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: Seminar at CMBN University of Oslo
  • Place of Presentation: Oslo (Norway)
  • Year and Date: 2007-10-29
• [Presentation] Structural physiology of multifunctional channels = Struggling to study brain functions= (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: Max-Planck-Institut fur Biochemie, Moleculare Strukturbiologie
  • Place of Presentation: Martinsried (Germany)
  • Year and Date: 2007-10-10
• [Presentation] 分子動力学的手法を用いたアクアポリンに関する研究 (2007)
  • Author(s): 平野 秀典
  • Organizer: 第117回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-06
• [Presentation] Expression and localization of aquaporin l in humanamniotic fibroblasts (2007)
  • Author(s): K. Kobayashi
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (Japan)
  • Year and Date: 2007-07-15
• [Presentation] Structure and function of aquaporins (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (Japan)
  • Year and Date: 2007-07-15
• [Presentation] Functional analysis of Drosophila big brain (bib) thatis a homolog of mammalian Aquaporin-4 (AQP4) (2007)
  • Author(s): K. Tatsumi
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (Japan)
  • Year and Date: 2007-07-14
• [Presentation] Inhibition of aquaporin-4(AQP4)by mercury in reconstituted liposomes (2007)
  • Author(s): Y. Yukutake
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (Japan)
  • Year and Date: 2007-07-14
• [Presentation] Water Exclusion Mechanism of Aquaporin-1 by MD simulations (2007)
  • Author(s): Y. Hirano
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (Japan)
  • Year and Date: 2007-07-13
• [Presentation] Structure and function of channels (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: Albany 2007: The 15th Conversation
  • Place of Presentation: Albany (USA)
  • Year and Date: 2007-06-22
• [Presentation] バイオ研究に生命を吹き込んだ低温観察技術 (2007)
  • Author(s): 藤吉 好則
  • Organizer: シンポジウム「科学技術立国の礎」-日本の"見る技術"を再興する-・
  • Place of Presentation: 東京
  • Year and Date: 2007-05-10
• [Presentation] Structure and function of aquaporins (Keynote lecture) (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (JAPAN)
• [Presentation] Structural physiology of multifunctional channels (Keynote lecture) (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: Joint International Symposium "Membrane Transport as a universal Biological mechanism"
  • Place of Presentation: Kyoto (JAPAN)
• [Presentation] Structure and function of cell adhesive (2006)
  • Author(s): Y. Fujiyoshi
  • Organizer: 5th International NCCR Symposium on New Trends in Structural Biology
  • Place of Presentation: Zurich (SWITZERLAND)
• [Presentation] Structure and function of water channels (2005)
  • Author(s): Y. Fujiyoshi
  • Organizer: 4th World Congress of Cellular and Molecular Biology
  • Place of Presentation: Poitiers (FRANCE)
  • Year and Date: 2005-10-09
• [Presentation] Structure and function of various water channels (Keynote Lecture) (2005)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 2nd Bilateral Japan-UK Symposium on Structural Genomics and Proteomics
  • Place of Presentation: Yokohama (JAPAN)
• [Presentation] Structure and gating mechanism of the acetylcholine receptor (2004)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 77th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Yokohama (JAPAN)
• [Presentation] Structure and function of channels observed through an electron beam (Special Lecture) (2004)
  • Author(s): Y. Fujiyoshi
  • Organizer: 16th International Congress of the IFAA Anatomical Science 2004 -From Gene to Body-
  • Place of Presentation: Kyoto (JAPAN)
• [Book] The AQP structure and functional implications. Handb. Exp. Pharmacol., 190 (2009)
  • Author(s): T. Walz, Y. Fujiyoshi and A. Engel
  • Publisher: Springer-Verlag.
• [Book] pH Regulated Anion Permeability of Aquaporin-6. Handb. Exp. Pharmacol., 190 (2009)
  • Author(s): M. Yasui
  • Publisher: Springer-Verlag.
• [Book] Hand book of Experimental Pharmacology 190 Aauaporins (The AQP Structure and Functional Implications) (2009)
  • Author(s): Editor Eric. Beitz (T. Walz)
  • Total Pages: 424
  • Publisher: Springer-Verlag
• [Book] Hand book of Experimental Pharmacology 190 Aouaporins (pH Regulated Anion Permeability of Aquaporin-6) (2009)
  • Author(s): Editor Eric Beitz (Wasato Yasui)
  • Total Pages: 424
  • Publisher: Springer-Verlag
• [Book] 膜タンパク質のナノバイオロジー チャネルの分子構造を機能 ナノバイオロジー ナノテクノロジーによる生命科学 第4章 (2004)
  • Author(s): 光岡薫, 藤吉好則
  • Publisher: 共立出版
• [Book] ナノバイオロジー ナノテクノロジーによる生命科学(第4章) (2004)
  • Author(s): 光岡 薫
  • Publisher: 共立出版
• [Remarks]
  • URL: http://em.biophys.kyoto-u.ac.jp/
• [Patent(Industrial Property Rights)] Specimen stage-moving device for charged-particle beam system (2008)
  • Inventor(s): Yoshinori Fujiyoshi, Tomohisa Fukuda
  • Industrial Property Rights Holder: JEOL Ltd.
  • Filing Date: 2008-03-31
  • Overseas
• [Patent(Industrial Property Rights)] Specimen stage-moving device for charged-particle beam system (2008)
  • Inventor(s): Yoshinori Fujiyoshi, Tomohisa Fukuda
  • Industrial Property Rights Holder: JEOL Ltd.
  • Filing Date: 2008-03-28
  • Overseas
• [Patent(Industrial Property Rights)] 固体内部の組織学的イメージ像を観察・取得する方法 (2007)
  • Inventor(s): 溝口明, 楠木正人
  • Industrial Property Rights Holder: 三重大学
  • Industrial Property Number: 2007-129723
  • Filing Date: 2007-05-15
• [Patent(Industrial Property Rights)] 荷電粒子線装置の試料ステージ移動装置 (2007)
  • Inventor(s): 藤吉好則, 福田知久
  • Industrial Property Rights Holder: 京都大学, 日本電子(株)
  • Industrial Property Number: 2007-094862
  • Filing Date: 2007-03-30
• [Patent(Industrial Property Rights)] 固体内部の組織学的イメージ像を観察・取得する方法 (2007)
  • Inventor(s): 溝口 明、楠 正人
  • Industrial Property Rights Holder: 三重大学
  • Industrial Property Number: 2007-129723
  • Filing Date: 2007-05-15
• [Patent(Industrial Property Rights)] 荷電粒子線装置の試料ステージ移動装置 (2007)
  • Inventor(s): 藤吉 好則, 福田 知久
  • Industrial Property Rights Holder: 京都大学日本電子(株)
  • Industrial Property Number: 2007-094862
  • Filing Date: 2007-03-30
• [Patent(Industrial Property Rights)] トップエントリ式試料ステージ傾斜装置 (2005)
  • Inventor(s): 藤吉好則, 出口俊二
  • Industrial Property Rights Holder: 京都大学, 日本電子(株)
  • Industrial Property Number: 2005-205558
  • Filing Date: 2005-07-14
• [Patent(Industrial Property Rights)] トップエントリ式試料ステージ傾斜装置 (2005)
  • Inventor(s): 藤吉 好則, 出口 俊二
  • Industrial Property Rights Holder: 京都大学, 日本電子(株)
  • Industrial Property Number: 2005-205558
  • Filing Date: 2005-07-14