Regulatory mechanisms of meiosis

• Project/Area Number: 16002010
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAMOTO Masayuki The University of Tokyo, 大学院・理学系研究科, 教授 (40114706)
• Co-Investigator(Kenkyū-buntansha): TANAKA Kayoko 東京大学, 大学院・理学系研究科, 講師 (80345264) ; YAMASHITA Akira 東京大学, 大学院・理学系研究科, 助教授 (30312276) ; SATO Masamitsu 東京大学, 大学院・理学系研究科, 助教授 (50447356)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥354,770,000 (Direct Cost: ¥272,900,000、Indirect Cost: ¥81,870,000); Fiscal Year 2008: ¥55,510,000 (Direct Cost: ¥42,700,000、Indirect Cost: ¥12,810,000); Fiscal Year 2007: ¥60,320,000 (Direct Cost: ¥46,400,000、Indirect Cost: ¥13,920,000); Fiscal Year 2006: ¥61,490,000 (Direct Cost: ¥47,300,000、Indirect Cost: ¥14,190,000); Fiscal Year 2005: ¥70,980,000 (Direct Cost: ¥54,600,000、Indirect Cost: ¥16,380,000); Fiscal Year 2004: ¥106,470,000 (Direct Cost: ¥81,900,000、Indirect Cost: ¥24,570,000)
• Keywords: RNA / 細胞周期 / 減数分裂 / 分裂酵母 / 遣伝子発現 / mRNA / 選択的除去 / ポリA付加 / エキソソーム / TORキナーゼ / 遺伝子発現 / TOR複合体 / 細胞増殖 / RNA結合タンパク質 / RNA分解 / 核内構造体 / サイクリン / 第二分裂 / タンパク質分解 / 後期促進複合体

Research Abstract

分裂酵母を材料に減数分裂を制御する分子機構を解析し、減数分裂のための遺伝子のmRNAにはそれらを栄養増殖時には細胞から選択的に取り除くための目印が埋め込まれていることを発見し、またそれらが減数分裂時には減数分裂マスター制御因子Mei2pによって安定化されるメカニズムを解明した。

Research Products

• [Journal Article] Purification and characterization of a major collagenase from Streptomyces parvulus (2009)
  • Author(s): Sakurai, Yasuko
  • Journal Title: Bioscience, Biotechnology and Biochemistry 73 Pages: 21-28
  • Peer Reviewed
• [Journal Article] A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast. (2008)
  • Author(s): Kimata, Y., Trickey, M., Izawa, D., Gannon, J., Yamamoto, M., and Yamano, H.
  • Journal Title: Dev. Cell 14 Pages: 446-454
  • Peer Reviewed
• [Journal Article] TOR signaling in fission yeast. (2008)
  • Author(s): Otsubo, Y., and Yamamoto, M.
  • Journal Title: Crit. Rev. Biochem. Mol. Biol. 43 Pages: 277-283
  • Peer Reviewed
• [Journal Article] TOR signaling in fissionyeast (2008)
  • Author(s): Otsubo, Yoko
  • Journal Title: Critical Review in Biochemistry and Molecular Biology 43 Pages: 277-283
  • Peer Reviewed
• [Journal Article] A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast. (2008)
  • Author(s): Kimata, Yuu.
  • Journal Title: Developmental Cell 14 Pages: 446-454
  • Peer Reviewed
• [Journal Article] Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast. (2007)
  • Author(s): Matsuo, T., Otsubo, Y., Urano, J., Tamanoi, F., and Yamamoto, M.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 3154-3164
  • Peer Reviewed
• [Journal Article] Point mutations in TOR confer Rheb - independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells. (2007)
  • Author(s): Urano, J., Sato, T., Matsuo, T., Otsubo, Y., Yamamoto, M., and Tamanoi, F.
  • Journal Title: Proc. Natl. Acad. Sci. USA. 104 Pages: 3514-3519
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying the mitosis-meiosis decision. (2007)
  • Author(s): Harigaya, Y., and Yamamoto, M.
  • Journal Title: Chromosome Res. 15 Pages: 523-537
  • Peer Reviewed
• [Journal Article] Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes. (2007)
  • Author(s): Kohda, T. A., Tanaka, K., Konomi, M., Sato, M., Osumi, M., and Yamamoto, M.
  • Journal Title: Genes Cells 12 Pages: 155-170
  • Peer Reviewed
• [Journal Article] Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast. (2007)
  • Author(s): Matsuo, Tomohiko
  • Journal Title: Molecular and Cellular Biology 27 Pages: 3154-3164
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying the mitosis-meiosis decision. (2007)
  • Author(s): Harigaya, Yuriko
  • Journal Title: Chromosome Research 15 Pages: 523-537
• [Journal Article] Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian-TOR signaling in mammalian cells. (2007)
  • Author(s): Urano, J.
  • Journal Title: Proceedings of the National Academy of Science U.S.A. 104・9 Pages: 3514-3519
• [Journal Article] Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes. (2007)
  • Author(s): Kohda, T. A.
  • Journal Title: Genes to Cells 12・2 Pages: 155-170
• [Journal Article] Selective elimination of messenger RNA prevents an incidence of untimely meiosis. (2006)
  • Author(s): Harigaya, Y., Tanaka, H., Yamanaka, S., Tanaka, K., Watanabe, Y., Tsutsumi, C., Chikashige, Y., Hiraoka, Y., Yamashita, A., and Yamamoto, M.
  • Journal Title: Nature 442 Pages: 45-50
  • Peer Reviewed
• [Journal Article] Fission yeast Numlp is a cortical factor anchoring dynein and is essential for the horse-tail nuclear movement during meiotic prophase. (2006)
  • Author(s): Yamashita, A., and Yamamoto, M.
  • Journal Title: Genetics 173 Pages: 1187-1196
  • Peer Reviewed
• [Journal Article] The Caenorhabditis elegans homologue of Deleted in Azoospermia is involved in the sperm/oocyte switch. (2006)
  • Author(s): Otori, M., Karashima, T., and Yamamoto, M.
  • Journal Title: Mol. Biol. Cell 17 Pages: 3147-3155
  • Peer Reviewed
• [Journal Article] C. elegans CPB-3 interacts with DAZ-1 and functions in multiple steps of germline development. (2006)
  • Author(s): Hasegawa, E., Karashima, T., Sumiyoshi, E. and Yamamoto, M.
  • Journal Title: Dev. Biol. 295 Pages: 689-699
  • Peer Reviewed
• [Journal Article] Selective elimination of mRNA prevents an incidence of untimely meiosis. (2006)
  • Author(s): Harigaya, Y.
  • Journal Title: Nature 442・7089 Pages: 45-50
• [Journal Article] Fission Yeast Numlp is a cortical factor anchoring dynein and is essential for the horse-tail nuclear movement during meiotic prophase. (2006)
  • Author(s): Yamashita, A.
  • Journal Title: Genetics 173・3 Pages: 1187-1196
• [Journal Article] C. elegans CPB-3 interacts with DAZ-1 and functions in multiple steps of germline development. (2006)
  • Author(s): Hasegawa, E.
  • Journal Title: Developmental Biology 295・2 Pages: 689-699
• [Journal Article] The Caenorhabditis elegans homologue of Deleted in Azoospermia is involved in the sperm/oocyte switch. (2006)
  • Author(s): Otori, M.
  • Journal Title: Molecular Biology of the Cell 17・7 Pages: 3147-3155
• [Journal Article] Fission yeast Meslp ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p. (2005)
  • Author(s): Izawa, D., Goto, M., Yamashita, A., Yamano, H., and Yamamoto, M.
  • Journal Title: Nature 434 Pages: 529-533
  • Peer Reviewed
• [Journal Article] Hrslp/Mcp6p on the meiotic SPB organizes astral microtubule arrays for oscillatory nuclear movement. (2005)
  • Author(s): Tanaka, K., Kohda, T., Yamashita, A., Nonaka, N., and Yamamoto, M.
  • Journal Title: Curr. Biol. 15 Pages: 1479-1486
  • Peer Reviewed
• [Journal Article] The roles of fission yeast Asel in mitotic cell division, meiotic nuclear oscillation and cytokinesis checkpoint signaling. (2005)
  • Author(s): Yamashita, A., Sato, M., Fujita, A., Yamamoto, M., and Toda, T.
  • Journal Title: Mol. Biol. Cell 16 Pages: 1378-1395
  • Peer Reviewed
• [Journal Article] C. elegans DAZ-1 is expressed in proliferating germ cells and directs proper nuclear organization and cytoplasmic core formation during oogenesis. (2005)
  • Author(s): Maruyama, R., Endo, S., Sugimoto, A., and Yamamoto, M.
  • Journal Title: Dev. Biol. 277 Pages: 142-154
  • Peer Reviewed
• [Journal Article] Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman spectroscopy (2005)
  • Author(s): Huang, Y. S., Karashima, T., Yamamoto, M., and Hamaguchi, H.
  • Journal Title: Biochemistry 44 Pages: 10009-10019
  • Peer Reviewed
• [Journal Article] Hrs1p/Mcp6p on the meiotic SPB organizes astral microtubule arrays for oscillatory nuclear movement. (2005)
  • Author(s): Tanaka, K.
  • Journal Title: Current Biology 15・16 Pages: 1479-1486
• [Journal Article] Molecular-level investigation of the structure, transformation and bioactivity of single living fission yeast cells by time- and space-resolved Raman spectroscopy. (2005)
  • Author(s): Huang, Y.S.
  • Journal Title: Biochemistry 44・30 Pages: 10009-10019
• [Journal Article] 減数分裂周期における細胞周期制御機構 (2005)
  • Author(s): 伊澤 大介
  • Journal Title: 実験医学 23・9 Pages: 1404-1410
• [Journal Article] C.elegans DAZ-1 is expressed in proliferating germ cells and directs proper nuclear organization and cytoplasmic core formation during oogenesis. (2005)
  • Author(s): Maruyama, R.
  • Journal Title: Developmental Biology 277 Pages: 142-154
• [Journal Article] The roles of fission yeast Ase1 in mitotic cell division, meiotic nuclear oscillation and cytokinesis checkpoint signaling. (2005)
  • Author(s): Yamashita, A.
  • Journal Title: Molecular Biology of the Cell 16 Pages: 1378-1395
• [Journal Article] Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p. (2005)
  • Author(s): Izawa, D.
  • Journal Title: Nature 434・No.7032(印刷中)
• [Journal Article] The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. (2004)
  • Author(s): Niccoli, T., Yamashita, A., Nurse, P. and Yamamoto, M.
  • Journal Title: J. Cell Sci. 117 Pages: 5543-5556
  • Peer Reviewed
• [Journal Article] Type II platelet-activating factor-acetylhydrolase is essential for epithelial morphogenesis in Caenorhabditis elegans. (2004)
  • Author(s): Inoue, T.
  • Journal Title: Proceedings of the National Academy of Science, U.S.A 101 Pages: 13233-13238
• [Journal Article] The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. (2004)
  • Author(s): Niccoli, T.
  • Journal Title: Journal of Cell Science 117 Pages: 5543-5556
• [Journal Article] Raman spectroscopic signature of life in a living yeast cell. (2004)
  • Author(s): Huang Y.
  • Journal Title: Journal of Raman Spectroscopy 35 Pages: 525-526
• [Presentation] Regulation of mitosis-meiosis decision in fission yeast (2008)
  • Author(s): Yamamoto, Masayuki
  • Organizer: Asia-Pacific Regional S. pombe Meeting
  • Place of Presentation: Singapore
  • Year and Date: 2008-07-25
• [Presentation] Molecular basis to remove meiotic mRNAs selectively in mitotic fission yeast cells (2008)
  • Author(s): Yamamoto, Masayuki
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New London, New Hampshire, U.S.A.
  • Year and Date: 2008-06-09
• [Presentation] Regulation of mitosismeiosis decision in fission yeast. (2008)
  • Author(s): Yamamoto, M.
  • Organizer: Asia-Pacific Regional S. pombe Meeting
  • Place of Presentation: Singapore
• [Presentation] Molecular basis to remove meiotic mRNAs selectively in mitotic fission yeast cells. (2008)
  • Author(s): Yamamoto, M.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New London, New Hampshire, U.S.A.
• [Presentation] Initiation of meiosis in fission yeast. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: The 8th European Meiosis Meeting in Japan
  • Place of Presentation: Hayama, Japan
• [Presentation] Selective elimination of mRNA as a novel paradigm for the regulation of meiosis. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: The Fourth International Fission Yeast Meeting
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] Selective elimination of messenger RNA as a switch between the mitotic and meiotic cell cycles in fission yeast. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: International Symposium "Ribosomes : From Structure to Gene Expression and Beyond"
  • Place of Presentation: Irvine, California, U.S.A.
• [Presentation] Regulation of sexual development by TOR complexes in fission yeast. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: The second International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: Okinawa, Japan
• [Presentation] Selective elimination of mRNA provides a novel paradigm for the regulation of meiosis. (2006)
  • Author(s): Yamamoto, M.
  • Organizer: RNA 2006 IZU
  • Place of Presentation: Izu, Japan
• [Presentation] Molecular mechanism to eliminate meiosis-specific mRNA selectively in growing fission yeast cells. (2006)
  • Author(s): Yamamoto, M.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New London, New Hampshire, U.S.A.
• [Presentation] Selective elimination of meiotic mRNAs in mitotically growing fission yeast cells. (2006)
  • Author(s): Yamamoto, M.
  • Organizer: Cell Regulations in Division and Arrest Workshop
  • Place of Presentation: Okinawa, Japan
• [Presentation] Identification of a novel RNA-binding protein that destabilizes meiosis-specific mRNAs during mitotic growth in fission yeast. (2005)
  • Author(s): Yamamoto, M.
  • Organizer: The 7th European Meiosis Meeting
  • Place of Presentation: Madrid, Spain
• [Presentation] Novel factors regulating sexual differentiation in fission yeast. (2004)
  • Author(s): Yamamoto, M.
  • Organizer: The Third International Fission Yeast Meeting
  • Place of Presentation: San Diego, California, U.S.A.
• [Book] 「体細胞分裂周期では減数分裂特異的mRNAが選択的に除去されている」蛋白質核酸酵素 (2007)
  • Author(s): 張ヶ谷有里子, 山下朗, 田仲加代子, 山本正幸
  • Publisher: 共立出版
• [Book] 酵母のすべて (2007)
  • Author(s): 田仲 加代子
  • Publisher: シュプリンガー・ジャパン
• [Book] 「減数分裂を制御するRNA-RNA結合蛋白質」蛋白質核酸酵素 (2006)
  • Author(s): 山下朗, 張ヶ谷有里子, 山本正幸
  • Publisher: 共立出版
• [Book] 「減数分裂周期における細胞周期制御機構」実験医学 (2005)
  • Author(s): 伊澤大介, 山本正幸
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/index.html
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/index.html