| Project/Area Number |
16017243
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Mie University |
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Principal Investigator |
CHINZEI Yasuo Mie University, Faculty of Medicine, Professor, 大学院医学系研究科, 教授 (60024709)
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| Co-Investigator(Kenkyū-buntansha) |
YUDA Masao Mie University, Faculty of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (90293779)
ISHINO Iomoko Mie University, Faculty of Medicine, Research Associate, 大学院医学系研究科, 助手 (40402680)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2005: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 2004: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | malarial parasite / ookinete / sporozoite / infection mechanism / rodent malaria / Anpheline mosquito / Kupffer cell / liver infection / ハマダラカ / クッパー細胞 |
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| Research Abstract |
Malarial parasites have very complicated life cycle. There are many steps for the parasites to invade into the host tissues or cells of both vector mosquito and vertebrate animal. We are interested in molecular mechanisms of the parasite invasion of host tissues. We have analyzed parasite proteins essential for invasion into host mosquito midgut and host liver using the rodent malaria Plasmodium berghei. We established EST (expressed sequence tags) databases of ookinetes and sporozoites, selected some molecules based on certain criteria, and analyzed their function by gene targeting disruption. We have elucidated several molecules that function in parasite infection (movement, recognition, invasion, development and/or proliferation) of host. We identifiedand and named as CTRP (CS TRAP Related Protein) and MAOP (Membrane Attack Ookinete Protein) from sporozoite and ookinete stages of parasites. Both proteins have membrane attack complex /perforin domain in the molecule and function to rupture the cell membrane of Kuppfer cell in the liver sinusoidal layer and mosquito midgut cell respectively. Ookinetes and sporozoites have cellular barriers, midgut epithelial cells of mosquitoes for ookinetes and the sinusoidal layer cells in the liver for sporozoites to reach their respective infective sites. Malarial parasites use different homologous molecules, and a common conserved cell traversal
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