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Stark Spectroscopy and Reaction Dynamics in a Single Cell

Research Project

Project/Area Number 16072201
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionHokkaido University

Principal Investigator

OHTA Nobuhiro  Hokkaido University, Research Institute for Electronic Science, Professor (70113529)

Co-Investigator(Kenkyū-buntansha) NAKABAYASHI Takakazu  HOKKAIDO UNIVERSITY, Research Institute for Electronic Science, Associate Professor (30311195)
IIMORI Toshifumi  HOKKAIDO UNIVERSITY, Research Institute for Electronic Science, Assistant Professor (60360947)
KINJO Masataka  HOKKAIDO UNIVERSITY, Faculty of Advanced Life Science, Professor (70177971)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥36,900,000 (Direct Cost: ¥36,900,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2005: ¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2004: ¥16,000,000 (Direct Cost: ¥16,000,000)
KeywordsSingle Living Cell / Fluorescence Lifetime Imaging / Electric Field Effect / Time-Resolved Emission / Cell Stress / Proton-Ion Concentration / 単一細胞 / シュタルク効果 / 時間分解蛍光 / 光反応 / 時空間分解測定
Research Abstract

Fluorescence lifetime images of HeLa cells expressing GFP-tudor fusion protein have been continuously measured. The lifetime image displays a uniform distribution throughout cells; however, the cells exhibit the decrease in fluorescence lifetime after the treatment with serum-free medium and exposure to normal air. These results indicate that the fluorescence lifetime becomes shorter under the stress. The change in fluorescence lifetime in living cells has been attributed to a change in local electric field produced in the protein cavity surrounding the GFP chromophore. To investigate the electric field effects on the photoexcitation dynamics of GFP, field-induced change in fluorescence lifetime of GFP was measured in PVA, and the fluorescence lifetime of GFP is found to be reduced by an electric field, suggesting that the electric field produced inside the protein cavity affects the fluorescence lifetime of GFP.
The fluorescence lifetime images in HeLa cells as well as in Halobacterium salinarum loaded with BCECF have been measured with varying intracellular pH. The results indicate that intracellular pH of a living cell can be evaluated in vivo by fluorescence lifetime imaging (FLIM).
Aggregates of the halobacteria are effectively formed by an electric field, but the local fields in halosbacteria remain unchanged even after the formation of the aggregates.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (85 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (53 results) (of which Peer Reviewed: 3 results) Presentation (28 results) Book (4 results)

  • [Journal Article] Three-Dimensional High-resolution Microspectroscopic Study of Environment-Sensitive Photosynthetic Membranes2008

    • Author(s)
      S. Kumazaki, et al.
    • Journal Title

      Molecular Nano Dynamics, 2008, Wiley-VCH (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Three-Dimensional High-resolution Microspectroscopic Study of Environment-Sensitive Photosynthetic Membranes2008

    • Author(s)
      S. Kumazaki, et al.
    • Journal Title

      Energy from the Sun, J. Allen, E. Gantt, J. Golbeck, B. Osmond eds. (Springer) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Soluble cytochrome c-554, CycA, is not essential for photosynthetic electron transfer in Chlorobium tepidum2008

    • Author(s)
      Y. Tsukatani, et al
    • Journal Title

      FEBS Lett (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A Line-Scanning Semiconfocal Multiphoton Fluorescence Microscope with a Simultaneous Broadband Spectral Acquisition and its Application to the Study of the Thylakoid Membrane of a Cyanobacterium Anabaena PCC7120.2007

    • Author(s)
      S. Kumazaki, et al
    • Journal Title

      J. Microsc. 228

      Pages: 240-254

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Crystal Structures of CbiL, a Methyltransferase Involved in Anaerobic Vitamin B12 Biosynthesis, and its Complex with S-adenosylhomocysteine : Implications for the Reaction Mechanism2007

    • Author(s)
      K. Wada, et al.
    • Journal Title

      FEBS Journal 274

      Pages: 563-573

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A Correlation between pH and Fluorescence Lifetime of BCECF in Vivo and in Vitro2007

    • Author(s)
      T.Nakabayashi, H.Wang, K.Tsujimoto, S.Miyauchi, K.Kamo, N.Ohta
    • Journal Title

      Chem. Lett. 36

      Pages: 206-207

    • Related Report
      2006 Annual Research Report
  • [Journal Article] External Electric Field Effects on Fluorescence of Pyrene Butyric Acid Doped in a Polymer Film : Concentration and Temperature Dependence2006

    • Author(s)
      A. M. Ara, T. Iimori, T. Yoshizawa, T. Nakabayashi, ^*N. Ohta
    • Journal Title

      J. Phys. Chem. B 110

      Pages: 23669-23677

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Electric-Field-Induced Changes in Absorption and Emission Spectra of CdS Nanoparticles Doped in a Polymer Film2006

    • Author(s)
      Y. Ohara, T. Nakabayashi, K. Iwasaki, T. Torimoto, B. Ohtani, T. Hiratanai, K. Konishi, N. Ohta
    • Journal Title

      J. Phys. Chem. B 110

      Pages: 20927-20936

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Electroabsorption Spectroscopy of 6-hydroxyquinoline Doped in Polymer Films : Stark Shifts and Orientation Effects2006

    • Author(s)
      M. S. Mehata, T. Iimori, T. Yoshizawa, ^*N. Ohta
    • Journal Title

      J. Phys. Chem. A 110

      Pages: 10985-10991

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cloning and Characterization of a Novel Gene Involved in Nitrogen Fixation in Heliobacterium Chlorum : a Possible Regulatory Gene2006

    • Author(s)
      J. Enkh-Amgalan, et al.
    • Journal Title

      Arch. Microbiol. 186

      Pages: 327-337

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Crystal Structure of BchU, a Methyltransferase Involved in Bacteriochlorophyll c Biosynthesis, and its Complex with S-adenosylhomocystein : Implications for Reaction Mechanism2006

    • Author(s)
      K. Wada, et al.
    • Journal Title

      J. Mol. Biol. 360

      Pages: 839-849

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ESR Signal of the Iron-sulfur Center FX and its Function in the Homodimeric Reaction Center of Heliobacterium Modesticaldum2006

    • Author(s)
      R. Miyamoto, et al.
    • Journal Title

      Biochemistry 45

      Pages: 6306-6316

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Extemal electric field effects on fluorescence of pyrene butyric acid doped in a polymer film : Concentration and temperature dependences2006

    • Author(s)
      A.M.Ara, T.Iimori, T.Yoshizawa, T.Nakabayashi, N.Ohta
    • Journal Title

      J. Phys. Chem. B 110

      Pages: 23669-23677

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Electric-Field-Induced Changes in Absorption and Emission Spectra of CdS Nanoparticles Doped in a Polymer Film2006

    • Author(s)
      Y.Ohara, T.Nakabayashi, K.Iwasaki, T.Torimoto, O.Bunsho, T.Hiratani, K.Konishi, N.Ohta
    • Journal Title

      J. Phys. Chem. B 110 (42)

      Pages: 20927-20936

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The magnetic field effect in the presence of the electric field on fluorescence of a methylene-linked compound of pyrene and N,N-dimethylaniline doped in a polymer film2006

    • Author(s)
      E.S.Medvedev, M.Mizoguchi, N.Ohta
    • Journal Title

      J. Phys. Chem. B 110 (9)

      Pages: 3938-3941

    • Related Report
      2006 Annual Research Report
  • [Journal Article] External electric field effects on emission of a mixture of tetraphenylporphyrin and fullerene doped in a polymer film2006

    • Author(s)
      Md.Wahadoszamen, T.Nakabayashi, N.Ohta
    • Journal Title

      J. Chin. Chem. Soc. 53 (1)

      Pages: 85-92

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of electric and magnetic field on fluorescence in electron donor and acceptor pairs of pyrene and N-methylphthalimide doped in a polymer film2006

    • Author(s)
      T.Yoshizawa, M.Mizoguchi, T.Iimori, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem. Phys. 324

      Pages: 26-39

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Photo-switching behavior of CdS nanoparticles doped in a polymer film2006

    • Author(s)
      Y.Ohara, T.Nakabayashi, K.Iwasaki, T.Torimoto, B.Ohtani, N.Ohta
    • Journal Title

      C. R. Chimie 9

      Pages: 742-749

    • NAID

      120000961436

    • Related Report
      2006 Annual Research Report
  • [Journal Article] External Electric Field Effects on Absorption and Fluorescence Spectra of a Fullerene Derivative and its Mixture with Zinc-Tetraphenylporphyrin Doped in a PMMA film2006

    • Author(s)
      Md.Wahadoszamen, T.Nakabayashi, S.Kang, H.Imahori, N.Ohta
    • Journal Title

      J. Phys. Chem. B 110 (41)

      Pages: 20354-20361

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Electroabsorption spectroscopy of 6-hydroxyquinoline doped in polymer films : Stark shifts and orientation effects2006

    • Author(s)
      M.S.Mehata, T.Iimori, T.Yoshizawa, N.Ohta
    • Journal Title

      J. Phys. Chem. A 110 (38)

      Pages: 10985-10991

    • Related Report
      2006 Annual Research Report
  • [Journal Article] External electric field effects on fluorescence of perylene doped in a polymer film2006

    • Author(s)
      A.M.Ara, T.Iimori, T.Yoshizawa, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem. Phys. Lett. 427

      Pages: 322-328

    • NAID

      120000957741

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Photo-switching behavior of CdS nanoparticles doped in a polymer film2006

    • Author(s)
      Y.Ohara, T.Nakabayashi, K.Iwasaki, T.Torimoto, B.Ohtani, N.Ohta
    • Journal Title

      C.R.Chimie (in press)

    • NAID

      120000961436

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Electroabsorption spectra of a complex between tetraphenylporphyrin and fullerene in a polymer film2006

    • Author(s)
      M.Wahadoszamen, T.Nakabayashi, N.Ohta
    • Journal Title

      J.Photochem.Photobiol.A 178(2-3)

      Pages: 177-184

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of electric and magnetic field on fluorescence in electron donor and acceptor pairs of pyrene and N-methylphthalimide doped in a polymer film2006

    • Author(s)
      T.Yoshizawa, M.Mizoguchi, T.Iimori, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem.Phys. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] External electric field effects on absorption and fluorescence of anthracence-(CH2)n-naphthalene bichromophoric compounds doped in a polymer film2006

    • Author(s)
      T.Nakabayashi, Bo Wu., T.Morikawa, T.Iimori, M.B.Rubin, S.Speiser, N.Ohta
    • Journal Title

      J.Photochem.Photobiol.A 178(2-3)

      Pages: 236-241

    • Related Report
      2005 Annual Research Report
  • [Journal Article] External electric field effects on emission of a mixture of tetraphenylporphyrin and fullerene doped in a polymer film2006

    • Author(s)
      M.Wahadoszamen, T.Nakabayashi, N.Ohta
    • Journal Title

      J.Chin.Chem.Soc. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The magnetic field effect in the presence of the electric field on fluorescence of a methylene-linked compound of pyrene and N, N-dimethylaniline doped in a polymer firm2006

    • Author(s)
      E.S.Medvedev, M.Mizoguchi, N.Ohta
    • Journal Title

      J.Phys.Chem.B (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Entropy rectifies the Brownian steps of kinesin2005

    • Author(s)
      Y.Taniguchi, et al.
    • Journal Title

      Nature Chemical Biology 1

      Pages: 342-347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Different sensitivities to oxygen between two strains of the photosynthetic green sulfur bacterium Chlorobium vibrioforme NCIB 8327 with bacteriochlorophyll c and d2005

    • Author(s)
      J. Harad, et al.
    • Journal Title

      Photosynth. Res. 86

      Pages: 137-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Crystallization and preliminary X-ray diffraction study of methyltransferase BchU involved in bacteriochlorophyll c biosynthesis2005

    • Author(s)
      J. Harada, et al.
    • Journal Title

      Acta Crystallographica F61

      Pages: 712-714

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Determination of stereochemistry of bacteriochlorophyll-g_F and 8^1-hydroxy-chlorophyll -a_F from Heliobacterium modesticaldum2005

    • Author(s)
      T. Mizoguchi, et al.
    • Journal Title

      Photochem. Photobiol. 8

      Pages: 666-673

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vitro activity of C-20 methyltransferase, BchU, involved in bacteriochlorophyll c biosynthetic pathway in green sulfur bacteria2005

    • Author(s)
      J. Harada, et al.
    • Journal Title

      FEBS Lett. 579

      Pages: 1983-1987

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The role of charge-transfer excited states in the electroabsorption spectra of directly meso-meso linked porphyrin arrays2005

    • Author(s)
      Y.Matsuzaki, A.Nogami, Y.Iwaki, N.Ohta, N.Yoshida, N.Aratani, A.Osuka, K.Tanaka
    • Journal Title

      J.Phys.Chem. 109(4)

      Pages: 703-713

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Electric Field Effects on IR Absorption of a-Helical Polypeptide2005

    • Author(s)
      T.Nakabayasi, N.Ohta
    • Journal Title

      Chem.Lett. 34(8)

      Pages: 1194-1195

    • Related Report
      2005 Annual Research Report
  • [Journal Article] External electric field effects on state energy and photoexcitation dynamics of diphenylpolyenes2005

    • Author(s)
      T.Nakabayashi, M.Wahadoszamen, N.Ohta
    • Journal Title

      J.Am.Chem.Soc. 127

      Pages: 7041-7052

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Electric field induced reorientation of polar molecules in a polymethylmethacrylate film studied by electroabsorption spectroscopy2005

    • Author(s)
      E.Jalviste, N.Ohta
    • Journal Title

      Proc.SPIE. 5946

      Pages: 160-171

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spectroscopic and Excited-State Properties of Tri-9-anthrylborane II : Electroabsorption and Electrofluorescence Spectra2005

    • Author(s)
      N.Kitamura, E.Sakuda, T.Iimori, T.Yoshizawa, N.Ohta
    • Journal Title

      J.Phys.Chem.,A 109

      Pages: 7435-7441

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Remarkable temperature dependence of the electrofluorescence spectra of pyrene doped in a polymer film2005

    • Author(s)
      T.Iimori, A.M.Ara, T.Yoshizawa, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem.Phys.Lett. 402

      Pages: 206-211

    • Related Report
      2005 Annual Research Report 2004 Annual Research Report
  • [Journal Article] Time-resolved measurements of the external electric field effects on fluorescence in electron donor and acceptor pairs of N-ethylcarbazole and dimethyl terephthalate doped in a polymer film2005

    • Author(s)
      T.Iimori, T.Yoshizawa, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem.Phys. 319

      Pages: 101-110

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The role of charge-transfer excited states in the electroabsorption spectra of directly meso-meso linked porphyrin arrays2005

    • Author(s)
      Y.Matsuzaki, A.Nogami, Y.Iwaki, N.Ohta, N.Yoshida, N.Aratani, A.Osuka, K.Tanaka
    • Journal Title

      J.Phys.Chem. 109・4

      Pages: 703-713

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation : Regulation of energy transfer and ferredoxin-mediated NADP^+ reduction on the cytoplasmic side2004

    • Author(s)
      Y. Tsukatani, et al.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 51122-51130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Time-resolved measurement system of electrofluorescence spectra2004

    • Author(s)
      M.Tsushima, T.Ushizaka, N.Ohta
    • Journal Title

      Rev.Sci.Instrum. 75・2

      Pages: 479-485

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Electric field effects on photoinduced electron transfer processes of methylene-linked compounds of pyrene and N,N-dimethylaniline in a PMMA polymer film2004

    • Author(s)
      M.Tsushima, N.Ohta
    • Journal Title

      J.Chem.Phys. 120・13

      Pages: 6238-6245

    • Related Report
      2004 Annual Research Report
  • [Journal Article] External electric field effects on absorption, fluorescence and phosphorescence spectra of 4-(dimethylamino)benzonitrile in a polymer film2004

    • Author(s)
      T.Yoshizawa, Y.Iwaki, N.Osaka, T.Nakabayashi, K.A.Zachariassse, N.Ohta
    • Journal Title

      J.Phys.Chem., B 108

      Pages: 19132-19139

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Electric field effects on photoisomerization process of diphenylpolyenes doped in a polymer film as revealed by a field-induced change in fluorescence spectrum2004

    • Author(s)
      M.Wahadoszamen, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem.Phys.Lett. 387

      Pages: 124-129

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Stark spectroscopy of in idole and 3-methylindole embedded into a polymethylmethacrylate film : field induced orientation effect2004

    • Author(s)
      E.Jalviste, N.Ohta
    • Journal Title

      J.Chem.Phys. 121・10

      Pages: 4730-4739

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct measurements of the electric-field-induced change in fluorescence decay profile of pyrene doped in a polymer film2004

    • Author(s)
      T.Nakabayashi, T.Morikawa, N.Ohta
    • Journal Title

      Chem.Phys.Lett. 395

      Pages: 346-350

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 時間相関単一光子計数法と変調電場を組み合わせた時間分解電場蛍光スペクトル測定装置の製作2004

    • Author(s)
      津島稔, 牛坂健, 太田信廣
    • Journal Title

      分光研究 53・2

      Pages: 93-101

    • NAID

      10012346033

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 電場印加による発光特性変化のリアルタイム観測装置の開発2004

    • Author(s)
      太田信廣
    • Journal Title

      Material Stage 4・6

      Pages: 124-128

    • NAID

      40006423585

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A Relation between external electric field effect on photoluminescence and generation efficiency of electroluminescence : methylene-linked compounds of pyrene doped in a polymer film2004

    • Author(s)
      N.Ohta, T.Nakabayashi, T.Iimori, T.Yoshizawa, T.Morikawa
    • Journal Title

      Proceedings of Electroluminescence Conference 2004

      Pages: 358-361

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Time-resolved measurements of the external electric field effects on fluorescence in electron donor and acceptor pairs of N-ethylcarbazole and dimethyl terephthalate doped in a polymer film

    • Author(s)
      T.Iimori, T.Yoshizawa, T.Nakabayashi, N.Ohta
    • Journal Title

      Chem.Phys. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] External electric field effects on the photoexcitation dynamics of diphenylpolyenes

    • Author(s)
      T.Nakabayashi, M.Wahadoszamen, N.Ohta
    • Journal Title

      J.Am.Chem.Soc. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Photo-switching behavior of CdS nanoparticles doped in a polymer film

    • Author(s)
      Y.Ohara, T.Nakabayashi, K.Iwasaki, T.Torimoto, B.Ohtani, N.Ohta
    • Journal Title

      C.R.Chimie (印刷中)

    • NAID

      120000961436

    • Related Report
      2004 Annual Research Report
  • [Presentation] A Line-Scanning Multiphoton Fluorescence Spectromicroscope Applied to the Study of the Thylakoid Membrane in Cyanobacteria and Chloroplasts2007

    • Author(s)
      S. Kumazaki, et al.
    • Organizer
      14th International Congress of Photosynthesis
    • Place of Presentation
      Glasgow, Scotland
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] A Line-Scanning Semiconfocal Multiphoton Fluorescence Microscope with a Simultaneous Broadband Spectral Acquisition and its Application to the Study of the Thylakoid Membrane of a Cyanobacterium2007

    • Author(s)
      S. Kumazaki, et al.
    • Organizer
      State transition
    • Place of Presentation
      London, United Kingdom
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Micro-regulation of oxygenic photosynthesis unveiled by fluorescence microspectroscopy2007

    • Author(s)
      Shigeichi Kumazaki (Invited)
    • Organizer
      The 67th Okazaki Conference, Molecular Science and Chemical Biology of Biomolecular Function
    • Place of Presentation
      Okazaki, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Detection of quinone function in the homodimeric type-I reaction center of Heliobacterium modesticaldum2007

    • Author(s)
      T. Kondo, et al.
    • Organizer
      14^<th> International Congress of Photosynthesis
    • Place of Presentation
      Glasgow, Scotland
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Bifurcated electron donations from quinol oxidoreductase and soluble CycA to cytochrome c_z of the photosynthetic reaction center complex in the green sulfur bacterium Chlorobium2007

    • Author(s)
      C. Azai, et al.
    • Organizer
      14^<th> International Congress of Photosynthesis
    • Place of Presentation
      Glasgow, Scotland
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] The Heme-containing portion of cytochrome c_z from Chlorobium tepidum : Its over-expression in Escherichia coli and spectroscopic studies2007

    • Author(s)
      H. Oh-oka, et al.
    • Organizer
      14^<th> International Congress of Photosynthesis
    • Place of Presentation
      Glasgow, Scotland
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Compositional alternation of bacteriochlorophyll a derivatives and carotenoid species localized in RC-LH1 core complex, LH2 and LH4 of the purple bacterium, Rhodopseudomonas sp. strain Rits, under various light intensity2007

    • Author(s)
      J. Harada, et al.
    • Organizer
      Satellite Meeting : Light-harvesting Systems Workshop (LH2007)
    • Place of Presentation
      Glasgow, Scotland
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] The effect of light intensity on composition of photosynthetic pigments in light-harvesting systems of the purple photosynthetic bacterium, Rhodopseudomonas sp. strain Rits2007

    • Author(s)
      J. Harada, et a.
    • Organizer
      The 3rd International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Shiga, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Microscopic Analysis of Kinesin-microtubule Complex under High Hydrostatic Pressure2007

    • Author(s)
      M. Nishiyama, et al.
    • Organizer
      51st Annual Meeting of the Biophysical Society
    • Place of Presentation
      Baltimore, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Electric Field Effects on Fluorescence and the Relation to Dynamics in Molecular System and in Biological System2006

    • Author(s)
      N. Ohta
    • Organizer
      The Seminar at National Chiao Tung University
    • Place of Presentation
      Hsinchu, Taiwan
    • Year and Date
      2006-11-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Electric Field Effects on Photoluminescence and Photofunction of Materials2006

    • Author(s)
      N. Ohta
    • Organizer
      International Symposium on Molecular Photonics, Devoted to the memory of acad. A. N. Trenin
    • Place of Presentation
      St. Petersburg, Russia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Time-resolved Measurements of External Electric Field Effects on Fluorescence of Electron Donor-acceptor Pairs : N-ethylcarbazole and Dimethylterephthalate Doped in a Polymer Film2006

    • Author(s)
      T. Iimori, T. Yoshizawa, T. Nakabayashi, N. Ohta
    • Organizer
      XXIst IUPAC symposium on photochemistry
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] How Does High Pressure Affect on the Bacterial Motility?2006

    • Author(s)
      M. Nishiyama, et a.
    • Organizer
      5th East Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Okinawa, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Development of a High-pressure Microscope and its Applicatiion to Biological Systems2006

    • Author(s)
      M. Nishiyama, et al.
    • Organizer
      Micro-NanoMechatronics and Human Science, 2006 IEEE International Symposium
    • Place of Presentation
      Nagoya, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Development of a High-pressure Microscope and its Applicatiion to Kinesin-microtubule Complex2006

    • Author(s)
      M. Nishiyama, et al.
    • Organizer
      2006 Biophysical Discussions "Molecular Motors : Point Counterpoint"
    • Place of Presentation
      Asilomar, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Single Molecule Analysis of Stepwise Movement of ATP-driven Molecular Motors2006

    • Author(s)
      M. Nishiyama, et al.
    • Organizer
      Tamura Symposium, Frontiers in Dynamics : Physical and Biological Systems
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Methylation at the meso-position of chlorophylls by C20-methyltransferase of photosynthetic green sulfur bacteria2005

    • Author(s)
      H. Tamiaki, et al.
    • Organizer
      Pacichem2005
    • Place of Presentation
      Hawaii, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Structural basis for the methylation mechanism in methyltransferase BchU involved in bacteriochlorophyll c biosynthesis2005

    • Author(s)
      K. Wada, et al.
    • Organizer
      XX Congress of the International Union of Crystallography
    • Place of Presentation
      Florence, Italy
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] A key enzyme of biosynthesis between bacteriochlorophyll c and d, BchU, derived from the photosynthetic green sulfur bacterium Chlorobium tepidum2005

    • Author(s)
      J. Harada, et al.
    • Organizer
      Second International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Shiga, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Stepping kinetics of kinesin motors is described by load-dependent Michaelis constant2005

    • Author(s)
      M. Nishiyama, et al.
    • Organizer
      Biophysical Society 49th Annual meeting
    • Place of Presentation
      Long Beach, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Energy transfer and charge separation dynamics in chlorophyll d-based Photosystem I from Acaryochloris marina2004

    • Author(s)
      S. Kumazaki, et al.
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] The function of the PscD subunit of the photosynthetic reaction center complex in Chlorobium tepidum2004

    • Author(s)
      Y. Tsukatani, et al.
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Spectroscopic studies of electron transfer reactions in membranes and isolated reaction center from Heliobacterium modesticaldum2004

    • Author(s)
      H. Oh-oka, et al.
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Natural reverse mutations of the inactivated bchU gene in Chlorobium vibrioforme NCIB 83272004

    • Author(s)
      J. Harada, et al.
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] In Vitro Enzymatic Activity of C-20 Methyltransferase, BchU, in the Bacteriochlorophyll c Biosynthetic Pathway2004

    • Author(s)
      J. Harada, et al.
    • Organizer
      13th International Congress on Photosynthesis Satellite Workshop : Photosynthetic Light-Harvesting Systems
    • Place of Presentation
      Sainte-Adele. Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Enzymatic activity of C-20 methyltransferase, BchU, involved in biosynthesis of bacteriochlorophyll c2004

    • Author(s)
      J. Harada, et al.
    • Organizer
      International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Kusatsu, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] The electron transfer pathway in heliobacterial reaction center : Reinvestigation by a combination of the spectroscopic methods and 3-D structural modeling2004

    • Author(s)
      H. Oh-oka, et al.
    • Organizer
      International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Kusatsu, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Elucidation of stereochemistry of bacteriochlorophyll-g_F and 8^1-hydroxy-chlorophyll-a_F from Heliobacterium modesticaldum2004

    • Author(s)
      T. Mizoguchi, et al.
    • Organizer
      International Worksnop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Kusatsu, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 新実験化学講座・物質の構造I, 分光(上)2005

    • Author(s)
      太田,信廣
    • Publisher
      丸善株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 電場・磁場効果の光計測『光科学研究の最前線』2005

    • Author(s)
      太田信廣
    • Total Pages
      2
    • Publisher
      (株)国際文献印刷社
    • Related Report
      2005 Annual Research Report
  • [Book] 蛍光スペクトル『新実験化学講座・分光I』2005

    • Author(s)
      太田信廣
    • Total Pages
      24
    • Publisher
      丸善(株)
    • Related Report
      2005 Annual Research Report
  • [Book] 化学便覧 基礎編 第5版(岩澤康裕編)15.8章「電子スペクトル」2004

    • Author(s)
      太田信廣
    • Total Pages
      19
    • Publisher
      丸善
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2018-03-28  

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