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Photothermal and Photoionization Spectroscopies of Chemical Reactions in a Single Cell

Research Project

Project/Area Number 16072213
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKyushu University

Principal Investigator

HARATA Akira  Kyushu University, Faculty of Engineering Sciences, Professor (90222231)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥26,400,000 (Direct Cost: ¥26,400,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2005: ¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 2004: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsSingle Cell / Nonfluorescent Chemicals / Ultrahigh Sensitive Detection / Photothermal Spectroscopy / Microscopic Thermal Lens / Multi-color Excitation / Multiphoton Ionization / Chemical Imaging / 共鳴多光子イオン化
Research Abstract

Most of biologically important chemicals such as amino acids, nucleotides and so on, are natively nonfluorescent, and a large part of them have optical absorption only at ultraviolet wavelengths. Photothermal and photoionization spectroscopic methods have a good potential for ultrahigh sensitive in-situ detection of these chemicals. We aim to develop ultrasensitive detection and imaging methods for nonfluorescent molecules in living cells.
A photothermal signal detector under multi-color excitation has been designed for ultrasensitive detection of biological chemicals, in which transient absorption is utilized for signal enhancement. Ultraviolet excitation-visible enhancement photothermal detection system has been constructed and applied to separation analysis in combination with a micro-HPLC system. A mixture of ultraviolet-absorbing chemicals were successfully separated and detected without any labeling procedure. Two-color excitation-induced enhancement of photothermal signal was successfully observed.
An ultraviolet-excitation photothermal microscope has been developed with the excitation of the third harmonics emission of a Ti-sapphire laser and the probe laser of its fundamental emission. Photothermal images of the yeast cells were obtained. In a photothermal amplitude image of the cells, features with the size of several micrometers are seen, corresponding to the size of the cells. It was observed that a strong photothermal signal excited with the 261-nm laser beam accompanied with a small intensity of the transmitted probe light at 784-nm.
Influences of laser irradiation on a physiological function of yeast cells were quantitatively evaluated using laser light at 220, 266, 355, 532, or 1064nm. It was found that the survival probability of yeast cells after 12 hours from irradiation by 532-nm light is 10 times larger than that by 355-nm light and 104 times larger than that by 266-nm light under the same energy density condition.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (29 results)

All 2007 2006 2005 2004

All Journal Article (15 results) (of which Peer Reviewed: 2 results) Presentation (11 results) Book (3 results)

  • [Journal Article] Enhanced Photothermal Spectroscopy for Observing Chemical Reactions in Biological Cells2007

    • Author(s)
      Akira Harata
    • Journal Title

      Nao Biophotonics, H. Masuhara, S. Kawata, F. Tokunaga eds., (Elsevier BV)

      Pages: 73-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ultraviolet-Laser Excitation Microscopic Photothermal Lens Imaging for Observing Biological Cells2007

    • Author(s)
      Akira Harata, Takashi Matuda, Satoshi Hirashima
    • Journal Title

      Jpn. J. Appl. Phys. 46(7B)

      Pages: 4561-4563

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ultraviolet-Laser Excitation Microscopic Photothermal Lens Imaging for Observing Biological Cells2007

    • Author(s)
      Akira HARATA, Takashi MATUDA, Satoshi HIRASHIMA
    • Journal Title

      Japanese Journal of Applied Physics (in-press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced photothermal spectroscopy for observing chemical reactions in biological cells2007

    • Author(s)
      A.HARATA
    • Journal Title

      Nano Biophotonics Science and Technology - Handai Nanophotonics 3

      Pages: 73-92

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Growth of fluorocarbon macromolecules in the gas phase (II). The growth mechanisms of large positive ions observed in the downstream region of Ar/CF_4 plasmas2006

    • Author(s)
      Kenji FURUYA, Shinobu YUKITA, Akira HARATA
    • Journal Title

      Japanese Journal of Applied Physics 45(6A)

      Pages: 5219-5231

    • NAID

      10018149285

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 紫外レーザー励起顕微光熱変換イメージング装置の開発と細胞観察2006

    • Author(s)
      原田 明, 松田 貴志, 平島 諭
    • Journal Title

      電子情報通信学会技術研究報告 106(294)

      Pages: 1-4

    • NAID

      110004851818

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of Alkyl Chain Length on Photoionization at the Water Surface2006

    • Author(s)
      Toshio ISHIOKA, Akira HARATA
    • Journal Title

      UVSOR Activity Report 2005

      Pages: 70-70

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Detection of Host-guest Complexation at the Water Surface by Photoionization Measurements2005

    • Author(s)
      Toshio ISHIOKA, Akira HARATA
    • Journal Title

      UVSOR Activity Report 2004

      Pages: 71-71

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Acidity Effects on the Adsorptive Behavior and Fluorescence Properties of Rhodamine 6G Molecules at the Air-Water Interface Studied with Confocal Fluorescence Microscopy2004

    • Author(s)
      Xue-Ying Zhengt, Mika Wachi, Akira Harata, Yoshihiko Hatano
    • Journal Title

      Specrtochimica Acta Part A 60

      Pages: 1085-1090

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Acid-Base Equilibrium Constants and Distributio Coefficients of Aminopyrene between the Surface and Bulk of Liquid Water as Studied by a Laser Two-Photon Ionization Technique2004

    • Author(s)
      Miki Sato, Akira Harata, Yoshihiko Hatano, Teiichiro Ogawa, Takeshi Kaieda
    • Journal Title

      Journal of Physical Chemistry 108

      Pages: 12111-12115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Acidity Effects on the Adsorptive Behavior and Fluorescence Properties of Rhodamine 6G Molecules at the Air-Water Interface Studied with Confocal Fluorescence Microscopy2004

    • Author(s)
      Xue-Ying Zhengt, Mika Wachi, Akira Harata, Yoshihiko Hatano
    • Journal Title

      Specrtochim. Acta Part A Vol. 60, No. 5

      Pages: 1085-1090

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acid-Base Equilibrium Constants and Distribution Coefficients of Aminopyrene between the Surface and Bulk of Liquid Water as Studied by a Laser Two-Photon Ionization Technique2004

    • Author(s)
      Miki Sato, Akira Harata, Yoshihiko Hatano, Teiichiro Ogawa, Takeshi Kaieda
    • Journal Title

      J. Phys. Chem. B Vol. 108, No. 32

      Pages: 12111-12115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation and Photosensitivity to Femtosecond Laser Pulses of Samarium Sulfide Thin Films2004

    • Author(s)
      Ryota Kitagawa, *Hiromichi Takebe, Akira Harata, Kenji Morinaga
    • Journal Title

      J. Ceram. Soc. Jpn. Vol. 112, No. 5-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acidity Effects on the Adsorptive Behavior and Fluorescence Properties of Rhodamine 6G Molecules at the Air-Water Interface Studied with Confocal Fluorescence Microscopy2004

    • Author(s)
      Xue-Ying ZHENG, Mika WACHI, Akira HARATA, Yoshihiko HATANO
    • Journal Title

      Specrtochimica Acta Part A 60・5

      Pages: 1085-1090

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Acid-Base Equilibrium Constants and Distribution Coefficients of Aminopyrene between the Surface and Bulk of Liquid Water as Studied by a Laser Two-Photon Ionization Technique2004

    • Author(s)
      Miki SATO, Akira HARATA, Yoshihiko HATANO, Teiichiro OGAWA, Takeshi KAIEDA
    • Journal Title

      Journal of Physical Chemistry B 108・32

      Pages: 12111-12115

    • Related Report
      2004 Annual Research Report
  • [Presentation] Ultraviolet-Laser Excitation Microscopic Photothermal Lens Measurements for Observing Chemical Compounds in Living Cells2006

    • Author(s)
      Akira Harata
    • Organizer
      3rd International Nanophotonics Symposium Handai
    • Place of Presentation
      Suita, Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Ultraviolet-Laser Excitation Microscopic Photothermal Lens Imaging for Observing Biological Cells2006

    • Author(s)
      Akira Harata, Takashi Matuda, Satoshi Hirashima
    • Organizer
      28th Symposium on Ultrasonic Electronics
    • Place of Presentation
      Nagoya, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Development of an Evaluation Method for Wavelength-Dependent Photo-Induced Damage of Living Cells2006

    • Author(s)
      K. Sassa, S. Hirashima, J. Kashiwagi, M. Isoda, A. Harata
    • Organizer
      8th Cross Straits Symposium on Materials, Energy and Environmental Sciences (CSS8)
    • Place of Presentation
      Pusan, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Design of a photothermal lens amplification system for ultrasensitive detection of nonfluorescent chemicals2005

    • Author(s)
      Satoshi Hirashima, Akira Harata
    • Organizer
      2005 International Chemical Congress of Pacific Basin Societies (Pacifichem 2005)
    • Place of Presentation
      Honolulu, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Analysis of environmental pollutant at the air/water interface by using photoionization methods2005

    • Author(s)
      Yuki Maeda, Miki Isoda, Toshio Ishioka, Akira Harata
    • Organizer
      2005 International Chemical Congress of Pacific Basin Societies (Pacifichem 2005)
    • Place of Presentation
      Honolulu, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Development of a transient absorption spectrometer for environmental substances and biomaterials2005

    • Author(s)
      Takashi Matsuda, Akira Harata
    • Organizer
      2005 International Chemical Congress of Pacific Basin Societies (Pacifichem 2005)
    • Place of Presentation
      Honolulu, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Analysis of photoinduced current at liquid/liquid interface2005

    • Author(s)
      Nobuhiro Inoue, Toshio Ishioka, Akira Harata
    • Organizer
      2005 International Chemical Congress of Pacific Basin Societies (Pacifichem 2005)
    • Place of Presentation
      Honolulu, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Design of a microscopic transient absorption spectrometer for environmental substances and biomaterials2005

    • Author(s)
      T. Matsuda, A. Harata
    • Organizer
      7th Cross Straits Symposium on Materials, Energy and Environmental Sciences
    • Place of Presentation
      Fukuoka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Analysis of Environmental Chloride and nitride pollutants at the water surface by photoionizationb methods using laser and synchrotron radiation2005

    • Author(s)
      Y. Maeda, M. Isoda, T. Ishioka, A. Harata
    • Organizer
      7th Cross Straits Symposium on Materials, Energy and Environmental Sciences
    • Place of Presentation
      Fukuoka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Effects of molecular adsorption on photo induced current at water/hexane interface2005

    • Author(s)
      N. Inoue, T. Ishioka, A. Harata
    • Organizer
      7th Cross Straits Symposium on Materials, Energy and Environmental Sciences
    • Place of Presentation
      Fukuoka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Amplification of Photothermal Lens Signal by Two-Color Crossed-Beam Excitation2004

    • Author(s)
      S. Hirashima, A. Harata
    • Organizer
      6th Cross Straits Symposium on Materials, Energy and Environmental Engineering
    • Place of Presentation
      Pohang Univ. Science and Technology, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Nao Biophotonics (分担執筆 : Enhanced Photothermal Spectroscopy for Observing Chemical Reactions in Biological Cells)2007

    • Author(s)
      Akira Harata
    • Total Pages
      428
    • Publisher
      Elsevier BV
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 先端化学シリーズVI 界面・コロイド ナノテクノロジー 分子エレクトロニクス ナノ分析化学(日本科学会編)2004

    • Author(s)
      原田 明 (分担執筆)
    • Total Pages
      305
    • Publisher
      丸善
    • Related Report
      2004 Annual Research Report
  • [Book] 先端の分析法-理工学からナノ・バイオまで-2004

    • Author(s)
      原田 明 (分担執筆)
    • Total Pages
      978
    • Publisher
      NTS
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2018-03-28  

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