Regulation mechanism of the intracellular transport by kinesin

• Project/Area Number: 16083204
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: TOMISHIGE Michio The University of Tokyo, 大学院・工学系研究科, 准教授 (50361530)
• Co-Investigator(Kenkyū-buntansha): NISHIZAKA Takayuki 学習院大学, 理学部, 教授 (40359112)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥72,600,000 (Direct Cost: ¥72,600,000); Fiscal Year 2008: ¥5,400,000 (Direct Cost: ¥5,400,000); Fiscal Year 2007: ¥6,600,000 (Direct Cost: ¥6,600,000); Fiscal Year 2006: ¥19,000,000 (Direct Cost: ¥19,000,000); Fiscal Year 2005: ¥20,900,000 (Direct Cost: ¥20,900,000); Fiscal Year 2004: ¥20,700,000 (Direct Cost: ¥20,700,000)
• Keywords: 分子モーター / 一分子計測 / 蛍光観察 / 蛍光共鳴エネルギー移動 / 構造変化 / 細胞内輸送

Research Abstract

運動中の分子モーターキネシンの構造変化を、蛍光共鳴エネルギー移動法を用いて一分子レベルで検出する方法を開発した。この方法を用いてキネシンの協調的二足歩行運動を引き起こす構造的基盤を明らかにした。また2種類の分子モーターを混合した微小管滑り運動系や2分子のキネシンの特異的架橋のアッセイ系を確立し、これらを用いて複数分子モーター間の協調性を定量的に解析した。

Research Products

• [Journal Article] Intramolecular strain coordinates kinesin stepping behavior along microtubules (2008)
  • Author(s): A. Yildiz, M. Tomishige, A. Gennerich, and R. D. Vale
  • Journal Title: Cell 134 Pages: 1030-1041
  • Peer Reviewed
• [Journal Article] A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking (2008)
  • Author(s): J. Yajima, K. Mizutani, T. Nishizaka
  • Journal Title: Nat. Struct. Mol. Biol. 15 Pages: 1119-1121
  • Peer Reviewed
• [Journal Article] Activation of mitotic kinesin by microtubule bundling (2008)
  • Author(s): M. Tomishige
  • Journal Title: J. Cell Biol. 182 Pages: 417-419
• [Journal Article] 細胞内輸送を担う分子モーターの運動機構 (2008)
  • Author(s): 富重道雄
  • Journal Title: 生体の科学 59 Pages: 362-363
• [Journal Article] Intramolecular strain coordinates kinesin stepping behavior along microtubules (2008)
  • Author(s): A. Yildiz
  • Journal Title: Cell 134 Pages: 1030-1041
  • Peer Reviewed
• [Journal Article] A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking (2008)
  • Author(s): J. Yajima
  • Journal Title: Nat. Struct. Mol. Biol 15 Pages: 1119-1121
  • Peer Reviewed
• [Journal Article] Activation of mitotic kinesin by microtubule bundling (2008)
  • Author(s): M. Tomishige
  • Journal Title: J. Cell Biol 182 Pages: 417-419
• [Journal Article] How kinesin waits between steps (2007)
  • Author(s): T. Mori, R. D. Vale and M. Tomishige
  • Journal Title: Nature 450 Pages: 750-754
  • Peer Reviewed
• [Journal Article] Single-molecule observation of rotation of F_1-ATPase through micro beads (2007)
  • Author(s): T. Nishizaka, K. Mizutani and T. Masaike
  • Journal Title: Methods Mol. Biol. 392 Pages: 171-181
• [Journal Article] How kinesin waits between steps. (2007)
  • Author(s): T. Mori
  • Journal Title: Nature 450 Pages: 750-754
  • Peer Reviewed
• [Journal Article] Single-molecule observations of neck linker conformational changes in the kinesin motor protein (2006)
  • Author(s): M. Tomishige, N. Stuurman and R. D. Vale
  • Journal Title: Nat. Struct. Mol. Biol. 13 Pages: 887-894
  • Peer Reviewed
• [Journal Article] 一分子計測法によって明らかになったモータータンパク質キネシンの二足歩行の仕組み (2006)
  • Author(s): 富重道雄
  • Journal Title: 物性研究 85 Pages: 624-629
• [Journal Article] Single molecule observations of the neck linker conformational changes in the kinesin motor protein. (2006)
  • Author(s): Michio Tomishige, Nico Stuurman, Ronald D. Vale
  • Journal Title: Nature Structural and Molecular Biology 13・10 Pages: 887-894
• [Journal Article] 一分子計測法により明らかになったモータータンパク質キネシンの二足歩行の仕組み (2006)
  • Author(s): 富重道雄
  • Journal Title: 物性研究 85 Pages: 624-629
• [Journal Article] 二本足で歩くタンパク質キネシン (2005)
  • Author(s): 富重道雄
  • Journal Title: Bionics 2 Pages: 64-70
• [Journal Article] Kinesin walks hand-over-hand (2004)
  • Author(s): A. Yildiz, M. Tomishige, R. D. Vale and P. R. Selvin
  • Journal Title: Science 303 Pages: 676-678
• [Journal Article] Chemomechanical coupling in F1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation (2004)
  • Author(s): T. Nishizaka, K. Oiwa, H. Noji, S. Kimura, E. Muneyuki, M. Yoshida and K. Kinosita Jr.
  • Journal Title: Nat. Struct. Mol. Biol. 11 Pages: 142-148
• [Journal Article] Kinesin walks hand-over-hand (2004)
  • Author(s): Yildiz, A., Tomishige, M., Vale, R.D., P.R.Selvin
  • Journal Title: Science 303 Pages: 676-678
• [Journal Article] Chemomechanical coupling in F1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation (2004)
  • Author(s): Nishizaka, T., Oiwa, K., Noji, H., Kimura, S., Muneyuki, E., et al.
  • Journal Title: Nature Structural and Molecular Biology 11 Pages: 142-148
• [Presentation] Processive motility of heterodimeric kinesin that has defect in the neck linker docking (2009)
  • Author(s): T. Mori, M. Nakajima and M. Tomishige
  • Organizer: 53rd Annual Meeting of Biophysical Society
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2009-03-01
• [Presentation] Processive motility of heterodimeric kinesin that has defect in the in the neck linker docking (2009)
  • Author(s): Teppei Mori, Michiko Nakajima, and Michio Tomishige
  • Organizer: 53rd Annual Meeting of Biophysical Society
  • Place of Presentation: Boston, MA
  • Year and Date: 2009-03-01
• [Presentation] キネシンの協調的な二足歩行運動に対するネックリンカーの役割 (2008)
  • Author(s): 磯島広, 豊北祐士, 森徹平, 富重道雄
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] キネシン2量体の前後の頭部へのATP結合の可視化 (2008)
  • Author(s): 凌審、森徹平、中島理子、富重道雄
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] 単頭と双頭のキネシン分子モーターによる協調的運動 (2008)
  • Author(s): 尾世川麻里奈, 小橋川翔太, 森徹平, 富重道雄
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-03
• [Presentation] Single molecule observation of conformational changes of kinesin (2008)
  • Author(s): M. Tomishige
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: The University of Tokyo
  • Year and Date: 2008-11-02
• [Presentation] Single molecule observation of conformational changes of kinesin (2008)
  • Author(s): Michio Tomishige
  • Organizer: 2^<nd> International Symposium on Bio-nanosystems
  • Place of Presentation: Koshiba Hall, The University of Tokyo
  • Year and Date: 2008-11-02
• [Presentation] 微小管上を歩く分子モーターキネシンの運動の仕組み (2008)
  • Author(s): 富重道雄
  • Organizer: 第30回北海道大学獣医学学術交流基金群講演会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-10-15
• [Presentation] 微小管上を歩く分子モーターキネシンの運動の仕組み (2008)
  • Author(s): 富重道雄
  • Organizer: 平成20年度(第30回)北海道大学獣医学学術交流基金群講演会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-10-15
• [Presentation] Single molecule FRET observations of nucleotide-dependent configuration changes of kinesin dimer (2008)
  • Author(s): T. Mori, R. D. Vale and M. Tomishige
  • Organizer: Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysical Congress
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 2008-02-05
• [Presentation] 二足歩行する分子モーターキネシンから見えてきたエネルギー変換機構 (2007)
  • Author(s): 富重道雄
  • Organizer: 日本生物物理学会第45回年会シンポジウム
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2007-12-23
• [Presentation] Single molecule structural observations of the molecular motor kinesin (2007)
  • Author(s): M. Tomishige
  • Organizer: COE International Symposium on "Strongly-Correlated Applied Physics"
  • Place of Presentation: 東京大学武田ホール
  • Year and Date: 2007-09-29
• [Presentation] Conformation of kinesin dimer at ATP-waiting state probed by single molecule FRET (2007)
  • Author(s): T. Mori, R. D. Vale and M. Tomishige
  • Organizer: 51st Annual Meeting of Biophysical Society
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 2007-03-06
• [Presentation] Single molecule FRET observations of structural changes in the neck linker of kinesin (2007)
  • Author(s): M. Tomishige, N. Stuurman and R. D. Vale
  • Organizer: 51st Annual Meeting of Biophysical Society
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 2007-03-05
• [Presentation] Single molecule observations of structural changes in a "walking" motor protein (2006)
  • Author(s): M. Tomishige
  • Organizer: Ninth Annual Japanese-American Kavli Frontiers of Science Symposium
  • Place of Presentation: Beckman Center, Irvine, CA, USA
  • Year and Date: 2006-12-09
• [Presentation] Observation of the tug of war movement of microtubules by kinesin and dynein molecules in vitro (2006)
  • Author(s): H. Tsurusawa, H. Tadakuma, T. Shima, R. Ohkura, T. Kon, K. Sutoh and M. Tomishige
  • Organizer: Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Okinawa Convention Center, Okinawa, Japan
  • Year and Date: 2006-11-15
• [Presentation] 逆向きに運動するモーター蛋白質による微小管の綱引き運動の蛍光顕微鏡観察 (2005)
  • Author(s): 藤原弘章, 多田隈尚史, 富重道雄
  • Organizer: 第43回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-23
• [Presentation] Single molecule observation of structural changes of kinesin (2005)
  • Author(s): M. Tomishige
  • Organizer: 61st Harden Conference - Molecular Motors Structure and Function
  • Place of Presentation: Cambridge, UK
  • Year and Date: 2005-09-19