Regulation of information flow by motor protein systems
Project/Area Number |
16083205
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | The University of Tokyo |
Principal Investigator |
SUTOH Kazuo The University of Tokyo, 大学院・総合文化研究科, 教授 (20111453)
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Co-Investigator(Kenkyū-buntansha) |
昆 隆英 東京大学, 大学院・総合文化研究科, 助教 (30332620)
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Co-Investigator(Renkei-kenkyūsha) |
KON Takahide 東京大学, 大学院・総合文化研究科, 助教 (30332620)
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Project Period (FY) |
2004 – 2008
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Project Status |
Completed (Fiscal Year 2008)
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Budget Amount *help |
¥64,800,000 (Direct Cost: ¥64,800,000)
Fiscal Year 2008: ¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 2007: ¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2006: ¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2005: ¥21,600,000 (Direct Cost: ¥21,600,000)
Fiscal Year 2004: ¥8,100,000 (Direct Cost: ¥8,100,000)
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Keywords | モーターたんぱく質 / ミオシン / ダイニン / AAA+ATPase / FRET / モータータンパク質 / ヘテロダイマータグ / 部位特異的蛍光標識 / 微小管 / レバーアーム仮説 / 1分子計測 / REFT / 全反射顕微鏡 / AAA+タンパク質 / X線結晶解析 / ミオシンスーパーファミリー / ハイスループットスクリーニング / タンパク質複合体 / 膜貫通型セリン / スレオニンキナーゼ / プロテアソーム / 細胞内輸送 / ミオシン6 / ミオシン18 / チロシンキナーゼ / 酵母ツーハイブリッド / エンドサイトーシス |
Research Abstract |
Our major goal of this project is to elucidate structures and functions of motor proteins, especially, unconventional myosin 6 and cytoplasmic dynein. We carried out the yeast two-hybrid screening of interacting partners of myosin 6. We identified several proteins including a membrane-bound tyrosine kinase. We examined in vivo functions of the kinase by using various cell- and molecular-biological techniques such as RNAi. We also examined structure and function of cytoplasmic dynein, using recombinant dynein motor domain. We identified interesting biochemical and structural properties of the motor domain by electron microscopy, rapid kinetics and FRET (fluorescence resonance energy transfer). The data obtained by these experiments will become the basis for understanding cellular functions of motor proteins.
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Report
(6 results)
Research Products
(48 results)
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[Journal Article] AAA+ Ring and Linker Swing Mechanism in the Dynein Motor2009
Author(s)
Anthony J. Roberts, Naoki Numata, Matt L. Walker, Yusuke S. Kato, Bara Malkova, Takahide Kon, Reiko Ohkura, Fumio Arisaka, Peter J. Knight, Kazuo Sutoh, Stan A. Burgess
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[Journal Article] Autonomous Loading/Unloading and Transport of Specified Cargoes Using DNA Hybridization and Biological Motor-Based Motility2008
Author(s)
Hiyama, S., Inoue, T., Shima, T., Moritani, Y., Suda, T., Sutoh, K
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[Journal Article] A single-headed recombinant fragment of Dictyostelium cytoplasmic dynein can drive the robust sliding of microtubules2004
Author(s)
Nishiura, M., Kon, T., Shiroguchi, K., Ohkura, R., Shima, T., Toyoshima, Y.Y., Sutoh, K
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Journal Title
J. Biol. Chem 279
Pages: 22799-22802
Related Report
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[Journal Article] Distinct functions of nucleotide-binding/hydrolysis sites in the four AAA modules of cytoplasmic dynein, as revealed by biochemical characterizations of recombinant fragments.2004
Author(s)
Kon, T., Nishiura, M., Ohkura, R., Toyoshima, Y.Y., Sutoh, K.
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Journal Title
Biochemistry 43
Pages: 11266-11274
Related Report
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[Journal Article] A single-headed recombinant fragment of Dictyostelium cytoplasmic dynein can drive the robust sliding of microtubules.2004
Author(s)
Nishiura, M., Kon, T., Shiroguchi, K., Ohkura, R., Shima, T., Toyoshima, Y.Y., Sutoh, K.
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Journal Title
J.Biol.Chem. 279
Pages: 22799-22802
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