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Integration of intracellular nanosystems in calcium signaling

Research Project

Project/Area Number 16083206
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University (2005-2008)
The University of Tokyo (2004)

Principal Investigator

HIROSE Kenzo  Nagoya University, 大学院・医学系研究科, 教授 (00292730)

Research Collaborator NAMIKI Shigeyuki  名古屋大学, 大学院・医学系研究科, 助教 (90452193)
OHTA Yusaku  
KAMIYA Mako  名古屋大学, 大学院・医学系研究科, 日本学術振興会特別研究員SPD
HIRANO Tomohiro  名古屋大学, 大学院・医学系研究科, 研究員
SUGAO Kohtaro  東京大学, 大学院・医学系研究科, 博士課程学生
IINUMA Sho  東京大学, 大学院・医学系研究科, 博士課程学生
TAKEI Daisuke  名古屋大学, 大学院・医学系研究科, 博士課程学生
SAKAMOTO Hirokazu  名古屋大学, 大学院・医学系研究科, 博士課程学生
ISA Masayuki  名古屋大学, 大学院・医学系研究科, 修士課程学生
TAIKO Isamu  名古屋大学, 大学院・医学系研究科, 修士課程学生
TAKIKAWA Kenji  名古屋大学, 大学院・医学系研究科, 修士課程学生
KISHI Shunsuke  名古屋大学, 大学院・医学系研究科, 修士課程学生
KANDA Mai  名古屋大学, 大学院・医学系研究科, 修士課程学生
TADOKORO Dai  名古屋大学, 大学院・医学系研究科, 博士課程学生
Project Period (FY) 2004 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥52,000,000 (Direct Cost: ¥52,000,000)
Fiscal Year 2008: ¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 2007: ¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2006: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2005: ¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2004: ¥8,700,000 (Direct Cost: ¥8,700,000)
Keywordsカルシウム / 細胞・組織 / シグナル伝達 / 生体分子 / ナノシステム
Research Abstract

本研究では、カルシウムシグナリングに焦点を当て、生体を構成する分子からなる細胞内ナノシステムがどのように細胞機能を実現するかを解明するため、新しい分子イメージングプローブの開発を行った。開発されたプローブによって、分子の可視化を行うことに成功し、カルシウムシグナルがどのように制御されているかについてその実態を明らかにした。

Report

(6 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (33 results)

All 2009 2008 2007 2006 2005 2004 Other

All Journal Article (22 results) (of which Peer Reviewed: 13 results) Presentation (7 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Selective photoinactivation of protein function through environment-sensitive switching of singlet oxygen generation by photosensitizer2008

    • Author(s)
      Yogo T, Urano Y, Mizushima A, Sunahara H, Inoue T, Hirose K, Iino M, Kikuchi K, Nagano T.
    • Journal Title

      PNAS 105

      Pages: 28-32

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective photoinactivation of protein function through environment-sensitive switching of singlet oxygen generation by photosensitizer.2008

    • Author(s)
      余郷, ら
    • Journal Title

      Proc Natl Acad Sci U S A. 105

      Pages: 28-32

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of neurite growth by spontaneous Ca2+ oscillations in astrocytes2007

    • Author(s)
      Kanemaru K, Okubo Y, Hirose K, Iino M.
    • Journal Title

      J Neurosci 27

      Pages: 8957-66

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of neurite growth by spontaneous Ca2+ oscillations in astrocytes.2007

    • Author(s)
      金丸, ら
    • Journal Title

      J Neurosci. 27

      Pages: 8957-8966

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+ lightning conveys cell-cell contact information inside the cells2006

    • Author(s)
      Hashido, M., Hayashi, K., Hirose, K., Iino, M.
    • Journal Title

      EMBO Rep. 7

      Pages: 1117-1123

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+ shuttling between endoplasmic reticulum and mitochondria underlying Ca2+ oscillations2006

    • Author(s)
      Ishii, K., Hirose, K., Iino, M.
    • Journal Title

      EMBO Rep. 7

      Pages: 390-396

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+ lightning conveys cell-cell contact information inside the cells.2006

    • Author(s)
      Hashido M, Hayashi K, Hirose K, Iino M
    • Journal Title

      EMBO Report 7

      Pages: 1117-1123

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ca2+ shuttling between endoplasmic reticulum and mitochondria underlying Ca2+ oscillations2006

    • Author(s)
      Ishii K, Hirose K, Iino M.
    • Journal Title

      EMBO Report

    • Related Report
      2005 Annual Research Report
  • [Journal Article] NO signalling decodes frequency of neuronal activity and generates synapse-specific plasticity in mouse cerebellum2005

    • Author(s)
      Namiki S, Kakizawa S, Hirose K, Iino M.
    • Journal Title

      J Physiol 566

      Pages: 849-863

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] BAD detects coincidence of G2/M phase and growth factor deprivation to regulate apoptosis2005

    • Author(s)
      Hashimoto A, Hirose K, Iino M.
    • Journal Title

      J Biol Chem. 280

      Pages: 26225-26232

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time imaging of myosin II regulatory light chain phosphorylation using a new protein biosensor2005

    • Author(s)
      Yamada, A., Hirose, K., Hashimoto, A., Iino, M.
    • Journal Title

      Biochem. J. 385

      Pages: 589-594

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of fluorescein as a platform for finely tunable fluorescence probes2005

    • Author(s)
      Urano, Y., Kamiya, M., Kanda, K., Ueno, T., Hirose, K., Nagano, T.
    • Journal Title

      J Am Chem Soc. 127

      Pages: 4888-4894

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] NO signalling decodes frequency of neuronal activity and generates synapse-specific plasticity in mouse cerebellum2005

    • Author(s)
      Shigeyuki Namiki, Sho Kakizawa, Kenzo Hirose, Masamitsu Iino
    • Journal Title

      The Journal of Physiology 566

      Pages: 849-863

    • Related Report
      2005 Annual Research Report
  • [Journal Article] BAD detects coincidence of G2/M phase and growth factor deprivation to regulate apoptosis.2005

    • Author(s)
      Hashimoto A, Hirose K, Iino M.
    • Journal Title

      J Biol Chem. 280

      Pages: 26225-26232

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Real-time imaging of myosin II regulatory light chain phosphorylation using a new protein biosensor.2005

    • Author(s)
      Yamada, A., Hirose, K., Hashimoto, A., Iino, M.
    • Journal Title

      Biochem.J. 385

      Pages: 589-594

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Evolution of Fluorescein as a Platform for Finely Tunable Fluorescence Probes2005

    • Author(s)
      Yasuteru Urano, Mako Kamiya, Kojiro Kanda, Tasuku Ueno, Kenzo Hirose, Tetsuo Nagano
    • Journal Title

      J.Am.Chem.Soc. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cross talk between metabotropic and ionotropic glutamate receptor-mediated signaling in parallel fiber-induced inositol 1, 4, 5-trisphosphate production in cerebellar Purkinje cells2004

    • Author(s)
      Okubo, Y., Kakizawa, S., Hirose, K., Iino, M.
    • Journal Title

      J Neurosci 24

      Pages: 9513-9520

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modification of intracellular Ca2+ dynamics by laser inactivation of inositol 1, 4, 5-triphosphate receptor using membrane-permeant probes2004

    • Author(s)
      Yogo, T., Kikuchi, K., Inoue, T., Hirose, K., Iino, M., Nagano, T.
    • Journal Title

      Chemistry & Biology 11

      Pages: 1053-1058

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photo-acceleration of protein release from endosome in the protein transduction system2004

    • Author(s)
      Matsushita M, Noguchi H, Lu YF, Tomizawa K, Michiue H, Li ST, Hirose K, Bonner-Weir S, Matsui H.
    • Journal Title

      FEBS Lett. 572

      Pages: 221-226

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modification of intracellular Ca2+ dynamics by laser inactivation of inositol 1,4,5-trisphosphate receptor using membrane-permeant probes.2004

    • Author(s)
      Yogo T, Kikuchi K, Inoue T, Hirose K, _Iino M, Nagano T.
    • Journal Title

      Chem.Biol. 11

      Pages: 1053-1058

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cross talk between metabotropic and ionotropic glutamate receptor-mediated signaling in parallel fiber-induced inositol 1,4,5-trisphosphate production in cerebellar Purkinje cells.2004

    • Author(s)
      Okubo Y, Kakizawa S, Hirose K, Iino M.
    • Journal Title

      J.Neurosci. 24

      Pages: 9513-9520

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Photo-acceleration of protein release from endosome in the protein transduction system.2004

    • Author(s)
      Matsushita M, Noguchi H, Lu YF, Tomizawa K, Michiue H, Li ST, Hirose K, Bonner-Weir S, Matsui H.
    • Journal Title

      FEBS Lett. 572

      Pages: 221-226

    • Related Report
      2004 Annual Research Report
  • [Presentation] ゲノムワイドRNAiライブラリー構築技術の開発2009

    • Author(s)
      太向勇, ら
    • Organizer
      日本薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-03-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] サブタイプ特異的受容体型チロシンキナーゼシグナリングの人工的制御2009

    • Author(s)
      伊佐真幸, ら
    • Organizer
      日本薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-03-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生細胞分子イメージングのためのハイブリッド型プローブ2008

    • Author(s)
      廣瀬謙造
    • Organizer
      日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2008-06-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細胞内カルシウムシグナリングの可視化2006

    • Author(s)
      廣瀬謙造
    • Organizer
      第79回日本薬理学会年会学術奨励賞受賞講演
    • Place of Presentation
      横浜
    • Year and Date
      2006-03-09
    • Related Report
      2008 Final Research Report
  • [Presentation] EPRILテクノロジーによるRNAiライブラリー2006

    • Author(s)
      廣瀬謙造
    • Organizer
      第79回日本薬理学会年会シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2006-03-08
    • Related Report
      2008 Final Research Report
  • [Presentation] RNAi library for Potential Dug Target discovery2006

    • Author(s)
      Kenzo Hirose.
    • Organizer
      XVth World Congress of Pharmacology IUPHAR
    • Place of Presentation
      北京
    • Related Report
      2008 Final Research Report
  • [Presentation] 小脳プルキンエ細胞におけるNOイメージング2005

    • Author(s)
      廣瀬謙造
    • Organizer
      日本顕微鏡学会第61回学術講演会
    • Place of Presentation
      筑波
    • Year and Date
      2005-06-01
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.cir.tohoku.ac.jp/higuchi-p/NanoSystem/index.htm

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.med.nagoya-u.ac.jp/Seiri1/

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.neurobiol.m.u-tokyo.ac.jp/

    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] rnaiライブラリーの酵素的構築方法2004

    • Inventor(s)
      廣瀬謙造外4名
    • Industrial Property Rights Holder
      株式会社東京大学tlo
    • Filing Date
      2004-12-28
    • Related Report
      2008 Final Research Report

URL: 

Published: 2004-04-01   Modified: 2018-03-28  

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