Molecular mechnisms of dynein's force generation and regulations

• Project/Area Number: 16083207
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: OIWA Kazuhiro National Institute of Information and Communications Technology, 未来ICT研究センター, センター長 (10211096)
• Co-Investigator(Renkei-kenkyūsha): IWAMOTO Hiroyuki 財団法人高緯度光科学研究センター, 利用研究促進準部門, 主幹研究員 (60176568)
• Research Collaborator: BURGESS Stan A. University of Leeds, Inst. Mol. Cellular Biol., Lecturer ; ISIKAWA Takashi ETH Zurich, Inst. Mol. Biol. Biophys., Groop Leader
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥62,500,000 (Direct Cost: ¥62,500,000); Fiscal Year 2008: ¥7,300,000 (Direct Cost: ¥7,300,000); Fiscal Year 2007: ¥10,400,000 (Direct Cost: ¥10,400,000); Fiscal Year 2006: ¥16,200,000 (Direct Cost: ¥16,200,000); Fiscal Year 2005: ¥20,600,000 (Direct Cost: ¥20,600,000); Fiscal Year 2004: ¥8,000,000 (Direct Cost: ¥8,000,000)
• Keywords: 一分子計測(SMD) / 生物物理 / ナノマシン / ナノバイオ / 分子モーター

Research Abstract

本研究課題では、タンパク質モータ、軸糸ダイニンについて単一分子計測やアンサンブル機能計測、電子顕微鏡やX線溶液散乱による構造解析など、その構造と機能に関する研究を統一的に実施、ダイニンの運動発生機構とその制御機構の解明を目指した。生理的溶液中では伸展した構造をとるダイニンは、軸糸内においてコンパクトな構造となり隣接するダイニン分子の間に構造的連関を生じることを明らかにした。これは、個々の機能がアンサンブルとして高い次元の機能へと組織化して統合された鞭毛波を作り出すための構造的基盤である。機能面では運動特性の大きく異なる2種類のダイニンの協調的運動特性を明らかにして、鞭毛軸糸内でのアンサンブル特性創発の機能的基礎の一端を明らかにした。

Research Products

• [Journal Article] Single Molecule Biology (ed. A. E. Knight) (2009)
  • Author(s): Kazuhiro Oiwa
  • Pages: 61-104
• [Journal Article] Cooperative three-step motions in catalytic subunits of F-1-ATPase correlate with 80 degress and 40 degrees substep rotations. (2008)
  • Author(s): Masaike, T., Koyama-Horibe, F., Oiwa K., Yoshida, M. & Nishizaka, T.
  • Journal Title: Nature Struct. Mol. Biol. 15 Pages: 1326-1333
  • Peer Reviewed
• [Journal Article] Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella. (2008)
  • Author(s): Bui, K. H., Sakakibara, H., Movassagh, T., Oiwa, K. & Ishikawa, T.
  • Journal Title: J. Cell Biol. 183 Pages: 923-932
  • Peer Reviewed
• [Journal Article] Molecular motors : Keep on moving (2008)
  • Author(s): Oiwa, K. and Kojima, H.
  • Journal Title: Nature Nanotech. 3 Pages: 531-532
• [Journal Article] Cooperative three-step motions in catalytic subunits of F1-ATPase correlate with 80°and 40°substep rotations (2008)
  • Author(s): Tomoko Masaike
  • Journal Title: Nature Structural and Molecular Biology 15 Pages: 1326-1333
  • Peer Reviewed
• [Journal Article] Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii fl agella (2008)
  • Author(s): Khanh Huy Bui
  • Journal Title: Journal of Cell Biology 183 Pages: 923-932
  • Peer Reviewed
• [Journal Article] Molecular Motors : Keep on moving (2008)
  • Author(s): Kazuhiro Oiwa
  • Journal Title: Nature Nanotechnology 3 Pages: 531-532
• [Journal Article] Mechanical properties of inner-arm dynein-f (dynein I1) studied with in vitro motility assays. (2007)
  • Author(s): Kotani, N., Sakakibara, H., Burgess, S. A., Kojima, H., and Oiwa, K.
  • Journal Title: Biophys. J. 93 Pages: 886-894
  • Peer Reviewed
• [Journal Article] Coupling of rotation and catalysis in F1-ATPase revealed by single-molecule imaging and manipulation (2007)
  • Author(s): Adachi, K., Oiwa, K., Nishizaka, T., Furuike, K., Noji, K., Itoh, H., Yoshida, M., and Kinosita, K. Jr.
  • Journal Title: Cell 130 Pages: 309-321
  • Peer Reviewed
• [Journal Article] The architectue of outer dynein arms in situ (2007)
  • Author(s): Ishikawa, T. Sakakibara, H., and Oiwa, K.
  • Journal Title: J. Mol. Biol. 368 Pages: 1249-1258
  • Peer Reviewed
• [Journal Article] Coupling of rotation and catalysis in F1-ATPase revealed by single-molecule imaging and manipulation. (2007)
  • Author(s): Adachi, K.
  • Journal Title: Cell 130 Pages: 309-321
  • Peer Reviewed
• [Journal Article] Mechanical properties of inner-arm dynein-f(dyneinI1)studied with in vitromotility assays. (2007)
  • Author(s): Kotani, N.
  • Journal Title: Biophysica Journal 93 Pages: 886-894
  • Peer Reviewed
• [Journal Article] The coordination of protein motors and the kinetic behavio of microtubule-acomputational study. (2007)
  • Author(s): Chen, Q.
  • Journal Title: Biophysical Chemistry 129 Pages: 60-69
  • Peer Reviewed
• [Journal Article] Diversity of structural behavior in vertebrate conventional myosins complexed with actin. (2007)
  • Author(s): Iwamoto, H.
  • Journal Title: Journal of Molecular Biology 369 Pages: 249-264
  • Peer Reviewed
• [Journal Article] The architecture of outer dynein arms in situ. (2007)
  • Author(s): Ishikawa, T.
  • Journal Title: Journal of Molecular Biology 368 Pages: 1249-1258
  • Peer Reviewed
• [Journal Article] Molecular and nanometer-scale self-organized system generated by protein motor functions. (2007)
  • Author(s): Oiwa, K.
  • Journal Title: Material Science Forum 539-543 Pages: 3290-3296
  • Peer Reviewed
• [Journal Article] Molecular and nanometer-scale self-organized system generated by protein motor functions. (2007)
  • Author(s): Oiwa, K.
  • Journal Title: Materials Science Forum 539-543 Pages: 3290-3296
• [Journal Article] 電子顕微鏡観察が明らかにするダイニン腕の構造と運動機構. (2007)
  • Author(s): 大岩和弘
  • Journal Title: 蛋白質・核酸・酵素 52・1 Pages: 37-43
  • NAID: 40015220361
• [Journal Article] Striated muscle twitchin of bivalves has the 'catchability', the ability to bind thick filaments tightly to thin filaments, representing the catch state. (2006)
  • Author(s): Tsutsui, Y.
  • Journal Title: Journal of Molecular Biology 365 Pages: 325-332
• [Journal Article] Swing or embrace? New aspects of motility inspired by dynein structure in situ. (2006)
  • Author(s): Toba, S.
  • Journal Title: BIOforum Europe 10 Pages: 14-16
• [Journal Article] Plant villin, lily P-135-ABP, possesses G-actin binding activity and accelerates the polymerization and depolymerization of actin in a Ca^<2+>-sensitive manner. (2005)
  • Author(s): Yokota, E
  • Journal Title: Plant and Cell Physiology 46 Pages: 1690-1703
• [Journal Article] Recent progress in dynein structure and mechanism (2005)
  • Author(s): K.Oiwa
  • Journal Title: Current Opinion in Cell Biology 17 Pages: 98-103
• [Journal Article] Recent progress in dynein structure and mechanism. (2004)
  • Author(s): Oiwa, K., and Sakakibara, H.
  • Journal Title: Curr. Opin. Cell Biol. 17 Pages: 95-103
  • Peer Reviewed
• [Journal Article] The structure of dynein-c by negative stain electron microscopy (2004)
  • Author(s): Burgess, S. A., Sakakibara, H., Oiwa K., Kingh, P. J.
  • Journal Title: J. Struct. Biol 146 Pages: 205-215
  • Peer Reviewed
• [Journal Article] Chemomechanical coupling in F1-ATPase revealed by simulataneous observation of nucleotide kinetics and rotation (2004)
  • Author(s): Nishizaka, T., Oiwa, K., Noji, H., Kimura, S., Muneyuki, E., Yoshida, M., and Kinosita, K. Jr.
  • Journal Title: Nat. Struct. Mol. Biol. 11 Pages: 142-148
  • Peer Reviewed
• [Journal Article] Controlled Nanoscale Motion, Lecture Notes 41-63
  • Author(s): Oiwa, K.
  • Journal Title: Controlled Nanoscale Motion, Lecture Notes in Physics, Springer(Berlin,Heidelberg) 007
• [Presentation] 活性の異なる2つのモーター部を持つ軸糸ダイニンfの運動特性 (2009)
  • Author(s): 鳥羽 栞
  • Organizer: 2009年 生体運動研究合同班会議
  • Place of Presentation: 東京大学・東京
  • Year and Date: 2009-01-09
• [Presentation] Distinct roles of 1α and 1β heavy chains of the I1 inner arm dynein of Chlamydomonas flagella (2008)
  • Author(s): 鳥羽 栞
  • Organizer: 48th The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-16
• [Presentation] Helical rearrangement of the axonemal components in Chlamydomonas flagella coupled with Ca^<2+> concentrations revealed by X-ray fiber diffractions (2008)
  • Author(s): Hitoshi Sakakibara
  • Organizer: The 48th American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-14
• [Presentation] クロストークネットワーク実現のための二成分情報伝達系の再構築(Reassembly of parallel two-component systems for interspecies communication networks) (2008)
  • Author(s): 平林 美樹
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸、兵庫
  • Year and Date: 2008-12-12
• [Presentation] Laser trap nanometry with non-spherical shaped probe (2008)
  • Author(s): 小嶋 寛明
  • Organizer: 日本生物物理学会
  • Place of Presentation: 神戸、兵庫
  • Year and Date: 2008-12-05
• [Presentation] Distinct roles of 1α and 1β heavy chains of the inner arm dynein I1 of Chlamydomonas flagella (2008)
  • Author(s): 鳥羽 栞
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2008-12-05
• [Presentation] Variety in structures of inner arm dyneins in Chlamydomonas axonemes (2008)
  • Author(s): 大岩 和弘
  • Organizer: ERATO Workshop : Actin filaments : From structure to mechanisms
  • Place of Presentation: 名古屋大学、愛知
  • Year and Date: 2008-11-23
• [Presentation] Mechanical and enzymatic properties of the heterodimeric axonemal dynein-f (2008)
  • Author(s): S. Toba
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京
  • Year and Date: 2008-11-01
• [Presentation] Mechanical and enzymatic properties of the heterodimeric axonemal dynein-f (2008)
  • Author(s): 鳥羽 栞
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2008-11-01
• [Presentation] 生物に学ぶナノメートルスケールの情報処理・伝達システム-分子通信技術の研究開発の現状- (2008)
  • Author(s): 大岩 和弘
  • Organizer: 電子情報通信学会 2008ソサイエティ大会チュートリアル
  • Place of Presentation: 明治大学生田キャンパス、神奈川
  • Year and Date: 2008-09-17
• [Presentation] ナマコのキャッチ結合組織に働く新規硬化因子 (2008)
  • Author(s): 池谷 知昭
  • Organizer: 日本動物学会 第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス、福岡
  • Year and Date: 2008-09-05
• [Presentation] A dynamical network model inspired by molecular motors (2008)
  • Author(s): Jian-Qin Liu
  • Organizer: The 9th International Conference on Systems Biology
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2008-08-22
• [Presentation] Conformational changes of dynein arms in situ studied using X-ray diffractions of Chlamydomonas flagellar axonemes (2008)
  • Author(s): K. Oiwa
  • Organizer: Gordon Research Conference Muscle & Molecular Motors
  • Place of Presentation: Boston
  • Year and Date: 2008-06-29
• [Presentation] Conformational changes of dynein arms in situ studied using X-ray diffractions of Chlamydomonas flagellar axonemes (2008)
  • Author(s): 大岩 和弘
  • Organizer: Gordon Research Conference MUSCLE & MOLECULAR MOTORS
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-06-29
• [Presentation] ヘテロ2量体である鞭毛内腕ダイニン亜種fの運動特性 (2008)
  • Author(s): 鳥羽 栞
  • Organizer: 特定領域「生体ナノシステム」下田ダイニン分子研究会
  • Place of Presentation: 筑波大学下田臨海実験センター、静岡
  • Year and Date: 2008-06-11
• [Presentation] Fluorescence Based Single Molecule Imaging and Nanometry on Axonemal Dyneins (2008)
  • Author(s): K. Oiwa
  • Organizer: 7th International Weber Symposium 2008
  • Place of Presentation: Hawai
  • Year and Date: 2008-06-10
• [Presentation] Fluorescence-based single molecule imaging and nanometry on axonemal dyneins (2008)
  • Author(s): 大岩 和弘
  • Organizer: 7th International Weber Symposium 2008
  • Place of Presentation: Kauai, Hawaii
  • Year and Date: 2008-06-08
• [Presentation] Development of bio-nano-devices based on protein motors' function (2008)
  • Author(s): 大岩 和弘
  • Organizer: The 5th International Symposium on Organic Molecular Electronics (ISOME 2008)
  • Place of Presentation: 兵庫県立大学、兵庫
  • Year and Date: 2008-05-23
• [Presentation] X線繊維回折により捉えた鞭毛軸糸の構造変化 (2007)
  • Author(s): 鳥羽 栞
  • Organizer: 第45回目本生物物理学会年会
  • Place of Presentation: 横浜、神奈川
  • Year and Date: 2007-12-23
• [Presentation] Force-generating mechanism of axonemal dyneins inspired by cryoelectron tomography and x-ray fiber diffraction analysis (2007)
  • Author(s): K. Oiwa
  • Organizer: The Abstbury Series, University of Leeds
  • Place of Presentation: Leeds
  • Year and Date: 2007-11-28
• [Presentation] The heterodimeric axonemal dynein-f Its mechanical and enzymatic properties and possible roles in beating of flagellar axonemes (2007)
  • Author(s): Kotani, N.
  • Organizer: Internationa lConference on Molecular Mechanism of Intracellular Transport
  • Place of Presentation: 苫小牧、北海道
  • Year and Date: 2007-08-24
• [Presentation] Force generating mechanism of axonemal dyneins inspired by cryoelectron tomography and X-ray fiber diffraction analysis (2007)
  • Author(s): Oiwa, K.
  • Organizer: International Conference on Molecular Mechanism of Intracellular Transport
  • Place of Presentation: 苫小牧、北海道
  • Year and Date: 2007-08-24
• [Presentation] ヘテロ二量体である鞭毛内腕ダイニン亜種の運動特性 (2007)
  • Author(s): 鳥羽 栞
  • Organizer: 2008年生体運動研究合同班会議
  • Place of Presentation: 苫小牧、北海道
  • Year and Date: 2007-08-24
• [Presentation] Conformational changes of dynein-arms in situ studied using X-rayd diffraction of flagellar axonemes (2007)
  • Author(s): Oiwa, K.
  • Organizer: 6th EBSA European Biophysics Congress
  • Place of Presentation: London UK
  • Year and Date: 2007-07-17
• [Presentation] Forcegenerating mechanism of axonemal dyneins studied by cryoelectron tomography and X-ray fiber diffraction analysis (2007)
  • Author(s): Oiwa, K.
  • Organizer: Japan Partnership Awards Dynein Workshop
  • Place of Presentation: Bristol,UK
  • Year and Date: 2007-07-10
• [Presentation] 軸糸ダイニンの構造と運動機能:トモグラムとin vitro assaysからの知見 (2007)
  • Author(s): Oiwa, K.
  • Organizer: 特定領域研究 ダイニン分子研究会
  • Place of Presentation: 東京大学 東京
  • Year and Date: 2007-06-07
• [Presentation] The structure and mechanism of protein motors and their potential applications in the development of bio-nano-devices (2007)
  • Author(s): K. Oiwa
  • Organizer: D. B. Robinson Distinguished Speaker Lecture Series, University of Alberta
  • Place of Presentation: Edmonton, Canada
  • Year and Date: 2007-03-01
• [Presentation] Force generation mechanism of axonemal dynein inspired by the structure of isolated and in situ dyneins of Chlamydomonas axonemes (2006)
  • Author(s): K. Oiwa
  • Organizer: 2006 Biophysical Society Discussions, Molecular Motors : Point Counterpoint
  • Place of Presentation: Asilomar
  • Year and Date: 2006-10-20
• [Presentation] Force-generation mechanism of dynein of inspired by structures of isolated and in situ dyneins of Chlamydomonas (2006)
  • Author(s): K. Oiwa
  • Organizer: International Symposium on Bio-nanosystems
  • Place of Presentation: 仙台
  • Year and Date: 2006-09-03
• [Presentation] Structural studies on axonemal dynein-arms in chlamydomonas flagella by electron tomography (2005)
  • Author(s): T. Ishikawa
  • Organizer: International Workshop Dynein 2005, Molecular Mechanisms of Axonemal and Cytoplasmic Dyneins
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2005-10-31
• [Presentation] Raveling axonemal dyneins with single molecule and x-ray diffraction techniques (2005)
  • Author(s): K. Oiwa
  • Organizer: Motors & Cytoskeleton Conference
  • Place of Presentation: Hamburug-Blankenese
  • Year and Date: 2005-09-25
• [Presentation] Force generation mechanism of axonemal dyneins (2005)
  • Author(s): K. Oiwa
  • Organizer: Joint 61st Harden Conference/EMBO Workshop-Molecularmotors : structure and function
  • Place of Presentation: Cambridge
  • Year and Date: 2005-09-18
• [Presentation] Molecular mechanisms of AAA-proteins, FtsH and dynein studied (TIRF) microscopy (2005)
  • Author(s): K. Oiwa
  • Organizer: 6th International Weber Smposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine
  • Place of Presentation: Hawai
  • Year and Date: 2005-07-23
• [Book] Force-generating mechanisms of dynein revealed through single molecule studies. In single Molecule Biology (2009)
  • Author(s): Oiwa K. & Kojima, H.
  • Publisher: Elsevier Academic Press, London.
• [Book] How linear motor proteins work, In Lecture Notes in Physics, Controlled Nanoscale Motion. H. Linke and A. Mansson, editors. (2007)
  • Author(s): Oiwa, K. & Manstein, D. J.
  • Publisher: Springer/Berlin, Heidelberg, New York
• [Book] How linear motor proteins work. In Lecture Notes in Physics, Controlled Nanoscale Motion. (H. Linke and A. Mansson, editors) (2007)
  • Author(s): Oiwa, K.
  • Publisher: Springer/Berlin, Heidelberg, New York.
• [Remarks] ホームページ等
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• [Patent(Industrial Property Rights)] 繊維状タンパク質の誘導素子及びその製造方法 (2006)
  • Inventor(s): 大岩和弘, 鈴木仁, 米谷玲皇, 松井真二
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Filing Date: 2006-06-29
• [Patent(Industrial Property Rights)] タンパク質モータの機能に基づく信号変換素子 (2006)
  • Inventor(s): 大岩和弘
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Acquisition Date: 2006-11-17
• [Patent(Industrial Property Rights)] タンパク質モータ用基板及びその製造方法とタンパク質モータ構造体 (2005)
  • Inventor(s): 大岩 和弘他3名
  • Industrial Property Rights Holder: 情報通信研究機構
  • Industrial Property Number: 2005-303390
  • Filing Date: 2005-10-18
• [Patent(Industrial Property Rights)] 生理活性を持つ環状ヌクレオチド誘導体及びその製造方法 (2004)
  • Inventor(s): 大岩和弘
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Acquisition Date: 2004-09-24