Nuclear dynamics

• Project/Area Number: 16084101
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: YONEDA Yoshihiro Osaka University, 大学院・生命機能研究科, 教授 (80191667)
• Co-Investigator(Kenkyū-buntansha): HIRAOKA Yasushi 大阪大学, 大学院・生命機能研究科, 教授 (10359078) ; HARAGUCHI Tokuko 独立行政法人情報通信研究機構, 未来ICT研究センター, 主任研究員 (20359079) ; TAKEYASU Kunio 京都大学, 大学院・生命科学研究科, 教授 (40135695) ; KIMURA Hiroshi 大阪大学, 大学院・生命機能研究科, 准教授 (30241392)
• Co-Investigator(Renkei-kenkyūsha): HIRAOKA Yasushi 大阪大学, 大学院・生命機能研究科, 教授 (10359078) ; HARAGUCHI Tokuko 独立行政法人情報通信研究機構, 未来ICT研究センター, 主任研究員 (20359079) ; TAKEYASU Kunio 京都大学, 大学院・生命科学研究科, 教授 (40135695) ; KIMURA Hiroshi 大阪大学, 大学院・生命機能研究科, 准教授 (30241392)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,900,000 (Direct Cost: ¥18,900,000); Fiscal Year 2008: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2007: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2006: ¥6,300,000 (Direct Cost: ¥6,300,000); Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2004: ¥4,500,000 (Direct Cost: ¥4,500,000)
• Keywords: 細胞核 / 分子イメージング / ゲノム / 細胞核内構造体 / 可視化技術 / 核-細胞質間輸送 / 染色体 / クロマチン / 細胞核内高次構造 / 核構造 / 光学顕微鏡技術 / 核膜病 / クロマチン高次構造 / 核膜孔複合体 / 核内ドメイン

Research Abstract

本研究領域は、真核細胞の遺伝情報を保持し、発現させる基本構造体である細胞核を研究対象とし、その機能を構造構築のダイナミクスに立脚して統合的に理解することを目的としている。このため、本総括班では、領域内での研究の有機的な融合のため、定期的に開催する班会議を最も重要視し、班会議での発表、討論に基づいて、内部評価を厳密に行なうとともに、活発な意見交換、情報交換を促すことにより、各研究者が相互に影響を及ぼし合い、学際的な相互交流が進展することを目指している。この目的に沿って、領域班会議を5回にわたって開催し、計画班・公募班を問わず、班員すべてが研究成果や計画を発表し、他の班員、総括班評価担当委員からの評価を受けた。また、総括班会議を8回にわたって開催し、主として本研究領域の活動をどのように総括し、次のステップにつなげていくかについて話し合ってきた。一方、本特定領域のキーワードの1つである「可視化」技術は、日進月歩で開発が進んでおり、それらの技術を用いたデータが蓄積してきているため、本研究領域独自のデータベース作成を行った。また、ホームページ、ニュースレターを通して情報発信に努めた。また、国際シンポジウムを3回、公開シンポジウムを2回主催し、国内外への情報発信と情報交換を行った。これら以外にも、技術講習会支援、ワークショップ等の後援など、積極的に本研究分野全体の発展に努めた。

Research Products

• [Journal Article] A high-resolution structure of the pre-microRNA nuclear export machinery. (2009)
  • Author(s): Okada, C., Yamashita, E., *Lee, S.J., Shibata, S., Katahira, J., Nakagawa, A., Yoneda, Y., Tsukihara, T.
  • Journal Title: Science 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase. (2009)
  • Author(s): Hayashi-Takanaka, Y., Yamagata, K., Nozaki, N.,, Kimura, H.
  • Journal Title: J. Cell Biol. 187 Pages: 781-790
  • Peer Reviewed
• [Journal Article] Nup98 nucleoporins bearing different types of repeat sequence characterize nucleus-specific nuclear pore complexes in the ciliate Tetrahymena. (2009)
  • Author(s): Iwamoto, M., Mori, C., Kojidani, T., Bunai, F., Hori, T., Fukagawa, T., Hiraoka, Y., Haraguchi, T.
  • Journal Title: Current Biol. 19 Pages: 843-847
  • Peer Reviewed
• [Journal Article] Infrared laser-mediated gene_inductlon in targeted single cells in vivo (2009)
  • Author(s): Kamei, Y., et.al.
  • Journal Title: Nature Methods 6 Pages: 79-81
  • Peer Reviewed
• [Journal Article] mDia2 shuttles between the nucleus and the cytoplasm through the Importin-α/β- and CRMl-mediated nuclear transport, mechanism (2009)
  • Author(s): Miki, T., et.al.
  • Journal Title: J. Biol. Chem. 284 Pages: 99-104
  • Peer Reviewed
• [Journal Article] Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA (2009)
  • Author(s): Katahira, I., et.al.
  • Journal Title: EMBO J (in press)
  • Peer Reviewed
• [Journal Article] Selective localization of PCBP2 to cytoplasmic processing bodies (2009)
  • Author(s): Fujimura, K., et.al.
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research (in press)
  • Peer Reviewed
• [Journal Article] Synergistic nuclear import of NeuroD1 and its partner transcription factor E47 via heterodimerization (2009)
  • Author(s): Mehmood, R., et.al
  • Journal Title: Exp. Cell Res. (in press)
  • Peer Reviewed
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA (2009)
  • Author(s): Katahira, J. and Yoneda, Y.
  • Journal Title: RNA Biol. (in press)
  • Peer Reviewed
• [Journal Article] The role of nuclear transport system in cell differentiation (2009)
  • Author(s): Yasuhara, N., et.al.
  • Journal Title: Seminars in Cell and Developmental Biology (in press)
  • Peer Reviewed
• [Journal Article] Individual binding pockets of importinβfor FG-nucleoporins are differently regulated by RanGTP. (2008)
  • Author(s): Otsuka, S., Iwasaka, S., Yoneda, Y., Takeyasu, K., Yoshimura, S.H.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 16101-16106
  • Peer Reviewed
• [Journal Article] Live cell imaging and electron microscopy revealed dynamic processes of BAF-directing nuclear envelope assembly. (2008)
  • Author(s): Haraguchi, T., Kojidani, T., Koujin, T., Shimi, T., Osakada, H., Mori, C., Yamamoto, A., Hiraoka, Y.
  • Journal Title: J. Cell Sci. 121 Pages: 2540-2554
  • Peer Reviewed
• [Journal Article] Generation and characterization of a monoclonal antibody against NPI-1, subfamily importin α (2008)
  • Author(s): Tachibana, T., et.al.
  • Journal Title: Hybridoma 27 Pages: 285-289
  • Peer Reviewed
• [Journal Article] Localized regulation of aminal RanGTPase controls retrograde injury signaling in, peripheral nerve (2008)
  • Author(s): Yudin, D., et.al.
  • Journal Title: Neuron 59 Pages: 241-252
  • Peer Reviewed
• [Journal Article] Individual binding pockets of importin β for FG-nucleoporins are differently regulated_by RanGTP (2008)
  • Author(s): Otsuka, S., et.al.
  • Journal Title: Proc. Natl. Acad. Sel. USA 105 Pages: 16101-16106
  • Peer Reviewed
• [Journal Article] Characterization of human herpesvirus 6 U69 gene product and identification of its nuclear localization signal. (2008)
  • Author(s): Isegawa, Y., et. al.
  • Journal Title: J. Virol. 82 Pages: 710-718
  • Peer Reviewed
• [Journal Article] Nuclear RNA export factor 7 is localized in processing bodies and neuronal mRNA granules through interactions with shuttling hnRNPs. (2008)
  • Author(s): Katahira, J., et. al.
  • Journal Title: Nucl. Acids Res. 36 Pages: 616-628
  • Peer Reviewed
• [Journal Article] Triggering neural differentiation of ES cells by subtype switching of importin-α. (2007)
  • Author(s): Yasuhara, N., Shibazaki, N., Tanaka, S., Nagai, M., Kamikawa, Y., Oe, S., Kamachi, Y., Kondoh, H., Yoneda, Y.
  • Journal Title: Nature Cell Biol. 9 Pages: 72-79
  • Peer Reviewed
• [Journal Article] Nuclear localization of barrier-to-autointegration factor is correlated with progression of S-phase in human cells. (2007)
  • Author(s): Haraguchi, T., Koujin, T., Osakada, H., Kojidani, T., Mori, C., Masuda, H., Hiraoka, Y.
  • Journal Title: J. Cell Sci. 120 Pages: 1967-1977
  • Peer Reviewed
• [Journal Article] NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins. (2007)
  • Author(s): Takano, K., et. al.
  • Journal Title: Nucl. Acids Res. 35 Pages: 2513-2521
  • Peer Reviewed
• [Journal Article] A novel nuclear import of Vpr promoted by importin A ia crucial for HIV-1 replication in macrophages. (2007)
  • Author(s): Nitahara-Kasahara, Y., et. al.
  • Journal Title: J. Virol. 81 Pages: 5284-5293
  • Peer Reviewed
• [Journal Article] Arx1 functions as an unorthodox nuclear export receptor for the 60Spre-ribosomal subunit. (2007)
  • Author(s): Bradatsch, B., et. al.
  • Journal Title: Mol. Cell 27 Pages: 767-779
  • Peer Reviewed
• [Journal Article] Normal brain development in importin-A5 deficient-mice. (2007)
  • Author(s): Yasuhara, N., et. al.
  • Journal Title: Nature Cell Biol. 9 Pages: 1339-1339
  • Peer Reviewed
• [Journal Article] 細胞分化を制御する核輸送因子importin α (2007)
  • Author(s): 安原徳子、米田悦啓
  • Journal Title: 蛋白質核酸酵素 52 Pages: 427-433
  • NAID: 40015447931
  • Peer Reviewed
• [Journal Article] A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A-H2B. (2006)
  • Author(s): Kimura, H., Takizawa, N., Allem,, N., Hori, T., Iborra, F.J., Nozaki, N., Muraki, M., Hagiwara, M., Krainer, A.R., Fukagawa, T., Okawa, K.
  • Journal Title: J. Cell Biol. 175 Pages: 389-400
  • Peer Reviewed
• [Journal Article] Meiotic proteins Bqt1 and Bqt2 tether telomeres to form the bouquet arrangement of chromosomes. (2006)
  • Author(s): Chikashige, Y., Tsutsumi, C., Yamane, M., Okamasa, K., Haraguchi, T., Hiraoka, Y.
  • Journal Title: Cell 125 Pages: 59-69
  • Peer Reviewed
• [Journal Article] Single-molecule reaction analysis reveals the ATP-ADP-dependent conformational changes of chaperonin GroEL. (2006)
  • Author(s): M. Yokokawa, C. Wada, T., o, N. Sakai, A. Yagi, Takeyasu, K.
  • Journal Title: EMBO J. 25 Pages: 4567-4576
  • Peer Reviewed
• [Journal Article] Importinαtransports Ca2+/calmodulin-dependent protein kinase type IV (CaMKIV) to the nucleus without utilizing importinβ. (2005)
  • Author(s): Kotera, I., Sekimoto, T., Miyamoto, Y., Saiwaki, T., Nagoshi, E., Sakagami, H., Kondo, H., Yoneda, Y.
  • Journal Title: EMBO J. 24 Pages: 942-951
  • Peer Reviewed
• [Journal Article] Linker histone H1 per se can induce three-dimensional folding of chromatin fiber. (2005)
  • Author(s): Hizume, K., Yoshimura, S.H., Takeyasu, K.
  • Journal Title: Biochemistry 44 Pages: 12978-12989
  • Peer Reviewed
• [Journal Article] The 70 kDa heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors. (2005)
  • Author(s): Kose, S., Furuta, M., Koike, M., Yoneda, Y., Imamoto, N.
  • Journal Title: J. Cell Biol. 171 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Cellular stresses induce the nuclear accumulation of importin-alpha and cause a conventional nuclear import block. (2004)
  • Author(s): Miyamoto, Y., Saiwaki, T., Yamashita, J., Yasuda, Y., Kotera, I., Shibata, S., Shigeta, M., Hiraoka, Y., Haraguchi, T., Yoneda, Y.
  • Journal Title: J. Cell Biol. 165 Pages: 617-623
  • Peer Reviewed
• [Presentation] Nuclear protein transport and cell function. (Plenary talk) 7th Annual Scientific Meeting of ARC Centre of Excellence in Biotechnology and Development (2009)
  • Author(s): Yoneda, Y.
  • Organizer: Kalorama, Victoria, Australia
• [Presentation] Nano-biology of the nucleus. (Keynote Speech) The 10th AEARU Workshop on Molecular Biology and Biotechnology (2009)
  • Author(s): Takeyasu, K.
  • Organizer: National Taiwan Univ.
  • Place of Presentation: Taiwan
• [Presentation] C2C12細胞の筋分化における核膜孔複合体リモデリング (2009)
  • Author(s): Asally, M, Yasuda, Y, Oka, M, Otsuka, S, Yoshimura, SH, YoiLeda, Y
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
• [Presentation] The modification and dynamics of histones in human cells as revealed by a panel of specific monoclonal antibodies. ICHC2008 (2008)
  • Author(s): Kimura, H.
  • Organizer: 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry, Symposium "Imaging of nuclear structure and dynamics"
  • Place of Presentation: Gdansk, Poland
• [Presentation] Nucleocytoplasmic transport of proteins and RNAs and its sig nificance.to cell function (2008)
  • Author(s): Yoneda, Y., Katahira, J., Sekimoto, T., Yasuhara, N., and Oka, M.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] Functional differences between two isoforms of Npap60(Nup50)in nuclear impiort (2008)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M., Asally, M. and Yoneda, Y.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] Nuclear accumulation of importin a suppresses DNA replication (2008)
  • Author(s): Mizuguchi, C., Yasuda, Y. and Yoneda, Y.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] Analysis of potential roles of Nup98 FG repeat region in oncogenesis (2008)
  • Author(s): Masahiro Oka, Munehiro Asally, Yoshinari Yasuda, Yoshihiro Yoneda
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] Iaitificdation of a cis-acting dendritic targeting element in the brain-derived neurotrophic factor mRNA (2008)
  • Author(s): Souichi Oe, Yoshihiro Yoneda
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] Nuclear protein import and its significance to cell function (2008)
  • Author(s): Yoneda, Y.
  • Organizer: 2nd. Intarnational_SOX Meeting
  • Place of Presentation: 兵庫
• [Presentation] Regulation of importin aLphal expression (2008)
  • Author(s): Yasunao Kamikawa, Noriko Yasehura, Yoshihiro Yoaneda
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
• [Presentation] A novel regulatory system to maintain ES cells through nuclear-cytoplasmic transport (2008)
  • Author(s): Yasuhara, N, Memood, R, Kamikawa, Y., Touma, K. and Yoneda, Y.
  • Organizer: 2nd Intetnational SOX Meeting
  • Place of Presentation: 兵庫
• [Presentation] Nuclear-cytoplasmic transport regulation of transcription factors (2008)
  • Author(s): Memood, R., Yoshikawa, K., Shibazaki, N., Kaneko, A., Yasuhara, N., Kondoh, H and Yoneda, Y.
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
• [Presentation] Nuclear import mechanism of Nanog (2008)
  • Author(s): Gibo, R., Okada, M., Yasuh ara. N. And Yoneda, Y.
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
• [Presentation] Selective localization of PCBP2 to P-bodies-An implication for functional diversity among P-bodies (2008)
  • Author(s): Ken Fujimura, Jun Katahira, Fumi Kano, Yoshihiro Yoneda, Masayuki, Marata
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
• [Presentation] Nuclear pore dynamics during skeletal myogenesis (2008)
  • Author(s): Asally, M., Yasuda,Y., Oka, M., Otsuka,S., Yoshimura, S. H., Yoneda,Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
• [Presentation] Nuclear import mechanism of neuroDl, E47 and their heterodimeric complex (2008)
  • Author(s): Mehmood, R., Yasuhara, N., Oe,S., Nagai,M., Yoneda, Y
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
• [Presentation] The role of Nup98 in a Crml-mediated nuclear export (2008)
  • Author(s): Oka,M., Asally, M., Yasuda, Y., Yoneda,Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
• [Presentation] Functional analysis of nuclear importin a accumulating in response to the stress (2008)
  • Author(s): Yasuda, Y., Miyamoto, Y., Yoneda, Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
• [Presentation] Nucleo-cytoplasmic transport and the assembly of stress granules (2008)
  • Author(s): Ken Fujimura, Jun Katahira, Fumi Kano, Yoshihiro Yoneda, Masayuki Murata
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
• [Presentation] 核蛋白質輸送と生命機能 (2008)
  • Author(s): 米田悦啓
  • Organizer: BMB2008第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 精子細胞形成過程におけるimportin alphaの核内機能 (2008)
  • Author(s): 宮本洋一、安田善也、山城朋子、永井理博、米田悦啓、David Jans、 Kate Loveland
  • Organizer: BMB2008第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Fission yeast genes involved in meiotic SPB-telomere cluster formation. (2007)
  • Author(s): Chikashige, Y., Tsutsumi, C., Yamane, M., Okamasa, K., Haraguchi, T., Hiraoka, Y.
  • Organizer: Fourth International Fission Yeast Meeting
  • Place of Presentation: Institute of Public Health, Copenhagen, Denmark
  • Year and Date: 2007-06-13
• [Presentation] 核-細胞質間分子流通機構と生命機能の調節 (2007)
  • Author(s): 米田悦啓
  • Organizer: 第71回日本生化学会中部支部会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-05-19
• [Presentation] Importin alpha has multiple functions (2007)
  • Author(s): Yoneda, Y.
  • Organizer: EMBO Workshop on Mechanisms of Nucleocytoplasmic Transport
  • Place of Presentation: Italy
• [Presentation] Nucleocytoplasmic transport and its significance to nuclear function. (2006)
  • Author(s): Yoneda, Y.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress:
  • Place of Presentation: Kyoto
• [Presentation] Chromatin structures and dynamics in fission yeast meiosis. (2005)
  • Author(s): Hiraoka, Y.
  • Organizer: Gordon Research Conference of Chromosome Dynamics
  • Place of Presentation: Colby-Sawyer College, USA
  • Year and Date: 2005-07-31
• [Book] Modern Atomic Force Microscopy and Its Application to the Study of Genome Architecture (2009)
  • Author(s): Takeyasu, K., Maruyama, H., Suzuki, Y., Hizume, K., Yoshimura, S.H.(ed. B. Bhushan.)
  • Publisher: Springer-Verlag
• [Book] 分子生物学イラストレイテット第3版 (2009)
  • Author(s): 浅利宗弘、米田悦啓
  • Total Pages: 348
  • Publisher: 羊土社
• [Book] 標準細胞生物学 (2008)
  • Author(s): 片平じゅん、米田悦啓
  • Total Pages: 358
  • Publisher: 医学書院
• [Book] Nuclear Dynamics (2007)
  • Author(s): Saiwaki, T. Yoneda, Y.
  • Total Pages: 279
  • Publisher: Springer-Verlag Inc.
• [Book] 細胞核の世界-ダイナミクスから病態まで (2007)
  • Author(s): 米田悦啓、他
  • Total Pages: 383
  • Publisher: 共立出版
• [Book] Structure and function of inner-nuclear architectures. Nuclear Dynamics (2006)
  • Author(s): Kimura, H., Cook, P.R.(ed. K. Takeyasu, K. Nagata)
  • Publisher: Springer-Verlag
• [Book] Nuclear Import and Export in Plants and Animals (2005)
  • Author(s): Sekimoto, T., Katahira, J.,, Yoneda, Y.(eds. Tzfira Tzvi, Vitaly Citovsky)
  • Publisher: Landes Bioscience
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/
• [Remarks]
  • URL: http://www.nuclear-dynamics.jp/symposium/index.html
• [Patent(Industrial Property Rights)] Method of Measuring Biological Sample with high Accuracy (2008)
  • Inventor(s): Takeshi Shimi, Tokuko Haraguchi
  • Industrial Property Rights Holder: National Institute of Information Communications Technology
  • Acquisition Date: 2008-02-26
• [Patent(Industrial Property Rights)] 超迅速にDNA塩基配列を決定する方法 (2006)
  • Inventor(s): 小寺一平、永井健治、谷知巳、米田悦啓
  • Industrial Property Rights Holder: 小寺一平、永井健治、谷知巳、米田悦啓
  • Industrial Property Number: 2006-034211
  • Filing Date: 2006-02-20
• [Patent(Industrial Property Rights)] 生物試料を高精度に測定する方法 (2006)
  • Inventor(s): 志見剛、原口徳子
  • Industrial Property Rights Holder: 独立行政法人情報通信研究機構
  • Acquisition Date: 2006-11-17