Molecular mechanisms of chromatin formation and dynamics

• Project/Area Number: 16084202
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University (2007-2008); Kyoto University (2004-2006)
• Principal Investigator: KIMURA Hiroshi Osaka University, 生命機能研究科, 准教授 (30241392)
• Co-Investigator(Kenkyū-buntansha): OHSUMI Keita 東京工業大学, 生命理工学研究科, 准教授 (20221822)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥130,000,000 (Direct Cost: ¥130,000,000); Fiscal Year 2008: ¥23,500,000 (Direct Cost: ¥23,500,000); Fiscal Year 2007: ¥24,300,000 (Direct Cost: ¥24,300,000); Fiscal Year 2006: ¥24,300,000 (Direct Cost: ¥24,300,000); Fiscal Year 2005: ¥24,300,000 (Direct Cost: ¥24,300,000); Fiscal Year 2004: ¥33,600,000 (Direct Cost: ¥33,600,000)
• Keywords: 染色体構築 / 機能 / 分配、核構造、細胞構造 / 機能、細胞周期、クロマチン / アフリカツメガエル / クロマチン / ヒストン / 細胞周期 / 膜透過化細胞 / 無細胞系 / 翻訳後修飾 / シャペロン / 細胞核 / 染色体 / 遺伝子発現 / ゲノム恒常性 / DNA複製 / ヌクレオソーム / アフリカヅメガエル / 細胞生物学 / 細胞 / 分子生物学

Research Abstract

本研究は、細胞核内のクロマチンダイナミクスの意義と制御機構を明らかにすることを目的として行われた。膜透過化細胞やアフリカツメガエル卵抽出液を用いた再構成系を用いて、ヒストンの交換やアセンブリに働く因子の同定と解析を行い、ゲノムの恒常性維持や複製における各ヒストンシャペロンの機能を明らかにした。また、生きた細胞におけるヒストン修飾のダイナミクスの解析法を確立した。

Research Products

• [Journal Article] G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. (2008)
  • Author(s): Tachibana, M., Matsumura, Y., Fukuda, M., Kimura, H. and Shinkai, Y.
  • Journal Title: EMBO J. 27 Pages: 2681-2690
  • Peer Reviewed
• [Journal Article] Alterations of lysine modifications on histone H3 N-tail under drought stress conditions in Arabidopsis thaliana. (2008)
  • Author(s): Kim, J.-M., Kim To, T., Ishida, J., Morosawa, T., Kawashima, M., Matsui, A., Toyoda, T., Kimura, H., Shinozaki, K. and Seki, M.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1580-1588
  • NAID: 10027334715
  • Peer Reviewed
• [Journal Article] Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro. (2008)
  • Author(s): Tachiwana, H., Osakabe, A., Kimura, H. and Kurumizaka, H.
  • Journal Title: Nucl. Acids Res. 36 Pages: 2208-2218
  • Peer Reviewed
• [Journal Article] Organization of histone H3 modifications revealed by a panel of specific monoclonal antibodies. (2008)
  • Author(s): Kimura, H., Hayashi-Takanaka, Y., Goto, Y., Takizawa, N. and Nozaki, N.
  • Journal Title: Cell Struct. Funct. 33 Pages: 61-73
  • NAID: 40016794072
  • Peer Reviewed
• [Journal Article] MKKS is a centrosome-shuttling protein degraded by disease-causing mutations via CHIP-mediated ubiquitination. (2008)
  • Author(s): Hirayama, S., Yamazaki, Y., Kitamura, A., Oda, Y., Morito, D., Okawa, K., Kimura, H., Cyr, D.M., Kubota, H. and Nagata, K.
  • Journal Title: Mol. Biol. Cell 19 Pages: 899-911
  • Peer Reviewed
• [Journal Article] Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A. (2008)
  • Author(s): Yomoda, J., Muraki, M., Kataoka, N., Hosoya, T., Suzuki, M., Hagiwara, M. and Kimura, H.
  • Journal Title: Genes Cells 13 Pages: 233-244
  • Peer Reviewed
• [Journal Article] ビフォー&アフター フォトブリーチ (2008)
  • Author(s): 木村 宏
  • Journal Title: 蛋白質核酸酵素 53 Pages: 1992-1999
• [Journal Article] Alterations of lysine modifications on histone H3 N-tail under drought stress conditions in Arabidopsis thaliana (2008)
  • Author(s): Kim, J. M
  • Journal Title: Plant Cell Physiology 49 Pages: 1580-1588
  • Peer Reviewed
• [Journal Article] G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription (2008)
  • Author(s): Tachibana, M
  • Journal Title: EMBO Journal 27 Pages: 2681-2690
  • Peer Reviewed
• [Journal Article] ビフォー & アフターフォトブリーチ (2008)
  • Author(s): 木村宏
  • Journal Title: 蛋白質核酸酵素 53 Pages: 1992-1999
  • Peer Reviewed
• [Journal Article] The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies (2008)
  • Author(s): Kimura, H.
  • Journal Title: Cell Structure and Function 33(In press)
  • NAID: 40016794072
  • Peer Reviewed
• [Journal Article] Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro (2008)
  • Author(s): Tachiwana, H.
  • Journal Title: Nucleic Acids Research (in press)
  • Peer Reviewed
• [Journal Article] MKKS is a centrosome-shutling protein degraded by disease-causing mutations via CHIP-mediated ubiqutination (2008)
  • Author(s): Hirayama, S.
  • Journal Title: Molecular Biology of the Cell 19 Pages: 899-911
  • Peer Reviewed
• [Journal Article] Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A (2008)
  • Author(s): Yomoda, J.i.
  • Journal Title: Genes to Cells 13 Pages: 233-244
  • Peer Reviewed
• [Journal Article] DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics. (2007)
  • Author(s): Ikura, T., Tashiro, S., Kakino, A., Shima, H., Jacob, N., Amunugama, R., Yoder, K., Izumi, S., Kuraoka, I., Tanaka, K., Kimura, H., Ikura, M., Nishikubo, S., Ito, T., Muto, A., Miyagawa, K., Takeda, S., Fishel, R., Igarashi, K. and Kamiya, K.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells. (2007)
  • Author(s): Murakawa, Y., Sonoda, E., Barber, L.J., Zeng, W., Yokomori, K., Kimura, H., Niimi, A., Lehmann, A., Zhao, G.U., Hochegger, H., Boulton, S.J. and Takeda, S.
  • Journal Title: Cancer Res. 67 Pages: 8536-8543
  • Peer Reviewed
• [Journal Article] Phosphorylation of Erp1 by p90rsk is required for cytostatic factor arrest in Xenopus laevis eggs. (2007)
  • Author(s): Nishiyama, T., Ohsumi, K., and Kishimoto, T.
  • Journal Title: Nature 446 Pages: 1096-1099
  • Peer Reviewed
• [Journal Article] Transient activation of calcineurin is essential to initiate embryonic development in Xenopus laevis. (2007)
  • Author(s): Nishiyama, T., Yoshizaki, N., Kishimoto, T. and Ohsumi, K.
  • Journal Title: Nature 449 Pages: 341-345
  • Peer Reviewed
• [Journal Article] 光退色と光刺激による細胞内分子動態の解析 (2007)
  • Author(s): 木村宏
  • Journal Title: 化学と生物 45 Pages: 798-804
  • NAID: 10019767889
• [Journal Article] DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics (2007)
  • Author(s): Ikuta, T.
  • Journal Title: Molecular and Cellular Biology 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] Inhibitors of the proteasome suppress homologous DNA recomvination in mammalian cells (2007)
  • Author(s): Murakawa, Y.
  • Journal Title: Cancer Research 67 Pages: 8536-8543
  • Peer Reviewed
• [Journal Article] Histone H1 variant, H1R is involved in DNA damage response (2007)
  • Author(s): Hashimoto, H.
  • Journal Title: DNA Repair 6 Pages: 1584-1595
  • Peer Reviewed
• [Journal Article] The interaction between Cap-binding complex and RNA export factor is required for intronless mRNA export (2007)
  • Author(s): Nojima, T.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 15645-15651
  • Peer Reviewed
• [Journal Article] A second proliferating cell nuclear antigen loader complex, Ctf-18-replication factor C, stimulates DNA polymerase eta activity (2007)
  • Author(s): Shiomi, Y.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 20906-20914
  • Peer Reviewed
• [Journal Article] Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts (2007)
  • Author(s): Miyamoto, K.
  • Journal Title: Molecular Reproduction and Development 74 Pages: 1268-1277
  • Peer Reviewed
• [Journal Article] Phosphorylation of Erp1 by P90rsk is required for cyctostatic factor arrest in Xenopus laevis eggs (2007)
  • Author(s): Nishiyama, T.
  • Journal Title: Nature 446 Pages: 1096-1099
  • Peer Reviewed
• [Journal Article] Transient activation of calcineurin is essential to initiate cmbryonic development in Xenopus laevis (2007)
  • Author(s): Nishiyama, T.
  • Journal Title: Nature 449 Pages: 341-345
  • Peer Reviewed
• [Journal Article] 光退色と光刺激による細胞内分子動態の解析 (2007)
  • Author(s): 木村 宏
  • Journal Title: 化学と生物 45 Pages: 798-804
  • NAID: 10019767889
• [Journal Article] The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export (2007)
  • Author(s): Nojima, T.et al.
  • Journal Title: Journal of Biological Chemistry (in press)
• [Journal Article] Phosphorylation of Erpl by p90rsk is required for cytostatic factor arrest in Xenopus laevis eggs (2007)
  • Author(s): Nishiyama, T.et al.
  • Journal Title: Nature (in press)
• [Journal Article] A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A-H2B. (2006)
  • Author(s): Kimura, H., Takizawa, N., Allemand, N., Hori, T., Iborra, F.J., Nozaki, N., Muraki, M., Hagiwara, M., Krainer, A.R., Fukagawa, T. and Okawa, K.
  • Journal Title: J. Cell Biol. 175 Pages: 389-400
  • Peer Reviewed
• [Journal Article] Cytosolic chaperonin prevents polyglutamine toxicity with altering aggregation state. (2006)
  • Author(s): Kitamura, A., Kubota, H., Pack, C.G., Matsumoto, G., Hirayama, S., Takahashi, Y., Kimura, H., Kinjo, M., Morimoto, R.I. and Nagata, K.
  • Journal Title: Nature Cell Biol. 8 Pages: 1163-1169
  • NAID: 120005549596
  • Peer Reviewed
• [Journal Article] Oocyte extracts for the study of meiotic M-M transition. (2006)
  • Author(s): Ohsumi, K., Yamamoto, T. M. and Iwabuchi M.
  • Journal Title: Methods Mol. Biol. 322 Pages: 445-458
• [Journal Article] 細胞周期と細胞分裂のダイナミクス : オーバービュー (2006)
  • Author(s): 木村宏
  • Journal Title: 蛋白質核酸酵素 51 Pages: 2115-2116
• [Journal Article] 細胞核の解析技術 : オーバービュー (2006)
  • Author(s): 木村宏
  • Journal Title: 蛋白質核酸酵素 51 Pages: 1970-1971
• [Journal Article] クロマチン機能の頑強性と可塑性を支える細胞核構造 (2006)
  • Author(s): 木村宏
  • Journal Title: 蛋白質核酸酵素 51 Pages: 293-300
• [Journal Article] A novel histone exchange factor, protein phosphatase 2Cg, mediatets the exchange and dephosphorylation of H2A-H2B (2006)
  • Author(s): Kimura, H.et al.
  • Journal Title: Journal of Cell Biology 175(3) Pages: 389-400
• [Journal Article] Cytosolic chaperonin prevents polyglutamine toxicity with altering the aggregation state (2006)
  • Author(s): Kitamura, A.et al.
  • Journal Title: Nature Cell Biology 8(10) Pages: 1163-1170
• [Journal Article] 細胞核の解析技術 : オーバービュー (2006)
  • Author(s): 木村 宏
  • Journal Title: 蛋白質核酸酵素 51(14) Pages: 1970-1971
• [Journal Article] 細胞周期と細胞分裂のダイナミクス:オーバービュー (2006)
  • Author(s): 木村 宏
  • Journal Title: 蛋白質核酸酵素 51(14) Pages: 2115-2116
• [Journal Article] Histone dynamics in living cells revealed by photobleaching. (2006)
  • Author(s): Kimura, H.
  • Journal Title: DNA Repair 4(8) Pages: 939-950
• [Journal Article] クロマチン機能の頑強性と可塑性を支える細胞核構造 (2006)
  • Author(s): 木村 宏
  • Journal Title: 蛋白質核酸酵素 51(4) Pages: 293-300
• [Journal Article] Oocyte extracts for the study of meiotic M-M transition. (2006)
  • Author(s): Ohsumi, K. et al.
  • Journal Title: Methods in Molecular Biology 332 Pages: 445-458
• [Journal Article] The constitutive centromere component CENP-50 is required for recovery from spindle damage. (2005)
  • Author(s): Minoshima, Y., Hori, T., Okada, M., Kimura, H., Haraguchi, T., Hiraoka, Y., Bao, Y.C., Kawashima, T., Kitamura, T. and Fukagawa, T.
  • Journal Title: Mol. Cell. Biol. 25 Pages: 10315-10328
  • Peer Reviewed
• [Journal Article] The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis. (2005)
  • Author(s): Mikami, Y., Hori, T., Kimura, H. and Fukagawa, T.
  • Journal Title: Mol. Cell. Biol. 25 Pages: 1958-1970
  • Peer Reviewed
• [Journal Article] Histone dynamics in living cells revealed by photobleaching. (2005)
  • Author(s): Kimura, H.
  • Journal Title: DNA Repair 4 Pages: 939-950
  • Peer Reviewed
• [Journal Article] Linker histone variants control chromatin dynamics during early embryogenesis. (2005)
  • Author(s): Saeki, H, Ohsumi, K., Aihara, H., Ito, T., Hirose, S., Ura, K. and Kaneda, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 5697-57
  • Peer Reviewed
• [Journal Article] Nucleosome assembly protein-1 is a linker histone chaperone in Xenopus eggs. Proc. (2005)
  • Author(s): Shintomi, K., Iwabuchi, M., Saeki, H., Ura, K., Kishimoto, T. and Ohsumi, K.
  • Journal Title: Natl. Acad. Sci. USA 102 Pages: 8210-8215
  • Peer Reviewed
• [Journal Article] The constitutive centromere component CENP-50 is required for recovery from spindle damage. (2005)
  • Author(s): Minoshima, Y. et al.
  • Journal Title: Molecular and Cellular Biology 25(23) Pages: 10315-10328
• [Journal Article] Linker histone variants control chromatin dynamics during early emryogenesis. (2005)
  • Author(s): Saeki, H. et al.
  • Journal Title: Proceedings of the National Academy of Science of the United States of America 120(6) Pages: 5697-5702
• [Journal Article] Nucleosome assembly protin-1 is a linker histone chaperone in Xenopus eggs. (2005)
  • Author(s): Shintomi, K. et al.
  • Journal Title: Proceedings of the National Academy of Science of the United States of America 102(23) Pages: 8210-8215
• [Journal Article] フォトブリーチング法による蛍光標識蛋白質の細胞内動態解析法(2) : 生細胞における測定と定量的解析のためのアプローチ (2004)
  • Author(s): 木村宏
  • Journal Title: 実験医学 13 Pages: 1851-1856
• [Journal Article] フォトブリーチング法による蛍光標識蛋白質の細胞内動態解析法(1) : 固定細胞を用いた条件設定 (2004)
  • Author(s): 木村宏
  • Journal Title: 実験医学 13 Pages: 1739-1745
• [Journal Article] 時間軸にわたる染色体機能の変化 (2004)
  • Author(s): 木村宏
  • Journal Title: 医学のあゆみ 208 Pages: 890-894
• [Journal Article] Manipulation of alternative splicing by a newly developed inhibitor of Clks. (2004)
  • Author(s): Muraki, M, Ohkawara, B., Hosoya, T., Onogi, H., Koizumi, J., Koizumi, T., Sumi, K., Yomoda, J., Murray, M.V., Kimura H., Furuichi, K., Shibuya, H., Krainer, A.R., Suzuki, M., and Hagiwara, M.
  • Journal Title: J. Biol. Chem. 279 Pages: 24246-24254
  • Peer Reviewed
• [Journal Article] The functional organization of mitochondrial genomes in human cells. (2004)
  • Author(s): Iborra, F.J., Kimura H. and Cook P.R.
  • Journal Title: BMC Biol. 2 Pages: 9-9
  • Peer Reviewed
• [Journal Article] TNFalpha-induced ATF3 expression is bidirectionally regulated by the JNK and ERK pathways in vascular endothelial cells. (2004)
  • Author(s): Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H. and Hagiwara, M.
  • Journal Title: Genes Cells 9 Pages: 59-70
  • Peer Reviewed
• [Journal Article] Measuring histone and polymerase dynamics in living cells. (2004)
  • Author(s): Kimura, H., Hieda, M. and Cook, P.R.
  • Journal Title: Methods Enzymol. 375 Pages: 381-93
• [Presentation] Live cell imaging of histone H3 phosphorylation in G2/M phase (2009)
  • Author(s): Hiroshi Kimura
  • Organizer: Mitosis and Cancer Symposium
  • Place of Presentation: アムステルダム(オランダ)
  • Year and Date: 2009-02-26
• [Presentation] Live-cell imaging of histone H3 phosphorylation in G2/M phase. (2009)
  • Author(s): Y. Hayashi-Takanaka, N. Nozaki, and H. Kimura
  • Organizer: Mitosis and Cancer Symposium
  • Place of Presentation: Amsterdam, The Netherlands
• [Presentation] Dynamics of histone modifications in living cells (2008)
  • Author(s): Hiroshi Kimura
  • Organizer: International Symposium Decoding Epigenetic Code
  • Place of Presentation: 東京
  • Year and Date: 2008-12-15
• [Presentation] ピストン修飾ダイナミクスの可視化 (2008)
  • Author(s): 木村宏
  • Organizer: クロマチン研究会-細胞核・染色体・クロマチンの機能構造構築と動態-
  • Place of Presentation: 三島
  • Year and Date: 2008-10-22
• [Presentation] The modification and dynamics of histones in human cells as revealed by a panel of specific antibodies (2008)
  • Author(s): Hiroshi Kimura
  • Organizer: ICHC2008(XIII International Congress of Histochemistry and Cytochemistry)
  • Place of Presentation: グダンスク(ポーランド)
  • Year and Date: 2008-08-26
• [Presentation] Histone modifications associated with transcription activation and silencing as revealed by a panel of specific monoclonal antibodies (2008)
  • Author(s): Hiroshi Kimura
  • Organizer: 第2回日本エピジェネティクス研究会
  • Place of Presentation: コペンハーゲン(デンマーク)
  • Year and Date: 2008-08-19
• [Presentation] Dynamics of histone modifications during the cell cycle and gene activation (2008)
  • Author(s): Hiroshi Kimura
  • Organizer: International Symposium on Chromosome Dynamics in Ise
  • Place of Presentation: 志摩
  • Year and Date: 2008-05-28
• [Presentation] 転写の活性化に伴うヒストン修飾のダイナミクス (2008)
  • Author(s): 木村宏
  • Organizer: 第2回日本エピジェネティクス研究会
  • Place of Presentation: 三島
  • Year and Date: 2008-05-10
• [Presentation] Histone dynamics and modification in human cells. (2008)
  • Author(s): H. Kimura
  • Organizer: Millipore Bio Forum 2008
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-03-07
• [Presentation] 修飾ヒストン特異的モノクローナル抗体を用いたエビゲノム解析 (2008)
  • Author(s): 木村 宏
  • Organizer: 文部科学省科学研究費補助金特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」発足記念キックオフシンボジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-03-05
• [Presentation] クロマチン制御とがん (2008)
  • Author(s): 木村 宏
  • Organizer: 第10回癌治療増感研究シンボジウム
  • Place of Presentation: 奈良
  • Year and Date: 2008-02-10
• [Presentation] 細胞核のマニピュレーション:セミインタクト細胞を用いたin vitro転写誘導系の開発 (2008)
  • Author(s): 木村 宏
  • Organizer: 文部科学省科学研究費補助金特定領域研究「遺伝情報DECODE」・「細胞核ダイナミセス」合同シンポジウム 「クロマチンシグナリングの分子機構」
  • Place of Presentation: 東京
  • Year and Date: 2008-01-08
• [Presentation] 細胞周期と転写の活性化に伴うヒストン修飾のダイナミクス (2008)
  • Author(s): 木村 宏、林(高中) 陽子、野崎直仁
  • Organizer: BMB2008
  • Place of Presentation: 神戸
• [Presentation] Histone modifications associated with transcription activation and silencing as revealed by a panel of specific monoclonal antibodies. (2008)
  • Author(s): H. Kimura, Y. Hayashi-Takanaka, Y. Goto and N. Nozaki
  • Organizer: Benzon Symposium No. 55. Transcription
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] The modification and dynamics of histones in human cells as revealed by a panel of specific monoclonal antibodies. ICHC2008 (2008)
  • Author(s): H. Kimura, Y. Hayashi-Takanaka, and N. Nozaki
  • Organizer: 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry
  • Place of Presentation: Gdansk, Poland
• [Presentation] Dynamics of histone modifications during the cell cycle and gene activation. (2008)
  • Author(s): Y. Hayashi-Takanaka, N. Nozaki, and H. Kimura
  • Organizer: International Symposium on Chromosome Dynamics in Ise '08
  • Place of Presentation: Shima, Japan
• [Presentation] Meiosis-II arrest and its cancellation in Xenopus eggs. (2008)
  • Author(s): K. Ohsumi
  • Organizer: 12th Internatianal Xenopus Conference
  • Place of Presentation: Leiwen, Germany
• [Presentation] ヒストン交換と修飾のダイナミクス (2007)
  • Author(s): 木村 宏
  • Organizer: BMB2008
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-15
• [Presentation] 不活性X染色体上において、不活性化回避遺伝子はどのようにして不活性化を免れるのか (2007)
  • Author(s): 後藤 友二
  • Organizer: 第7回核ダイナミクス研究会
  • Place of Presentation: 札幌
  • Year and Date: 2007-09-25
• [Presentation] ヒストン交換と修飾のダイナミクス (2007)
  • Author(s): 木村 宏、林(高中)陽子、立和名博昭、越坂部晃永、胡桃坂仁志、野崎直仁
  • Organizer: BMB2007
  • Place of Presentation: 横浜
• [Presentation] A novel link between histone deposition and dephosphorylation through a protein phosphatase. (2007)
  • Author(s): H. Kimura, N. Takizawa, E. Allemand, T. Hori, F.J. Iborra, N. Nozaki, M. Muraki, M. Hagiwara, A.R. Krainer, T. Fukagawa, and K. Okawa
  • Organizer: International Symposium on Functional Organization of the Nucleus
  • Place of Presentation: Awaji, Japan
• [Presentation] Transient activation of calcineurin is essential for MII exit in activated Xenopus eggs. (2007)
  • Author(s): T. Nishiyama, T. Kishimoto and K. Ohsumi
  • Organizer: EMBO World Workshop: 8th European Meiosis Meeting in Japan
  • Place of Presentation: Hayama, Japan
• [Presentation] Immobilization of importin alpha by annulate lamellae is required for survival of Xenopus laevis oocytes. (2007)
  • Author(s): K. Ohsumi
  • Organizer: International Symposium on Functional Organization of the Nucleus
  • Place of Presentation: Awaji, Japan
• [Presentation] Reconstitution of histone dynamics in vitro identifies a novel factor that mediates histone H2A/H2B exchange. (2005)
  • Author(s): H. Kimura, N. Takizawa, M. Muraki, and K. Okawa
  • Organizer: 第58回日本細胞生物学会大会
  • Place of Presentation: 大宮
• [Presentation] クロマチン構造変化 : 可視化から再構成へ (2004)
  • Author(s): 木村宏
  • Organizer: 放射線影響学会シンポジウム「放射線照射直後におけるクロマチン構造変化」
  • Place of Presentation: 長崎
• [Presentation] Reconstitution of histone dynamics in vitro. (2004)
  • Author(s): H. Kimura
  • Organizer: Cold Spring Harbor Laboratory meeting on Functional Organization of Nuclear Function.
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Histone dynamics and gene expression in living cells. (2004)
  • Author(s): H. Kimura
  • Organizer: 16th International Congress of International Federation of Associations of Anatomists
  • Place of Presentation: Kyoto, Japan
• [Presentation] Kinetics of histones in living human cells : Roles of H2A variants and factors that regulate histone exchange. (2004)
  • Author(s): H. Kimura
  • Organizer: The 1st Pacific-rim International Conference on Protein Science
  • Place of Presentation: Yokohama, Japan
• [Presentation] Cdc2 actvity is maintained at a constant level in CSF-arrested Xenopus eggs: Involovement of APC/C-Cdc20-mediated degradation of cyclin B but not Emi1. (2004)
  • Author(s): K. Ohsumi, T. Yamamoto and T. Kishimoto
  • Organizer: BSCB Autumn Meeting on Cell Cycle Regulation of Meiosis
  • Place of Presentation: Newcastle Upon Tyne, UK
• [Book] クロマチン免疫沈降(ChIP)法.「エピジェネティクス実験プロトコール(牛島俊和・眞貝洋一/編) (2008)
  • Author(s): 林(高中)陽子、後藤友二、木村宏
  • Publisher: 羊土社
• [Book] エピジェネティクス実験プロトコール(牛島俊和・眞貝洋一編) (2008)
  • Author(s): 林陽子
  • Total Pages: 267
  • Publisher: 羊土社
• [Book] 講義と実習 : 生細胞蛍光イメージング-阪大・北大顕微鏡コースブック- (2007)
  • Author(s): 原口徳子、木村宏、平岡泰
  • Publisher: 共立出版
• [Book] 講義と実習:生細胞蛍光イメージング 阪大・北大顕微鏡コースブック (2007)
  • Author(s): 原口徳子
  • Total Pages: 302
  • Publisher: 共立出版
• [Book] Nuclear Dynamics : Molecular Biology and Visualization of the Nucleus (Ed. K. Nagata and K. Takeyasu) (2006)
  • Author(s): Kimura, H., Cook, P.R.
  • Total Pages: 276
  • Publisher: Springer
• [Book] Nuclear Dynamics (Ed.K.Takeyasu and K.Nagata) (2006)
  • Author(s): Kimura, H. et al.
  • Publisher: Springer-Verlag Tokyo(印刷中)
• [Book] クロマチンタンパク質のFRAP解析 : 結合・解離速度の測定.「クロマチン・染色体実験プロトコール(押村光雄・平岡 泰/編) (2005)
  • Author(s): 木村宏
  • Publisher: 羊土社
• [Book] 転写の分子イメージング.「細胞核のダイナミクス(竹安邦夫・米田悦啓/編) (2004)
  • Author(s): 木村宏
  • Publisher: シュプリンガーフェアラーク東京
• [Book] Structure and function of inner-nuclear architectures. Nuclear Dynamics (ed. K. Takeyasu and K. Nagata)
  • Author(s): Kimura, H. and Cook, P.R.
  • Publisher: Springer-Verlag Tokyo, Tokyo.