Structural Dynamics of Nucleus: Analysis by Nano-scale Imaging
Project/Area Number |
16084203
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Kyoto University |
Principal Investigator |
TAKEYASU Kunio Kyoto University, 大学院・生命科学研究科, 教授 (40135695)
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Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Shigehiro 京都大学, 大学院・生命科学研究科, 准教授 (90346106)
HIZUME Kohji 京都大学, 大学院・生命科学研究科, 助教 (10378846)
HORIGOME Tsuneyoshi 新潟大学, 理学部, 教授 (60053352)
|
Project Period (FY) |
2004 – 2008
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Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥108,000,000 (Direct Cost: ¥108,000,000)
Fiscal Year 2008: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2007: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2006: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2005: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2004: ¥29,200,000 (Direct Cost: ¥29,200,000)
|
Keywords | 核内構造タンパク質 / 核膜孔 / 核膜再構成 / 核マトリックス / 核小体 / マトリックス / クロマチンファイバー / 染色体 / モノクローナル抗体 |
Research Abstract |
(1) 新しいナノスケール解析法の開発として、高速AFMの応用法の確立をした。また、効率的な「AFM-カンチレバーの修飾法」を確立し、細胞核動態の解析に応用した。 (2) 細胞核マトリックス画分はWD repeatsを持つタンパク質とdisordered領域を多く含むタンパク質(DO タンパク質)を高率で含むことを明らかにし、45種の新規タンパク質を同定した。また、新しい核マトリックス構造モデルとして"Dynamic scaffold-based model"を提案した。 (3) 各種核膜内膜タンパク質の分裂期におけるリン酸化とクロマチンからの解離の関係を明らかにした。
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Report
(6 results)
Research Products
(96 results)
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[Journal Article] Nuclear envelope precursor vesicle targeting to chromatin is stimulated by protein phosphatase 1 in Xenopus egg extracts.2007
Author(s)
Ito, H., Koyama, Y., Takano, M., Ishii, K., Maeno, M., Furukawa, K. and T. Horigome
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Journal Title
Exp. Cell Res. 313
Pages: 1897-1910
Related Report
Peer Reviewed
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[Journal Article] Anti-p97/VCP antibodies: an autoantibody marker for a subset of primary biliary cirrhosis patients with milder disease?2006
Author(s)
K. Miyachi, H. Hosaka, N. Nakamura, H. Miyakawa, T. Mimori, M. Shibata, S. Matsushima, H. Chinoh, T. Horigome, Hankins, W. R., Zhang, M., and Fritzler, J. M.
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Journal Title
Scand. J. Immun. 63
Pages: 376-382
Related Report
Peer Reviewed
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[Journal Article] Atomic force microscopy dissects the hierarchy of genome architectures in eukaryote, prokaryote and chloroplast.2006
Author(s)
R. L. Ohniwa, K. Morikawa, J. Kim, T. Kobori, K. Hizume, R. Matsumi, H. Atomi, T. Imanaka, T. Ohta, S. H. Yoshimura & K. Takeyasu
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Journal Title
Microscopy and Microanalysis 13
Pages: 3-12
Related Report
Peer Reviewed
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[Journal Article] Transcriptional co-activator PC4, a chromatin-associated protein, induces chromatin condensation.2006
Author(s)
C. Das, K. Hizume, K. Batta, B. R. P. Kumar, S. S.Gadad, S. Ganguly, S. Lorain, A. Verreault, P.. P. Sadhale, K. Takeyasu & T. K. Kundu
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Journal Title
Mol. Cell. Biol. 26
Pages: 8303-8315
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Peer Reviewed
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[Journal Article] Interaction of Skp1 with CENP-E at the midbody is essential for cytokinesis.2006
Author(s)
D. Liu, N. Zhang, J. Du, X. Cai, M. Zhu, C. Jin, Z. Dou, C. Feng, Y. Yang, L. Liu, K. Takeyasu, W. Xie & X. Yao
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Journal Title
Biochem. Biophys. Res. Commun. 345
Pages: 394-402
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Peer Reviewed
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[Journal Article] Transcriptional co-activator PC4, a chromatin-associated protein, induces chromatin condensation.2006
Author(s)
C.Das, K.Hizume, K.Batta, B.R.P.Kumar, S.S.Gadad, S.Ganguly, S.Lorain, A.Verreault, P.P.Sadhale, K.Takeyasu, T.K.Kundu
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Journal Title
Mol.Cell.Biol. 26
Pages: 1-13
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[Journal Article] Interaction of Skp1 with CENP-E at the midbody is essential for cytokinesis.2006
Author(s)
D.Liu, N.Zhang, J.Du, X.Cai, M.Zhu, C.Jin, Z.Dou, C.Feng, Y.Yang, L.Liu, K.Yakeyasu, W.Xie, X.Yao
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Journal Title
Biochem.Biophys.Res.Commun. 345
Pages: 394-402
Related Report
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[Journal Article] Atomic force microscopy dissects the hierarchy of genome architectures in eukaryote, prokaryote and chloroplast.2006
Author(s)
R.L.Ohniwa, K.Morikawa, J.Kim, T.Kobori, K.Hizume, R.Matsumi, H.Atomi, T.Imanaka, T.Ohta, S.H.Yoshimura, K.Takayasu
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Journal Title
Microscopy and Microanalysis. 13
Pages: 1-10
Related Report
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[Journal Article] Anti-p97/VCP antibodies : an autoantibody marker for a subset of primary biliary cirrhosis patients with milder disease?2006
Author(s)
Kiyomitsu Miyachi, Hiroo Hosaka, Noriko Nakamura, Hiroshi Miyakawa, Tsuneyo Mimori, Minoru Shibata, Shouzoh Matsushima, Hiroaki Chinoh, Tuneyoshi Horigome, Raleigh W.Hankins, Meifeng Zhang, Marvin J.Fritzler
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[Journal Article] Proteome analysis of rat liver, nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space2005
Author(s)
M. Segawa, K. Niino, R. Mineki, N. Kaga, K. Murayama, K. Sugimoto, Y. Watanabe, K. Furukawa,and T. Horigome
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Journal Title
FEBS J. 272
Pages: 4327-4338
Related Report
Peer Reviewed
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[Journal Article] Proteome analysis of rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space.2005
Author(s)
Segawa, M., Niino, K., Mineki, R., Kaga, N., Murayama, K., Sugimoto, K., Watanabe, Y., Furukawa, K., Horigome, T
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Journal Title
FEBS J 272
Pages: 4327-4338
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[Journal Article] Transcriptional Coactivator PC4, A Chromatin-associated Protein, Induces Chromatin Condensation.
Author(s)
Chandrima Das, Kiran Batta, Kohji Hizume, B.R.Prashanth Kumar, P.Deepa, Semanti Ganguly, Stephanie Lorain, Alain Verreault, Parag P.Sadhale Kunio Takeyasu, Tapas K.Kundu.
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