Regulation of nuclear domain localization and dynamics

• Project/Area Number: 16084205
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Hiroshima University
• Principal Investigator: TASHIRO Satoshi Hiroshima University, 原爆放射線医科学研究所, 教授 (20243610)
• Research Collaborator: CREMER Thomas ミュンヘン大学, 生物学部II, 教授
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥62,400,000 (Direct Cost: ¥62,400,000); Fiscal Year 2008: ¥11,700,000 (Direct Cost: ¥11,700,000); Fiscal Year 2007: ¥11,700,000 (Direct Cost: ¥11,700,000); Fiscal Year 2006: ¥11,700,000 (Direct Cost: ¥11,700,000); Fiscal Year 2005: ¥11,700,000 (Direct Cost: ¥11,700,000); Fiscal Year 2004: ¥15,600,000 (Direct Cost: ¥15,600,000)
• Keywords: 細胞核 / バイオイメージング / 核内ドメイン / イメージング

Research Abstract

DNA代謝制御に関わる核内ドメインの局在と動態の制御機構の解明に取り組んだ。その結果、転写因子Bach2が形成する核内ドメインの動態制御には蛋白質翻訳後修飾SUMO化修飾が重要であること、ヒストンH2AXのゲノム損傷依存的なアセチル化がゲノム修復関連核内ドメインRAD51フォーカスの形成制御に係わっていることが明らかになった。これらの研究から得られた知見から、核内ドメイン形成制御機構の一端が明らかになり、今後様々な疾患の新しい診断法、治療法の開発に結びつくことが期待される。

Research Products

• [Journal Article] The human actin-related protein hArp5: nucleo-cytoplasmic shuttling and involvement in DNA repair. (2009)
  • Author(s): Kitayama K, Kamo M, Oma Y, Matsuda R,Uchida T, Ikura T, Tashiro S, Ohyama T,Winsor B, Harata M.
  • Journal Title: Exp Cell Res 315(2) Pages: 206-217
  • Peer Reviewed
• [Journal Article] Histone H2AX participates the DNA damage-induced ATM activation through interaction with NBS1 (2009)
  • Author(s): Kobayashi J, Tauchi H, Chen B, Bruma S, Tashiro S, Matsuura S, Tanimoto K, Chen DJ, Komatsu K.
  • Journal Title: Biochem Biophys Res Commun 380 Pages: 752-757
  • Peer Reviewed
• [Journal Article] The human actin-related protein hArp5 : nucleo-cytoplasmic shuttling and involvement in DNA repair (2009)
  • Author(s): Kitayama K
  • Journal Title: Exp Cell Res 315 Pages: 206-217
  • Peer Reviewed
• [Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway (2008)
  • Author(s): Ishiai M, Kitao H, Smogorzewska A,Tomida J, Kinomura A, Uchida E, Saberi A,Kinoshita E, Kinoshita-Kikuta E, Koike T,Tashiro S, Elledge SJ, Takata M.
  • Journal Title: Nat Struct Mol Biol 15(11) Pages: 1138-1146
  • Peer Reviewed
• [Journal Article] Regulation of heme oxygenase-1 by transcription factor Bach1 in the mouse brain (2008)
  • Author(s): Sakoda E, Igarashi K, Sun J, Kurisu K and Tashiro S.
  • Journal Title: Neurosci letters 440 Pages: 160-165
  • Peer Reviewed
• [Journal Article] Morphologic effects of epithelial ion channels on the mouse uterus: differences between raloxifene analog (LY117018) and estradiol treatments (2008)
  • Author(s): Nobuzane T, Tashiro S, Kudo Y.
  • Journal Title: Am J Obstet Gynecol 194(4) Pages: 1-6
  • Peer Reviewed
• [Journal Article] The mobility of Bach2 nuclear foci is regulated by SUMO-1 modification (2008)
  • Author(s): Kono K, Harano Y, Hoshino H, Kobayashi M, Bazzet-Jones D, Muto A, Igarashi K, Tashiro S
  • Journal Title: Exp Cell Res 314 Pages: 903-913
  • Peer Reviewed
• [Journal Article] DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics (2008)
  • Author(s): Ikura T*, Tashiro S*, Kakino A, Shima H, Jacob N, Amunugama R, Yoder K, Izumi S, Kuraoka I, Tanaka K, Kimura H, Ikura M, Nishikubo S, Ito T, Muto A, Miyagawa K, Takeda S, Fishel R, Igarashi K, Kamiya K
  • Journal Title: Mol Cell Biol 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway (2008)
  • Author(s): Ishiai M
  • Journal Title: Nat Struct Mol Biol 15 Pages: 1138-1146
  • Peer Reviewed
• [Journal Article] Regulation of heme oxygenase-1 by transcription factor Bachl in the mouse brain (2008)
  • Author(s): Sakoda E
  • Journal Title: Neurosci Lett 440 Pages: 160-165
  • Peer Reviewed
• [Journal Article] Morphologic effects of epithelialion channels on the mouse uterus : differences between raloxifene analog (LY 117018) and estradiol treatments (2008)
  • Author(s): Nobuzane T
  • Journal Title: Am J Obstet Gynecol 199
  • Peer Reviewed
• [Journal Article] Corepressor SMRT and class II histone deacetylases promote Bach2 nuclear retention and formation of nuclear foci that are responsible for local transcriptional repression (2007)
  • Author(s): Hoshino H, Nishino TG, Tashiro S, Miyazaki M, Igarashi K, Horinouchi S and Yoshida M
  • Journal Title: J. Biochem 141 Pages: 719-727
  • NAID: 10020099472
  • Peer Reviewed
• [Journal Article] Nuclear positioning of the BACH2 gene in BCR-ABL positive leukemia cells (2007)
  • Author(s): Ono A, Kono K, Ikebe D, Muto A, Sun J, Kobayashi M, Ueda K, Melo JV, Igarashi K and Tashiro S
  • Journal Title: Genes, Chromosomes & Cancer 46 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Non-SCN5A related Brugada syndromes: verification of normal splicing and trafficking of SCN5A without exonic mutations (2007)
  • Author(s): Nakano Y, Tashiro S, Kinoshita E, Kinoshita-Kikuta E, Takenaka S, Miyoshi M, Ogi H, Sakoda E, Oda N, Suenari K, Tonouchi Y, Okimoto T, Hirai Y, Miura F, Yamaoka K, Koike T, Chayama K
  • Journal Title: Ann Hum Genet 71 Pages: 8-17
  • Peer Reviewed
• [Journal Article] The mobility of Bach2 nuclear foci is regulated by SUMO-1 modification (2007)
  • Author(s): Kono K
  • Journal Title: Exp CeH Res. 314 Pages: 903-913
  • Peer Reviewed
• [Journal Article] DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics (2007)
  • Author(s): Ikura T
  • Journal Title: Mol CeU Biol. 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] Corepressor SMRT and class II Mstone deacetylases promote Bach2nuclear retention and formation of nuclear foci that are responsible forlocal transcriptional repression. (2007)
  • Author(s): Hoshino H
  • Journal Title: J. Biochem. 141 Pages: 719-727
  • Peer Reviewed
• [Journal Article] Non-SCN5A related Brugada syndromes: verifcation of normal splicing and trafficking of SCN5A without exonic mutations (2007)
  • Author(s): Nakano Y
  • Journal Title: Ann Hum Genet. 71 Pages: 8-17
  • Peer Reviewed
• [Journal Article] Nuclear positioning of the BACH2 gene in BCR-ABL positive leukemic cells. (2007)
  • Author(s): Ono A et al.
  • Journal Title: Genes, Chromosomes Cancer 46 Pages: 67-74
• [Journal Article] Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells (2006)
  • Author(s): Ochiai K, Katoh Y, Ikura T, Hoshikawa Y, Noda T, Karasuyama H, Tashiro S, Muto A, Igarashi K
  • Journal Title: J. Biol. Chem 281 Pages: 38226-38234
  • Peer Reviewed
• [Journal Article] Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells. (2006)
  • Author(s): Ochiai K et al.
  • Journal Title: J Biol Chem 281 Pages: 38226-38234
• [Journal Article] Dynamic Cytoplasmic Anchoring of the Transcription Factor Bach1 by Intracellular Hyaluronic Acid Binding Protein IHABP (2005)
  • Author(s): Yamasaki C, Tashiro S, Nishito Y, Sueda T, Igarashi K
  • Journal Title: J Biochem (Tokyo) 137 Pages: 287-96
  • NAID: 10017343668
  • Peer Reviewed
• [Journal Article] Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1 (2005)
  • Author(s): Miyazaki M, Kawamoto H, Kato Y, Itoi M, Miyazaki K, Masuda K, Tashiro S, Ishihara H, Igarashi K, Amagai T, Kanno R, Kanno M
  • Journal Title: a target of the notch pathway.J Immunol. 174 174 Pages: 2507-2516
  • Peer Reviewed
• [Journal Article] Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the notch pathway. (2005)
  • Author(s): Miyazaki M et al.
  • Journal Title: J Immunol. 174 Pages: 2507-2516
• [Presentation] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot in therapy-related leukemia (2009)
  • Author(s): Satoshi Tashiro
  • Organizer: Keystone symposia on molecular and cellular biology
  • Place of Presentation: Taos, NM, USA
  • Year and Date: 2009-03-02
• [Presentation] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot in therapy-related leukemia (2009)
  • Author(s): Tashiro S
  • Organizer: The 3rd International Open Laboratory Workshop
  • Place of Presentation: Chiba
  • Year and Date: 2009-02-27
• [Presentation] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint (2009)
  • Author(s): Satoshi Tashiro
  • Organizer: The 3rd International Open Laboratory Workshop
  • Place of Presentation: 千葉市
  • Year and Date: 2009-02-27
• [Presentation] Roland Kanaar and Satoshi Tashiro: ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot in therapy-related leukemia (2009)
  • Author(s): Jiying Sun, Yukako Oma, Masahiko Harata, Kazuteru Kono, Hiroki Shima, Aiko Kinomura, Tsuyoshi Ikura, Shuki Mizutani
  • Organizer: Keystone symposia on molecular and cellular biology
  • Place of Presentation: Taos, USA
• [Presentation] 放射線誘発核内ドメインのダイナミクス (2008)
  • Author(s): 田代聡
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 放射線誘発核内ドメインのダイナミクス (2008)
  • Author(s): 田代聡
  • Organizer: 第31回日本分子生物学会年会・シンポジウム
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-12
• [Presentation] Mechanism of chromosomal translocation involving the MLL gene (2008)
  • Author(s): Jiying Sun
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-11
• [Presentation] RAD51核内ドメイン形成制御機構の解析 (2008)
  • Author(s): 島弘季
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-11
• [Presentation] DNA損傷応答におけるヒストンコードの解明 (2008)
  • Author(s): 井倉毅
  • Organizer: 第31回日本分子生物学会年会・シンポジウム
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-09
• [Presentation] クロマチン幸司構造によるクロマチン間領域形成の制御 (2008)
  • Author(s): 河野一輝
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-09
• [Presentation] 紫外線レーザーマイクロ照射法を用いたゲノム修復の動的制御機構の解析 (2008)
  • Author(s): 田代聡
  • Organizer: 第51回日本放射線影響学会
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-20
• [Presentation] Dynamics of higher order nuclear architecture upon DNA damage (2008)
  • Author(s): Satoshi Tashiro
  • Organizer: The 7th Japan-France Workshop on Radiation Biology
  • Place of Presentation: 千葉市
  • Year and Date: 2008-10-15
• [Presentation] Distribution of non-chromatin nucler domains in interchromatin compartment (2008)
  • Author(s): Satoshi Tashiro
  • Organizer: Cold Spring Harbor Laboratory meeting
  • Place of Presentation: New York, USA
  • Year and Date: 2008-09-19
• [Presentation] 酸化ストレス応答の細胞核ダイナミクス (2008)
  • Author(s): 田代聡
  • Organizer: 第49回原子爆弾後障害研究会・シンポジウム
  • Place of Presentation: 長崎
  • Year and Date: 2008-06-08
• [Presentation] Mechanism of chromosomal translocation involving the MLL gene (2008)
  • Author(s): Satoshi Tashiro
  • Organizer: Ataxia-Telangiectasia Workshop
  • Place of Presentation: 大津市
  • Year and Date: 2008-04-24
• [Presentation] EAD51核内ドメインの多様性 (2008)
  • Author(s): 田代 聡
  • Organizer: 第25回染色体ワークショップ
  • Place of Presentation: 熱海
  • Year and Date: 2008-01-31
• [Presentation] ユビキチン化によって制御されるヒストンH2AXのダイナミクス (2008)
  • Author(s): 井倉 毅
  • Organizer: 第25回染色体ワークショップ
  • Place of Presentation: 熱海
  • Year and Date: 2008-01-31
• [Presentation] ゲノム修復の細胞核ダイナミクス (2008)
  • Author(s): 田代聡
  • Organizer: 「特定領域研究」2領域合同公開シンポジウム「クロマチンシグナリングの分子機構」
  • Place of Presentation: 東京
  • Year and Date: 2008-01-08
• [Presentation] ゲノム修復の細胞核ダイナミクス (2008)
  • Author(s): 田代 聡
  • Organizer: 「特定領域研究」2領域合同 公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-01-08
• [Presentation] Dynamics of higher order nuclear architecture upon DNA damage (2008)
  • Author(s): Satoshi Tashiro
  • Organizer: The 7th Japan-France Workshop on Radiation Biology
  • Place of Presentation: Chiba
• [Presentation] DISTRIBUTION OF NON-CHROMATIN NUCLEAR DOMAINS IN INTERCHROMATIN COMPARTMENT (2008)
  • Author(s): Kazuteru Kono, Thomas Cremer, Satoshi Tashiro
  • Organizer: COMPARTMENT.Cold Spring Harbor Laboratory meeting
  • Place of Presentation: New York, USA
• [Presentation] Mechanism of chromosomal translocation involving the MLL gene (2008)
  • Author(s): JIYING SUN, Satoshi Tashiro
  • Organizer: Ataxia-Telangiectasia Workshop
  • Place of Presentation: 大津
• [Presentation] ュビキチン化によって制御されるヒストンH2AXのダイナミクス (2007)
  • Author(s): 井倉 毅
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Mechanism of chromosomal translocation involving the Mll gene (2007)
  • Author(s): 孫 継英
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] クロマチン間領域における非クロマチン核内ドメイン配置の解析 (2007)
  • Author(s): 河野 一輝
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] DNA損傷応答におけるヒストンH2AZダイナミクスのin vivo解析 (2007)
  • Author(s): 島 弘季
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] RAD51核内ドメイン形成機構の解明 (2007)
  • Author(s): 田代 聡
  • Organizer: 第4回「細胞核ダイナミクス」班会議
  • Place of Presentation: 湯沢
  • Year and Date: 2007-07-02
• [Presentation] Dynamics of BACH2 nuclear foci regualted by SUMOylation (2007)
  • Author(s): Tashiro S
  • Organizer: International Symposium on Functional Organization of the Nucleus
  • Place of Presentation: Awaji
  • Year and Date: 2007-01-09
• [Presentation] Ikura T: In vivo analysis of DNA damage response using microirradiation and living cell imaging techniques (2006)
  • Author(s): Tashiro S
  • Organizer: 日本分子生物学会2006 フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-08
• [Presentation] Dynamics of higher order nuclear architecture upon DNA damage (2006)
  • Author(s): Tashiro S, Shima H, Suzuki H, Sun J
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-23
• [Presentation] Dynamics of DNA damage induced nuclear domains after laser-UVA-microirradiation (2006)
  • Author(s): Tashiro S, Joachim W, Cremer T, Suzuki H, Shima H
  • Organizer: International Symposium Optical analysis of Biomolecular Machines
  • Place of Presentation: Berlin, Germany
• [Presentation] Characterization of Interchromatin Compartment (2005)
  • Author(s): Tashiro, S.
  • Organizer: International Symposium on Ran and Cell Cycle
  • Place of Presentation: Awaji
• [Presentation] Dynamic organization of nuclear domains in DNA damage response (2004)
  • Author(s): Satoshi Tashiro
  • Organizer: The 21st Radiation Biology Center International Symposium on Chromatin and Epigenetic Memory in Damage Response
  • Place of Presentation: Kyoto
• [Presentation] Repression of PML nuclear body-associated transcription by oxidative stress-activated Bach2. (2004)
  • Author(s): Satoshi Tashiro, Akihiko Muto, Keiji Tanimoto, Haruka Tsuchiya, Hiroshi Suzuki, Hideto Hoshino, Minoru Yoshida, Joachim Walter, and Kazuhiko Igarashi
  • Organizer: 46th SYMPOSIUM OF THE SOCIETY FOR HISTOCHEMISTRY
  • Place of Presentation: Praque, Czech Republic
• [Book] Nuclear Dynamics. (Nagata K, Takeyasu K, ed.) (2007)
  • Author(s): Tashiro S, Cremer M, Solovei I, Cremer T. Nuclear Architecture
  • Publisher: Cremer M, Solovei I, Cremer T. Nuclear Architecture: Topology and Function of Chromatin- and Non-Chromatin Nuclear domains
• [Book] Nuclear architecture : ropology and function of chromatin-and non-chroamtin nuclear domains. In "Nuclear dynamics : Approaches from Biochemistry,molecular Cell biology and visual Biology". (2006)
  • Author(s): Satoshi Tashiro, et al.
  • Total Pages: 30
  • Publisher: Springer Inc.
• [Book] Nuclear architecture : topology and function of chromatin- and non-chroamtin nuclear domains. In "Nuclear dynamics : Approaches from Biochemistry, molecular Cell biology and visual Biology". (2006)
  • Author(s): Tashiro S, et al.
  • Publisher: Springer Inc.(In press)
• [Book] 分子標的療法薬imatinibによる慢性骨髄性白血病治療とその問題点 (2005)
  • Author(s): 小野厚、五十嵐和彦、田代聡
  • Publisher: 宇宙堂八木書店
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/cellbio/Site/18ECC511-D377-11DA-83B6-000A956E89D4.html
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/cellbio/Site/18ECC511-D377-11DA-83B6-000A956E89D4.html
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/cellbio/Site/18ECC511-D377-11DA-83B6-000A956E89D4.html