Functions and molecular architechture of the nuclear speckles : roles in the pre-mRNA splicing and nuclear mRNA export

• Project/Area Number: 16084206
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kumamoto University
• Principal Investigator: TANI Tokio Kumamoto University, 大学院・自然科学研究科, 教授 (80197516)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥64,500,000 (Direct Cost: ¥64,500,000); Fiscal Year 2008: ¥12,400,000 (Direct Cost: ¥12,400,000); Fiscal Year 2007: ¥12,400,000 (Direct Cost: ¥12,400,000); Fiscal Year 2006: ¥12,400,000 (Direct Cost: ¥12,400,000); Fiscal Year 2005: ¥12,400,000 (Direct Cost: ¥12,400,000); Fiscal Year 2004: ¥14,900,000 (Direct Cost: ¥14,900,000)
• Keywords: 核スペックル / mRNA / 選択的スプライシング / 低分子化合物 / 蛍光イメージング / 放線菌 / スプライシング因子 / 阻害化合物 / 放線菌培養上清 / バイオロジカルプローブ / SF2 / poly A^+RNA / カハールボディ / 核内低分子RNA / ダイナミクス / 遺伝子 / クローニング / 核外輸送 / snRNA

Research Abstract

核スペックルはmRNA前駆体のスプライシングやmRNA核外輸送など,遺伝子発現と密接な関連をもつ核内構造体である.本研究では,核スペックルの機能と形成機構を明らかにするため,mRNA核外輸送の新規可視化解析系構築,及び核スペックルの形成阻害化合物のスクリーニングと解析などを進め,新規核内構造体TIDRの発見,核スペックルの形成と機能に影響を与える10種類の低分子化合物の同定など多くの研究成果を得た.

Research Products

• [Journal Article] Monitoring of mRNA export (2008)
  • Author(s): Kazuaki Tokunaga and Tokio Tani
  • Journal Title: Current Protocols in Cell Biology 22 Pages: 1-20
  • Peer Reviewed
• [Journal Article] S1-1 nuclear domains : characterization and dynamics as a function of transcriptional activity (2008)
  • Author(s): Akira Inoue, Katsuji Tsugawa, Kazuaki Tokunaga, Kenichi P. Takahashi, Shigehiko Uni, Masatsugu Kimura, Koji Nishio, Naoki Yamamoto, Ken-ichi Honda, Takanori Watanabe, Hideo Yamane and Tokio Tani
  • Journal Title: Biol. Cell 100 Pages: 523-535
  • Peer Reviewed
• [Journal Article] The dynamics of pre-mRNAs and poly (A)+ RNA at speckles in living cells revealed by iFRAP studies (2008)
  • Author(s): Yo Ishihama, Hisashi Tadakuma, Tokio Tani and Takashi Funatsu
  • Journal Title: Exp. Cell Res. 314 Pages: 748-762
  • Peer Reviewed
• [Journal Article] Dss1 associating with the proteasome functions in selective nuclear mRNA export in yeast (2008)
  • Author(s): Taro Mannen, Tomoko Andoh and Tokio Tani
  • Journal Title: Biochem. Biophys. Res. Commun. 365 Pages: 664-671
  • Peer Reviewed
• [Journal Article] Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast (2008)
  • Author(s): Chor-Wai Yo, Daisuke Kaida, Shinichi Nsihimura, Akihisa Matsuyama, Yoko Yashiroda, Hiroshi Taoka, Ken Ishigami, Sidenori Watanabe, Hidenori Nakajima, Tokio Tani, Sueharu Horinouchi and Minoru Yoshida
  • Journal Title: Biochem. Biophys. Res. Commun. 364 Pages: 573-577
  • Peer Reviewed
• [Journal Article] Monitoring of mRNA export (2008)
  • Author(s): Kazuaki Tokunaga
  • Journal Title: Current Protocols in Cell Biology 3
  • Peer Reviewed
• [Journal Article] S1-1 nuclear domains : characterization and dynamics as a functi on of transcriptional activity (2008)
  • Author(s): Akira Inoue
  • Journal Title: Biology of the cell 100 Pages: 523-535
  • Peer Reviewed
• [Journal Article] The dynamics of pre-mRNAs and poly (A)^+ RNA at speckles in living cells revealed by iFRAP studies (2008)
  • Author(s): Ishihama, Y.
  • Journal Title: Experimental Cell Research 314 Pages: 748-762
  • Peer Reviewed
• [Journal Article] Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA (2007)
  • Author(s): Daisuke Kaida, Hajime Motoyoshi, Etsu Tashiro, Takayuki Nojima, Masatoshi Hagiwara, Ken Ishigami, Hidenori Watanabe, Takeshi Kitahara, Tatsuhiko Yoshida, Hidenori Nakajima, Tokio Tani, Sueharu Horinouchi and Minoru Yoshida
  • Journal Title: Nature Chem. Biol. 3 Pages: 576-583
  • Peer Reviewed
• [Journal Article] Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe (2007)
  • Author(s): Noriko Haraguchi, Tomoko Andoh, David Frendewey and Tokio Tani
  • Journal Title: J. Biol. Chem. 282 Pages: 2221-2228
  • Peer Reviewed
• [Journal Article] Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast (2007)
  • Author(s): Fumitaka Mizuki, Takeshi. Namiki, Hiroshi Sato, Hiromi Furukawa, Tadao Matsusaka, Yasumi Ohshima, Ryoko Ishibashi, Tomoko Andoh and Tokio Tani
  • Journal Title: Genes to Cells 12 Pages: 35-47
  • Peer Reviewed
• [Journal Article] 酵母研究におけるアイソトープの活用,「RIを使用した実験法」 (2007)
  • Author(s): 水城史貴,萬年太郎,谷時雄
  • Journal Title: 細胞工学 26 Pages: 92-97
• [Journal Article] Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA (2007)
  • Author(s): Kaida, D.
  • Journal Title: Nature Chemical Biology 3 Pages: 576-583
  • Peer Reviewed
• [Journal Article] Dssl associating with the proteasome functions in selective nuclear mRNA export in yeast (2007)
  • Author(s): Mannen, T.
  • Journal Title: Biochemical and Biophysical Research Communication 365 Pages: 664-671
  • Peer Reviewed
• [Journal Article] Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast (2007)
  • Author(s): Yo, C. W.
  • Journal Title: Biochemical and Biophysical Research Communication 364 Pages: 573-577
  • Peer Reviewed
• [Journal Article] Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast (2007)
  • Author(s): Mizuki, F.
  • Journal Title: Genes to Cells 12 Pages: 35-47
• [Journal Article] Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe (2007)
  • Author(s): Haraguchi, N.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 2221-2228
• [Journal Article] Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip (2006)
  • Author(s): Tomoko Andoh, Yukiko Oshiro, Sachiko Hayashi, Hideki Takeo and Tokio Tani
  • Journal Title: Biochem. Biophys. Res. Commun. 351 Pages: 999-1004
  • Peer Reviewed
• [Journal Article] Imaging of single mRNA molecules movement within a living cell nucleus (2006)
  • Author(s): Hisashi Tadakuma, Yo Ishihama, Toshiharu Shibuya, Tokio Tani and Takashi Funatsu
  • Journal Title: Biochem. Biophys. Res. Commun. 344 Pages: 772-779
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells : Nuclear mRNA export is coupled to ongoing gene transcription (2006)
  • Author(s): Kazuaki Tokunaga, Toshiharu Shibuya, Yo Ishihama, Hisashi Tadakuma, Miyuki Ide, Minoru Yoshida, Takashi Funatsu, Yasumi Ohshima and Tokio Tani
  • Journal Title: Genes to Cells 11 Pages: 305-317
  • Peer Reviewed
• [Journal Article] mRNA核外輸送の蛍光イメージングと核内構造 (2006)
  • Author(s): 谷時雄
  • Journal Title: ファルマシア 51 Pages: 2197-2199
• [Journal Article] RNA蛍光イメージングで拓く細胞核研究のフロンティア (2006)
  • Author(s): 船津高志,谷時雄
  • Journal Title: Pharma Vision News Vision News 7 Pages: 23-29
• [Journal Article] Imaging of single mRNA molecules movement within a living cell nucleus (2006)
  • Author(s): Tadakuma, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 344 Pages: 772-779
• [Journal Article] Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip (2006)
  • Author(s): Andoh, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 351 Pages: 999-1004
• [Journal Article] Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells : nuclear mRNA export is coupled to ongoing gene transcription (2006)
  • Author(s): Tokunaga, K.
  • Journal Title: Genes to Cells 11 Pages: 305-317
• [Journal Article] Single molecule imaging of mRNA splicing (2005)
  • Author(s): Yo Ishihama, Hisashi Tadakuma, Tokio Tani and Takashi Funatsu
  • Journal Title: Nucleic Acids Symp. Ser. 49 Pages: 209-210
• [Journal Article] Hsp16p is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe (2005)
  • Author(s): Jun-ichi Yoshida and Tokio Tani
  • Journal Title: Cell Structure and Function 29 Pages: 125-138
  • Peer Reviewed
• [Journal Article] Hsp16 is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe (2005)
  • Author(s): Yoshida, J.
  • Journal Title: Cell Structure and Function 29 Pages: 125-138
• [Journal Article] Hsp16p is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe (2005)
  • Author(s): Jun-ichi Yoshida
  • Journal Title: Cell Structure and Function (印刷中)
• [Journal Article] The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase (2004)
  • Author(s): Tomoko Andoh, Abul Kalam Azad, Asako Shigematsu, Yasumi Ohshima and Tokio Tani
  • Journal Title: Biochem. Biophys. Res. Commun. 317 Pages: 1138-1143
  • Peer Reviewed
• [Journal Article] The nucleolus is involved in mRNA export from the nucleus in fission yeast (2004)
  • Author(s): Takashi Ideue, Abul Kalam Azad, Jun-ichi Yoshida, Tadao Matsusaka, Mitsuhiro Yanagida, Yasumi Ohshima and Tokio Tani
  • Journal Title: J. Cell Sci. 117 Pages: 2887-2895
  • Peer Reviewed
• [Journal Article] イーストトラフィック:mRNA細胞内輸送のしくみと制御 (2004)
  • Author(s): 谷時雄,安東知子
  • Journal Title: 実験医学 22 Pages: 2447-2453
• [Journal Article] The fission yeast ptr1^+ gene involved in nuclear mRNA export encodes a putative uhiquitin ligase (2004)
  • Author(s): Tomoko Andoh
  • Journal Title: Biochemical and Biophysical Research Communication 317 Pages: 1138-1143
• [Journal Article] The nucleolus is involved in mRNA export from the nucleus in fission veast (2004)
  • Author(s): Takashi Ideue
  • Journal Title: J.Cell Science 117 Pages: 2887-2895
• [Journal Article] イーストトラフィック:mRNA細胞内輸送のしくみと制御 (2004)
  • Author(s): 谷時雄
  • Journal Title: 実験医学 22 Pages: 2447-2453
• [Presentation] poly(A)?RNAの核スペックル局在化を阻害する天然化合物のスクリーニング (2008)
  • Author(s): 松尾陽太,東祐子,志柿花矢,豊田修吉,徳永和明,五十嵐雅之,谷時雄
  • Organizer: 第31回日本分子生物学会・第81回日本生化学学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 核スペックルの形成とRNAダイナミクス (2008)
  • Author(s): 志柿花矢
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] poly(A)^+RNAの核スペックル局在化を阻害する天然化合物のスクリーニング (2008)
  • Author(s): 松尾陽太
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] スプライシング因子SF2の核スペックル局在化阻害化合物のスクリーニング (2008)
  • Author(s): 江藤俊志
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Poly(A)+RNAの細胞内動態に影響を与える化合物のスクリーニング (2008)
  • Author(s): 豊田修吉,東祐子,徳永和明,松尾陽太,志柿花矢,五十嵐雅之,谷時雄
  • Organizer: 第10回RNAミーティングs
  • Place of Presentation: 札幌市
  • Year and Date: 2008-07-23
• [Presentation] Poly(A)+RNAの細胞内動態に影響を与える化合物のスクリーニング (2008)
  • Author(s): 豊田修吉
  • Organizer: 第10回RNAミーティング
  • Place of Presentation: 札幌市
  • Year and Date: 2008-07-23
• [Presentation] Screening for natural compounds that affect formation of nuclear speckles (2008)
  • Author(s): Kaya Shigaki
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Presentation] Screening for natural compounds that affect formation of nuclear speckle (2008)
  • Author(s): Kaya Shigaki,Kazuaki Tokunaga,Takashi Eto,Yutaka Kido,Masayuki Igarashi and Tokio Tani
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 核スペックルの形成とRNAダイナミクス (2008)
  • Author(s): 志柿花矢,豊田修吉,江藤俊志,松尾陽太,三原由揮,徳永和明,五十嵐雅之,谷時雄
  • Organizer: 第31回日本分子生物学会・第81回日本生化学学会合同大会
  • Place of Presentation: 神戸
• [Presentation] スプライシング因子SF2の核スペックル局在化阻害化合物のスクリーニング (2008)
  • Author(s): 江藤俊志,志柿花矢,三原由揮,徳永和明,五十嵐雅之,谷時雄
  • Organizer: 第31回日本分子生物学会・第81回日本生化学学会合同大会
  • Place of Presentation: 神戸
• [Presentation] 核スペックル形成阻害化合物のスクリーニング (2007)
  • Author(s): 徳永和明
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] The dynamics of pre-mRNAs at speckles in living cells revealed by iFRAP studies (2007)
  • Author(s): 石浜陽
  • Organizer: 第59回日本細胞生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] The fission yeast ptr5^+ gene required for nuclear mRNA export encodes the nucleoporins Nup85p (2007)
  • Author(s): 渡邉常義
  • Organizer: 第59回日本細胞生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] The membrane-bound protein Ptr10p participates in mRNA export through Rae1p in Schizosaccharomyces pombe (2007)
  • Author(s): 宮部麻耶子
  • Organizer: 第59回日本細胞生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] The dynamics of pre-mRNAs at speckles in living cells revealed by iFRAP studies (2007)
  • Author(s): Yo Ishihama,Hisashi Tadakuma,TokioTani,Takashi Funatsu
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会(JSDB-JSCB 2007 合同大会)
  • Place of Presentation: 福岡
• [Presentation] 核スペックル形成阻害化合物のスクリーニング (2007)
  • Author(s): 徳永和明,志柿花矢,城戸裕,五十嵐雅之,安東知子,谷時雄
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 核から細胞質へのmRNA輸送:分子機構の解明と疾患 (2006)
  • Author(s): 谷時雄
  • Organizer: 17thフォーラム・イン・ドージン
  • Place of Presentation: 熊本
  • Year and Date: 2006-11-17
• [Presentation] 核から細胞質へのmRNA輸送:転写と輸送のクロストーク (2006)
  • Author(s): 谷時雄
  • Organizer: リエゾンラボ研究会
  • Place of Presentation: 熊本
  • Year and Date: 2006-04-19
• [Presentation] NUCLEAR EXPORT OF mRNA IN FISSION YEAST (2006)
  • Author(s): Fumitaka Mizuki,Ryoko Ishibashi,Kiriko Matsushita,Mayako Miyabe,Nobuyoshi Watanabe,Tomoko Andoh and Tokio Tani
  • Organizer: Functional organization of the nucleus
  • Place of Presentation: 淡路夢舞台
• [Presentation] S1-1 nuclear bodyとその機能 (2005)
  • Author(s): 井上晃,西尾康二,高橋研一,津川克治,山元直樹,八谷和孝,山根英雄,木村政継,徳永和明,谷時雄
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-07
• [Presentation] 蛍光イメージングによるmRNA核外輸送機構の解析:mRNAの核から細胞質への輸送と転写のカップリング (2004)
  • Author(s): 徳永和明,渋谷利治,井手深雪,谷時雄
  • Organizer: 核ダイナミクス研究会
  • Place of Presentation: 倉敷
• [Presentation] 転写不活化に伴う新規核内構造体TIDRへのmRNA蓄積:mRNAの核外輸送と転写反応のカップリング (2004)
  • Author(s): 徳永和明,渋谷利治,井手深雪,安東知子,谷時雄
  • Organizer: 第6回RNAミーティング
  • Place of Presentation: 熊本
• [Presentation] 遺伝子の転写とmRNA核外輸送の連携:転写不活性化によりmRNAは核内ドメインTIDR に蓄積する (2004)
  • Author(s): 徳永和明,井手深雪,谷時雄
  • Organizer: 第27回日本分子生物学会
  • Place of Presentation: 神戸
• [Book] スプライシングとmRNA核外輸送,「酵母のすべて」 (2007)
  • Author(s): 水城史貴,谷時雄
  • Publisher: シュプリンガーフェアラーク東京
• [Book] スプライシング,生物物理学ハンドブック (2007)
  • Author(s): 谷時雄
  • Publisher: 朝倉書店
• [Book] 酵母研究-in vivoパルス標識によるRNAの細胞内動態解析法-,細胞工学別冊「RIの逆襲」 (2007)
  • Author(s): 水城史貴,萬年太郎,谷時雄
  • Publisher: 秀潤社
• [Remarks] ホームページ等
  • URL: http://aster.sci.kumamoto-u.ac.jp/~biohome/staff/tani/index.htm
• [Remarks]
  • URL: http://aster.sci.kumamoto-u.ac.jp/~biohome/staff/tani/index.htm