Functions and molecular architechture of the nuclear speckles : roles in the pre-mRNA splicing and nuclear mRNA export
| Project/Area Number |
16084206
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kumamoto University |
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Principal Investigator |
TANI Tokio Kumamoto University, 大学院・自然科学研究科, 教授 (80197516)
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| Project Period (FY) |
2004 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥64,500,000 (Direct Cost: ¥64,500,000)
Fiscal Year 2008: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2007: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2006: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2005: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2004: ¥14,900,000 (Direct Cost: ¥14,900,000)
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| Keywords | 核スペックル / mRNA / 選択的スプライシング / 低分子化合物 / 蛍光イメージング / 放線菌 / スプライシング因子 / 阻害化合物 / 放線菌培養上清 / バイオロジカルプローブ / SF2 / poly A^+RNA / カハールボディ / 核内低分子RNA / ダイナミクス / 遺伝子 / クローニング / 核外輸送 / snRNA |
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| Research Abstract |
核スペックルはmRNA前駆体のスプライシングやmRNA核外輸送など,遺伝子発現と密接な関連をもつ核内構造体である.本研究では,核スペックルの機能と形成機構を明らかにするため,mRNA核外輸送の新規可視化解析系構築,及び核スペックルの形成阻害化合物のスクリーニングと解析などを進め,新規核内構造体TIDRの発見,核スペックルの形成と機能に影響を与える10種類の低分子化合物の同定など多くの研究成果を得た.
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Report
(6 results)
Research Products
(63 results)
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[Journal Article] S1-1 nuclear domains : characterization and dynamics as a function of transcriptional activity2008
Author(s)
Akira Inoue, Katsuji Tsugawa, Kazuaki Tokunaga, Kenichi P. Takahashi, Shigehiko Uni, Masatsugu Kimura, Koji Nishio, Naoki Yamamoto, Ken-ichi Honda, Takanori Watanabe, Hideo Yamane and Tokio Tani
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Journal Title
Biol. Cell 100
Pages: 523-535
Related Report
Peer Reviewed
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[Journal Article] Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast2008
Author(s)
Chor-Wai Yo, Daisuke Kaida, Shinichi Nsihimura, Akihisa Matsuyama, Yoko Yashiroda, Hiroshi Taoka, Ken Ishigami, Sidenori Watanabe, Hidenori Nakajima, Tokio Tani, Sueharu Horinouchi and Minoru Yoshida
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Journal Title
Biochem. Biophys. Res. Commun. 364
Pages: 573-577
Related Report
Peer Reviewed
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[Journal Article] Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA2007
Author(s)
Daisuke Kaida, Hajime Motoyoshi, Etsu Tashiro, Takayuki Nojima, Masatoshi Hagiwara, Ken Ishigami, Hidenori Watanabe, Takeshi Kitahara, Tatsuhiko Yoshida, Hidenori Nakajima, Tokio Tani, Sueharu Horinouchi and Minoru Yoshida
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Journal Title
Nature Chem. Biol. 3
Pages: 576-583
Related Report
Peer Reviewed
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