Integration of nitrogen signaling and nitrogen metabolisms in rice

• Project/Area Number: 16085201
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: YAMAYA Tomoyuki Tohoku University, 大学院農学研究科, 教授 (30144778)
• Co-Investigator(Kenkyū-buntansha): 早川 俊彦 東北大学, 大学院・農学研究科, 准教授 (60261492)
• Co-Investigator(Renkei-kenkyūsha): HAYAKAWA Toshihiko 東北大学, 大学院農学研究科, 准教授 (60261492)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥79,400,000 (Direct Cost: ¥79,400,000); Fiscal Year 2008: ¥18,800,000 (Direct Cost: ¥18,800,000); Fiscal Year 2007: ¥20,500,000 (Direct Cost: ¥20,500,000); Fiscal Year 2006: ¥20,500,000 (Direct Cost: ¥20,500,000); Fiscal Year 2005: ¥9,800,000 (Direct Cost: ¥9,800,000); Fiscal Year 2004: ¥9,800,000 (Direct Cost: ¥9,800,000)
• Keywords: オルガネラ / 分化 / 環境適応 / 代謝性 / 高次機能 / 植物分子 / イネ / グルタミン情報伝達 / 窒素流 / プラスチド機能 / 高次機能発 / グルタミン合成酵素 / メタボローム / トランスクリプトーム / 窒素転流 / 遺伝子破壊 / QTL解析

Research Abstract

イネの窒素転流は、成育や生産性に直接関わる。老化器官からの転流と、若い器官での再利用に関わる高次機能に焦点をあて、グルタミン情報伝達系の分子実体の解明と、窒素利用効率向上戦略に関わる高次機能発現の分子基盤を確立した。グルタミン情報受容体候補分子の特性、GS1;1 遺伝子破壊変異体のメタボローム解析、根の伸長を促進するQTL などから、窒素利用におけるイネの高次機能の発現に関する理解が格段に深まった。

Research Products

• [Journal Article] Cytosolic glutamine synthetase is present in phloem sap from rice (Oryza sativa L.) (2009)
  • Author(s): Tanaka, T., Suzui, N., Hayashi, H., Yamaya, T. and Yoneyama, T
  • Journal Title: Soil Sci. Plant Nutr 55(1) Pages: 102-106
  • NAID: 110007502712
  • Peer Reviewed
• [Journal Article] Cytosolic glutamine synthetase is present in phloem sap from rice (Oryza sativa L.) (2009)
  • Author(s): Tanaka, et.al.
  • Journal Title: Soil Science Plant Nutrition 55 Pages: 102-106
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.) (2008)
  • Author(s): Kudo, T., Kawai, A., Yamaya, T. and Hayakawa, T
  • Journal Title: Physiol. Plant 133(2) Pages: 167-179
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, H., Ueda, N., Kojima, M., Makita, N., Yamaya, T. and H. Sakakibara
  • Journal Title: Proc. Natl. Acad. Sci. USA 105(7) Pages: 2734-2739
  • NAID: 130006995125
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, et.al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 2734-2739
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.) (2008)
  • Author(s): Kudo, et.al.
  • Journal Title: Physiologia Plantaram 133 Pages: 169-179
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, et. al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 2734-2739
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.) (2008)
  • Author(s): Kudo, et. al.
  • Journal Title: Physiologia Plantaram (In press)
  • Peer Reviewed
• [Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene (2007)
  • Author(s): Suzuki, Y., Ohkubo, M., Hatakeyama, H., Ohashi, K., Yoshizawa, R., Kojima, S., Hayakawa, T., Yamaya, T., Mae, T. and Makino, A
  • Journal Title: Plant Cell Physiol 48(4) Pages: 626-637
  • NAID: 10027001603
  • Peer Reviewed
• [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L.) (2007)
  • Author(s): Tabuchi, M., Abiko, T. and Yamaya, T
  • Journal Title: J. Exp. Bot 58(9) Pages: 2319-2327
  • Peer Reviewed
• [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L.) (2007)
  • Author(s): Tabuchi, et. al.
  • Journal Title: Journal of Experimental Botany 58 Pages: 2319-2327
  • Peer Reviewed
• [Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene (2007)
  • Author(s): Suzuki, et. al.
  • Journal Title: Plant Cell Physiology 48 Pages: 626-637
  • Peer Reviewed
• [Journal Article] Atomic structure of plant glutamine synthetase : a key enzyme for plant productivity (2006)
  • Author(s): Unno, H., Uchida, T., Sugawara, H., Kurisu, G., Sugiyama, T., Yamaya, T., Sakakibara, H., Hase, T. and Kusunoki, M
  • Journal Title: J. Biol. Chem 281 Pages: 29287-29296
  • Peer Reviewed
• [Journal Article] ACT-domain repeat protein 7, ACR7, interacts with a chaperon HSP18.0-CII in rice nuclei (2006)
  • Author(s): Hayakawa, T., Kudo, T., Ito, T., Takahashi, N. and Yamaya, T
  • Journal Title: Plant Cell Physiol 47 Pages: 891-904
  • NAID: 10018185447
  • Peer Reviewed
• [Journal Article] Epistatic interaction of QTLs controlling leaf bronzing in rice (Oryza sativa L.) grown in a saline paddy field (2006)
  • Author(s): Takehisa, H., Ueda, T., Fukuta, Y., Obara, M., Abe, T., Yano, M., Yamaya, T., Kameya, T., Higashitani, A. and Sato, T
  • Journal Title: Breeding Sci 56(3) Pages: 287-293
  • NAID: 110004789109
  • Peer Reviewed
• [Journal Article] Gln-49 and Ser-174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase (2006)
  • Author(s): Ishiyama, K., Inoue, E., Yamaya, T. and Takahashi, H
  • Journal Title: Plant Cell Physiol 47(2) Pages: 299-303
  • NAID: 10017272908
  • Peer Reviewed
• [Journal Article] Gln-49 and Ser-174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase (2006)
  • Author(s): Ishiyama et al.
  • Journal Title: Plant Cell Physiology 47(2) Pages: 299-303
• [Journal Article] Epistatic interaction of QTLs controlling leaf bronzing in rice (Oryza sativa L.) grown in a saline paddy field (2006)
  • Author(s): Takehisa et al.
  • Journal Title: Breeding Sci. 56(3) Pages: 287-293
• [Journal Article] ACT-domain repeat protein 7, ACR7, interacts with a chaperon HSP18.0-CII in rice nuclei (2006)
  • Author(s): Hayakawa et al.
  • Journal Title: Plant Cell Physiology 47(7) Pages: 891-904
• [Journal Article] Atomic structure of plant glutamine synthetase : a key enzyme for plant productivity (2006)
  • Author(s): Unno et al.
  • Journal Title: J. Biol. Chem. 281 Pages: 29287-2929
• [Journal Article] Gln-49 and Ser-174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase (2006)
  • Author(s): Ishiyama et al.
  • Journal Title: Plant Cell Physiology (印刷中)
• [Journal Article] Differential distribution of proteins expressed in companion cells in the sieve element-companion cell complex of rice plants (2005)
  • Author(s): Fukuta, A., Fujimaki, S., Mori, T., Suzui, N., Ishiyama, K., Hayakawa, T., Yamaya, T., Fujiwara, T., Yoneyama, T. and Hayashi, H
  • Journal Title: Plant Cell Physiol 46(11) Pages: 1779-1786
  • NAID: 10016887252
  • Peer Reviewed
• [Journal Article] Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice : Candidate for providing carbon skeletons to NADH-glutamate synthase (2005)
  • Author(s): Abiko, T., Obara, M., Ushioda, A., Hayakawa, T., Hodges, M. and Yamaya, T
  • Journal Title: Plant Cell Physiol 46 Pages: 1724-1734
  • Peer Reviewed
• [Journal Article] Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1 ; 1, a cytosolic glutamine synthetase1;1 (2005)
  • Author(s): Tabuchi, M., Sugiyama, K., Ishiyama, K., Inoue, E., Sato, T., Takahashi, H. and Yamaya, T
  • Journal Title: Plant J 42(5) Pages: 641-651
  • Peer Reviewed
• [Journal Article] Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase 1;1 (2005)
  • Author(s): Tabuchi et al.
  • Journal Title: Plant Journal 42(5) Pages: 641-651
• [Journal Article] Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice : Candidate for providing carbon skeletons to NADH-glutamate synthase (2005)
  • Author(s): Abiko et al.
  • Journal Title: Plant Cell Physiology 46(10) Pages: 1724-1734
• [Journal Article] Differential distribution of proteins expressed in companion cells in the sieve element-companion cell complex of rice plants (2005)
  • Author(s): Fukuta et al.
  • Journal Title: Plant Cell Physiology 46(11) Pages: 1779-1786
• [Journal Article] Sever reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase 1;1 gene (2005)
  • Author(s): Tabuchi.M.et al.
  • Journal Title: Plant Journal (in press)
• [Journal Article] Biochemical backgrounds of compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots (2004)
  • Author(s): Ishiyama, K., Inoue, E., Tabuchi, M., Yamaya, T. and Takahashi, H
  • Journal Title: Plant Cell Physiol 45 (11) Pages: 1640-1647
  • NAID: 10014020704
  • Peer Reviewed
• [Journal Article] Interaction of acetylglutamate kinase with a PII-like protein in rice (2004)
  • Author(s): Sugiyama, K., Hayakawa, T., Kudo, T. Ito, T. and Yamaya, T
  • Journal Title: Plant Cell Physiol 45 Pages: 1768-1778
  • Peer Reviewed
• [Journal Article] Identification and characterization of QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice (Oryza sativa L.) (2004)
  • Author(s): Obara, M., Sato, T., Sasaki, S., Kashiba, K., Nakamura, I., Ebitani, T., Yano, M. and Yamaya, T
  • Journal Title: Theoretical Applied Genetics 110 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetases in Arabidopsis (2004)
  • Author(s): Ishiyama, K., Inoue, E., Watanabe-Takahashi, A., Obara, M., Yamaya, T. and Takahashi, H
  • Journal Title: J. Biol. Chem 279 Pages: 16598-16605
  • Peer Reviewed
• [Journal Article] Interaction of acetylglutamate kinase with a PII-like protein in rice (2004)
  • Author(s): Sugiyama, K. et al.
  • Journal Title: Plant Cell Physiology 45 Pages: 1768-1778
• [Journal Article] Identification and characterization of QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice (Oryza sativa L.) (2004)
  • Author(s): Obara M. et al.
  • Journal Title: Theoretical Applied Genetics 110 Pages: 1-11
• [Presentation] イネのアンモニウムイオン欠乏に対する応答機構 (2009)
  • Author(s): 山谷知行
  • Organizer: 植物ストレス科学研究ネットワーク発足シンポジウム, 「ストレスと戦う植物の戦略と次世代作物の作出」
  • Place of Presentation: 倉敷市芸文館アイシアター
• [Presentation] Genetic and physiological approach of nitrogen utilization toward improving environmental adaptation of rice (2008)
  • Author(s): Obara, M. and Yamaya, T
  • Organizer: Workshop on Development of Environmentally-friendly Water-saving Technologies for Rice
  • Place of Presentation: JIRCAS Conference Room, Tsukuba
  • Year and Date: 2008-06-18
• [Presentation] Molecular mechanisms for plant biomass production - rice as a model plant (2008)
  • Author(s): Yamaya, T
  • Organizer: UCR Tech Horizon 2008 Conference
  • Place of Presentation: UC Riverside, CA, USA
• [Presentation] Molecular mechanisms for plant biomass production-rice as a model plant (2008)
  • Author(s): Yamaya, T
  • Organizer: UCR Tech Horizon 2008 Conference (invited)
  • Place of Presentation: UC Riverside, CA, USA
• [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice (2007)
  • Author(s): Yamaya, T
  • Organizer: Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Lancaster University, UK
• [Presentation] Identification and characterization of quantitative trait loci in nitrogen utilization of rice (2006)
  • Author(s): Obara, M., Tamura, W., Ono, H., Ebitani, T., Yano, M., Sato, T. and Yamaya, T
  • Organizer: The 100th Anniversary of Tohoku University International Symposium : Frontiers in Rice Science - from Gene to Field-
  • Place of Presentation: Sendai International Center, Sendai
• [Presentation] Mechanism of nitrogen remobilization in rice (2006)
  • Author(s): Tabuchi, M., Hayakawa, T. and Yamaya, T
  • Organizer: The 100th Anniversary of Tohoku University International Symposium : Frontiers in Rice Science - from Gene to Field-
  • Place of Presentation: Sendai International Center, Sendai
• [Presentation] Nitrogen utilization in rice : molecular, physiological and genetic analyses (2006)
  • Author(s): Yamaya, T
  • Organizer: Sino-Japanese Workshop on Plant Nutrition
  • Place of Presentation: Yunnan Agricultural University, Kunming, China
• [Presentation] Comprehensive Approaches for Understanding Mechanisms of Nitrogen Utilization in Rice (2006)
  • Author(s): Yamaya, T., Tabuchi, M. and Obara, M
  • Organizer: The 53rd NIBB Conference : Dynamic Organelles in Plants
  • Place of Presentation: NIBB, Okazaki
• [Presentation] イネの窒素の栄養生理と生産性の分子的基盤 (2005)
  • Author(s): 早川俊彦、山谷知行
  • Organizer: 日本土壌肥料学会2005年度島根大会シンポジウム
  • Place of Presentation: 島根大学
  • Year and Date: 2005-09-08
• [Presentation] Nitrogen utilization in rice : From the aspect of molecular biology and molecular genetics (2005)
  • Author(s): Yamaya, T
  • Organizer: Invited Seminar at Crop Performance and Improvement Division, Rothamsted Research
  • Place of Presentation: Harpenden, UK
  • Year and Date: 2005-06-01
• [Presentation] Mechanisms of nitrogen utilization and genetic approaches for improvement of rice yield (2005)
  • Author(s): Yamaya, T., Obara, M., Tabuchi, M. and Sato, T
  • Organizer: The Symposium on Molecular and Cellular Biology of Plant Storage Function - from Gene to Food
  • Place of Presentation: Nagoya Univ
• [Presentation] Glutamine sensor and signaling in rice (2005)
  • Author(s): Hayakawa, T., Sugiyama, K. and Yamaya, T
  • Organizer: Xth France-Japan Workshop on Plant Sciences : Cellular signaling and development
  • Place of Presentation: Toulouse, France
• [Presentation] Characteristics of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1 gene (2005)
  • Author(s): Tabuchi, M. and Yamaya, T
  • Organizer: 6th International Workshop on Plant Sulfur Metabolosm : physiological, molecular biochemical, ecological, environmental, agricultural, nutritional, nutra-pharmaceutical aspects
  • Place of Presentation: Kazusa Akademia Center, Kisarazu, Japan
• [Presentation] イネにおける窒素利用機構と生産性向上, 岩手大学21世紀COEプロジェクト「熱-生命システム相関学拠点創成」 (2004)
  • Author(s): 山谷知行
  • Organizer: 第1回シンポジウム特別講演
  • Place of Presentation: 岩手大学
  • Year and Date: 2004-11-04
• [Presentation] Molecular mechanisms for nitrogen recycling in rice (2004)
  • Author(s): Yamaya, T
  • Organizer: INRA-Seminar
  • Place of Presentation: INRA, Versailles, France
  • Year and Date: 2004-01-13
• [Presentation] イネにおける窒素代謝のメカニズム (2004)
  • Author(s): 山谷知行
  • Organizer: 日本育種学会シンポジウム「植物栄養特性の育種への利用-窒素栄養と遺伝・育種学」
  • Place of Presentation: 三重大学
• [Presentation] イネの窒素利用と穂数を決定している遺伝子座の解析 (2004)
  • Author(s): 小原実広、佐藤雅志、矢野昌裕、山谷知行
  • Organizer: 日本植物学会第68回大会シンポジウム「植物種子における炭素/窒素代謝ネットワークの制御」
  • Place of Presentation: 日大湘南キャンパス
• [Presentation] Chromosome-substituted lines confirmed QTL on chromosome 2 for GS1 protein content and tiller number in rice (2004)
  • Author(s): Yamaya, T., Obara, M., Yano, M., Sato, T
  • Organizer: The 7th International Symposium on Inorganic Nitrogen Assimilation in Plants : from the genome to the agro-ecosystem
  • Place of Presentation: Wageningen, The Netherlands
• [Book] Nitrogen Assimilation in Plants, (Ammonia absorption and assimilation in plants) (2009)
  • Author(s): Kojima, K., von Wiren, N. and Yamaya, T
  • Publisher: Research Signpost, India(in press)
• [Book] 植物栄養学 第2版, (細胞内アンモニア代謝、アンモニアトランスポーター), (間藤徹、馬建鋒、藤原徹 編) (2009)
  • Author(s): 山谷知行
  • Publisher: 文永堂出版 (印刷中)
• [Book] 植物の百科事典, (物質代謝) (2009)
  • Author(s): 山谷知行
  • Publisher: 朝倉書店
• [Book] 植物の百科事典, (窒素代謝) (2009)
  • Author(s): 山谷知行
  • Total Pages: 16
  • Publisher: 朝倉書店
• [Book] 植物栄養学第2版 (2009)
  • Author(s): 山谷知行
  • Publisher: 文永堂出版(印刷中)
• [Book] 2006年度秋田大会記念誌, (水稲の窒素利用に関する分子生物学的アプローチ。東北の農業と土壌肥料), (日本土壌肥料学会東北支部編) (2006)
  • Author(s): 山谷知行
• [Book] イネの生産性・品質と栄養生理, (イネの窒素の栄養生理と生産性の分子的基盤), (日本土壌肥料学会編) (2006)
  • Author(s): 早川俊彦、山谷知行
  • Publisher: 博友社
• [Book] Focus on Plant Molecular Biology-2 : Biotechnological Approaches to Improve Nitrogen Use Efficiency in Plants, (Glutamate syntase, ammonia assimilation and plant productivity), (Eds. By Singh, R.P. and Jaiwal, P.K.) (2006)
  • Author(s): Srivastava, H.S., Shankar, N., Yamaya, T. and Singh, R.P
  • Publisher: Studium Press, LLC, Houston, Texas, U.S.A
• [Book] イネの生産性・品質と栄養生理 (2006)
  • Author(s): 早川俊彦, 山谷知行
  • Total Pages: 168
  • Publisher: 博友社
• [Book] Focus on Plant Molecular Biology-2 : Biotechnological Approaches to Improve Nitrogen Use Efficiency in Plants (Eds.by Singh, R.P.and Jaiwal, P.K.) (2006)
  • Author(s): Srivastava et al.
  • Total Pages: 372
  • Publisher: Studium Press, LLC, Houston, Texas, USA
• [Book] Nitrogen Acquisition and Assimilation in Higher Plants, (Metabolic Regulation of Ammonium Uptake and Assimilation), Plant Ecophysiology Series, (Eds. By Stulen, I. and Amancio, S) (2004)
  • Author(s): Yamaya, T. and Oaks, A
  • Publisher: Kluwer Academic Publisher, The Netherlands
• [Book] 植物ゲノム科学事典, (駒嶺穆, ら編)
  • Author(s): 山谷知行
  • Publisher: 朝倉書店
• [Remarks]
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