Research of genetic network for vacuolar function involved in gravity perception and morphology in Arabidopsis.

• Project/Area Number: 16085205
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: MORITA Miyo Nara Institute of Science and Technology, バイオサイエンス研究科, 准教授 (10314535)
• Co-Investigator(Kenkyū-buntansha): TASAKA Masao 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (90179680)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥86,800,000 (Direct Cost: ¥86,800,000); Fiscal Year 2008: ¥17,400,000 (Direct Cost: ¥17,400,000); Fiscal Year 2007: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2006: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2005: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2004: ¥30,400,000 (Direct Cost: ¥30,400,000)
• Keywords: 植物 / 環境応答 / 重力屈性 / 小胞輸送 / 液胞 / シロイヌナズナ / 遺伝学 / オルガネラ / 細胞骨格

Research Abstract

我々はシロイヌナズナの環境適応戦略の一つである重力屈性において、小胞輸送が支える液胞機能が重力感受に必要であることを見いだした。本研究では、液胞やそれに至る輸送系が、どのようにして重力屈性や形態形成などの高次機能に関与するのか、より具体的に分子レベルで理解することを目的として、主に分子遺伝学的な手法で研究を行った。その結果,複数の新たな小胞輸送関連因子を単離し、その機能の一端を解明した。

Research Products

• [Journal Article] 高等植物における重力感受の分子機構 (2009)
  • Author(s): 中村守貴, 田坂昌生, 森田(寺尾)美代
  • Journal Title: 生物物理 49巻3号 Pages: 116-121
  • NAID: 10025975571
  • Peer Reviewed
• [Journal Article] 高等植物における重力感受の分子機構 (2009)
  • Author(s): 中村守貴
  • Journal Title: 生物物理 49-3(印刷中)
  • Peer Reviewed
• [Journal Article] A genetic framework for the control of cell division and differentiation in the root meristem (2008)
  • Author(s): Dello Ioio R, Nakamura K, Moubayidin L, Perilli S, Taniguchi M, Morita MT, Aoyama T, Costantino P, Sabatini S.
  • Journal Title: Science 322(5906) Pages: 1380-1384
  • Peer Reviewed
• [Journal Article] A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana (2008)
  • Author(s): Ebine, K., Okatani, Y., Uemura, T., Goh, T., Shoda, K., Niihama, M., Morita, M.T., Spitzer, C., Otegui, M.S., Nakano, A., and Ueda, T.
  • Journal Title: Plant Cell 11 Pages: 3006-3021
  • Peer Reviewed
• [Journal Article] 重力屈性から解くメンブレントラフィック (2008)
  • Author(s): 森田(寺尾)美代
  • Journal Title: 蛋白質核酸酵素 53巻16号 Pages: 2308-2312
• [Journal Article] A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana (2008)
  • Author(s): Ebine, K.
  • Journal Title: Plant Cell 20 Pages: 3006-3021
  • Peer Reviewed
• [Journal Article] A genetic framework for the control of cell division and differentiation in the root meristem (2008)
  • Author(s): Dello Ioio R
  • Journal Title: Science 322 Pages: 1380-1384
  • Peer Reviewed
• [Journal Article] 重力屈性から解くメンブレントラフィック (2008)
  • Author(s): 森田(寺尾)美代
  • Journal Title: 蛋白質核酸酵素 53 Pages: 2308-2312
• [Journal Article] endodermal-amyloplast less 1 is a novel allele of SHORT-ROOT (2007)
  • Author(s): Morita, M. T., Saito, C., Nakano, A. and Tasaka, M.
  • Journal Title: Advances in Space Research 39 Pages: 1127-1133
  • Peer Reviewed
• [Journal Article] CRM1/BIG-mediated auxin action regulates Arabidopsis inflorescence development (2007)
  • Author(s): Yamaguchi, N., Suzuki, M., Fukaki, H., Morita, T. M., Tasaka, M., and Komeda, Y.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1275-1290
  • NAID: 10027004291
  • Peer Reviewed
• [Journal Article] enaoaermal-amYloplast Jess 1 is a novel allele of SHORT-ROOT. (2007)
  • Author(s): Morita, M.T.
  • Journal Title: Advances in Space Research 39 Pages: 1127-1133
  • Peer Reviewed
• [Journal Article] CRM1/BIG-mediated auxin action regulates Arabidopsis inflorescence development. (2007)
  • Author(s): Yamaguchi, N.
  • Journal Title: Plant Cell Physiol 48 Pages: 1275-1290
  • Peer Reviewed
• [Journal Article] endodermal-amyloplast less 1 is a novel allele of SHORT-ROOT. (2007)
  • Author(s): Morita, M. T.
  • Journal Title: Advances in Space Research (in press)
• [Journal Article] A C2H2-type zinc finger protein, SGR5, is involved in early events of gravitropism in Arabidopsis inflorescence stems (2006)
  • Author(s): Morita, M. T., Sakaguchi, K., Kiyose, S., Taira, K., Kato, T., Nakamura, M. and Tasaka, M.
  • Journal Title: Plant J. 47 Pages: 619-628
  • Peer Reviewed
• [Journal Article] 植物の重力屈性の分子機構 (2006)
  • Author(s): 新濱充, 森田(寺尾)美代, 田坂昌生
  • Journal Title: 植物の生長調節
  • NAID: 110002970446
• [Journal Article] A C2H2-type zinc finger protein, SGR5, is involved in early events of gravitropism in Arabidopsis inflorescence stems (2006)
  • Author(s): Morita, M. T.
  • Journal Title: Plant J. 47 Pages: 619-628
• [Journal Article] 植物の重力屈性の分子機構 (2006)
  • Author(s): 森田(寺尾)美代
  • Journal Title: Advanced Biomimetics Series 1プラントミメティックス -植物に学ぶ-(甲斐昌一、森川弘道 監修) Pages: 344-350
  • NAID: 110002970446
• [Journal Article] Shoot circumnutation and winding movements require gravisensing cells (2005)
  • Author(s): Kitazawa, D., Hatakeda, Y., Kamada, M., Fujii, N., Miyazawa, Y., Hoshino, A., Iida, S., Fukaki, F., Morita, M.T., Tasaka, M., Suge, H., and Takahashi, H.
  • Journal Title: PNAS 102 Pages: 18742-18747
  • Peer Reviewed
• [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis (2005)
  • Author(s): Niihama, M., Uemura, T., Saito, C., Nakano, A., Sato, M. H., Tasaka, M. and Morita, M. T.
  • Journal Title: Current Biology 15 Pages: 555-560
  • Peer Reviewed
• [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem (2005)
  • Author(s): Saito C., Kato T., Morita M. T. and Tasaka, M.
  • Journal Title: Plant Cell 17 Pages: 548-558
  • Peer Reviewed
• [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis. (2005)
  • Author(s): Niihama, M.
  • Journal Title: Current Biology 15 Pages: 555-560
• [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living gravi-perceptive cell of the Arabidopsis inflorescence stem. (2005)
  • Author(s): Saito C.
  • Journal Title: Plant Cell 17 Pages: 548-558
• [Journal Article] Tissue-specific expression of stabilized SORTTARY ROOT/IAA14 alters lateral root development in Arabidopsis (2005)
  • Author(s): Fukaki H.
  • Journal Title: Plant J. 44 Pages: 382-395
• [Journal Article] 植物の重力屈性の分子機構 (2005)
  • Author(s): 新濱充
  • Journal Title: 植物の生長調節 40 Pages: 139-145
• [Journal Article] Regulation of lateral root formation by auxin signaling in Arabidopsis (2005)
  • Author(s): Fukaki, H.
  • Journal Title: Plant Biotechnology 22 Pages: 393-399
• [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidoposis. (2005)
  • Author(s): Niihama, M.
  • Journal Title: Current Biology (in press)
• [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living gravi-perceptive cell of the Arabidopsis inflorescence stem. (2005)
  • Author(s): Saito, C.
  • Journal Title: Plant Cell 17 Pages: 548-558
• [Journal Article] The gravitropism defective 2 mutants of Arabidopsis are deficient in a protein implicated in endocytosis in Caenorhabditis elegans (2004)
  • Author(s): Silady R. A., Kato T., Lukowitz W., Sieber P., Tasaka M., and Somerville C. R.
  • Journal Title: Plant Physiol. 136 Pages: 3095-3103
  • Peer Reviewed
• [Journal Article] Gravity sensing and signaling (2004)
  • Author(s): Morita, M. T. and Tasaka, M.
  • Journal Title: Current Opinion in Plant Biology 4/6 Pages: 712-718
  • Peer Reviewed
• [Journal Article] Gravity sensing and signaling. (2004)
  • Author(s): Morta, M.T.
  • Journal Title: Current Opinion in Plant Biology 6/7 Pages: 712-718
• [Journal Article] The gravitropism defective 2 mutants of Arabidopsis are deficient in a protein, implicated in endocytosis in Caenorhabditis elegans. (2004)
  • Author(s): Silady, R.A.
  • Journal Title: Plant Physiology 136 Pages: 3095-3103
• [Journal Article] The NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON proteins (2004)
  • Author(s): Taoka, K.
  • Journal Title: Plant Journal 40 Pages: 462-473
• [Journal Article] The GURKE gene encoding an acetyl-CoA carboxylase is required for partitioning the embryo apex into three subregions in Arabidopsis. (2004)
  • Author(s): Kajiwara, T.
  • Journal Title: Plant Cell Physiol 45 Pages: 1122-1128
• [Journal Article] PIN-FORMED1 and PINOID regulate boundary formation and cotyled on development in Arabidopsis embryogenesis. (2004)
  • Author(s): Furutani, M.
  • Journal Title: Development 131 Pages: 5021-5030
• [Presentation] Qb-SNARE ZIG/VTI11欠損変異を抑圧すうzig suppressor 4の遺伝学的解析 (2009)
  • Author(s): 竹本仁子
  • Organizer: 日本植物生理学会年会第50回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] SGR9は重力感受細胞においてアクチンフィラメントとアミロプラストの相互作用を調節する (2009)
  • Author(s): 中村守貴
  • Organizer: 日本植物生理学会年会第50回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Presentation] A novel RING finger protein, SGR9, is involved in the regulation of act in-amyloplasts interaction in Arabidopsis shoot gravitropism (2008)
  • Author(s): Nakamura, M.
  • Organizer: ZOMES V
  • Place of Presentation: RIKEN Yokohama Institute
  • Year and Date: 2008-11-12
• [Presentation] A possible role of a novel RING finger protein in actin remodeling with in statocytes in shoot gravitropism (2008)
  • Author(s): Nakamura, M.
  • Organizer: 19th International Conference on Arabidopsis research
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2008-07-25
• [Presentation] Actin dynamics involved in gravity perception in Arabidopsis inflorescense stem (2008)
  • Author(s): Tasaka, M.
  • Organizer: 37th COSPAR scientific Assembly
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2008-07-15
• [Presentation] Qb-SNARE VTI11欠損変異zigの抑圧変異体解析から見えてきたシロイヌナズナ小胞輸送ネットワーク (2008)
  • Author(s): 森田(寺尾)美代
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-22
• [Presentation] シロイヌナズナ重力屈性における重力感受機構の解析 (2008)
  • Author(s): 森田(寺尾)美代
  • Organizer: 東京工業大学「次世代の植物研究に向けて」シンポシウム
  • Place of Presentation: 東京工業大学
  • Year and Date: 2008-02-02
• [Presentation] Actin dynamics involved in gravity perception in Arabidopsis inflorescence stems (2008)
  • Author(s): Tasaka, M., Nakamura, M., Morita, M. T.
  • Organizer: 37th COSPAR Scientific Assembly
  • Place of Presentation: Montreal
• [Presentation] A possible role of a novel RING finger protein in actin remodeling within statocytes (2008)
  • Author(s): Nakamura, M., Tasaka, M., Morita, M. T.
  • Organizer: 19th Internatinal Conference on Arabidopsis Research
  • Place of Presentation: Montreal
• [Presentation] A novel RING finger protein, SGR9, is involved in the regulation of actin-amyloplasts interaction in Arabidopsis shoot gravitropism (2008)
  • Author(s): Nakamura, M., Tasaka, M., Morita, M. T.
  • Organizer: ZOMES V
  • Place of Presentation: Yokohama
• [Presentation] 重力屈性におけるアミロプラスト動態と重力感受 (2008)
  • Author(s): 森田(寺尾)美代
  • Organizer: 日本植物学会第72回大会シンポジウム
  • Place of Presentation: 高知
• [Presentation] Qb-SNARE VTI11欠損変異zigの抑圧変異体解析から見えてきたシロイヌナズナ小胞輸送ネットワーク (2008)
  • Author(s): 森田(寺尾)美代, 橋口泰子, 新濱充, 田坂昌生
  • Organizer: 第49回日本植物生理学会年会シンポジウム
  • Place of Presentation: 札幌
• [Presentation] 高等植物における重力屈性の分子遺伝学的研究 (2007)
  • Author(s): 森田(寺尾)美代
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-23
• [Presentation] 重力を感じる仕組みを探る‐植物の重力感受細胞を視る (2007)
  • Author(s): 森田(寺尾)美代
  • Organizer: 第16回バイオイメージング学会学術集会
  • Place of Presentation: 東京理科大学
  • Year and Date: 2007-10-31
• [Presentation] 重力を感じる仕組みを探る-植物の重力感受細 (2007)
  • Author(s): 森田(寺尾)美代
  • Organizer: 第16回日本バイオイメージング学会学術集会
  • Place of Presentation: 東京理科大学
  • Year and Date: 2007-10-30
• [Presentation] Molecular genetic study of shoot gravitropism in Arabidopsis (2007)
  • Author(s): Miyo Terao Morita, Moritaka Nakamura, Masao Tasaka
  • Organizer: 第45回日本生物物理学会年会シンポジウム
  • Place of Presentation: 横浜
• [Presentation] endodermal-amyloplast less 1 is a novel allele of SHORT-ROOT (2007)
  • Author(s): Miyo T. Morita, Moritaka Nakamura, Chieko Saito, Akihiko Nakano and Masao Tasaka
  • Organizer: The 18th International Conference on Arabidopsis Research
  • Place of Presentation: Beijin
• [Presentation] 高等植物の重力感受に関わる小胞輸送の分子遺伝学的研究 (2006)
  • Author(s): 森田(寺尾)美代, 新濱充, 橋口泰子, 田坂昌生
  • Organizer: 日本分子生物学会シンポジウム
  • Place of Presentation: 横浜
• [Presentation] Molecular Genetic Study Of Vesicular Trafficking Involved In Gravitropism In Arabidopsis (2006)
  • Author(s): Miyo Terao Morita, Mitsuru Niihama, and Masao Tasaka
  • Organizer: The 53rd NIBB Conference Dynamic Organelles in Plants
  • Place of Presentation: Okazaki
• [Presentation] 植物の重力感受に必要な液胞機能に関与する分子ネットワーク : 液胞膜の動的性質の重要性 (2005)
  • Author(s): 森田美代, 倉増紀予子, 加藤壮英, 田坂昌生
  • Organizer: 第46回日本植物生理学会年会シンポジウム
  • Place of Presentation: 新潟
• [Presentation] Genetic analysis of the inflorescence stem gravitropism in Arabidopsis (2005)
  • Author(s): Miyo Terao Morita, Keitaro Sakaguchi, Sinichiro Kiyose, Masao Tasaka
  • Organizer: Gordon Research Conferences
  • Place of Presentation: New England
• [Presentation] Molecular genetic characterization of SGR5 encoding a zinc-finger protein required for gravitropism of Arabidopsis (2004)
  • Author(s): Miyo T. Morita, Shinichiro Kiyose, Takehide Kato, and Masao Tasaka
  • Organizer: The 15th International Conference on Arabidopsis Research
  • Place of Presentation: Berlin
• [Book] Signal transduction in gravitropism. In Plant Tropism (2007)
  • Author(s): Harrison, B. R., Morita, M. T., Masson, P. H., and Tasaka, M.
  • Publisher: Blackwell Publishing
• [Book] Plant Tropism (2007)
  • Author(s): Harrison, B.R.
  • Publisher: Blackwell Publishing
• [Book] 「重力屈性における重力感受」 Advanced Biomimetics Series 1 プラントミメティックス -植物に学ぶ-(甲斐昌一, 森川弘道 監修) (2006)
  • Author(s): 森田(寺尾)美代
  • Publisher: NTS
• [Remarks] 重力屈性.html
  • URL: http://bsw3.naist.jp/keihatsu/PROJECTS/