Studies on chloroplast biogenesis and the role of plastids in male gametogenesis

• Project/Area Number: 16085207
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Okayama University
• Principal Investigator: SAKAMOTO Wataru Okayama University, 資源生物科学研究所, 教授 (20222002)
• Co-Investigator(Kenkyū-buntansha): MATUSHIMA Ryo 岡山大学, 資源生物科学研究所, 助教 (80403476) ; TOMITA Yuko 岡山大学, 資源生物科学研究所, 助手 (90314666)
• Research Collaborator: KATO Yusuke 岡山大学, 資源生物科学研究所, 技術職員 (10437569) ; MIURA Eiko 岡山大学, 資源生物科学研究所, 博士後期 ; SOD Mergen 中国北京大学, 生命科学学院, 教授
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥74,900,000 (Direct Cost: ¥74,900,000); Fiscal Year 2008: ¥12,500,000 (Direct Cost: ¥12,500,000); Fiscal Year 2007: ¥12,500,000 (Direct Cost: ¥12,500,000); Fiscal Year 2006: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2005: ¥16,700,000 (Direct Cost: ¥16,700,000); Fiscal Year 2004: ¥16,600,000 (Direct Cost: ¥16,600,000)
• Keywords: 色素体機能・光合成 / オルガネラ / 植物分子機能 / オルガネラ工学 / 葉緑体 / プラスチド分化 / 配偶子形成 / シロイヌナズナ / 植物オルガネラ

Research Abstract

植物に固有の細胞内小器官(オルガネラ)であるプラスチドは、原核生物型の分裂様式により細胞内で増殖しながらダイナミックに形態や機能を変化させ、植物の器官形成・環境変動などに対応する。本研究では、(1)花粉(雄性配偶体)におけるプラスチドの役割と、(2)葉緑体におけるチラコイド分化、に関わる突然変異体の解析をモデル植物により進め、プラスチド機能に関わる未知の分子機構を明らかにする研究を行った。

Research Products

• [Journal Article] Chloroplast biogenesis : Plastid division, inheritance, regulation, protein import and proteome (2008)
  • Author(s): Sakamoto, W., Miyagishima, S., and Jarvis. P.
  • Journal Title: The Arabidopsis Book (Online only article) http://www.aspb.org/publications/arabidopsis/
  • Peer Reviewed
• [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescentlive imaging (2008)
  • Author(s): Matsushima. R., Hamamura, Y., Higashiyama, T., Arimura, S., Sodmergen, Tsutsumi. N., and Sakamoto. W. (2008) Mitochondrial
  • Journal Title: Plant Cell Physiol. 49 Pages: 1074-1083
  • Peer Reviewed
• [Journal Article] The model plant Medicago truncatula exhibits biparental plastidinheritance (2008)
  • Author(s): Matsushima, R., Hu, Y., Toyoda, K., Sodmergen, and Sakamoto. W.
  • Journal Title: Plant Cell Physiol. 49 Pages: 81-91
  • NAID: 10027495231
  • Peer Reviewed
• [Journal Article] Importance of the balance between protein synthesis and degradation in chloroplasts revealed by the studies of Arabidopsis yellow variegated mutants. (2008)
  • Author(s): Miura, E., Kato, Y., and Sakamoto W.
  • Journal Title: Proceedings of 14th International Congress of Photosynthesis Research (印刷中)
• [Journal Article] Multiple intracellular locations of Lon protease in Arabidopsis: Evidence for the localization of AtLon4 to chloroplasts. Plant cell Physiol., 48: 881-885 (2007)
  • Author(s): Ostersetzer, O., Kato, Y., Adam, Z., and Sakamoto, W.
  • Journal Title: Plant Cell Physionogy 48 Pages: 881-885
  • Peer Reviewed
• [Journal Article] White leaf sectors in yellow variegated 2 are formed by viable cells with undifferentiated plastids. (2007)
  • Author(s): Kato, Y., Miura, E., Matsushima, R., and Sakamoto, W.
  • Journal Title: Plant Physiology 144 Pages: 952-960
  • Peer Reviewed
• [Journal Article] The balance between protein synthesis and degradation in chloroplasts determines leaf variegation in Arabidopsis yellow variegated mutants. (2007)
  • Author(s): Miura, E., Kato, Y., Matsushima, R., Albrecht, V., Laalami' S., and Sakamoto, W.
  • Journal Title: Plant Cell 19 Pages: 1313-1328
  • Peer Reviewed
• [Journal Article] Isolation and characterization of Tyl/copia-like retrotransposons in mung bean (Vigna radiata (L.) Wilczek). (2007)
  • Author(s): Xiao, W.M., Su, Y., Sakamoto, W., Sodmergen
  • Journal Title: Journal of Plant Reserch 120(in press)
• [Journal Article] Protein degradation machineries in plastids. (2006)
  • Author(s): Sakamoto, W.
  • Journal Title: Annual Review Plant Biology 57 Pages: 599-621
• [Journal Article] Activation of an ER-body-localized β-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana. (2005)
  • Author(s): Nagano, A.J., Matsushima, R., Hara-Nishimura, I.
  • Journal Title: Pant and Cell Physology 46 Pages: 1140-1148
  • NAID: 10016622407
• [Journal Article] FtsH proteases in chloroplasts and cyanobacteria. (2005)
  • Author(s): Adam, Z., Zaltsman, A., Sinvany-Villalobo, G., Sakamoto, W.
  • Journal Title: Physiologia Plantarum 123 Pages: 386-390
• [Journal Article] Allelic characterization of the leaf-variegated mutation var2 identifies the conserved amino acid residues of FtsH important for ATP hydrolysis and proteolysis. (2005)
  • Author(s): Sakamoto, W., Miura, E., Kaji, Y., Okuno, T., Nishizono, M., Ogura, T.(2004)
  • Journal Title: Plant Molecular Biology (印刷中)
• [Journal Article] FtsH proteases in chloroplasts and cyanobacteria. (2005)
  • Author(s): Adam, Z., Zaltsman, A., Sinvany-Villalobo, G., Sakamoto, W.
  • Journal Title: Physiol Planta. (印刷中)
• [Journal Article] (2008) Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging
  • Author(s): Matsushima, R., Hamamura, Y., Higashiyama, T., Arimura, S., Sodmergen, Tsutsumi, N., and Sakamoto,W
  • Journal Title: Plant Cell Physiol., 49 Pages: 1074-1083
  • Peer Reviewed
• [Journal Article] (2008) The model plant Medicago truncatula exhibits biparental plastid inheritance
  • Author(s): Matsushima, R., Hu, Y., Toyoda, K., Sodmergen, and Sakamoto, W
  • Journal Title: Plant Cell Physiol., 49 Pages: 81-91
  • Peer Reviewed
• [Journal Article] (2007) Multiple intracellular locations of Lon protease in Arabidopsis : Evidence for the localization of AtLon4 to chloroplasts
  • Author(s): Ostersetzer, O., Kato, Y., Adam, Z.,and Sakamoto, W
  • Journal Title: Plant Cell Physiol., 48 Pages: 881-885
  • Peer Reviewed
• [Journal Article] (2007) White leaf sectors in yellow variegated 2 are formed by viable cells with undifferentiated plastids
  • Author(s): Kato, Y., Miura, E., Matsushima, R., and Sakamoto, W
  • Journal Title: Plant Physiol., 114 Pages: 952-960
  • Peer Reviewed
• [Journal Article] (2007) The balance between protein synthesis and degradation in chloroplasts determines leaf variegation in Arabidopsis yellow variegated mutants
  • Author(s): Miura, E., Kato, Y., Matsushima, R., Albrecht, V., Laalami, S., and Sakamoto, W
  • Journal Title: Plant Cell 19 Pages: 1313-1328
  • Peer Reviewed
• [Journal Article] (2006) Differences in DNA amount in each mitochondrion in tobacco cultured cell and rice root
  • Author(s): Takanashi, H., Arimura, S., Sakamoto, W., and Tsutsumi, N
  • Journal Title: Syst 81 Pages: 599-621
  • Peer Reviewed
• [Journal Article] (2006) Protein degradation machineries in plastids
  • Author(s): Sakamoto, W
  • Journal Title: Annu. Rev. Plant Biol., 57 Pages: 599-621
• [Journal Article] Isolation of mutants with aberrant mitochondrial morphology from Arabidopsis thaliana
  • Author(s): Feng, X., Arimura, S., Hirano, H.Y., Sakamoto, W., and Tsutsumi, N
  • Journal Title: Genes Genet. Syst., 79 Pages: 301-305
  • NAID: 10014217551
  • Peer Reviewed
• [Journal Article] (2004) Allelic characterization of the leaf-variegated mutation var2 identifies the conserved amino acid residues of FtsH important for ATP hydrolysis and proteolysis. Plant Mol
  • Author(s): Sakamoto, W., Miura, E., Kaji, Y., Okuno, T., Nishizono, M., Ogura, T
  • Journal Title: Biol., 56 Pages: 705-716
  • Peer Reviewed
• [Presentation] 花粉においてオルガネラDNAの分解を担う遺伝子の単離と解析 (2009)
  • Author(s): 松島良・Lay Yin Tang・蘇都莫日根・David Twell・坂本亘
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] D1タンパク質分解の調節機構におけるSTN8リン酸化酵素の影響 (2009)
  • Author(s): 加藤裕介・三浦栄子・坂本亘
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] The FtsH heterocomplex in chloroplasts : Dispensability of the zinc binding domain in Arabidopsis FtsH2 (2009)
  • Author(s): Di Zhang・Yusuke Kato・Sodmergen・Wataru Sakamoto
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 花粉における細胞質ゲノムの分解機構に関する研究 (2008)
  • Author(s): 松島良・坂本亘
  • Organizer: 国立遺伝学研究所研究集会「高等植物の生殖過程を制御する因子と生殖機構」
  • Place of Presentation: 三島
  • Year and Date: 2008-11-05
• [Presentation] Functional analysis of chloroplast FtsHs involved in protein quality control (2008)
  • Author(s): Sakamoto W
  • Organizer: Gordon Research Conference on Mitochondria and Chloroplasts
  • Place of Presentation: Biddeford, Maine, USA
  • Year and Date: 2008-08-13
• [Presentation] 葉緑体FtsH プロテアーゼによるD1分解機構 (2008)
  • Author(s): 加藤裕介・三浦栄子・坂本亘
  • Organizer: 日本植物生理学会第49回年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] 葉緑体FtsHプロテアーゼによるD1分解機構 (2008)
  • Author(s): 加藤祐介、三浦栄子、坂本亘
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-19
• [Presentation] Pollen organellar DNA retention in ribonucleotide reductase mutants of Arabidopsis (2008)
  • Author(s): Lay Yin Tang, Ryo Matsushima, Wataru Sakamoto
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-19
• [Presentation] Rescue of leaf variegation in var2 by over-expressing a mutated version of FtsH2 (2008)
  • Author(s): Di Zhang, Yusuke Kato, Ryo Matsushima, sodmergen, Wataru Sakamoto
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-19
• [Presentation] Genetic dissection of leaf variegation in Arabidopsis (2007)
  • Author(s): Wataru Sakamoto
  • Organizer: International Symposium on Gene Expression Control and Genome Evolution
  • Place of Presentation: 岡山
  • Year and Date: 2007-09-13
• [Presentation] The balance between chloroplast protein synthesis and degradation as an important factor of chloroplast biogenesis and maintenance (2007)
  • Author(s): Wataru Sakamoto、Eiko Miura, Yusuke Kato
  • Organizer: 14th International Congress of Phtosynthesis
  • Place of Presentation: Glasgow
  • Year and Date: 2007-07-23
• [Presentation] The balance between protein synthesis and degradation as an important factor of chloroplast biogenesis : an overview of genetic studies in organelle FtsH metalloproteases (2007)
  • Author(s): Sakamoto W
  • Organizer: International Congress on Plant Mitochondrial Biology, ICPMB2007
  • Place of Presentation: Nara, Japan
  • Year and Date: 2007-06-26
• [Presentation] The balance between protein synthesis and degradation as an important factor of chloroplast biogenesis: an overview of genetic studies in organelle FtsH metalloproteaeses (2007)
  • Author(s): Wataru Sakamoto
  • Organizer: International Congress on Plant Mitochondrial Biology, ICPMB2007
  • Place of Presentation: 奈良
  • Year and Date: 2007-06-26
• [Presentation] シロイヌナズナ斑入り変異抑制におけるメカニズムの解明 (2007)
  • Author(s): 三浦栄子・加藤裕介・松島良・坂本亘
  • Organizer: 日本植物生理学会第48回年会
  • Place of Presentation: 松山
  • Year and Date: 2007-03-27
• [Presentation] 斑入り変異体var2に生じる白色セクターは生細胞により構築される (2007)
  • Author(s): 加藤裕介・三浦栄子・松島良・坂本亘
  • Organizer: 日本植物生理学会第48回年会
  • Place of Presentation: 松山
  • Year and Date: 2007-03-27
• [Presentation] Genetic dissection of leaf variegation : a study in the Arabidopsis var2 mutant (2006)
  • Author(s): Sakamoto W
  • Organizer: The 53rd National Institute of Basic Biology Conference
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2006-07-17
• [Presentation] モデル植物系を用いたオルガネラDNAの母性遺伝に関する研究 (2006)
  • Author(s): 松島良・服部千恵子・蘇都莫日根・坂本亘
  • Organizer: 日本植物生理学会第47回年会
  • Place of Presentation: つくば
  • Year and Date: 2006-03-19
• [Presentation] 斑入り突然変異体var2では高レベルの活性酸素が蓄積する (2006)
  • Author(s): 三浦栄子・松島良・坂本亘
  • Organizer: 日本植物生理学会第47回年会
  • Place of Presentation: つくば
  • Year and Date: 2006-03-19
• [Presentation] 花粉におけるオルガネラDNA量の制御に異常を示す変異体の解析 (2006)
  • Author(s): 久保美和・松島良・服部千恵子・坂本亘
  • Organizer: 日本植物生理学会第47回年会
  • Place of Presentation: つくば
  • Year and Date: 2006-03-19
• [Presentation] Chloroplast metalloproteases in Arabidopsis-an overview of genetic and biochemical studies (2005)
  • Author(s): Sakamoto W
  • Organizer: 15th Symposium of Malaysian Society of Molecular Biology and Biotechnology
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2005-04-05
• [Presentation] FtsH metalloproteases VAR1 and VAR2 involved in the repair cycle of Photosystem (2004)
  • Author(s): Sakamoto W
  • Organizer: 13th International Congress of Photosynthesis
  • Place of Presentation: Montreal, Canada,
  • Year and Date: 2004-08-30
• [Book] The Arabidopsis Book (American Society of Plant Biologists) (2008)
  • Author(s): Sakamoto, W., Miyagishima, S., and Jarvis, P
  • Publisher: Chloroplast biogenesis : Plastid division, inheritance, regulation, protein import and proteome
  • URL: http://www.bioone.org/doi/full/10.1199/tab.0110
• [Book] 蛋白質核酸酵素増刊号,二層膜オルガネラの遺伝学(林純一・杉山康雄・坂本亘・田中寛・正木春彦編,共立出版) (2005)
  • Author(s): 坂本亘
  • Publisher: 葉緑体分化とタンパク質分解系-ATP依存性プロテアーゼと葉緑体ダイナミクス
• [Book] 生化学(日本生化学会) (2005)
  • Author(s): 坂本亘
  • Publisher: 葉緑体の膜プロテアーゼによるタンパク質の品質管理