Organelle Differentiation as the Strategy for Environmental Adaptation in Plants

• Project/Area Number: 16085209
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: NISHIMURA Mikio National Institute for Basic Biology, 高次細胞機構研究部門, 教授 (80093061)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Makoto 基礎生物学研究所, 高次細胞機構研究部門, 准教授 (50212155)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥163,600,000 (Direct Cost: ¥163,600,000); Fiscal Year 2008: ¥31,100,000 (Direct Cost: ¥31,100,000); Fiscal Year 2007: ¥31,100,000 (Direct Cost: ¥31,100,000); Fiscal Year 2006: ¥33,800,000 (Direct Cost: ¥33,800,000); Fiscal Year 2005: ¥33,800,000 (Direct Cost: ¥33,800,000); Fiscal Year 2004: ¥33,800,000 (Direct Cost: ¥33,800,000)
• Keywords: 環境 / 細胞・組織 / 植物 / バイオテクノロジー / オルガネラ / ベルオキシソーム形成因子 / タンパク質の輸送 / PEX10 / 小胞体 / クチクラワックス / ダイナミン / ポリアミン / 新規ペルオキシソーム膜タンパク質 / ペルオキシソーム / PEX遺伝子 / apm変異株 / プロテオーム / HSP90 / 熱ショック転写制御因子 / RNAi法 / タンパク質の細胞内輸送 / ダイナミン様タンパク質 / β酸化欠損変異体

Research Abstract

光照射や老化など環境適応反応においてペルオキシソームの柔軟な機能分化が重要な役割を担っている。本研究では、ペルオキシソームに焦点を絞ったトランスクリプトーム解析およびプロテオーム解析や、ペルオキシソーム異常突然変異体を用いた解析、および光照射や老化などに対するペルオキシソームの応答を網羅的に解析することによって、植物細胞の環境戦略を支えるペルオキシソーム機能分化に関わる因子を同定し、その機能解析からペルオキシソーム機能分化の全体像を明らかにした。

Research Products

• [Journal Article] The plant organelles database (PODB): a collection of visualized plant organelles and protocols for plant organelle research. (2008)
  • Author(s): Mano, S., T. Niwa, S. Nishikawa, T. Mimura, M. Nishimura
  • Journal Title: Nucleic Acid Res. 36
  • Peer Reviewed
• [Journal Article] NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. (2008)
  • Author(s): Yamada, K., A.J. Nagano, M. Nishina, I. Hara-Nishimura, M. Nishimura
  • Journal Title: Plant Cell 20 Pages: 2529-2540
  • Peer Reviewed
• [Journal Article] NAC family proteins NARS1and NARS2 in the outer integument regulate embryogenesis in Arabidopsis. (2008)
  • Author(s): Kunieda T., N. Mitsuda, M. Ohme-Takagi, S. Takeda, M. Aida, M. Tasaka, M. Kondo, M. Nishimura, I. Hara-Nishimura
  • Journal Title: Plant Cell 20 Pages: 2631-2642
  • Peer Reviewed
• [Journal Article] Proteomic identification and characterization of a novel peroxisomal adenine nucleotide transporter supplying ATP for fatty acid β-oxidation in soybean and Arabidopsis. (2008)
  • Author(s): Arai, Y., M. Hayashi, M. Nishimura
  • Journal Title: Plant Cell 20 Pages: 3227-3240
  • Peer Reviewed
• [Journal Article] Proteomic identification and characterization of a novel peroxisomal adenine nucleotide transporter supplying ATP for fatty acid β-oxidantion in soybean and Arabidopsis. (2008)
  • Author(s): Arai Yuko
  • Journal Title: Plant Cell 20 Pages: 3227-3240
  • Peer Reviewed
• [Journal Article] Proteomic analysis of highly purified peroxisomes from etiolated soybean cotyledons. (2008)
  • Author(s): Arai Yuko
  • Journal Title: Plant Cell Physiol. 49 Pages: 526-539
  • Peer Reviewed
• [Journal Article] An isoform of Arabidopsis myosin XI interacts with small GTPases in its'c-terminal tail region. (2008)
  • Author(s): Hashimoto Kohsuke
  • Journal Title: J.Exp.Bot. 59 Pages: 3523-3531
  • Peer Reviewed
• [Journal Article] A Putative peroxisomal polyamine oxidase, AtPAO4. is involved in polyamine catabolism in Arabidopsis thaliana. (2008)
  • Author(s): Kamada-Nobusada Tomoe
  • Journal Title: Plant Cell Physiol. 49 Pages: 1272-1282
  • Peer Reviewed
• [Journal Article] NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis. (2008)
  • Author(s): Kunieda Tadashi
  • Journal Title: Plant Cell 20 Pages: 2631-2642
  • Peer Reviewed
• [Journal Article] The plant organelles database (PODB): A collection of visualized plant organelles and protocols for plant organelle research. (2008)
  • Author(s): Mano Shoji
  • Journal Title: Nucleic Acids Res. 36
  • Peer Reviewed
• [Journal Article] Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis. (2008)
  • Author(s): Mitsuhashi Naoto
  • Journal Title: J.Exp.Bot. 59 Pages: 3069-3076
  • Peer Reviewed
• [Journal Article] Antagonistic jacalin-related lectins regulate the size of er body-type β-glucosidase complexes in Arabidopsis thaliana. (2008)
  • Author(s): Nagano Atsushi
  • Journal Title: Plant Cell Physiol. 49 Pages: 969-980
  • Peer Reviewed
• [Journal Article] Atmapl : a DNA microarray for genomic deletion mapping in Arabidopsis thaliana. (2008)
  • Author(s): Nagano Atsushi
  • Journal Title: Plant J. 56 Pages: 1058-1065
  • Peer Reviewed
• [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import. (2008)
  • Author(s): Oshima Yoshimi
  • Journal Title: Plant J. 49 Pages: 671-677
  • Peer Reviewed
• [Journal Article] Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice. (2008)
  • Author(s): Tani Tomoyuki
  • Journal Title: Planta 227 Pages: 517-526
  • Peer Reviewed
• [Journal Article] NA12 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. (2008)
  • Author(s): Yamada Kenji
  • Journal Title: Plant Cell 20 Pages: 2529-2540
  • Peer Reviewed
• [Journal Article] Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana. (2008)
  • Author(s): Yamada Kenji
  • Journal Title: Plant Signal.Behav. 3 Pages: 660-662
  • Peer Reviewed
• [Journal Article] Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence. (2008)
  • Author(s): Yamazaki Misako
  • Journal Title: Plant Cell Physiol. 49 Pages: 142-156
  • Peer Reviewed
• [Journal Article] The plant organelles database (PODB): A collection of visualized plant organelles and protocols for plant organelle research. (2008)
  • Author(s): Mano, S., T. Miwa, S. Nishikawa, T. Mimura and M. Nishimura
  • Journal Title: Nucleic Acid Res. 36(Databaseissue)
  • Peer Reviewed
• [Journal Article] Proteomic analysis of highly purified peroxisomes from etiolated soybean cotyledons. (2008)
  • Author(s): Arai, Y., M. Hayashi and M. Nishimura
  • Journal Title: Plant Cell Physiology (In press)
  • NAID: 10027330156
  • Peer Reviewed
• [Journal Article] Suzuki and M. Nishimura Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana. (2007)
  • Author(s): Yamada, K., M. Fukazawa, M. Hayashi, I. Suzuki, M. Nishimura
  • Journal Title: J. Biol. Chem. 282 Pages: 37794-37804
  • Peer Reviewed
• [Journal Article] Functional classification of Arabidopsis peroxisome biogenesis factors proposed form analyses of knockdown mutants. (2007)
  • Author(s): Nito, K., A. Kamigaki, M. Kondo, M. Yagi, M. Hayash, M. Nishimura
  • Journal Title: Plant Cell Physiol. 48 Pages: 763-774
  • Peer Reviewed
• [Journal Article] Functional classification of Arabidopsis peroxisome biogenesis factors proposed from analyses of knockdown mutants. (2007)
  • Author(s): Nito, K., A Kamigaki, M. Kondo, M. Hayashi and M. Nishimura
  • Journal Title: Plant Cell Physiol. 48 Pages: 763-774
  • NAID: 10027002145
  • Peer Reviewed
• [Journal Article] HSP90 regulates the heat shock response in Arabidopsis thaliana that is responsible for heat adaptation. (2007)
  • Author(s): Yamada, K., M. Fukazawa, M. Hayashi, I. Suzuki and M. Nishimura
  • Journal Title: J. Biol. Chem. 282 Pages: 37794-37804
  • Peer Reviewed
• [Journal Article] The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2- dependent protein transport to peroxisomes. (2006)
  • Author(s): Mano, S., C. Nakamori, K. Nito, M. Kondo, M. Nishimura
  • Journal Title: Plant J. 47 Pages: 604-618
  • Peer Reviewed
• [Journal Article] Arabidopsis thaliana-A model organism to study plant peroxisomes Biophys. (2006)
  • Author(s): Hayashi, M, M. Nishimura
  • Journal Title: Biochim. Acta 1763 Pages: 1382-1391
  • Peer Reviewed
• [Journal Article] AtVAM3 is required for normal specification of idioblasts, myrosin cells. (2006)
  • Author(s): Ueda, H.
  • Journal Title: Plant Cell Physiol. 47 Pages: 164-175
• [Journal Article] The Arabidopsis pex12 and pex13 mutants are defective in both PTS1 and PTS-2dependent transport to peroxisomes. (2006)
  • Author(s): Mano. S.
  • Journal Title: Plant J. 47 Pages: 1187-1194
• [Journal Article] AtVPS29, a Putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. (2006)
  • Author(s): Shimada, T.
  • Journal Title: Plant Cell Physiol. 47 Pages: 1187-1194
• [Journal Article] The cellular suicide strategy of plants : Vacuole-mediated cell death (2006)
  • Author(s): Hatsugai, N.
  • Journal Title: Apoptosis 11 Pages: 905-911
• [Journal Article] Arabidopsis thaliana-A Model Organism to Study Plant Peroxisomes. (2006)
  • Author(s): Hayashi, M., Nishimura, M.
  • Journal Title: Biochim. Biophys. Acta 1763 Pages: 1382-1391
• [Journal Article] Differential contribution of two peroxisomal protein receptors to the maintenance of peroxisomal functions in Arabidopsis. (2005)
  • Author(s): Hayashi, M., K. Nito, M. Yagi, T. Kamada, M. Nishimura
  • Journal Title: J. Biol. Chem. 280 Pages: 14829-14835
  • Peer Reviewed
• [Journal Article] (2005)
  • Author(s): Mano, S., M. Nishimura
  • Journal Title: Plant peroxisomes Vitamin and Hormones(Elsevier Sci. Press) 72 Pages: 111-154
  • Peer Reviewed
• [Journal Article] Vacuolar processing enzyme : an executor of plant cell death. (2005)
  • Author(s): Hara-Nishimura, I.
  • Journal Title: Curr. Opin. Plant Biol. 8 Pages: 404-408
• [Journal Article] Differential contribution of two peroxisomal protein receptors to the maintenance of peroxisomal functions in Arabidopsis. (2005)
  • Author(s): Hayashi, M.
  • Journal Title: J.Biol.Chem. 280 Pages: 14829-14835
• [Journal Article] The involvement of tonoplast proton pumps and Na+(K+)/H+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv.Heavenly Blue. (2005)
  • Author(s): Yoshida, K.
  • Journal Title: Plant Cell Physiol. 46 Pages: 407-415
• [Journal Article] Peroxisomal localization of a myosin XI isoform in Arabidopsis thaliana. (2005)
  • Author(s): Hashimoto, K.
  • Journal Title: Plant Cell Physiol. 46 Pages: 782-789
• [Journal Article] Vacuolar processing enzyme is essential for microtoxin-induced cell death in Arabidopsis thaliana. (2005)
  • Author(s): Kuroyanagi, M.
  • Journal Title: J.Biol.Chem. 280 Pages: 32914-32920
• [Journal Article] A defect in glyoxysomal fatty acid b-oxidation reduces jasmonic acid accumulation in Arabidopsis. (2005)
  • Author(s): Afitlhile, M.M.
  • Journal Title: Plant Physiol.Biochem. 43 Pages: 603-609
• [Journal Article] An Arabidopsis dynamin-related protein, DRP3A, controls both peroxisomal and mitochondrial division. (2004)
  • Author(s): Mano, S., C. Nakamori, M. Kondo, M. Hayashi, M. Nishimura
  • Journal Title: Plant J. 38 Pages: 487-498
  • Peer Reviewed
• [Journal Article] Diversity and formation of endoplasmic reticulum-derived compartments in plants. Are these compartments specific to plant cells? (2004)
  • Author(s): Hara-Nishimura, I., R. Matsushima, T. Shimada, M. Nishimura
  • Journal Title: Plant Physiol. 136 Pages: 3435-3439
  • Peer Reviewed
• [Journal Article] An Arabidopsis dynamin-related protein, DRP3A, controls both peroxisomal and mitochondrial division. (2004)
  • Author(s): Shoji Mano
  • Journal Title: Plant J. 38 Pages: 487-498
• [Journal Article] Cryptochromes and phytochromes synergetically regulate the Arabidopsis root greening. (2004)
  • Author(s): Takeshi Usami
  • Journal Title: Plant Cell Physiol. 45 Pages: 1798-1808
• [Journal Article] Differential contribution of two peroxisomal protein receptors to the maintenance of peroxisomal functions in Arabidopsis.
  • Author(s): Makoto Hayashi
  • Journal Title: J.Biol.Chem. (in press)
• [Journal Article] The involvement of tonoplast proton pumps and Na+(K+)/H+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv Heavenly Blu.
  • Author(s): Kumi Yoshida
  • Journal Title: Plant Cell Physiol. (in press)
• [Journal Article] Peroxisomal localization of an isofoem of myosin XI, MYA2, in Arabidopsis thaliana.
  • Author(s): Kimi Hashimoto
  • Journal Title: Plant Cell Physiol. (in press)
• [Presentation] The Plant Organelles Database Version 2: The Databases for Plant Organelle Dynamics and Methods for Functional Analysis. (2008)
  • Author(s): Mano, S., Miwa, T., Nishikawa, S., Mimura, T., Nishimura M
  • Organizer: The 55th NIBB conference, Arabidopsis Workshop 2008 "Frontiers of Plant Science in the 21st Century"
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2008-09-14
• [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation. (2007)
  • Author(s): Yamada, K., Fukao, Y., Hayashi, M., Fukazawa, M., Suzuki, i., Nishimura, M
  • Organizer: 18th International Conference on Arabidopsis Research
  • Place of Presentation: Beijing, China
• [Presentation] Construction of plant organelles database. (2006)
  • Author(s): Mano, S., Miwa, T., Nishikawa, S., Mimura, T., Nishimura, M
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB congress
  • Place of Presentation: Kyoto, Japan
• [Presentation] HSP90 regulates stress response to adapt a high temperature in Arabidopsis. (2006)
  • Author(s): Yamada, K., Fukao, Yoichiro, Suzuki, I., Nishimura, M
  • Organizer: Plant Biology 2006 (ASPP 2006 Annual Meeting)
  • Place of Presentation: Boston, MA, USA
• [Presentation] Biogenesis and differentiation of plant peroxisomes. (2006)
  • Author(s): Nishimura, M., Hayashi, M., Mano, S., Nito, K., Kamada-Nobusada, T., Kondo, M., Arai, Y., Kamigaki A., Oikawa, K
  • Organizer: 53rd NIBB conference -Dynamic organelles in plants
  • Place of Presentation: Okazaki, Japan
• [Presentation] Establishment of peroxisomal functions by peroxisomal biogenesis factors. (2006)
  • Author(s): Hayashi, M., Kamigaki, A., Nishimura, M.
  • Organizer: VIII Spanish Congress on Plant Molecular Biology
  • Place of Presentation: Pamplona, Spain
• [Presentation] Plant peroxisomal differentiation revealed by transcriptomic and proteomic analyses. (2005)
  • Author(s): Kamada-Nobusada, T., Fukao, Y., Hayashi, M., Nishimura, M
  • Organizer: KEYSTONE SYMPOSIA, Plant Cell Signaling: in vivo and omics Approaches
  • Place of Presentation: Santa Fe, NM, USA
• [Presentation] Differential contribution of Pex5p and Pex7p to the maintenance of peroxisomal functions in Arabidopsis. (2005)
  • Author(s): Hayashi, M., Yagi, M., Nishimura, M
  • Organizer: Plant Biology 2005
  • Place of Presentation: Seatle, WA, USA
• [Presentation] Contribution of Pex5p and Pex7p in the maintenance of peroxisomal functions in plants. (2004)
  • Author(s): Hayashi, M., Yagi, M., Nito, K., Kamada, T., Nishimura, M
  • Organizer: International meeting on the topogenesis of organellar proteins
  • Place of Presentation: Bochum, Germany
• [Book] Peroxisome Plant Proteomics: Technologies, Strategies and Applications (2008)
  • Author(s): Arai, Y., Y. Fukao, M. Hayashi, M. Nishimura
• [Book] Peroxisome In Plant Proteomics : Technologies, Strategies and Appli cations (2008)
  • Author(s): Arai Yuko
  • Total Pages: 764
  • Publisher: Jhon Wiley & Sons
• [Book] Peroxisome In Plant Proteomics: Technologies, Strategies and Applications (2008)
  • Author(s): Arai, Y., Y. Fukao, M. Hayashi and M. Nishimura
  • Publisher: John Wiley & Sons(In press)
• [Book] Vitamin and Hormones (2005)
  • Author(s): Mano, S.
  • Total Pages: 43
  • Publisher: Elsevire
• [Remarks]
  • URL: http://podb.nibb.ac.jp/Organellome/
• [Remarks]
  • URL: http://www.nibb.ac.jp/organelles/
• [Patent(Industrial Property Rights)] 植物の発芽を抑制する方法、及び発芽が抑制された形質転換体 (2004)
  • Inventor(s): 林誠、西村幹夫、八木美奈
  • Industrial Property Rights Holder: 独立行政法人科学技術振興機構
  • Filing Date: 2004-04-07
• [Patent(Industrial Property Rights)] 種皮特異的誘導性プロモーター (2004)
  • Inventor(s): 西村いくこ、西村幹夫、山田健志、中畦悟
  • Industrial Property Rights Holder: 独立行政法人科学技術振興機構
  • Filing Date: 2004-11-30
• [Patent(Industrial Property Rights)] フザリウム毒抵抗性形質転換植物 (2004)
  • Inventor(s): 西村いくこ、黒柳美和、西村幹夫、山田健志
  • Industrial Property Rights Holder: 国立大学法人京都大学、大学共同利用機関法人自然科学研究機構
  • Filing Date: 2004-12-28