Elucidation of gene cascade regulating sex differentiation

• Project/Area Number: 16086214
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University (2008); National Institute for Basic Biology (2004-2007)
• Principal Investigator: MOROHASHI Ken-ichirou Kyushu University, 大学院・医学研究院, 教授 (30183114)
• Co-Investigator(Kenkyū-buntansha): KATOH-FUKUI Yuko 国立長寿医療センター研究所, 老化制御研究部・分子制御研究室, 室長 (50342639) ; OGAWA Hidesato 基礎生物学研究所, 性差生物学研究部門, 助教 (20370132)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥264,000,000 (Direct Cost: ¥264,000,000); Fiscal Year 2008: ¥52,800,000 (Direct Cost: ¥52,800,000); Fiscal Year 2007: ¥52,800,000 (Direct Cost: ¥52,800,000); Fiscal Year 2006: ¥52,800,000 (Direct Cost: ¥52,800,000); Fiscal Year 2005: ¥52,800,000 (Direct Cost: ¥52,800,000); Fiscal Year 2004: ¥52,800,000 (Direct Cost: ¥52,800,000)
• Keywords: 性分化 / 転写制御 / エンハンサー / 生殖腺 / Ad4BP / SF-1 / 組織特異的エンハンサー / 胎仔ライディッヒ細胞 / Emx2 / 生殖腺特異的エンハンサー / 精巣 / 卵巣 / M33 / 脳下垂体牲腺刺激ホルモン分泌細胞 / 視床下部腹内側核 / 遺伝子破壊マウス / 副腎 / 組織特異的発現 / インシュレーター / SUMO / 翻訳後修飾

Research Abstract

種々の遺伝子ノックアウトマウスを用い、雌雄の生殖腺(精巣と卵巣)の性分化過程における、Ad4BP/SF-1、Emx2、M33、Fkhl18、AhR、Wnt4、Dax-1、Sox9などの遺伝子の関係の一端を明らかにした。なかでも、ポリコーム複合体の因子であるM33が性決定遺伝子SRYを制御すること、ダイオキシン受容体AhRが卵巣にて女性ホルモン産生酵素であるアロマターゼ遺伝子を制御することなどが明らかになった。また、Ad4BP/SF-1遺伝子の組織特異的エンハンサーに着目し、精巣にて男性ホルモンを産生するライディッヒ細胞や副腎皮質細胞におけるエンハンサー、脳下垂体エンハンサー、視床下部腹内側核エンハンサーなどを同定した。これらのエンハンサーを用い、各種トランスジェニックマウスを作製し、副腎皮質とライディッヒ細胞の系譜を明らかにした。また、胎仔ライディッヒ細胞-GFPマウスを作製し、胎仔精巣にて男性ホルモンを産生する胎仔ライディッヒ細胞を単離することが可能とした。そして、純化した胎仔ライディッヒ細胞にてマイクロアレイ解析とChIP-シークエンス解析を行ってきた。その結果、胎仔ライディッヒ細胞が男性ホルモンを産生するとの従来からの説が否定され、胎仔生殖腺の性差と成獣生殖腺の性差が質的に異なることを明らかにしてきた。これらの知見は今後の性差に主眼を置く研究の基盤を提供するものである。

Research Products

• [Journal Article] Transcriptional suppression by transient recruitment of ARIP4 to sumoylated nuclear receptor Ad4BP/SF-1 (2009)
  • Author(s): H. Ogawa, T. Komatsu, Y. Hiraoka, K. Morohashi
  • Journal Title: Morohashi Mol Biol Cell 20 Pages: 4235-4245
• [Journal Article] Transgenic expression of Ad4BP/SF-1 in fetal adrenal progenitor cells leads to ectopic adrenal formation (2009)
  • Author(s): M. Zubair, S. Oka, K.L. Parker, K. Morohashi
  • Journal Title: Mol Endocrinol 23 Pages: 1657-1667
• [Journal Article] Generation of rat monoclonal antibodies specific for Ad4BP/SF-1 (2009)
  • Author(s): C. Yokoyama, T. Komatsu, H. Ogawa, K. Morohashi, M. Azuma, T. Tachibana
  • Journal Title: Hybridoma 28 Pages: 113-119
• [Journal Article] A critical time window of Sry action in gonadal sex determination (2009)
  • Author(s): R. Hiramatsu, S. Matoba, M. Fujisawa, M. Kanai- Azuma, N. Tsunekawa, M. Kurohmaru, K. Morohashi, D. Wilhelm, P. Koopman, Y. Kanai
  • Journal Title: Development 136 Pages: 129-138
• [Journal Article] A critical time window of Sry action in gonadal sex determination. (2009)
  • Author(s): Ryuji Hiramatsu, et al.
  • Journal Title: Development 136 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Developmental links between fetal and adult adrenal cortex revealed by lineage tracing (2008)
  • Author(s): M. Zubair, K.L. Parker, K. Morohashi
  • Journal Title: Mol. Cell. Biol 28 Pages: 7030-7040
• [Journal Article] Identification of NR5A1 (SF-1/AD4BP) gene expression modulators by large-scale gain and loss of function studies (2008)
  • Author(s): N. Sakai, H. Terami, S. Suzuki, M. Haga, K. Nomoto, N. Tsuchida, K. Morohashi, N. Saito, M. Asada, M. Hashimoto, D. Harada, H. Asahara, T. Ishikawa, F. Shimada, K. Sakurada
  • Journal Title: J. Endocrinol 198 Pages: 489-497
• [Journal Article] Pitx2 directly regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope via interaction with the intronic enhancer (2008)
  • Author(s): Y. Shima, M. Zubair, T. Komatsu, S. Oka, C. Yokoyama, T. Tachibana, T.A. Hjalt, K. Morohashi
  • Journal Title: Mol. Endocrinol 22 Pages: 1633-1646
• [Journal Article] Importance of forkhead transcription factor Fkhl18 for development of testicular vasculature (2008)
  • Author(s): Y. Sato, T. Baba, M. Zubair, K. Miyabayashi, Y. Toyama, M. Maekawa, A. Owaki, H. Mizusaki1, T. Sawamura, K. Toshimori, K. Morohashi, Y. Katoh-Fukui
  • Journal Title: Mol. Repro. Dev. 75 Pages: 1361-1371
• [Journal Article] Disruption of aryl hydrocarbon receptor (AhR) induces regression of the seminal vesicle in aged male mice (2008)
  • Author(s): T. Baba, Y. Shima, J. Mimura, M. Oshima, Y. Fujii- Kuriyama, K. Morohashi
  • Journal Title: Sex. Dev. 2 Pages: 1-11
• [Journal Article] Mechanism of asymmetric ovarian development in chick embryos (2008)
  • Author(s): Y. Ishimaru, T. Komatsu, M. Kasahara, Y. Katoh- Fukui, Y. Toyama, M. Maekawa, K. Toshimori, R.A.S. Chandraratna, K. Morohashi, H. Yoshioka
  • Journal Title: Development 135 Pages: 677-685
• [Journal Article] Up-regulation of SOX9 in human sex-determining region on the Y chromosome (SRY)-negative XX males (2008)
  • Author(s): Y. Kojima, Y. Hayashi, K. Mizuno, S. Sasaki, Y. Katoh-Fukui, P. Koopman, K. Morohashi, K. Kohri
  • Journal Title: Clin. Endocrinol 68 Pages: 791-799
• [Journal Article] Mastermind-like domain containing 1 (MAMLD1 or Cxorf6) transactivates the Hes3 promoter, augments testosterone production, and contains the SF1 target sequence (2008)
  • Author(s): M. Fukami, Y. Wada, M. Okada, F. Kato, N. Katsumata, T. Baba, K. Morohashi, J. Laporte, M. Kitagawa, T. Ogata
  • Journal Title: J. Biol. Chem. 283 Pages: 5525-5532
• [Journal Article] Mastermind-like domain containing 1 (MAMLD1 or Cxorf6) transactivates the Hes3 promoter, augments testosterone production, and contains the SF1 target sequence. (2008)
  • Author(s): Maki Fukami, et al.
  • Journal Title: J.Biol.Chem. 283 Pages: 5525-5532
  • Peer Reviewed
• [Journal Article] Mechanism of asymmetric ovarian development in chick embryos. (2008)
  • Author(s): Yoshiyasu Ishimaru ,et al.
  • Journal Title: Development 135 Pages: 677-685
  • Peer Reviewed
• [Journal Article] Up-regulation of SOX9 in human sex-determining region on the Y chromosome (SRY)-negative XX males. (2008)
  • Author(s): Yoshiyuki Kojima, et al.
  • Journal Title: Clin.Endocrinol. 68 Pages: 791-799
  • Peer Reviewed
• [Journal Article] Disruption of aryl hydrocarbon receptor (AhR) induces regression of the seminal vesicle in aged male mice. (2008)
  • Author(s): Takashi Baba, et al.
  • Journal Title: Sex.Dev. 2 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Importance of forkhead transcription factor Fkh118 for development of testicular vasculature. (2008)
  • Author(s): Yuko Sato, et al.
  • Journal Title: Mol.Repro.Dev. 75 Pages: 1361-1371
  • Peer Reviewed
• [Journal Article] Pitx2 directly regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotroupe via interaction with the intronic enhancer. (2008)
  • Author(s): Yuichi Shima, et al.
  • Journal Title: Mol.Endocrinol. 22 Pages: 1633-1646
  • Peer Reviewed
• [Journal Article] Identification of NR5A1 (SF-1/AD4BP) gene expression modulators by large-scale gain and loss of function studies. (2008)
  • Author(s): Noriko Sakai, et al.
  • Journal Title: J.Endocrinol. 198 Pages: 489-497
  • Peer Reviewed
• [Journal Article] Developmental links between fetal and adult adrenal cortex revealed by Lineage tracing (2008)
  • Author(s): Mohamad Zubair, et al.
  • Journal Title: Mol.Cell.Biol. 28 Pages: 7030-7040
  • Peer Reviewed
• [Journal Article] Generation of rat monoclonal antibodies specific for Ad4BP/SF-1. (2008)
  • Author(s): Chikako Yokoyama, et al.
  • Journal Title: Hybridoma 28 Pages: 113-119
  • Peer Reviewed
• [Journal Article] Mechanism of asymmetric ovarian development in chick embryos (2008)
  • Author(s): Y. Ishimaru, et. al.
  • Journal Title: Development 135 Pages: 677-685
  • Peer Reviewed
• [Journal Article] Germ cells are essential for sexual dimorphism in the medaka gonad (2007)
  • Author(s): H. Kurokawa, D. Saito, Y. Katoh-Fukui, K. Ohta, Takashi Baba, K. Morohashi, M. Tanaka
  • Journal Title: Pro. Natl. Acad. Sci. USA 104 Pages: 16958-16963
• [Journal Article] Liver receptor homologue-1 (LRH-1) activates the promoter of brain aromatase (Cyp19a2) in a teleost fish, the medaka, Oryzias latipes (2007)
  • Author(s): Y. Ohmuro-Matsuyama, K. Okubo, M. Matsuda, S. Ijiri, D. Wang, G. Guan, T. Suzuki, M. Matsuyama, K. Morohashi, Y. Nagahama
  • Journal Title: Mol. Repro. Dev. 74 Pages: 1065-1071
• [Journal Article] Atrazine-induced aromatase expression is SF-1- dependent: Implications for endocrine disruption in wildlife and reproductive cancers in humans (2007)
  • Author(s): W. Fan, T. Yanase, H. Morinaga, S. Gondo, T. Okabe, M. Nomura, T. Komatsu, K. Morohashi, T.B. Hayes, R. Takayanagi, H. Nawata
  • Journal Title: Environmental Health Perspectives 115 Pages: 720-727
• [Journal Article] Foxl2 up-regulates aromatase gene transcription in a female-specific manner by binding to the promoter as well as interacting with Ad4BP/SF-1 (2007)
  • Author(s): D. Wang, T. Kobayashi, L. Shou, Y. Ohmuro- Matsuyama, G. Guan, S. Ijiri, F. Sakai, M. Matsuda, Y. Shibata, K. Okubo, K. Morohashi, Y. Nagahama
  • Journal Title: Mol. Endocrinol. 21 Pages: 712-725
• [Journal Article] Hypogonadotropic hypogonadism in an adult female with a heterozygous hypomorphic mutation of SOX2 (2007)
  • Author(s): N. Sato, Y. Kamachi, H. Kondoh, Y. Shima, K. Morohashi, R. Horikawa, T. Ogata
  • Journal Title: Eur. J. Endocrinol. 156 Pages: 169-173
• [Journal Article] Fox12 up-regulates aromatase gene transcription in a female-specific manner by binding to the promoter as well as interactingwith Ad4BP/SF-1 (2007)
  • Author(s): D-S. Wang, et. al.
  • Journal Title: Mol. Endccrinol. 21 Pages: 712-725
  • Peer Reviewed
• [Journal Article] Germ cells are essential for sexual dimorphism in the medaka gonad (2007)
  • Author(s): H. Kurokawa, et. al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 16958-16963
  • Peer Reviewed
• [Journal Article] Hypogonadotropic hypogonadism in an adult female with a heterozygous hypomorphic mutation of SOX2. (2007)
  • Author(s): N.Sato, Y.Kamachi, H.Kondoh, Y.Shima, K.Morohashi, R.Horikawa, T.Ogata
  • Journal Title: Eur. J. Endocrinol. 156 Pages: 169-173
• [Journal Article] Fox12 up-regulates aromatase gene transcription in a female-specific manner by binding to the promoter as well as interacting with Ad4BP/SF-1. (2007)
  • Author(s): D-S.Wang, T.Kobayashi, L-Y.Shou, Y.Ohmuro-Matsuyama, G-J.Guan, S.Ijiri, F.Sakai, M.Matsuda, Y.Shibata, K.Okubo, K.Morohashi, Y.Nagahama
  • Journal Title: Mol. Endocrinol. (in press)
• [Journal Article] CXorf6 is a causative gene for hypospadias (2006)
  • Author(s): M. Fukami, Y. Wada, K. Miyabayashi, I. Nishino, T. Hasegawa, G. Camerino, C. Kretz, A. Buj- Bello, J. Laporte, G. Yamada, K. Morohashi, T. Ogata
  • Journal Title: Nature Genetics 38 Pages: 1369-1371
• [Journal Article] Role of transcription factors Ad4BP/SF-1 and DAX-1 in steroidogenesis and spermatogenesis in human testicular development and idiopathic azospermia (2006)
  • Author(s): Y. Kojima, S. Sakaki, Y. Hayashi, Y. Umemoto, K. Morohashi, K. Kohri
  • Journal Title: Int. J. Urol. 13 Pages: 785-793
• [Journal Article] Two-step regulation of Ad4BP/SF-1 gene transcription during fetal adrenal development; initiation by a Hox-Pbx1-Prep1 complex and maintenance via autoregulation by Ad4BP/SF-1 (2006)
  • Author(s): M. Zubair, S. Ishihara, S. Oka, K. Okumura, K. Morohashi
  • Journal Title: Mol. Cell. Biol. 26 Pages: 4111-4121
• [Journal Article] Differential gene dosage effects of Ad4BP/SF-1 on target tissue development (2006)
  • Author(s): Fatchiyah, M. Zubair, Y. Shima, S. Oka, S. Ishihara, Y. Fukui-Katoh, K. Morohashi
  • Journal Title: Biochem. Biophys. Res. Commun. 341 Pages: 1036-1045
• [Journal Article] Differential gene dosage effects of Ad4BP/SF-1 on target tissue development. Biochem. Biophys (2006)
  • Author(s): Fatchiyah, M., Zubair, Y., Shima, S., Oka, S., Ishihara, Y., Fukui-Katoh, K.Morohashi
  • Journal Title: Res. Commun. 341 Pages: 1006-1045
• [Journal Article] Two-step regulation of Ad4BP/SF-1 gene transcription during fetal adrenal development; initiation by a Hox-Pbx1-Prep1 complex and maintenance via autoregulation by Ad4BP/SF-1. (2006)
  • Author(s): M.Zubair, S.Ishihara, S.Oka, K.Okumura, K.Morohashi
  • Journal Title: Mol. Cell. Biol. 26 Pages: 411-4121
• [Journal Article] Role of transcription factors Ad4BP/SF-1 and DAX-1 in steroidogenesis and spermatogenesis in human testicular development and idiopathic azospermia. (2006)
  • Author(s): Y.Kojima, S.Sakaki, Y.Hayashi, Y.Umemoto, K.Morohashi, K.Kohri
  • Journal Title: Int. J. Urol. 13 Pages: 785-793
• [Journal Article] CXorf6 is a causative gene for hypospadias. (2006)
  • Author(s): M.Fukami, Y.Wada, K.Miyabayashi, I.Nishino, T.Hasegawa, G.Camerino, C.Kretz, A.Buj-Bello, J.Laporte, Gen Yamada, K.Morohashi, T.Ogata
  • Journal Title: Nature Genetics 38 Pages: 1369-1371
• [Journal Article] Ah (dioxin) receptor as a key factor in the regulation of female reproduction (2005)
  • Author(s): T. Baba, J. Mimura, N. Nakamura, N. Harada, M. Yamamoto, K. Morohashi, Y. Fujii-Kuriyama
  • Journal Title: Mol. Cell. Biol. 25 Pages: 10040-10051
• [Journal Article] VMH specific enhancer of Ad4BP/SF-1 gene (2005)
  • Author(s): Y. Shima, M. Zubair, S. Ishihara, Y. Shinohara, S. Oka, S. Kimura, S. Suita, K. Morohashi
  • Journal Title: Mol. Endocrinol. 19 Pages: 2812-2823
• [Journal Article] Mouse Polycomb M33 is required for splenic vascular and adrenal gland formation through regulating Ad4BP/SF-1 expression (2005)
  • Author(s): Y. Katoh-Fukui, A. Owaki, Y. Sotoyama, M. Kusaka, Y. Shinohara, M. Maekawa, K. Toshimori, K. Morohashi
  • Journal Title: Blood 106 Pages: 1612-1620
• [Journal Article] A steroidogenic cell line with differentiation potential from mouse granulosa cells, transfected with Ad4BP and SV40 large T antigen genes (2005)
  • Author(s): Kamei Y, Aoyama Y, Fujimoto T, Kenmotsu N, Kishi C, Koushi M, Sugano S, Morohashi K, Kamiyama R, Asakai R.
  • Journal Title: J. Endocrinol. 185 Pages: 187-195
• [Journal Article] Novel isoform of vinexin, vinexin g, regulates Sox9 gene expression through activation of MAPK cascade in mouse fetal gonad (2005)
  • Author(s): M. Matsuyama, H. Mizusaki, A. Shimono, T. Mukai, K. Okumura, K. Abe, K. Shimada, K. Morohashi
  • Journal Title: Genes Cells 10 Pages: 421-434
• [Journal Article] Mesonephric FGF signaling is associated with the development of sexually indifferent gonadal primordium in chick embryos (2005)
  • Author(s): H. Yoshioka, Y. Ishimaru, N. Sugiyama, N. Tsunekawa, T. Noce, M. Kasahara, K. Morohashi
  • Journal Title: Dev. Biol. 280 Pages: 150-161
• [Journal Article] Identification of the boundary for histone acetylation between nuclear receptor genes, Ad4BP/SF-1 and GCNF, aligned in tandem (2005)
  • Author(s): S. Ishihara, K. Morohashi
  • Journal Title: Biochem. Biophys. Res. Commun. 329 Pages: 554-562
• [Journal Article] Expression of the estrogen-inducible EGFP gene in aromatase-deficient mice reveals differential tissue-response to estrogenic compounds (2005)
  • Author(s): K. Toda, Y. Hayashi, T. Okada, K. Morohashi, T. Saibara
  • Journal Title: Mol. Cell. Endocrinol. 229 Pages: 119-126
• [Journal Article] Identification of the boundary for histone acetylation between nuclear receptor genes, Ad4BP/SF-1 and GCNF, aligned in tandem. (2005)
  • Author(s): Satoru Ishihara, Ken-ichirou Morohashi et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 329 Pages: 554-562
• [Journal Article] VMH Specific Enhancer of Ad4BP/SF-1 Gene. (2005)
  • Author(s): Yuichi Shima, Ken-ichirou Morohashi et al.
  • Journal Title: Mol.Endocrinol. 19 Pages: 2812-2823
• [Journal Article] Mesonephric FGF signaling is associated with the development of sexually indifferent gonadal primordium in chick embryos. (2005)
  • Author(s): Hidefumi Yoshioka, Ken-ichirou Morohashi et al.
  • Journal Title: Dev.Biol. 280 Pages: 150-161
• [Journal Article] A Novel Isoform of Vinexin, Vinexin g, Regulates Sox9 Gene Expression through activation of MAPK Cascade in Mouse Fetal Gonad. (2005)
  • Author(s): Makoto Matsuyama, Ken-ichirou Morohashi et al.
  • Journal Title: Genes to Cells 10 Pages: 421-434
• [Journal Article] Mouse Polycomb M33 is required for splenic vascular and adrenal gland formation through regulating Ad4BP/SF-1 expression. (2005)
  • Author(s): Yuko Katoh-Fukui, Ken-ichirou Morohashi et al.
  • Journal Title: Blood 106 Pages: 1612-1620
• [Journal Article] Ah (dioxin) receptor as a key factor in the regulation of female reproduction. (2005)
  • Author(s): Takashi Baba, Ken-ichirou Morohashi^*, Yoshiaki Fujii-Kuriyama^* et al.
  • Journal Title: Mol.Cell.Biol. 25 Pages: 10040-100511
• [Journal Article] Mesonephric Fgf9 as the signal that stimulates gonad formation. (2005)
  • Author(s): Hidefumi Yoshioka et al.
  • Journal Title: Dev.Biol. 280 Pages: 150-161
• [Journal Article] Identification of the boundary for histone acetylation between nuclear receptor genes, Ad4BP/SF-1 and GCNF, aligned in tandem. (2005)
  • Author(s): Satoru Ishihara, Ken-ichirou Morohashi
  • Journal Title: Biochem.Biophys.Res.Commun. 329 Pages: 554-562
• [Journal Article] Novel Isoform of Vinexin, Vinexinγ, Regulates Sox9 Gene Expression through activating MAPK Cascade in Mouse Fetal Gonad (2005)
  • Author(s): Makoto Matsuyama et al.
  • Journal Title: Genes to Cells (In press)
• [Journal Article] Testicular dysgenesis without adrenal insufficiency in a 46, XY patient with a heterozygous inactive mutation of Steroidogenic Factor-1 (2004)
  • Author(s): T. Hasegawa, M. Fukami, N. Sato, G. Sasaki, K. Fukutani, K. Morohashi, T. Ogata
  • Journal Title: J. Clin. Endocrinol. Metab. 89 Pages: 5930-5935
• [Journal Article] SUMO-1 modification of the synergy control motif of Ad4BP/SF-1 regulates synergistic transcription between Ad4BP/SF-1 and Sox9 (2004)
  • Author(s): T. Komatsu, H. Mizusaki, T. Mukai, H. Ogawa, D. Baba, M. Shirakawa, S. Hatakeyama, K. Nakayama, H. Yamamoto, A. Kikuchi, K. Morohashi
  • Journal Title: Mol. Endocrinol. 18 Pages: 2451-2462
• [Journal Article] Nuclear structure-associated TIF2 interacts with glucocorticoid receptor and its target DNA (2004)
  • Author(s): H. Ogawa, R.T. Yu, T. Haraguchi, Y. Hiraoka, Y. Nakatani, K. Morohashi, K. Umesono
  • Journal Title: Biochem. Biophys. Res Commun. 320 Pages: 218-225
• [Journal Article] Loss of PGC-specific expression of the orphan nuclear receptor ERR-b results in regulation of germ cell number in mouse embryos (2004)
  • Author(s): K. Mitsunaga, K. Araki, H. Mizusaki, K. Morohashi, H. Haruna, N. Nakagata, V. Giguere, K. Yamamura, K. Abe
  • Journal Title: Mechanism of Development 121 Pages: 237-246
• [Journal Article] Aromatase-knockout mouse carrying an estrogeninducible enhanced green fluorescent protein gene facilitates detection of estrogen actions in vivo (2004)
  • Author(s): K. Toda, Y. Okada, M. Zubair, K. Morohashi, T. Saibara, T. Okada
  • Journal Title: Endocrinol. 145 Pages: 1880-1888
• [Journal Article] Mutations of ARX are associated with striking pleiotropy and consistent genotype-phenotype correlation (2004)
  • Author(s): M. Kato, S. Das, K. Petras, K. Kitamura, K.Morohashi, D. N. Abuelo, M. Barr, D. Bonneau,A. Brady, N. J. Carpenter, F. Frisone, T.Fukuda, R.Guerrini, E. Iida, M. Itoh, A. F. Lewanda, Y.Nanba, A. Oka, V. K. Proud, K. L. Russel, P.Saugier-Veber, S. L. Schelley, A. Selicorni, R.Shaner, M. Silengo, F. Stewart, N. Sugiyama, J. Toyama, A. Toutain, A. L. Vargas, M. Yanazawa, E. H. Zackai, W. B. Dobyns
  • Journal Title: Human Mutation 23 Pages: 147-159
• [Journal Article] SUMO-1 Modification of the Synergy Control Motif of Ad4BP/SF-1 Regulates Synergistic Transcription between Ad4BP/SF-1 and Sox9. (2004)
  • Author(s): Tomoko Komatsu et al.
  • Journal Title: Mol.Endocrinol. 18 Pages: 2451-2462
• [Journal Article] Aromatase-knockout mouse carrying an estrogen-inducible enhanced green fluorescent protein gene facilitates detection of estrogen actions in vivo. (2004)
  • Author(s): Katsumi Toda et al.
  • Journal Title: Endocrinol. 145 Pages: 1880-1888
• [Journal Article] Nuclear structure-associated TIF2 interacts with glucocorticoid receptor and its target DNA. (2004)
  • Author(s): Hidesato Ogawa et al.
  • Journal Title: Biochem.Biophys.Res Commun. 320 Pages: 218-225
• [Presentation] 生殖腺ならびに副腎皮質特異的エンハンサーと細胞分化のメカニズム (2010)
  • Author(s): 諸橋憲一郎
  • Organizer: 第3回 生殖・発達内分泌研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-14
• [Presentation] ステロイドホルモン産生組織の分化メカニズム (2010)
  • Author(s): 諸橋憲一郎
  • Organizer: 第30回 北海道小児内分泌研究会
  • Place of Presentation: 札幌
• [Presentation] Genetic Program of Gonad Differentiation (2010)
  • Author(s): 諸橋憲一郎
  • Organizer: 第73回 日本循環器学会
  • Place of Presentation: 大阪
• [Presentation] ダイオキシン受容体(AhR)の生殖腺における機能:性ステロイドホルモンの合成 (2009)
  • Author(s): 諸橋憲一郎
  • Organizer: 第9回 Endocrine Forum on Women's Health
  • Place of Presentation: 青森
  • Year and Date: 2009-05-17
• [Presentation] Purification and analysis of a factor interacting with SUMOylated Ad4BP/SF-1 (2009)
  • Author(s): K.Morohashi
  • Organizer: 13th Adrenal Cortex Conference
  • Place of Presentation: San Francisco, USA
• [Presentation] From Tissue Specific Enhancers to Mechanism of Tissue Differentiation (2009)
  • Author(s): K.Morohashi, et al.
  • Organizer: International Symposium for Sex Differentiation
  • Place of Presentation: Fukuoka, Japan
• [Presentation] CELL LINEAGE ANALYSIS DEMONSTRATES A DEVELOPMENTAL LINK BETWEEN MOUSE FETAL AND ADULT ADRENAL CELLS (2009)
  • Author(s): K.Morohashi, et al.
  • Organizer: 13th International Congress of Endocrinology
  • Place of Presentation: Rio de Janeiro, Brazil
• [Presentation] クロマチン構造変換を介したSUMO化依存的な転写制御システムの解析 (2009)
  • Author(s): 小川英知、小松朋子、諸橋憲一郎
  • Organizer: 第31回日本分子生物学会,第81回日本生化学会合同年会
  • Place of Presentation: 神戸
• [Presentation] Abnormal epithelial cell polarity and migration of Emx2 KO embryonic gonads induced by ectopic EGFR expression (2009)
  • Author(s): Masatomo Kusaka, et al.
  • Organizer: 第41回 日本発生生物学会
  • Place of Presentation: 徳島
• [Presentation] Genetic Program of Gonad Development and Gonad Sex Differentiation (2009)
  • Author(s): 諸橋憲一郎
  • Organizer: 第85回 日本生理学会
  • Place of Presentation: 東京
• [Presentation] Enx2 regulates coelomic epithelial cell polarity via regulation of EGFR (2007)
  • Author(s): 日下 雅友
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] SUMO化依存的なクロマチン構造変換を介した転写制御システムの解析 (2007)
  • Author(s): 小川 英知
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] ニワトリ性決定遺伝子の探索 (2007)
  • Author(s): 石丸 善康
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] Molecular Mechanism of Ad4BP/SF-1 on Sex Differntiation (2007)
  • Author(s): 馬場 崇
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] Molecular Mechanism of Ad4BP/SF-1 on Sex Differntiation (2007)
  • Author(s): 諸橋 憲一郎
  • Organizer: International Conference of Molecular Biology of Life Sciences 2007
  • Place of Presentation: Convention Hall of Widyaloka Brawijaya University Malang, Indonesia
  • Year and Date: 2007-11-19
• [Presentation] Molecular mechanism of sex differentiation (2007)
  • Author(s): K. Morohashi
  • Organizer: International Conference on Molecular Biology of Life Sciences
  • Place of Presentation: Malang, Indonesia (Plenary Session)
• [Presentation] Medal Lecture From Ad4BP/SF-1 to cell and tissue differentiation (2007)
  • Author(s): K. Morohashi
  • Organizer: Society for Endocrinology BES
  • Place of Presentation: Birmingham, UK, Asia and Oceania
• [Presentation] Function of AhR in the Ovary (2006)
  • Author(s): K. Morohashi
  • Organizer: International Symposium on the Environmental Risks of Chemicals
  • Place of Presentation: Kushiro, Japan
• [Presentation] Adrenocortical Development Cell lineage analysis demonstrates a developmental link between mouse fetal and adult adrenal cells
  • Author(s): M. Zubair, K. L. Parker, K. Morohashi
  • Organizer: 13th International Congress of Endocrinology
  • Place of Presentation: Rio de Janeiro, Brazil Symposium
• [Presentation] From Tissue Specific Enhancers to Mechanism of Tissue Differentiation
  • Author(s): Y. Shima, K. Miyabayashi, M. Zubair, K. Morohashi
  • Organizer: International Symposium for Sex Differentiation
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Purification and analysis of a factor interacting with SUMOylated Ad4BP/SF-1
  • Author(s): K. Morohashi
  • Organizer: 13th Adrenal Cortex Conference
  • Place of Presentation: San Francisco, USA
• [Presentation] Regulation of Ad4BP/SF-1 by SUMO
  • Author(s): T. Komatsu, H. Ogawa, Morohashi K
  • Organizer: The 4th International Nuclear Receptor Meeting in Japan
  • Place of Presentation: Osaka, Japan
• [Presentation] Genetic program of gonad differentiation
  • Author(s): K. Morohashi, Y. Ishimaru, M. Zubair, S. Oka, K. Miyabayashi, M. Kusaka, Y. Shima, T. Baba, H. Ogawa, H. Yoshioka, Y. Katoh-Fukui
  • Organizer: 16th Lake Shirakaba Conference
  • Place of Presentation: Grenada
• [Presentation] Regulation of Ad4BP/SF-1 by SUMO
  • Author(s): T. Komatsu, M. Tsuchiya, H. Ogawa, K. Morohashi
  • Organizer: The Fourth NIBB-EMBL Symposium Biology of Protein Conjugation-Structure and Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Mechanism for asymmetric gonad development in birds
  • Author(s): H. Yoshioka, Y. Ishimaru, T. Komatsu, M. Kasahara, Y. Katoh-Fukui, K. Morohashi
  • Organizer: International Symposium on "Molecular Mechanisms of Sex Determination and Differentiation" at the 77th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: Matsue, Japan
• [Presentation] Queensland, Australia Mechanism for asymmetric development of the avian ovary
  • Author(s): K. Morohashi
  • Organizer: Special developmental biology seminar
  • Place of Presentation: Institute for Molecular Bioscience, Univ.
• [Presentation] Two-step regulation of Ad4BP/SF-1 gene transcription during fetal adrenal development
  • Author(s): K. Morohashi
  • Organizer: 12th Adrenal Cortex and 5th Molecular Steroidogenesis Conference
  • Place of Presentation: Boston, USA
• [Presentation] Invited Speaker Regulation of orphan nuclear receptor, Ad4BP/SF-1, by SUMO
  • Author(s): Komatsu T, Tsuchiya M, Ogawa H, Morohashi, K.
  • Organizer: 17th International Symposium of the Journal of Steroid Biochemistry and Molecular Biology
  • Place of Presentation: Seefeld, Austria
• [Presentation] Transcriptional Regulation of Genes implicated in Gonad Development
  • Author(s): K. Morohashi, M. Kusaka, K. Miyabayashi, Y. Ishimaru, Z. Mohamad, H. Ogawa, H. Yoshioka, Y. Katoh-Fukui
  • Organizer: Fourth International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Hawaii, USA (Opening Lecture)
• [Presentation] Genetic Program of Gonad Differentiation
  • Author(s): K. Morohashi, Y. Ishimaru, M. Zubair, S. Oka, K. Miyabayashi, M. Kusaka, Y. Shima, T. Baba, H. Ogawa, H. Yoshioka, Y. Katho-Fukui
  • Organizer: The 52nd NIBB conference, Reproductive Strategies
  • Place of Presentation: Okazaki, Japan
• [Presentation] Transcription factors in gonadal sex differentiation
  • Author(s): K. Morohashi
  • Organizer: The 12th International Congress of Endocrinology
  • Place of Presentation: Lisbon Portugal
• [Presentation] Growth factors from mesonephros implicated in gonadal and adrenal differentiation
  • Author(s): K. Morohashi, Y. Ishimaru, N. Sugiyama, H. Yoshioka
  • Organizer: The 11th Adrenal Cortex Conference
  • Place of Presentation: New Orleans, UAS
• [Presentation] Ah (dioxin) Receptor as a Key Factor in the Regulation of Female Reproduction
  • Author(s): Morohashi K, Baba T, Miura J, Nakamura N, Harada N, Yamamoto M, Fujii-Kuriyama
  • Organizer: The 3rd International Nuclear Receptor Meeting in Japan
  • Place of Presentation: Osaka, Japan
• [Presentation] シンポジウム:循環器領域の性差医療の展開Genetic Program of Gonad Differentiation
  • Author(s): 諸橋憲一郎
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪
• [Presentation] 生殖腺ならびに副腎皮質特異的エンハンサーと細胞分化のメカニズム
  • Author(s): 諸橋憲一郎
  • Organizer: 第3回生殖・発達内分泌研究会基調講演
  • Place of Presentation: 東京
• [Presentation] 特別講演ステロイドホルモン産生組織の分化メカニズム
  • Author(s): 諸橋憲一郎
  • Organizer: 第30回北海道小児内分泌研究会
  • Place of Presentation: 札幌
• [Presentation] シンポジウム:核内受容体研究の最前線クロマチン構造変換を介したSUMO化依存的な転写制御システムの解析
  • Author(s): 小川英知、小松朋子、諸橋憲一郎
  • Organizer: 第31回日本分子生物学会、第81回日本生化学会合同年会
  • Place of Presentation: 神戸
• [Presentation] ダイオキシン受容体(AhR)の生殖腺における機能:性ステロイドホルモンの合成
  • Author(s): 馬場崇、藤井義明、諸橋憲一郎
  • Organizer: 第9回Endocrine Forum on Women's Health
  • Place of Presentation: 青森
• [Presentation] ワークショップReproduction & Gametology Abnormal epithelial cell polarity and migration of Emx2 KO embryonic gonads induced by ectopic EGFR expression
  • Author(s): M. Kusaka, Y. Katoh-Fukui, H. Ogawa, K. Miyabayashi, T. Baba, N. Sugiyama, Y. Sugimoto, Y. Okuno, R. Kodama, A. Iizuka-Kogo, T. Senda, S. Aizawa, K. Morohashi
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島
• [Presentation] シンポジウム:性差の分子メカニズムGenetic Program of Gonad Development and Gonad Sex Differentiation
  • Author(s): 諸橋憲一郎
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
• [Presentation] SUMO化依存的なクロマチン構造変換を介した転写制御システムの解析
  • Author(s): 小川英知、小松朋子、諸橋憲一郎
  • Organizer: 第30回日本分子生物学会、第80回日本生化学会
  • Place of Presentation: 横浜
• [Presentation] シンポジウム:循環器領域における性差医療「性分化の分子メカニズム」
  • Author(s): 諸橋憲一郎
  • Organizer: 第55回日本心臓病学会
  • Place of Presentation: 東京
• [Presentation] シンポジウム転写因子ネットワーク「脳下垂体、副腎、視床下部、生殖腺特異的転写制御の実体」
  • Author(s): 諸橋憲一郎、嶋雄一、モハマドズバイル、岡早苗、福井由宇子
  • Organizer: 第22回脳下垂体研究会
  • Place of Presentation: 湘南国際村センター
• [Presentation] 招待講演「性の不思議」性分化機構の解明に向けて
  • Author(s): 諸橋憲一郎
  • Organizer: 第164回生命科学フォーラム
  • Place of Presentation: 東京
• [Presentation] ステロイド産生組織の形成機構
  • Author(s): 諸橋憲一郎、モハマドズバイル、福井由宇子、石丸善康、吉岡秀文
  • Organizer: 第1回博多シンポジウム「内・外環境と生物応答」
  • Place of Presentation: 福岡
• [Presentation] ちょっと変わったニワトリの卵巣
  • Author(s): 諸橋憲一郎
  • Organizer: 第7回Women's Health Forum
  • Place of Presentation: 神戸
• [Presentation] シンポジウム:SUMO化とユビキチン化による遺伝子転写因子活性化機構SUMO化修飾を介した転写因子のクロマチン制御機構
  • Author(s): 小川英知、小松朋子、諸橋憲一郎
  • Organizer: 第79回日本内分泌学会学術総会
  • Place of Presentation: 神戸
• [Presentation] 性と性腺の分化の遺伝子制御
  • Author(s): 諸橋憲一郎
  • Organizer: 第10回日研シンポジウム「生命科学の最前線」
  • Place of Presentation: 福岡
• [Presentation] オーガナイザー性を決める役者(遺伝子)たち
  • Author(s): 諸橋憲一郎
  • Organizer: 特定領域研究市民公開シンポジウム「性の不思議」
  • Place of Presentation: 東京
• [Presentation] 教育講演「性決定の分子メカニズム」
  • Author(s): 諸橋憲一郎
  • Organizer: 第78回日本内分泌学会学術総会
  • Place of Presentation: 東京
• [Presentation] シンポジウム「核内受容体によるホルモン作用の新展開」Ad4BP/SF-1のSUMO化による転写調節
  • Author(s): 諸橋憲一郎、小松朋子、小川英知
  • Organizer: 第78回日本内分泌学会学術総会
  • Place of Presentation: 東京
• [Presentation] ワークショップ「核内レセプター研究の最前線」Ad4BP/SF-1遺伝子の組織特異的発現制御機構
  • Author(s): 諸橋憲一郎、モハマドズバイル、嶋雄一、日下雅友、杉山紀之、小川英知、福井由宇子
  • Organizer: 第27回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] 招請講演:性分化の分子メカニズム
  • Author(s): 諸橋憲一郎
  • Organizer: 第9回日本生殖内分泌学会
  • Place of Presentation: 大阪
• [Presentation] 転写群因子による生殖腺の分化、及び性分化制御機構
  • Author(s): 諸橋憲一郎
  • Organizer: 第1回特定領域「性分化機構の解明」シンポジウム
  • Place of Presentation: 東京
• [Presentation] シンポジウム:内分泌代謝学におけるシグナル伝達と臨床応用生殖腺の分化(性分化)メカニズム
  • Author(s): 諸橋憲一郎、吉岡秀文、福井由宇子
  • Organizer: 第77回日本内分泌学会
  • Place of Presentation: 京都
• [Presentation] 卵巣機能とダイオキシン
  • Author(s): 諸橋憲一郎
  • Organizer: 第5回Women's Health Forum
  • Place of Presentation: 京都
• [Presentation] 教育講演:性分化の分子メカニズム
  • Author(s): 諸橋憲一郎
  • Organizer: 第3回ステロイドホルモンを考える会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/seisaseibutu/
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/seisaseibutu/
• [Remarks]
  • URL: http://www.nibb.ac.jp/seibunka/index.html