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X-ray Crystallographic Studies on Mechanism of Photosystem II Membrane Protein Complex

Research Project

Project/Area Number 16087102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka City University (2005-2009)
The Institute of Physical and Chemical Research (2004)

Principal Investigator

KAMIYA Nobuo  Osaka City University, 大学院・理学研究科, 教授 (60152865)

Co-Investigator(Kenkyū-buntansha) SHIN Kenjin  岡山大学, 理学部, 教授 (60261161)
IKEUCHI Akihiko  東京大学, 大学院・総合文化研究科, 教授 (20159601)
NAKAZATO Katsuyoshi  日本大学, 文理学部, 准教授 (50172292)
宮原 郁子  大阪市立大学, 大学院・理学研究科, 准教授 (40271176)
逸見 隆博  大阪市立大学, 大学院理学研究科, 博士研究員 (30419692)
内藤 久志  独立行政法人理化学研究所, 横山構造分子生物学研究室, 先任研究員 (40392047)
古瀬 宗則  独立行政法人理化学研究所, 三木生物超分子結晶学研究室, 協力研究員 (50321807)
Co-Investigator(Renkei-kenkyūsha) UMENA Yasufumi  大阪市立大学, 大学院・理学研究科, 博士研究員 (10468267)
Project Period (FY) 2004 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥82,800,000 (Direct Cost: ¥82,800,000)
Fiscal Year 2009: ¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2008: ¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2007: ¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2006: ¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2005: ¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2004: ¥13,500,000 (Direct Cost: ¥13,500,000)
Keywords光合成 / X線結晶構造解析 / 膜蛋白質 / 超分子 / 光エネルギー変換 / 酸素発生 / 光工ネルギー変換 / 形質転換法 / 膜タンパク質 / 低分子量サブユニット欠失変異体 / 精製 / 結晶化 / 溶解度曲線
Research Abstract

Photosystem II (PSII) catalyzes the oxygen evolution from water in the photosynthesis utilizing solar light energy. We succeeded to determine, first in the world, the three-dimensional structure of the oxygen-evolving Mn4Ca cluster at atomic resolution, including the whole structure of PSII. Because PSII also provides chemical energy for CO2 condensation, our results serve structural strict-bases for efficient use of solar energy in the green chemistry.

Report

(7 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report

Research Products

(76 results)

All 2010 2009 2008 2007 2006 2005 2004 1994 Other

All Journal Article (46 results) (of which Peer Reviewed: 30 results) Presentation (21 results) Book (6 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Structural and functional studies on Ycf12 (Psb30) and PsbZ deletion mutants from a thermophilic cyanobacterium2010

    • Author(s)
      Takasaka, K., Iwai, M., Umena, Y., Kawakami, K., Ikeuchi, M., Kamiya, N., Shen, J. -R.
    • Journal Title

      BBA 1797

      Pages: 278-284

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structura an nctiona studies on Ycf12 (Psb30) and PsbZ deletion mutants from a thermophilic cyanobacterium2010

    • Author(s)
      Takasaka, K., Iwai, M., Umena, Y., Kawakami, K., Ikeuchi, M., Kamiya, N., Shen, J.-R.
    • Journal Title

      BBA 1797

      Pages: 278-284

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Location of chloride and its possible functions in oxygen-evolving photosystem II revealed by X-ray crystallography2009

    • Author(s)
      Kawakami, K., Umena, Y., Kamiya, N., Shen, J. -R.
    • Journal Title

      PNAS 106

      Pages: 8567-8572

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Towards structural elucidation of eukaryotic photosystem II: Purification, crystallization and preliminary X-ray diffraction analysis of photosystem II from a red alga2009

    • Author(s)
      Adachi, H., Umena, Y., Enami, I., Henmi, T., Kamiya, N., Shen, J. -R.
    • Journal Title

      BBA 1787

      Pages: 121-128

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Is the photosystem II complex a monomer or a dimer?2009

    • Author(s)
      Watanabe, M., Iwai, M., Narikawa, R., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 1674-1680

    • NAID

      10027343285

    • Related Report
      2009 Annual Research Report 2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Location of chloride and its possible functions in oxygen-evolving photosystem II revealed by X-ray crystallography2009

    • Author(s)
      Kawakami, K., Umena, Y., Kamiya, N., Shen, J.-R.
    • Journal Title

      PNAS 106

      Pages: 8567-8572

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Towards structural elucidation of eukaryotic photosystem II : Purification, crystallization and preliminary X-ray diffraction analysis of photosystem II from a red alga2009

    • Author(s)
      Adachi, H., Umena, Y., Enami, I., Henmi, T., Kamiya, N., Shen, J.-R.
    • Journal Title

      BBA 1787

      Pages: 121-128

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Towards structural elucidation of eukaryotic photosystem II : Purification, crystallization and preliminary X-rav diffraction analysis of nhotosystem II from a red alga2009

    • Author(s)
      H. Adachi, Y. Umena, I. Enami, T. Henmi, N. Kamiya, J.-R. Shen
    • Journal Title

      Biochim. Biophys. Acta 1787

      Pages: 121-128

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystallographic and biochemical characterizations of a mutant Photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation2008

    • Author(s)
      T. Henmi, M. Iwai, M. Ikeuchi, K. Kawakami, J. -R. Shen, N. Kamiya
    • Journal Title

      J. Synchrotron Rad. 15

      Pages: 304-307

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 2, 2 ' -b-hydroxylase is involved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2 ' -fucoside in Thermosynechococcus elongatus strain BP-1.2008

    • Author(s)
      Iwai, M., Maoka, T., Ikeuchi, M., Takaichi, S.
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 1678-1687

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structural analysis of photosystem II complex with the novel method to reduce X-ray radiation damage2008

    • Author(s)
      A. Okumura, R. Nagao, T. Suzuki, S. Yamagoe, M. Iwai, K. Nakazato, I. Enami
    • Journal Title

      BBA 1777

      Pages: 1545-1551

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystallographic and biochemical characterizations of a mutant Photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation2008

    • Author(s)
      T. Henmi, M. Iwai, M. Ikeuchi, K. Kawakami, J.-R. Shen, N. Kamiya
    • Journal Title

      J. Synchrotron Rad. 15

      Pages: 304-307

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2, 2'-b-hydroxylase is involved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2'-fucoside in Thermosvnechococcus elonzatus strain BP-12008

    • Author(s)
      Iwai, M., Maoka, T., Ikeuchi, M. and Takaichi, S
    • Journal Title

      Plant Cell Physiology 49

      Pages: 1678-1687

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel protein in Photosystem II of a diatom Chaetoceros gracilis is one of the extrinsic proteins located on lumenal side and directly associates with PSII core comnonents2008

    • Author(s)
      A. Okumura, R. Nagao, T. Suzuki, S. Yamagoe, M. Iwai, K. Nakazato, I. Enami
    • Journal Title

      Biochimica et Biophysica Acta-Bioenergetics 1777

      Pages: 1545-1551

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystallographic and biochemical characterizations of a mutant Photogyste m II complex from Thermosynechococcus vulcanus wit5h the psbTc gene inactivated by an insertion mutation2008

    • Author(s)
      Takahiro Henmi, Masako Iwai, Masahiko Ikeuchi, Keisuke Kawakami, Jian-Ren Shen, Nobuo Kamiya
    • Journal Title

      J. Synchrotron Rad. 15

      Pages: 304-307

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography2007

    • Author(s)
      Keisuke Kawakami, Masako Iwai, Masahiko Ikeuchi, Nobuo Kamiya, Jian-Ren Shen
    • Journal Title

      FEBS Lett. 581

      Pages: 4983-4987

    • NAID

      130006994394

    • Related Report
      2009 Final Research Report 2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning2007

    • Author(s)
      Akinori Okumura, Masanori Sano, Takehiro Suzuki, Hiroyasu Tanaka, Ryo Nagao, Katsuyoshi Nakazato, Masako Iwai, Hideyuki Adachi, Jian-Ren Shen, Isao Enami
    • Journal Title

      FEBS Lett. 581

      Pages: 5255-5258

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for is functioning2007

    • Author(s)
      Akinori Okumura, Masanori Sano, Takehiro Suzuki, Hiroyasu Tanaka, Ryo Nagao, Katsuyoshi Nakazato, Masako Iwai, Hideyuki Adachi, Jian-Ren Shen and Isao Enami
    • Journal Title

      FEBS Lett. 581

      Pages: 5255-5258

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] g-Anisotropy of the S2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy2006

    • Author(s)
      Matsuoka H., Furukawa K., Kato T., Mino H., Shen J. -R., Kawamori A.
    • Journal Title

      J. Phys. Chem. B. 110

      Pages: 13242-13247

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipids in Oxygen-Evolving Photosystem II Complexes of Cyanobacteria and Higher Plants2006

    • Author(s)
      Sakurai I., Shen J. -R., Leng J., Ohashi S., Kobayashi M., Wada H.
    • Journal Title

      J. Biochem. (Tokyo) 140

      Pages: 201-209

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Absence of the psbH gene product destabilizes Photosystem II complex: the lack of PSII-X protein in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-12006

    • Author(s)
      Iwai, M., Katayama, M., Ikeuchi, M.
    • Journal Title

      Photosynth. Res. 87

      Pages: 313-322

    • NAID

      130006988520

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutations in a putative chloride efflux transporter gene suppress the chloride requirement of the photosystem II in pdbV-disruptant (or cytochrome c550 deficient mutant)2006

    • Author(s)
      Kobayashi, M., Katoh, H., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 47

      Pages: 799-804

    • Related Report
      2009 Final Research Report 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] g-Anisotropy of the S_2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy2006

    • Author(s)
      Matsuoka H., Furukawa K., Kato T., Mino H., Shen J.-R., Kawamori A.
    • Journal Title

      J. Phys. Chem. B. 110

      Pages: 13242-13247

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lipids in Oxygen-Evolving Photosystem II Complexes of Cyanobacteria and Higher Plants2006

    • Author(s)
      Sakurai I., Shen J.-R., Leng J., Ohashi S., Kobayashi M., Wada H.
    • Journal Title

      J. Biochem. (Tokyo) 140

      Pages: 201-209

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Absence of the psbH gene product destabilizes Photosystem II complex : the lack of PSII-X protein in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-12006

    • Author(s)
      Iwai, M., Katayama, M., Ikeuchi, M.
    • Journal Title

      Photosynth. Res. 87

      Pages: 313-322

    • NAID

      130006988520

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pressure Equilibrium and Jump Study on Unfolding of 23kDa Protein from Spinach Photosystem II2005

    • Author(s)
      Tan C. -Y., Xu C. -H., Wong J., Shen J. -R., Sakuma S., Yamamoto Y., Lange R., Balny C., Ruan K. -C.
    • Journal Title

      Biophys J. 88

      Pages: 1264-1275

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Key cofactors of photosystem II cores from four organisms identified by 1. 7-K absorption2005

    • Author(s)
      Arskold S.P., Smith P.J., Shen J. -R., Pace R.J., Krausz E.
    • Journal Title

      CD and MCD, Photosynthesis Res. 84

      Pages: 309-316

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II2005

    • Author(s)
      Enami I., Suzuki T., Tada O., Nakada Y., Nakamura K., Tohri A., Ohta H., Inoue I., Shen J. -R.
    • Journal Title

      FEBS Journal 272

      Pages: 983-990

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of photosystem II: An FtsH protease plays an essential role in the turnover of the reaction center D1 protein in Synechocystis PCC 6803 under heat stress as well as light stress conditions2005

    • Author(s)
      Kamata T., Hiramoto H., Morita N., Shen J. -R., Mann N.H., Yamamoto Y.
    • Journal Title

      Photochemical & Photobiological Sciences 4

      Pages: 83-990

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pigment exchange of Phosystem II reaction center by chlorophyll d2005

    • Author(s)
      Tomo T., Hirano E., Nagata J., Nakazato K.
    • Journal Title

      Photosynthesis Research 84

      Pages: 77-83

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 光化学系II複合体の構造と機能2005

    • Author(s)
      神谷信夫, 沈建仁
    • Journal Title

      蛋白質核酸酵素 50

      Pages: 1174-1179

    • Related Report
      2009 Final Research Report
  • [Journal Article] Pressure Equilibrium and Jump Study on Unfolding of 23kDa Protein from Spinach Photosystem II2005

    • Author(s)
      Tan C.-Y., Xu C.-H., Wong J., Shen J.-R., Sakuma S., Yamamoto Y., Lange R., Balny C., Ruan K.-C.
    • Journal Title

      Biophys J. 88

      Pages: 1264-1275

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Key cofactors of photosystem II sores_from_four organisms identified by 1.7-K absorption, CD and MCD2005

    • Author(s)
      Arskold S.P., Smith P.J., Shen J.-R., Pace R.J., Krausz E.
    • Journal Title

      Photosynthesis Res. 84

      Pages: 309-316

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II2005

    • Author(s)
      Enami I., Suzuki T., Tada 0., Nakada Y., Nakamura K., Tohri A., Ohta H., Inoue I., Shen J.-R.
    • Journal Title

      FEBS Journal 272

      Pages: 5020-5030

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Quality control of photosystem II : An FtsH protease plays an essential role in the turnover of the reaction center D1 protein in Synechocystis PCC 6803 under heat stress as well as light stress conditions2005

    • Author(s)
      Kamata T., Hiramoto H., Morita N., Shen J.-R., Mann N.H., Yamamoto Y.
    • Journal Title

      Photochemical & Photobiological Sciences 4

      Pages: 983-990

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pigment exchange of Phosystem_II reaction center by chlorophyll d2005

    • Author(s)
      Tomo T., Hirano E., Nagata J., Nakazato K.
    • Journal Title

      Potosynthesis Research 84

      Pages: 77-83

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 光化学系II複合体の構造と機能2005

    • Author(s)
      神谷 信夫, 沈 建仁
    • Journal Title

      蛋白質 核酸 酵素 50(10)

      Pages: 1174-1179

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pressure equilibrium and jump study on unfolding of 23 kDa protein from spinach photosystem II.2005

    • Author(s)
      Cui-Yan Tan, Chun-He Xu, Jun Wong, Jian-Ren Shen, et al.
    • Journal Title

      Biophys.J. (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Improved genetic transformation of the thermophilic cyanobacterium2004

    • Author(s)
      Iwai, M., Katoh, H., Katayama, M., Ikeuchi, M.
    • Journal Title

      Thermosynechococcus elongatus BP-1, Plant Cell Physiol. 45

      Pages: 171-175

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Response to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis sp. PCC 68032004

    • Author(s)
      Kobayashi, M., Ishizuka, T., Katayama, M., Kanehisa, M., Bhattacharyya-Pakrasi, M., Pakrasi, H.B., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 290-299

    • NAID

      10012724002

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] PSII-Tc protein plays an important role in dimerization of Photosystem II.2004

    • Author(s)
      Iwai, M., Katoh, H., Katayama, M., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1809-1816

    • NAID

      10014214548

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-12004

    • Author(s)
      Iwai, M., Katoh, H., Katayama, M., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 171-175

    • NAID

      10012095796

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Response to Oxidative Stress Involves a Novel Peroxiredoxin Gene in the Unicellular Cyanobacterium Synechocystis sp.PCC 68032004

    • Author(s)
      Kobayashi, M., Ishizuka, T., Katayama, M., Kanehisa, M., Bhattacharyya-Pakrasi, M., Pakrasi, H.B., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 290-299

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Use of segment-based microarray in the analysis or global gene expression in response to various environmental stresses in the cyanobacterium Anabaena sp.PCC 71202004

    • Author(s)
      Sato, N., Ohmori, M., Ikeuchi, M., Tashiro, K., et al.
    • Journal Title

      J.Gen.Appl.Microbiol. 50

      Pages: 1-8

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular evolution of PAS domain-containing proteins of filamentous cyanobacteria through the domain shuffling and domain duplication.2004

    • Author(s)
      Narikawa, R., Okamoto, S., Ikeuchi, M., Ohmori, M.
    • Journal Title

      DNA Res. 11

      Pages: 69-81

    • Related Report
      2004 Annual Research Report
  • [Journal Article] PSII-Tc protein plays an important role in dimerization of Photosy stem II.2004

    • Author(s)
      Iwai, M., Katoh, H., Katayama, M, Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1809-1816

    • Related Report
      2004 Annual Research Report
  • [Presentation] Structure around Oxygen- Evolving Mn4OxCa-Cluster of Photosystem II Complex from Thermosynechococcus vulcanus2010

    • Author(s)
      N. Kamiya
    • Organizer
      2010 OCU International Symposium on the Foundation of Enbironmental Research
    • Place of Presentation
      Awaji, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] 光化学系II複合体結晶の分解能向上2010

    • Author(s)
      川上恵典、梅名泰史、田代隆慶、神谷信夫、沈建仁
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] 紅藻由来光化学系II複合体の精製と結晶の分解能の改善2010

    • Author(s)
      足立秀行、梅名泰史、田代隆慶、榎並勲、神谷信夫、沈建仁
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] 光化学系II膜蛋白質複合体の酸素発生中心の構造研究2009

    • Author(s)
      梅名泰史, 川上恵典, 大熊章郎, 西条慎也, 内藤久志, 沈建仁, 神谷信夫
    • Organizer
      日本結晶学会
    • Place of Presentation
      大坂
    • Related Report
      2009 Final Research Report
  • [Presentation] X線損傷低減データに基づく光化学系II複合体の酸素発生中心の構造研究2009

    • Author(s)
      梅名泰史・川上恵典・大熊章郎・西条慎也・内藤久志・沈建仁・神谷信夫
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] 光化学系II二量体の形成・安定化におけるPsbM, PsbIサブユニットの役割2009

    • Author(s)
      川上恵典, 岩井雅子, 川端洋輔, 逸見隆博, 池内昌彦, 神谷信夫, 沈建仁
    • Organizer
      第50回日本植物生理学会年会
    • Related Report
      2009 Final Research Report
  • [Presentation] Ycf12、PsbZ欠失変異体由来光化学系IIのX線結晶構造解析2009

    • Author(s)
      高坂賢之、岩井雅子、梅名泰史、神谷信夫、川上恵典、沈建仁
    • Organizer
      第50回日本植物生理学会年会
    • Related Report
      2009 Final Research Report
  • [Presentation] 光化学系II膜蛋白質複合体の酸素発生中心の構造研究2009

    • Author(s)
      梅名泰史, 川上恵典, 大熊章郎, 西条慎也, 内藤久志, 沈建仁, 神谷信夫
    • Organizer
      日本結晶学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Crystal structural analysis of photosystem II complex with the novel method to reduce X-ray radiation damage2008

    • Author(s)
      Y. Umena, K. Kawakami, A. Ohkuma, S. Saijyou, H. Naitow, J. -R. Shen, N. Kamiya
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Coordination structure and functional implications of two Cl-binding sites in oxygen-evolving photosystem II2008

    • Author(s)
      K. Kawakami, Y. Umena, N. Kamiya, J. -R. Shen
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Purification, crystallization and preliminary X-ray analysis of photosystem II dimer from a red alga Cyanidium caldarium2008

    • Author(s)
      H. Adachi, Y. Umena, I. Enami, N. Kamiya, J. -R. Shen
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] X線損傷低減データに基づく酸素発生光化学系II粒子の構造精密化2008

    • Author(s)
      梅名泰史, 川上恵典, 大熊章郎, 西条慎也, 内藤久志, 沈建仁, 神谷信夫
    • Organizer
      日本結晶学会2007年度年会
    • Related Report
      2009 Final Research Report
  • [Presentation] psbTc遺伝子を破壊した好熱性ラン藻変異株光化学系II複合体の結晶構造解析2008

    • Author(s)
      逸見隆博、岩井雅子、池内昌彦、沈建仁、神谷信夫
    • Organizer
      植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] 紅藻Cyanidium caldarium由来光化学系II複合体の精製・結晶化2008

    • Author(s)
      足立秀行、榎並勲、逸見隆博、神谷信夫、沈建仁
    • Organizer
      植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] PsbMサブユニットを欠失した光化学系II複合体の結晶構造解析2008

    • Author(s)
      川端洋輔、逸見隆博、岩井雅子、末益卓、川上恵典、青山智佳、池内昌彦、神谷信夫、沈建仁
    • Organizer
      植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] Crystal structural analysis of photosystem II complex with the novel method to reduce X-rav radiation damaae2008

    • Author(s)
      Y. Umena, K. Kawakami, A. Ohkuma, S. Saijyou, H. Naitow, J.-R. Shen, N. Kamiya
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] X-ray crystallorgraphic and biochemical characterizations od a mutant Photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation2007

    • Author(s)
      Takahiro Henmi
    • Organizer
      回折構造生物国際会議
    • Place of Presentation
      東京,日本
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Crystal Structure Analysis of a Mutant Photosystem II Complex from Thermosynechococcus vulcanus with the psbTc Gene Inactivated by an Insertion Mutation2007

    • Author(s)
      T. Henmi, M. Iwai, M. Ikeuchi, K. Kawakami, J. -R. Shen, N. Kamiya
    • Organizer
      The second International Symposium on Diffraction Structural Biology 2007
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Structural analysis of mutant Photosystem II complexes from Thermosynechococcus vulcanus2007

    • Author(s)
      K. Kawakami, Y. Kawabata, T. Henmi, M. Iwai, M. Ikeuchi, N. Kamiya, J. -R. Shen
    • Organizer
      The second International Symposium on Diffraction Structural Biology 2007
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Purification and crystallization of photosystem II complex from a red alga Cyanidium caldarium2007

    • Author(s)
      H. Adachi, I. Enami, T. Henmi, N. Kamiya, J. -R. Shen
    • Organizer
      The second International Symposium on Diffraction Structural Biology 2007
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] 紅藻Cyanidium caldarium光化学系II結晶分解能の改善とx線による分析

    • Author(s)
      足立秀行、梅名泰史、榎並勲、神谷信夫、沈建仁
    • Organizer
      第50回日本植物生理学会年会
    • Related Report
      2009 Final Research Report
  • [Book] A Structural Approach(Photosynthetic Protein Complexes)2008

    • Author(s)
      Shen J. -R., Henmi T., Kamiya N
    • Total Pages
      24
    • Publisher
      WILEY-VCH
    • Related Report
      2009 Final Research Report
  • [Book] Photosynthetic Protein Complexes, A Structural Approach2008

    • Author(s)
      Shen J.-R., Henmi T. and Kamiya N
    • Total Pages
      24
    • Publisher
      WILEY-VCH
    • Related Report
      2008 Annual Research Report
  • [Book] In Structure of Photosynthetic Proteins2008

    • Author(s)
      Shen J-R, Henmi T. and Kamiya N.
    • Total Pages
      15
    • Publisher
      Structure and Function of Photosystem II,In Structure of Photosynthetic Proteins
    • Related Report
      2007 Annual Research Report
  • [Book] The Light-Driven Water:Plastoquinone Oxidoreductase2005

    • Author(s)
      Shen J. -R., Kamiya N.
    • Total Pages
      29
    • Publisher
      Springer
    • Related Report
      2009 Final Research Report
  • [Book] Photosystem II : The Light-Driven Water : Plastoquinone Oxidoreductase2005

    • Author(s)
      Shen J.-R., Kamiya N.
    • Total Pages
      29
    • Publisher
      3D Crystal Structure of the Photosystem II Core
    • Related Report
      2005 Annual Research Report
  • [Book] Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants.(Methods Mol Biol., Carpentier R. (ed.))2004

    • Author(s)
      Jian-Ren Shen, Shinsuke Sakuma, Yasusi Yamamoto
    • Total Pages
      8
    • Publisher
      Humana Press
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質溶解度測定方法、結晶製造方法、及びそのための装置2008

    • Inventor(s)
      中里勝芳
    • Industrial Property Rights Holder
      中里勝芳
    • Acquisition Date
      2008-04-18
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 非イオン性界面活性剤自動除去装置2004

    • Inventor(s)
      中里勝芳
    • Industrial Property Rights Holder
      中里勝芳
    • Acquisition Date
      2004-06-18
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 非イオン性界面活性剤自動除去装置1994

    • Inventor(s)
      中里 勝芳
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      1994-06-30
    • Acquisition Date
      2004-06-18
    • Related Report
      2004 Annual Research Report

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Published: 2004-03-31   Modified: 2018-03-28  

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