Molecular mechanism of the functional regulation of the bacterial photosynthetic light-harvesting-reaction center complexes

• Project/Area Number: 16087201
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Ibaraki University (2005-2009); Tohoku University (2004)
• Principal Investigator: OTOMO Seiu (2005-2009) Ibaraki University, 理学部, 教授 (10213612); 野澤 庸則 (2004) 東北大学, 大学院・工学研究科, 教授 (10006322)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Masayuki 有明工業高等専門学校, 物質工学科, 准教授 (70271864) ; 大友 征宇 東北大学, 大学院・工学研究科, 助教授 (10213612)
• Project Period (FY): 2004 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥79,000,000 (Direct Cost: ¥79,000,000); Fiscal Year 2009: ¥4,100,000 (Direct Cost: ¥4,100,000); Fiscal Year 2008: ¥12,400,000 (Direct Cost: ¥12,400,000); Fiscal Year 2007: ¥15,100,000 (Direct Cost: ¥15,100,000); Fiscal Year 2006: ¥15,100,000 (Direct Cost: ¥15,100,000); Fiscal Year 2005: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2004: ¥15,700,000 (Direct Cost: ¥15,700,000)
• Keywords: 光合成 / 光捕集反応中心複合体 / 色素膜タンパク質 / 光電変換 / 膜タンパク質超分子複合体 / 結晶化 / LH1-RC複合体 / Thermochromatium tepidum / X線構造解析 / LH1 Qy遷移 / LHI Qy遷移 / LH1 Qy 遷移 / PufX / 超分子膜タンパク質 / デタージェント / 沈殿剤 / LH2

Research Abstract

The bacterial photosynthesis that provides a simplified model system ideally for studying the light energy conversion has been investigated using various physicochemical methods. We have determined the pigment geometry and structures of the polypeptides in an antenna structural subunit. A core light-harvesting-reaction center complex has been crystallized. The unusual spectroscopic behavior and enhanced thermostability of the core complex from a thennophilic bacterium has been clarified, revealing the adaptive strategy of the photosynthetic organism to survive in extreme environments using natural resources.

Research Products

• [Journal Article] Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum. (2009)
  • Author(s): Kimura, Y., Yu, L. -J., Hirano, Y., Suzuki, H., Wang, Z. -Y.
  • Journal Title: J. Biol. Chem. 284 Pages: 93-99
  • Peer Reviewed
• [Journal Article] Overexpression, characterization, and crystallization of the functional domain of cytochrome cz from Chlorobium tepidum. (2009)
  • Author(s): Higuchi, M., Hirano, Y., Kimura, Y., Oh-oka, H., Miki, K., Wang, Z. -Y.
  • Journal Title: Photosynth. Res. 102 Pages: 77-84
  • Peer Reviewed
• [Journal Article] Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling. (2009)
  • Author(s): Ma, F., Kimura, Y., Yu, L. -J., Wang, P., Ai, X. -C., Wang, Z. -Y., Zhang, J. -P.
  • Journal Title: FEBS Journal 276 Pages: 1739-1749
  • Peer Reviewed
• [Journal Article] Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum (2009)
  • Author(s): Y.Kimura, et al.
  • Journal Title: J.Biol.Chem. 284 Pages: 93-99
  • Peer Reviewed
• [Journal Article] Overexpression, characterization, and crystallization of the functional do main of cytochrome c_z from Chlorobium tepidum (2009)
  • Author(s): M.Higuchi, et al.
  • Journal Title: Photosynth.Res. 102 Pages: 77-84
  • Peer Reviewed
• [Journal Article] Overexpression, characterization, and crystallization of the functional do main of cytochrome c_z from Chlorobium tepidum (2009)
  • Author(s): F.Ma, et al.
  • Journal Title: FEBS J. 276 Pages: 1739-1749
  • Peer Reviewed
• [Journal Article] Calcium ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum (2009)
  • Author(s): Y. Kimura, et.al.
  • Journal Title: J. Biol. Chem. 284 Pages: 93-99
  • Peer Reviewed
• [Journal Article] Calcium ions are involved in the unusual red-shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purple sulfur bacterium Thermochromatium tepidum. (2008)
  • Author(s): Kimura, Y., Hirano, Y., Yu, L. -J., Suzuki, H., Kobayashi, M., Wang, Z. -Y.
  • Journal Title: J. Biol. Chem. 283 Pages: 13867-13873
  • Peer Reviewed
• [Journal Article] Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermo chromatium tepidum. (2008)
  • Author(s): Ma, F., Kimura, Y., Zhao, X. -H., Wu, Y. -S., Wang, P., Fu, L. -M., Wang, Z. -Y., Zhang, J. -P.
  • Journal Title: Biophys. J. 95 Pages: 3349-3357
  • Peer Reviewed
• [Journal Article] Calcium ions are involved in the unusual red-shift of the light-harvesting 1 Q_y transition of the core complex in thermophilic purple sulfur bacterium Thermochro matium tpnidum (2008)
  • Author(s): Y. Kimura, et.al.
  • Journal Title: J. Biol. Chem. 283 Pages: 13867-13873
  • Peer Reviewed
• [Journal Article] Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum (2008)
  • Author(s): F. Ma et.al.
  • Journal Title: Biophys. J. 95 Pages: 3349-3357
  • Peer Reviewed
• [Journal Article] Solution structure of the Rhodobacter sphaeroides PufX membrane protein: Implications for the quinone exchange and protein-protein interactions. (2007)
  • Author(s): Wang, Z. -Y., Suzuki, H., Kobayashi, M., Nozawa, T.
  • Journal Title: Biochemistry 46 Pages: 3635-3642
  • Peer Reviewed
• [Journal Article] Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum. (2007)
  • Author(s): Suzuki, H., Hirano, Y., Kimura, Y., Tataich, S., Kobayashi, M., Miki, K., Wang, Z. -Y.
  • Journal Title: Biochim. Biophys. Acta 1767 Pages: 1057-1063
  • Peer Reviewed
• [Journal Article] Overexpression and characterization of the Rhodobacter sphaeroides PufX membrane protein in Escherichia coli. (2007)
  • Author(s): Onodera, S., Suzuki, H., Shimada, Y., Kobayashi, M., Nozawa, T., Wang, Z. -Y.
  • Journal Title: Photochem. Photobiol. 83 Pages: 139-144
  • Peer Reviewed
• [Journal Article] solution structure of the Rhodobacter sphaeroides PufX membrane protein:Implications for the quinone exchange and protein-protein interactions (2007)
  • Author(s): Z.-Y, Wang, et. al.
  • Journal Title: Biochemistry 46 Pages: 3635-3642
  • Peer Reviewed
• [Journal Article] Purification,characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum (2007)
  • Author(s): H, Suzuk, et. al.
  • Journal Title: Biochim.Biophys.Acta 1767 Pages: 1057-1063
  • Peer Reviewed
• [Journal Article] Overexpression and characterization of the Rhodobacter sphaeroides PufXmembrane protein in Escherichia coli (2007)
  • Author(s): S, Onodera, et. al.
  • Journal Title: Photochem.Photobiol. 83 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Solution structure of the Rhodobacter sphaeroides PufX membrane protein : Implications for the quinone exchange and protein-protein interactions (2007)
  • Author(s): Z.-Y.Wang, et al.
  • Journal Title: Biochemistry 46 Pages: 3635-3642
• [Journal Article] Overexpression and characterization of the Rhodobacter sphaeroides PufX membrane protein in Escherichia coli (2007)
  • Author(s): Onodera, et al.
  • Journal Title: Photochem.Photobiol. 83 Pages: 139-144
• [Journal Article] Solution structures of the core light-harvesting a and b polypeptides from Rhodospirillum rubrum: Implications for the pigment-protein and protein-protein interactions. (2005)
  • Author(s): Wang, Z. -Y., Gokan., Kobayashi, M., Nozawa, T.
  • Journal Title: J. Mol. Biol. 347 Pages: 465-477
  • Peer Reviewed
• [Journal Article] Isotopic labeling of proteins by utilizing photosynthetic bacteria. (2005)
  • Author(s): Suzuki, H., Shimada, Y., Kobayashi, M., Kudo, M., Nozawa, T., Wang, Z. -Y.
  • Journal Title: Anal. Biochem. 347 Pages: 324-326
  • Peer Reviewed
• [Journal Article] Electronic properties and thermal stability of soluble redox proteins from a thermophilic purple sulfur photosynthetic bacterium, Thermochromatium tepidum. (2005)
  • Author(s): Kobayashi, M., Saito, T., Takahashi, K., Wang, Z. -Y., Nozawa, T.
  • Journal Title: Bull. Chem. Soc. Jpn. 78 Pages: 2164-2170
  • NAID: 10017074556
  • Peer Reviewed
• [Journal Article] Reconstitution of photosynthetic reaction centers and core antenna-reaction center complexes in liposomes and their thermal stability. (2005)
  • Author(s): Kobayashi, M., Fujioka, Y., Mori, T., Terashima, M., Suzuki, H., Shimada, Y., Saito, T., Wang, Z. -Y., Nozawa, T.
  • Journal Title: Biosci. Biotechnol. Biovhem. 69 Pages: 1130-1136
  • NAID: 130000030298
  • Peer Reviewed
• [Journal Article] Solution structures of the core light-harvesting α and β polypeptides from Rhodospirill um rubrum : Implications for the pigment-protein and protein-protein interactions (2005)
  • Author(s): Z.-Y.Wang, et al.
  • Journal Title: J.Mol.Biol. 347 Pages: 465-477
• [Journal Article] Reconstitution of photosynthetic reaction centers and core antenna-reaction center complexes in liposomes and their thermal stability (2005)
  • Author(s): Kobayashi, et al.
  • Journal Title: Biosci.Biotechnol.Biochem. 69 Pages: 1130-1136
  • NAID: 130000030298
• [Journal Article] Electronic properties and thermal stability of soluble redox proteins from a thermophilic sulfur photosynthetic bacterium, Thermochromatium tepidum (2005)
  • Author(s): Kobayashi, et al.
  • Journal Title: Bull.Chem.Soc.Jpn. 78 Pages: 2164-2170
• [Journal Article] Isotopic labeling of proteins by utilizing photosynthetic bacteria (2005)
  • Author(s): H.Suzuki, et al.
  • Journal Title: Anal.Biochem. 347 Pages: 324-326
• [Journal Article] Functional Expression and Characterization of a Bacterial Light-harvesting Membrane Proteins in E.coli and Cell-free Synthesis (2004)
  • Author(s): Yuichiro Shimada et al.
  • Journal Title: Bioscience Biotechnolgy and Biochemistry 68(9) Pages: 1942-1948
• [Journal Article] Reconstitution and replacement of bacteriochlorophyll a molecules in photosynthetic reaction center (2004)
  • Author(s): Masayuki Kobayashi et al.
  • Journal Title: J.Biochemistry 136 Pages: 363-369
  • NAID: 10016202243
• [Presentation] 好熱性光合成細菌由来の光捕集光電変換タンパク質の単離精製と結晶化 (2009)
  • Author(s): 小林美穂、清水佑記、堀口健太郎、大畠史一、干龍江、平野優、大友征宇
  • Organizer: 茨城地区交流会
  • Place of Presentation: 日立
  • Year and Date: 2009-11-06
• [Presentation] Crystal structure of a high-potential iron sulfur protein(HiPIP)at 0.5Å resolution (2009)
  • Author(s): K.Takeda, et.al
  • Organizer: 生物物理学会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] 緑色光合成細菌 Chlorobium tepidum 由来反応中心構成サブユニット cytochrome cの電子運搬ドメインの結晶構造 (2009)
  • Author(s): 平野優, et.al
  • Organizer: 生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-21
• [Presentation] 緑色光合成細菌由来のチトクロムczの構造的 (2009)
  • Author(s): 大友征宇、平野優、樋口誠, 木村行宏、大岡宏造, 三木邦夫
  • Organizer: 分光学的特徴について光合成セミナー
  • Place of Presentation: 京大
  • Year and Date: 2009-07-11
• [Presentation] 好熱性光合成細菌由来の Flavocytochrome cと Cytochrome c'の結晶構造解析と機能評価 (2009)
  • Author(s): 源氏梨恵, et.al
  • Organizer: 蛋白質学会
  • Place of Presentation: 熊本市
  • Year and Date: 2009-05-22
• [Presentation] 好熱性光合成細菌由来のFlavocytochrome oとCytochrome c'の結晶構造解析と機能評価 (2009)
  • Author(s): 源氏梨恵, 平野優, 木村行宏, 木村綾乃, 三木邦夫, 大友征宇
  • Organizer: 蛋白質学会
  • Place of Presentation: 熊本
• [Presentation] 超高エネルギーX線を利用した高電位鉄イオウタンパク質HiPIPの超高分解能結晶構造解析 (2009)
  • Author(s): 平野優, 竹田一旗, 蜘正室, 三木邦夫
  • Organizer: 蛋白質学会
  • Place of Presentation: 熊本
• [Presentation] 好熱性光合成細菌Thermochromatium trpodum由来cytochromeタンパク質flavocytochrome c552, cytochrome c'の結晶構造 (2008)
  • Author(s): 平野優, 木村行宏, 高崎将充, 牛島杏子, 木村綾乃, 源氏梨恵, 鈴木秀明, 三木邦夫, 大友征宇
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 好熱性光合成細菌Themochromatium tepidum由来cytochromeタンパク質flavocytochrome c552, cytochrome c'の結晶構造 (2008)
  • Author(s): 平野優, et.al.
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 好熱性光合成細菌乃Thermochromatium tepidum由来光捕集反応中心複合体におけるカルシウムイオンの構造的、機能的役割 (2008)
  • Author(s): Y. Kimura, L-J. Yu, YHirano, H. Suzuki, YKawamura, T. Ohno, J. -P Zhang, S. Otomo
  • Organizer: 日本生物物理学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-03
• [Presentation] 好熱性光合成細菌Themockromtium tepidum由来光捕集反応中心複合体におけるカルシウムイオンの構造的、機能的役割 (2008)
  • Author(s): 木村行宏, et.al.
  • Organizer: 日本生物物理学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-03
• [Presentation] 好熱性緑色光合成細菌由来Cytochrome czの大量発現とNMRによる構造機能評価 (2008)
  • Author(s): 樋口誠、逸見光、平野優、大岡宏造、大友征宇
  • Organizer: 第47回NMR討論会
  • Place of Presentation: 筑波大
  • Year and Date: 2008-11-12
• [Presentation] 好熱性緑色光合成細菌由来Cytochrome czの大量発現とNMRによる構造機能評価 (2008)
  • Author(s): 樋口誠, et.al.
  • Organizer: 第47回NMR討論会
  • Place of Presentation: 筑波大学
  • Year and Date: 2008-11-12
• [Presentation] Structural studies of the cytochrome c, from the green photosynthetic bacterium Chlorobium tepidum (2008)
  • Author(s): Y. Hirano, et.al.
  • Organizer: XXI Congress of the International Union of Crysta llography
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-08-23
• [Presentation] Sturctural and functional characterization of LH1 and core complexes from purple photosynthetic bacteria (2007)
  • Author(s): 大友征宇
  • Organizer: A joint seminar of UK-Japan Cooperation, Strategic International Cooperative Program (JST)
  • Place of Presentation: 関西学院大学
  • Year and Date: 2007-06-02
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• [Remarks] 茨城大学研究者情報総覧
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• [Remarks] 茨城大学研究者情報総覧
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