High resolution X-ray crystal structural analysis of biological macromolecular assemblies

• Project/Area Number: 16087206
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: TSUKIHARA Tomitake Osaka University, 蛋白質研究所, 特任研究員 (00032277)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Eiki 大阪大学, 蛋白質研究所, 助教 (00294132) ; TANAKA Hideaki 大阪大学, 蛋白質研究所, 助教 (40346169)
• Project Period (FY): 2004 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥196,300,000 (Direct Cost: ¥196,300,000); Fiscal Year 2009: ¥21,300,000 (Direct Cost: ¥21,300,000); Fiscal Year 2008: ¥15,700,000 (Direct Cost: ¥15,700,000); Fiscal Year 2007: ¥51,600,000 (Direct Cost: ¥51,600,000); Fiscal Year 2006: ¥53,800,000 (Direct Cost: ¥53,800,000); Fiscal Year 2005: ¥25,300,000 (Direct Cost: ¥25,300,000); Fiscal Year 2004: ¥28,600,000 (Direct Cost: ¥28,600,000)
• Keywords: 生体超分子 / 構造 / 機能 / X線結晶構造解析 / X 線結晶構造解析

Research Abstract

The goal of this research was to establish strategies by which we overcame limitation of molecular size and resolution in crystal structure analysis of biological macromolecular assemblies. We had two research plans. The first was to locate hydrogen atoms of cytochrome c oxidase with a molecular weight of 420 KDa at 1.6 A resolution. The second was to determine the structure of vault with a molecular weight of 10 MDa.Improving diffraction experiment method at BL44XU of SPring-8, we have successfully collected diffraction data of cytochrome c oxidase at 1.6 A resolution. We confirmed that the data set contained information of hydrogen atom positions. Structures of connexin26 gap junction channel and Exportin-5:RanGTP:pre-miRNA complex were determined by using intensity data sets acquired at the BL44XU.The structure of vault was determined by applying a newly developed method.

Research Products

• [Journal Article] A high resolution structure of the pre-microRNA nuclear export machinery. (2009)
  • Author(s): Chimari Okada, Eiki Yamashita, Soo Jae Lee, Satoshi Shibata, Jun Katahira, Atsushi Nakagawa, Yoshihiro Yoneda, Tomitake Tsukihara
  • Journal Title: Science 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] A description of the structural determination procedures of a gap junction channel at 3. 5 A resolution. (2009)
  • Author(s): Suga M, Maeda S, Nakagawa S, Yamashita E, Tsukihara T.
  • Journal Title: Acta Crystallogr. D65 Pages: 758-766
  • Peer Reviewed
• [Journal Article] Yoshinori Fujiyoshi and Tomitake Tsukihara, Structure of the connexin-26 gap junction channel at 3. 5 A resolution (2009)
  • Author(s): Shoji Maeda, So Nakagawa, Michihiro Suga, Eiki Yamashita, Atsunori Oshima
  • Journal Title: Nature 458 Pages: 597-602
  • Peer Reviewed
• [Journal Article] Substrate binding induces structural changes in cytochrome p450cam. (2009)
  • Author(s): K. Sakurai, H. Shimada, T. Hayashi, T. Tsukihara
  • Journal Title: Acta Cryst. F 65 Pages: 80-83
  • Peer Reviewed
• [Journal Article] A peroxide bridge between Fe and Cu ions in the O2 reduction site of fully oxidized cytochrome c oxidase could suppress the proton pump. (2009)
  • Author(s): H. Aoyama, K. Muramoto, K. Shinzawa-Itoh, K. Hirata, E. Yamashita, T. Tsukihara, T. Ogura, S. Yoshikawa
  • Journal Title: Proc. Natl. Acad. Sci. USA. 106 Pages: 2165-2169
  • Peer Reviewed
• [Journal Article] The structure of rat liver vault at 3. 5 angstrom resolution. (2009)
  • Author(s): H. Tanaka, K. Kato, E. Yamashita, T. Sumizawa, Y. Zhou, M. Yao, K. Iwasaki, M. Yoshimura, T. Tsukihara
  • Journal Title: Science 323 Pages: 384-388
  • Peer Reviewed
• [Journal Article] A high resolution structure of the pre-microRNA nuclear export machinery. (2009)
  • Author(s): Chimari Okada
  • Journal Title: Science 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] A description of the structural determination procedures of a gap junction channel at 3.5 A resolution. (2009)
  • Author(s): Michihiro Suga
  • Journal Title: Acta Crystallogr D65 Pages: 758-766
  • Peer Reviewed
• [Journal Article] Structure of the connexin-26 gap junction channel at 3.5 A resolution. (2009)
  • Author(s): Shoji Maeda
  • Journal Title: Nature 458 Pages: 597-602
  • Peer Reviewed
• [Journal Article] Substrate binding induces structural changes in cytochrome p450cam. (2009)
  • Author(s): Keisuke Sakurai
  • Journal Title: Acta Cryst.F 65 Pages: 80-83
  • Peer Reviewed
• [Journal Article] A peroxide bridge between Fe and Cu ions in the O_2 reduction site of fully oxidized cytochrome c oxidase suppresses the proton pump. (2009)
  • Author(s): Hiroshi Aoyama
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 2165-2169
  • Peer Reviewed
• [Journal Article] The structure of rat liver vault at 3.5 A resolution (2009)
  • Author(s): Hideaki Tanaka
  • Journal Title: Science 323 Pages: 384-388
  • Peer Reviewed
• [Journal Article] Structure of the connexin-26 gap junction channel at 3.5 A resolution (2009)
  • Author(s): S. Maeda
  • Journal Title: Nature (in press)
  • Peer Reviewed
• [Journal Article] Substrate binding induces structural changes in cytochrome p450cam (2009)
  • Author(s): K. Sakurai
  • Journal Title: Acta Cryst. F 65 Pages: 80-83
  • Peer Reviewed
• [Journal Article] A peroxide bridge between Fe and Cu ions in the O2 reduction site of fully oxidized cytochrome c oxidase could suppress the proton punp (2009)
  • Author(s): H. Aoyama
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 2165-2169
  • Peer Reviewed
• [Journal Article] The structure of rat liver vault at 3.5 angstrom resolution (2009)
  • Author(s): H. Tanaka
  • Journal Title: Science 323 Pages: 384-388
  • Peer Reviewed
• [Journal Article] Structural analysis of Arabidopsis CnfU protein: an iron-sulfur cluster biosynthetic scafold in chloroplasts. (2008)
  • Author(s): T. Yabe, E. Yamashita, A. Kikuchi, K. Morimoto, A. Nakagawa, T. Tsukihara, M. Nakai
  • Journal Title: J. Mol. Biol. 381 Pages: 160-173
  • Peer Reviewed
• [Journal Article] A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry. (2008)
  • Author(s): Koji Kato, Hideaki Tanaka, Tomoyuki Sumizawa, Masato Yoshimura, Eiki Yamashita, Kenji Iwasaki, Tomitake Tsukihara.
  • Journal Title: Acta Crystallogr. D64 Pages: 525-531
  • Peer Reviewed
• [Journal Article] Structure of human monoamine oxidase at 2. 2 A resolution: The control of opening the entry for substrates/inhibitors. (2008)
  • Author(s): Se-Young Son, Jichun Ma, Youhei Kondou, Masato Yoshimura, Eiki Yamashita,, Tomitake Tsukihara.
  • Journal Title: Proc. Natl. Acad. USA 105 Pages: 5739-5744
  • Peer Reviewed
• [Journal Article] Structural analysis of Arabidopsis CnfU protein : an iron-sulfur cluster biosynthetic scafold in chloroplasts (2008)
  • Author(s): T. Yabe
  • Journal Title: J. Mol. Biol 381 Pages: 160-173
  • Peer Reviewed
• [Journal Article] A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry (2008)
  • Author(s): Koji Kato
  • Journal Title: Acta Crystallogr. D 64 Pages: 525-531
  • Peer Reviewed
• [Journal Article] Structure of human monoamine oxidase at 2.2 A resolution : The control of opening the entry for substrates/inhibitors (2008)
  • Author(s): Se-Young Son
  • Journal Title: Proc. Natl. Acad. USA 105 Pages: 5739-5744
  • Peer Reviewed
• [Journal Article] A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry. (2008)
  • Author(s): Kato, K.
  • Journal Title: Acta Cryst. D (In press)
  • Peer Reviewed
• [Journal Article] A histidine residue as a controlling site for dioxygen reduction and proton pumping of cytochrome c oxidase (2007)
  • Author(s): Kazumasa Muramoto, Kunio Hirata, Kyoko Shinzawa-Itoh, Shinji Yoko-o, Eiki Yamashita, Hiroshi Aoyama, Tomitake Tsukihara,, Shinya Yoshikawa.
  • Journal Title: Proc. Natl. Acad. USA 104 Pages: 7881-7886
  • Peer Reviewed
• [Journal Article] The crystal structure of a virus-like particle from the hyperthermophilic archaeon Pyrococcus furiosus provides insight into the evolution of viruses. (2007)
  • Author(s): Fusamichi Akita, Khoon Tee Chong, Hideaki Tanaka, Eiki Yamasita, Naoyuki Miyazaki, Yuichiro Nakaishi, Mamoru Suzuki, Kazuhiro Namba, Yasuko Ono, Tomitake Tsukihara,, Atsushi Nakagawa
  • Journal Title: J. Mol. Biol. 368 Pages: 1469-1483
  • Peer Reviewed
• [Journal Article] Structures and physiological roles of all the integral lipids of bovine heart cytochrome c oxidase. (2007)
  • Author(s): Kyoko Shinzawa-Itoh, Hiroshi Aoyama, Kazumasa Muramoto, Hirohito Terada, Tsuyoshi Kurauchi, Yoshiki Tadehara, Akiko Yamasaki, Takashi Sugimura, Sadamu Kurono, Kazuo Tsujimoto, Tsunehiro Mizushima, Eiki Yamashita, Tomitake Tsukihara, Shinya Yoshikawa
  • Journal Title: EMBO J. 26 Pages: 1713-1725
  • Peer Reviewed
• [Journal Article] The Proton-Pumping Pathway of Bovine Heart Cytochrome c Oxidase. (2007)
  • Author(s): Kunitoshi Shimokata, Yukie Katayama, Haruka Murayama, Makoto Suematsu, Tomitake Tsukihara, Kazumasa Muramoto, Shinya Yoshikawa,, Hideo Shimada
  • Journal Title: Proc. Natl. Acad. USA 104 Pages: 4200-4205
  • Peer Reviewed
• [Journal Article] A histidine residue as a controlling site for dioxygen reduction and protonpumpping of cytochrome c oxidase. (2007)
  • Author(s): Muramoto, K.
  • Journal Title: Proc. Natl. Acad. USA 104 Pages: 7881-7886
  • Peer Reviewed
• [Journal Article] The crystal structure of a virus-like particle from the hyperthermophilic archaeon Pyrococcus furiosus provides insight into the evolution of viruses. (2007)
  • Author(s): Akita, F.
  • Journal Title: J, Mol. Biol. 368 Pages: 1469-1483
  • Peer Reviewed
• [Journal Article] Structures and physiological reles of all the integral lipids of bovine heart cytochrome c oxidase. (2007)
  • Author(s): Shinzawa-Itoh, K.
  • Journal Title: EMBO J. 26 Pages: 1713-1725
  • Peer Reviewed
• [Journal Article] The Proton-Pumping Pathway of Bovine Heart Cytochrome c Oxidase. (2007)
  • Author(s): Shimokata, T.
  • Journal Title: Proc. Natl. Acad. USA 104 Pages: 4200-4205
  • Peer Reviewed
• [Journal Article] The proton pumping pathway of bovine heart cytochrome c oxidase. (2007)
  • Author(s): K.Shimokata
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 4200-4205
• [Journal Article] Structures and physiological roles of all the integral lipids of bovine heart cytochrome c oxidase. (2007)
  • Author(s): K.Shinzawa-Itoh
  • Journal Title: EMBO J. (in press)
• [Journal Article] A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase. (2007)
  • Author(s): K.Muramoto
  • Journal Title: Proc. Natl. Acad. Sci. USA (in press)
• [Journal Article] Structure of coenzyme A-disulfide reductase from Staphylococcus aureus at 1. 54 A resolution. (2006)
  • Author(s): Mallett TC, Wallen JR, Karplus PA, Sakai H, Tsukihara T, Claiborne A.
  • Journal Title: Biochemistry. 45 Pages: 11278-11289
  • Peer Reviewed
• [Journal Article] The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold. (2006)
  • Author(s): Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M.
  • Journal Title: J Mol Biol. 360 Pages: 117-132
  • Peer Reviewed
• [Journal Article] The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold. (2006)
  • Author(s): K.Morimoto
  • Journal Title: J Mol Biol. 360 Pages: 117-132
• [Journal Article] Structure of coenzyme A-disulfide reductase from Staphylococcus aureus at 1.54 A resolution. (2006)
  • Author(s): T.C.Mallet
  • Journal Title: Biochemistry 45 Pages: 11278-11289
• [Journal Article] Preliminary crystallographic analysis of the antibiotic discharge outer membrane lipoprotein OprM of Pseudomonas aeruginosa with an exceptionally long unit cell and complex lattice structure. (2005)
  • Author(s): Akama H, Kanemaki M, Tsukihara T, Nakagawa A, Nakae T
  • Journal Title: Acta Crystallogr. F61 Pages: 131-133
  • Peer Reviewed
• [Journal Article] Conformational Changes in the Tryptophan Synthase from a Hyperthermophile. (2005)
  • Author(s): S.J. Lee, K. Ogasahara, J. Ma, K. Nishio, M. Ishida, Y. Yamagata, T. Tsukihara,, K. Yutani.
  • Journal Title: Biochemistry 44 Pages: 11417-11427
  • Peer Reviewed
• [Journal Article] Absolute configuration of the hydroxyfarnesylethyl group of haem A, determined by X-ray structural analysis of bovine heart cytochrome c oxidase using methods applicable at 2. 8 A resolution. (2005)
  • Author(s): E. Yamashita, H. Aoyama, M. Yao, K. Muramoto, K. Shinzawa-Itoh, S. Yoshikawa,, T. Tsukihara
  • Journal Title: Acta Cryst. D61 Pages: 1373-1377
  • Peer Reviewed
• [Journal Article] Ab initio crystal structure determination of spherical viruses that exhibit a centrosymmetric location in the unit cell. (2005)
  • Author(s): J. Taka, H. Naitow, M. Yoshimura, N. Miyazaki, A. Nakagawa,, T. Tsukihara.
  • Journal Title: Acta Cryst. D61 Pages: 1099-1106
  • Peer Reviewed
• [Journal Article] Structure of the Central Hub of Bacteriophage Mu Baseplate Determined by X-ray Crystallography of gp44. (2005)
  • Author(s): Y. Kondou, D. Kitagawa, S. Takeda, Y. Tsuchiya, E. Yamashita, M. Mizuguchi, K. Kawano,, T. Tsukihara
  • Journal Title: J. Mol. Biol. 352 Pages: 976-985
  • Peer Reviewed
• [Journal Article] Preliminary Crystallographic analysis of the antibiotic discharge outer membrane lipoprotein OprM of Pseudomonas aeruginosa with an exceptionally long unit cell and complex lattice structure. (2005)
  • Author(s): H. Akama, M. Kanemaki, T. Tsukihara, A. Nakagawa, T. Nakae
  • Journal Title: Acta Cryst. F61 Pages: 131-133
  • Peer Reviewed
• [Journal Article] Conformational Change in theα-Subunit Coupled to Binding of theβ2-Subunit of Tryptophan Synthase from Escherichia coli: Crystal Structure of the Tryptophan Synthaseα-Subunit Alone. (2005)
  • Author(s): K. Nishio, Y. Morimoto, M. Ishizuka, K. Ogasawara, T. Tsukihara, K. Yutani
  • Journal Title: Biochemistry 44 Pages: 1184-1192
  • Peer Reviewed
• [Journal Article] Transcription and the conserved interactions of two major structural proteins of a pair of Phytoreoviruses confirm the mechanism of assembly of the outer capsid layer. (2005)
  • Author(s): Miyazaki, N., Hagiwara, K., Naitow, H., Higashi, T., Cheng, R.H., Tsukihara, T., Nakagawa, A., Omura, T.
  • Journal Title: J. Mol. Biol. 345 Pages: 229-237
  • Peer Reviewed
• [Journal Article] Ab initio crystal structure determination of spherical viruses that exhibit a centrosymmetric location in the unit cell. (2005)
  • Author(s): J.Taka
  • Journal Title: Acta Cryst. D61 Pages: 1099-1106
• [Journal Article] Absolute configuration of the hydroxyfarnesylethyl group of haem A, determined by X-ray structural analysis of bovine heart cytochrome c oxidase using methods applicable at 2.8A resolution. (2005)
  • Author(s): E.Yamashita
  • Journal Title: Acta Cryst. D61 Pages: 1373-1377
• [Journal Article] Structure of the Central Hub of Bacteriophage Mu Baseplate Determined by X-ray Crystallography of gp44. (2005)
  • Author(s): Y.Kondou
  • Journal Title: J.Mol.Biol. 352 Pages: 976-985
• [Journal Article] Preliminary Crystallographic analysis of the antibiotic discharge outer membrane lipoprotein OprM of Pseudomonas aeruginosa with an exceptionally long unit cell and complex lattice structure. (2005)
  • Author(s): H.Akama, et al.
  • Journal Title: Acta Crysta. F61 Pages: 131-133
• [Journal Article] Conformational Change in the α-Subunit Coupled to Binding of the β2-Subunit of Tryptopha Synthase from Escherichia coli: Crystal Structure of the Tryptophan Synthase α-Subunit Alone. (2005)
  • Author(s): K.Nishi, et al.
  • Journal Title: Biochemistry 44 Pages: 1184-1192
• [Journal Article] Transcription and the conserved interactions of two major structural proteins of a pair of Phytoreoviruses confirm the mechanism of assembly of the outer capsid layer (2005)
  • Author(s): Miyazaki.N, et al.
  • Journal Title: J.Mol.Biol. 345 Pages: 229-237
• [Presentation] 大きさと精密さに挑む生体超分子の結晶構造解析 (2010)
  • Author(s): 月原冨武
  • Organizer: 第23回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: イーグレひめじ、姫路
• [Presentation] 高精度の異常分散効果の測定によって明らかになった事実:酸化型チトクロム酸化酵素の還元中心Fe-Cu間にCl^-イオンは存在しない (2010)
  • Author(s): 菅倫寛
  • Organizer: 第23回日本放射光学会年会
  • Place of Presentation: イーグレ姫路,姫路
• [Presentation] S低・中分解能データから引き出される超分子の振るまい-高難度タンパク質複合体の構造解析を目指して (2009)
  • Author(s): 山下栄樹
  • Organizer: 日本結晶学会
  • Place of Presentation: 関西学院大学(兵庫県)
  • Year and Date: 2009-12-05
• [Presentation] 分子量約1000万の核酸-タンパク質複合体vaultのX線結晶構造解析 (2009)
  • Author(s): 田中秀明
  • Organizer: 日本結晶学会
  • Place of Presentation: 関西学院大学(兵庫県)
  • Year and Date: 2009-12-05
• [Presentation] 細胞間連絡を司るギャップ結合チャネルのX線結晶構造 (2009)
  • Author(s): 前田将司
  • Organizer: 大阪大学理学部生物科学研究科60周年記念シンポジウム
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2009-10-10
• [Presentation] Purification and structure/function of connexin 26 gap junction (2009)
  • Author(s): Shoji Maeda
  • Organizer: Osaka/Glasgow structural biology mini-symposium
  • Place of Presentation: Glasgow, Scotland
  • Year and Date: 2009-02-23
• [Presentation] The structure of rat liver vault (2009)
  • Author(s): Tomitake Tsukihara
  • Organizer: AsCA '09 Joint conference of the Asian crystallographic society and Chinese crystallography society
  • Place of Presentation: Jingyi Hotel, Beijing, China
• [Presentation] Structure of human gap junction channel (2009)
  • Author(s): Shoji Maeda
  • Organizer: International Gap Junction Conference 2009
  • Place of Presentation: Hilton Sedona Resort and Spa, Sedona, Arizona
• [Presentation] Structure and Function of Cytochrome c Oxidase, The 19th Symposium on Role of Metals in Biological Reactions (2009)
  • Author(s): 月原冨武
  • Organizer: Biology and Medidine (SRM2009)
  • Place of Presentation: Icho Kaikan, Osaka
• [Presentation] 巨大複合体結晶の放射光X線データ測定と処理-巨大超分子の構造に原子レベルで迫る (2009)
  • Author(s): 山下栄樹
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 全日空ホテルニュースカイ, 熊本
• [Presentation] x線による生体超分子構造研究のいま (2009)
  • Author(s): 月原冨武
  • Organizer: 第50回日本生化学会
  • Place of Presentation: とりぎん文化会館・鳥取県鳥取市
• [Presentation] X-ray crystal structure of human gap junction channel (2009)
  • Author(s): Shoji M
  • Organizer: NTHU-Osaka minisymposium
  • Place of Presentation: Hsinchu, Taiwan
• [Presentation] X線による生体超分子構造研究のいま (2009)
  • Author(s): 月原冨武
  • Organizer: 第50回日本生化学会
  • Place of Presentation: とりぎん文化会館・鳥取県鳥取市
• [Presentation] 巨大複合体結晶の放射光X線データ測定と処理-巨大超分子の構造に原子レベルで迫る (2009)
  • Author(s): 山下栄樹
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 全日空ホテル ニュースカイ,熊本
• [Presentation] ギャップ結合チャネルのX線結晶構造 (2009)
  • Author(s): 前田将司
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 全日空ホテル ニュースカイ,熊本
• [Presentation] ギャップジャンクションチャネルの構造決定手順 (2009)
  • Author(s): 菅倫寛
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 全日空ホテル ニュースカイ,熊本
• [Presentation] Structure and Function of Cytochrome c Oxidase (2009)
  • Author(s): 月原冨武
  • Organizer: The 19^<th> Symposium on Role of Metals in Biological Reactions, Biology and Medidine (SRM2009)
  • Place of Presentation: Icho Kaikan, Osaka University, Suita, Osaka, Japan
• [Presentation] Structural insight into heterotypic channel compatibility among connexins by homology modeling (2009)
  • Author(s): So Nakagawa
  • Organizer: International gap junction conference 2009
  • Place of Presentation: Hilton Sedona Resort and Spa, Sedona, Arizona
• [Presentation] Structure of human gap junction channel (2009)
  • Author(s): Shoji M
  • Organizer: International Gap Junction Conference 2009, 25-30 July, 2009, Sedona, USA
  • Place of Presentation: Hilton Sedona Resort and Spa, Sedona, Arizona
• [Presentation] Accurate measurements of anomalous dispersion effect shows there is no chloride ion between Fe and Cu ions in the reduction site of oxidized Cytochrome c Oxidase (2009)
  • Author(s): Michihiro Suga
  • Organizer: AsCA '09 Joint conference of the Asian crystallogr aphic society and Chinese crystallography society
  • Place of Presentation: Jingyi Hotel, Beijing, China
• [Presentation] The structure of human connexin 26 gap junction channel (2009)
  • Author(s): Shoji M
  • Organizer: AsCA '09 Joint conference of the Asian crystallographic society and Chinese crystallography society
  • Place of Presentation: Jingyi Hotel, Beijing, China
• [Presentation] Structural insight into heterotypic channel compatibility among connexins by homology modeling (2009)
  • Author(s): So Nakagawa
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島県徳島市・アスティ徳島
• [Presentation] ヒト由来コネキシン26ギャップ結合チャネルのX線結晶構造 (2009)
  • Author(s): 前田将司
  • Organizer: 日本生物物理学会第47会年会
  • Place of Presentation: 徳島県徳島市・アスティ徳島
• [Presentation] ラット肝臓由来ボルトの3.5A分解能での構造解析 (2009)
  • Author(s): 加藤公児
  • Organizer: 日本生物物理学会第47会年会
  • Place of Presentation: 徳島県徳島市・アスティ徳島
• [Presentation] Structure of human connexin 26 gap junction channel (2009)
  • Author(s): So Nakagawa
  • Organizer: Osaka University Macromolecular Symposium'09
  • Place of Presentation: 大阪大学豊中キャンパス
• [Presentation] X-ray structural analysis of bovine cytochrome c oxidase (2008)
  • Author(s): 月原冨武
  • Organizer: International Symposium on Membrane Proteins and High Resolution X-ray Structural Analysis
  • Place of Presentation: 兵庫県赤穂郡先端科学技術支援センター
  • Year and Date: 2008-09-01
• [Presentation] 呼吸酵素チトクロム酸化酵素の働きの仕組み (2008)
  • Author(s): 月原冨武
  • Organizer: 千里ライフサイエンスセミナー「生命機能を支える生体超分子の高次構造と機能」
  • Place of Presentation: 大阪府豊中市千里ライフサイエンスセンター
  • Year and Date: 2008-02-28
• [Presentation] ヒト由来コネキシン26ギャップ結合チャネルのX線結晶構造解析 (2008)
  • Author(s): 前田将司
  • Organizer: 第7回コネキシン研究会
  • Place of Presentation: 京都府京都市ホテルルビノ京都堀川
• [Presentation] X-ray structure of the vault purified from rat liver (2008)
  • Author(s): 田中秀明
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
• [Presentation] X-ray structure of Human gap Junction Channel (2008)
  • Author(s): 前田将司
  • Organizer: 第21回国際結晶学会年会
  • Place of Presentation: 大阪府大阪市グランキューブ大阪
• [Presentation] Evidence for the H-channel proton pump in bovine cytochrome c oxidase (2008)
  • Author(s): Shinya Yoshikawa
  • Organizer: 15th European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] ウイルスなどの生体超分子のx線結晶構造解析 (2008)
  • Author(s): 月原冨武
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 山梨県南都留郡ラフォーレ山中湖
• [Presentation] Structure of a large ribonucleoprotein complex (2008)
  • Author(s): 加藤公児
  • Organizer: 生体防御医学研究所第17回ホットスプリングハーバー国際シンポジウム/第3回研究所ネットワーク国際シンポジウム合同シンポジウム
  • Place of Presentation: 大分県別府市別府亀の井ホテル
• [Presentation] ラット肝臓由来vaultのX線結晶構造解析 (2008)
  • Author(s): 加藤公児
  • Organizer: 特定領域研究丁生体超分子の構造形成と機能制御の原子機構」第4回ワークショップ
  • Place of Presentation: 兵庫県淡路市淡路夢舞台国際会議場
• [Presentation] ヒト由来ギャップ結合チャネルのX線結晶構造解析 (2008)
  • Author(s): 前田将司
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第4回ワークショップ
  • Place of Presentation: 兵庫県淡路市淡路夢舞台国際会議場
• [Presentation] ウイルスなどの生体超分子のX線結晶構造解析 (2008)
  • Author(s): 月原冨武
  • Organizer: 第55何毒素シンポジウム
  • Place of Presentation: 山梨県南都留郡ラフォーレ山中湖
• [Presentation] X-ray crystal structural analysis of cyanide binding cytochrome coxidase (2008)
  • Author(s): Masao Mochizuki
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] A peroxide bridge between the two metals in the dinuclear center of the fully oxidized cytochrome coxidase (2008)
  • Author(s): Hiroshi Aoyama
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] Synthesis of functional Paracoccus Denitrificans cytochrome c oxidase by Escherichia coli cell-free coupled transcription/translation system (2008)
  • Author(s): Yukie Katayarna
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] Mutations of possible proton-transfer pathways of bovine heart cytochrome c oxidase (2008)
  • Author(s): Kunitoshi Shimokata
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] X-ray structure of carbon monoxide at copper site of the dinuclear site of cytochrome c oxidase (2008)
  • Author(s): Kazumasa Muramoto
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] Structural analyses for lipid/protein interactions in bovine heart cytochrome c oxidase (2008)
  • Author(s): Kyoko Shinzawa-Itoh
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] X-ray structural analysis of zinc/cadmium inhibitory site in bovine heart cytochrome c oxidase (2008)
  • Author(s): Kazumasa Muramoto
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] Evidence for the H-channel proton pump in bovine cytochrome c oxidase (2008)
  • Author(s): Shinya Yoshikawa
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin, Ireland
• [Presentation] Structure of vault purified from rat liver (2008)
  • Author(s): 加藤公児
  • Organizer: 第21回国際結晶学会年会
  • Place of Presentation: 大阪府大阪市グランキューブ大阪
• [Presentation] Crystal structure of human gap junction channel (2008)
  • Author(s): 前田将司
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
• [Presentation] Refined structural analysis of carbon monoxide and nitric oxide at the dinuclear site of cytochrome c oxidase (2008)
  • Author(s): 村本和優
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
• [Presentation] X-ray structural analysis of zinc/cadmium inhibitory site in bovine heart cyiochrome c oxidase (2008)
  • Author(s): 前由和範
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
• [Presentation] High resolution and precise X-ray crystal structure determiantion of Cyctochrome c Oxidase (2008)
  • Author(s): 菅倫寛
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
• [Presentation] ヒト由来コネキシン26ギャップ結合チャネルのX線結晶構造解析 (2008)
  • Author(s): 前田将司
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
  • Place of Presentation: 茨城県つくば市つくば国際会議場
• [Presentation] ラット肝臓由来vaultのX線結晶構造解析 (2008)
  • Author(s): 加藤公児
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
  • Place of Presentation: 茨城県つくば市つくば国際会議場
• [Presentation] Probing a Molecular Vibration of Cytochrome c Oxidase during Tumover in Whole Mitochondria (2007)
  • Author(s): 小倉尚志
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
• [Presentation] ウシチトクロム酸化酵素の水形成用プロトン輸送経路D-pathwayの変異体解析 (2007)
  • Author(s): 下方国稔
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
• [Presentation] ラット肝臓由来vaultのX線結晶構造解析 (2007)
  • Author(s): 田中秀明
  • Organizer: 日本結晶学会2007年度年会
  • Place of Presentation: 東京工業大岡山キャンパス
• [Presentation] Rotational symmetry of vault from rat liver (2007)
  • Author(s): Tanaka, H.
  • Organizer: ISDSB2007
  • Place of Presentation: 東京都江戸川区タワーホール船堀
• [Presentation] 大腸菌無細胞転写翻訳系によるシトクロム酸化酵素の合成 (2007)
  • Author(s): 片山幸江
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター
• [Presentation] ラット肝臓由来vaultのX線結晶構造解析 (2007)
  • Author(s): 田中秀明
  • Organizer: 日本結晶学会2007年度年会
  • Place of Presentation: 東京工業大学大岡山キャンパス
• [Presentation] Probing a Molecular Vibration of Cytochrome c Oxidase during Tum over in Whole Mitochondria (2007)
  • Author(s): 小倉 尚志
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] Paracoccus denitirficans チトクロム酸化酵素遺伝子の大腸菌無細胞転写翻沢系での発現に及ぼすYidC強制発現の効果 (2007)
  • Author(s): 片山 幸江
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] 酸化型ウシ心筋チトクロム酸化酵素のX線構造 (2007)
  • Author(s): 青山 浩
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] ウシチトクロム酸化酵素の水形成用プロトン輸送経路D-Pathwayの変異体解析 (2007)
  • Author(s): 下方 国稔
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] ウシ心筋チトクロム酸化酵素のzn阻害部位のX線構造解析 (2007)
  • Author(s): 村本 和優
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] 部分還元型ウシ心筋チトクロム酸化酵素のx線構造解析 (2007)
  • Author(s): 谷口 槙
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] シアン化物結合型チトクロム酸化酵素のX線結晶構造解析 (2007)
  • Author(s): 望月 正雄
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 神奈川県横浜市 パイフィコ横浜
• [Presentation] 結晶構造解析が切り開くバイオ・医療科学技術「構造生物学の近未来と放射光結晶学」 (2006)
  • Author(s): 月原冨武
  • Organizer: 結晶を制するものは世界を制す～材料からの日本活性化～(主催:日本結晶成長学会)
  • Place of Presentation: 学術総合センター・一橋記念講堂
  • Year and Date: 2006-04-07
• [Presentation] ウシ心筋チトクロム酸化酵素のヘムAのファーネシルエチル基の絶対構造 (2005)
  • Author(s): 青山浩
  • Organizer: 日本結晶学会2005年度年会
  • Place of Presentation: イーグレひめじ
• [Presentation] 高分解能構造解析によるチトクロム酸化酵素のプロトンポンプ機構 (2005)
  • Author(s): 月原冨武
  • Organizer: 第43回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 緑膿菌に発現する薬剤排出ポンプのX線結晶構造解析 (2005)
  • Author(s): 赤間浩之
  • Organizer: 第5回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 結晶構造解析に基づいた化学反応機構研究の概要 (2004)
  • Author(s): 月原冨武
  • Organizer: 第42回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] ラット肝臓由来vaultのX線結晶構造解析 (2004)
  • Author(s): 田中秀明
  • Organizer: 日本結晶学会2004年度年会及び総会
  • Place of Presentation: 大阪大学
• [Presentation] Crystal structure of OprM, a xenobiotic-ejecting outer membrane component, revealed two modes of membrane anchoring and tightly occluded cavity end (2004)
  • Author(s): Taiji Nakae
  • Organizer: 第77回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Book] Protein Crystallarography in Drug Discovery(R.E. Babine and S.S. Abdel-Meguid) (2004)
  • Author(s): T. Mizushima, et, al.
  • Total Pages: 278
• [Book] Protein Crystallarography in Drug Discovery (2004)
  • Author(s): T.Mizushima, et al.
  • Total Pages: 278
  • Publisher: R.E.Babine and S.S.Abdel-Meguid
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/olabb/tsukihara/
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/olabb/tsukihara/houdou/
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/crystallography/tokutei/index.htm
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/crystallography/tokutei/index.htm
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/crystallography/tokutei/index.htm