| Budget Amount *help |
¥202,400,000 (Direct Cost: ¥202,400,000)
Fiscal Year 2009: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2008: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2007: ¥20,000,000 (Direct Cost: ¥20,000,000)
Fiscal Year 2006: ¥20,000,000 (Direct Cost: ¥20,000,000)
Fiscal Year 2005: ¥103,200,000 (Direct Cost: ¥103,200,000)
Fiscal Year 2004: ¥35,200,000 (Direct Cost: ¥35,200,000)
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| Research Abstract |
To elucidate the mechanisms of large biological macromolecular complexes underlying various biological functions and to make use of them in the application for medicine and nanotechnology, it is essential to visualize their 3D structures in atomic detail. Electron cryomicroscopy (cryoEM) and image analysis of large complexes, although difficult to achieve atomic resolution by itself, can be combined with X-ray crystallography of component molecules to build pseudo-atomic models of the complexes. We have improved the resolution and throughput of cryoEM image analysis to increase the accuracy and efficiency of atomic model building of the complexes markedly to facilitate our understanding of the biological mechanisms.
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