The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.

• Project/Area Number: 16087208
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Hyogo
• Principal Investigator: YOSHIKAWA Shinya University of Hyogo, 大学院・生命理学研究科, 教授 (40068119)
• Co-Investigator(Kenkyū-buntansha): MURAMOTO Kazumasa 兵庫県立大学, 大学院・生命理学研究科, 准教授 (50305679) ; ITOH Kyoko 兵庫県立大学, 大学院・生命理学研究科, 助教 (70206316)
• Project Period (FY): 2004 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥85,100,000 (Direct Cost: ¥85,100,000); Fiscal Year 2009: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2008: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2007: ¥16,500,000 (Direct Cost: ¥16,500,000); Fiscal Year 2006: ¥16,500,000 (Direct Cost: ¥16,500,000); Fiscal Year 2005: ¥17,400,000 (Direct Cost: ¥17,400,000); Fiscal Year 2004: ¥8,700,000 (Direct Cost: ¥8,700,000)
• Keywords: 酸素反応機構 / 膜蛋白質 / ミトコンドリア / エネルギー変換 / X線結晶構造解 / チトクロム酸化酵素 / NADH-ユビキノン還元酵素 / X線結晶構造解析 / 酵素反応機構 / 複合体I / X線構造解析 / 膜タンパク質 / 結晶化 / 界面活性剤

Research Abstract

Complete structures of the intrinsic lipids in bovine cytochrome c oxidase were determined. His503 couples the proton-pumping process with O_2 reduction process. Resting oxidized enzyme has a peroxide bridged in the O_2 reduction site. The bound O_2^- at Fe_<a3>^<2+> is completely reduced by a non-consecutive three electron donation. The water-channel of the H-channel blocks the reverse proton leak to provide the highly effective energy coupling. Two dimensional crystals of NADH-ubiquinone reductase and F0F1ATPase were obtained. Highly sensitive infrared apparatus applicable for any protein system in aqueous solution was constructed.

Research Products

• [Journal Article] Bovine cytochrome c oxidase otructures enable O2 reduction with minimization of active oxygen species and provide a proton pumping gate (2010)
  • Author(s): K. Muramoto, K. Ohta, K. Shinzawa-Itoh, K. Kanda, M. Taniguchi, E. Yamashita, T. Tsukihara, S. Yoshikawa
  • Journal Title: Proc. Natl. Acad. Sci. USA (in press)
  • Peer Reviewed
• [Journal Article] A Resonance Raman Band Assignable to the O7O Stretching Mode in the Resting Oxidized State of Bovine Heart Cytochrome c Oxidase (2010)
  • Author(s): M. Sakaguchi, K. Shinzawa-Itoh, S. Yoshikawa, T. Ogura
  • Journal Title: J. Bioenergetics and Biomembranes (in press)
  • Peer Reviewed
• [Journal Article] Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein (2010)
  • Author(s): Y. Katayama, K. Shimokata, M. Suematsu, T. Ogura, T. Tsukihara, S. Yoshikawa, H. Shimada
  • Journal Title: J. Bioenergetics and Biomembranes (in press)
  • Peer Reviewed
• [Journal Article] X-ray structure of the NO-bound Cu(B) in bovine cytochrome c oxidase. (2010)
  • Author(s): Ohta K, Muramoto K, Shinzawa-Itoh K, Yamashita E, Yoshikawa S, Tsukihara T
  • Journal Title: Acta Cryst. F 66 Pages: 251-253
  • Peer Reviewed
• [Journal Article] Bovine heart NADH-ubiquinone oxidoreductae contains one molecule of ubiquinone with ten isoprenoide units as one of the cofactors. (2010)
  • Author(s): K. Shinzawa-Itoh, J. Seiyama, H. Terada, R. Nakatsubo, K. Naoki, Y. Nakashima, S. Yoshikawa
  • Journal Title: Biochemistry 49 Pages: 487-492
  • Peer Reviewed
• [Journal Article] Bovine heart NADH-ubiquinone oxidoreductae contains one molecule of ubiquinone with ten isoprenoide units as one of the cofactors. (2010)
  • Author(s): Kvoko Shinzawa-Itoh
  • Journal Title: Biochemistry 49 Pages: 487-492
  • Peer Reviewed
• [Journal Article] X-ray structure of the NO-bound Cu(B) in bovine cytochrome coxidase. (2010)
  • Author(s): Ohta K
  • Journal Title: Acta Cryst.F 66 Pages: 251-253
  • Peer Reviewed
• [Journal Article] Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein (2010)
  • Author(s): Yukie Katayama
  • Journal Title: J.Bioenergetics and Biomembranes (in press)
  • Peer Reviewed
• [Journal Article] AResonance Raman Band Assignable to the O-O Stretching Modein the Resting Oxidized State of Bovine Heart Cytochrome c Oxidase (2010)
  • Author(s): Miyuki Sakaguchi
  • Journal Title: J.Bioenergetics and Biomembranes (in press)
  • Peer Reviewed
• [Journal Article] Bovine cytochrome c oxidase otructures enabie O_2 reduction with minimization of active oxygen species and provide a proton pumping gate (2010)
  • Author(s): Kazumasa Muramoto
  • Journal Title: Proc.Natl.Acad.Sci.USA (in press)
  • Peer Reviewed
• [Journal Article] A Peroxide Bridge between Fe and Cu Ions in the O2 Reduction Site of Fully Oxidized Cytochrome c Oxidase could suppress the Proton Pump (2009)
  • Author(s): H. Aoyama, K. Muramoto, K. Shinzawa-Itoh, K. Hirata, E. Yamashita, T. Tsukihara, T. Ogura, S. Yoshikawa
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 2165-2169
  • Peer Reviewed
• [Journal Article] 脂質-蛋白質相互作用の解析-ウシ心筋シトクロム酸化酵素を中心として- (2009)
  • Author(s): 吉州信也
  • Journal Title: 蛋白質 核酸 酵素 54 Pages: 1827-1836
  • Peer Reviewed
• [Journal Article] A Peroxide Bridge between Fe and Cu Ions in the O_2 Reduction Site of Fully Oxidized Cvtochrome c Oxidase could sunnress the Proton Pumn (2009)
  • Author(s): Hiroshi Aoyama
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 2165-2169
  • Peer Reviewed
• [Journal Article] Iron-sulfur cluster N5 is coordinated by a HXXXCXXCXXXXXC motife in the NuoG subunit of E. coli NADH:quinine oxidoreductase (Complex I) (2008)
  • Author(s): Nakamura-Ogiso, E., Matsuno-Yagi, A., Yoshikawa, S., Yagi, T., Ohnishi, T.
  • Journal Title: J. Biol. Chem. 283 Pages: 25979-25987
  • Peer Reviewed
• [Journal Article] Functional role of Coenzyme Q in the energy coupling of NADH-CoQ oxidoreductase (Complex I): Stabilization of the semiquinone state with application of inside-positive membrane potential to proteoliposomes (2008)
  • Author(s): Ohnishi, To., Ohnishi, Ts., Shinzawa-Itoh, K., Yoshikawa, S.
  • Journal Title: Biofctor 32 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Red-Excitation Resonance Raman Analysis of the nFe=O Mode of Ferryl-Oxo Hemoproteins (2008)
  • Author(s): Ikemura, K., Mukai, M., Shimada, H., Tsukihara, T., Yamaguchi, S., Shinzawa-Itoh, K., Yoshikawa, S., Ogura, T.
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 14384-14385
  • Peer Reviewed
• [Journal Article] Iron-sulfur cluster N5 is coordinated by a HXXXCXXCXXXXXC motife in the NuoG subunit of E. coli NADH : quinine oxidoreductase (Complex I) (2008)
  • Author(s): Eiko Nakamura-Ogiso
  • Journal Title: J. Biol. Chem 283 Pages: 25979-25987
  • Peer Reviewed
• [Journal Article] Functional role of Coenzyme Q in the energy coupling of NADH-CoQ oxidoreductase (Complex I) : Stabilization of the semiquinone state with application of inside-positive membrane potential to proteoliposomes (2008)
  • Author(s): Tomoko Ohnishi
  • Journal Title: Biofctor 32 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Resonance Raman spectra of the FMN of the bovine heart NADH: coenzyme Q oxidoreductase, the largest membrane protein in the mitochondrial respiratory system (2007)
  • Author(s): Sugiyama, H., Nakatsubo, R., Yamaguchi, S., Ogura, T., Shinzawa-Itoh, K. Yoshikawa, S.
  • Journal Title: J. Bioenrg. Biomembr. 39 Pages: 145-148
  • Peer Reviewed
• [Journal Article] A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase (2007)
  • Author(s): Muramoto, K., Hirata, K., Shinzawa-Itoh, K., Yoko-o, S., Yamashita, E., Aoyama, H., Tsukihara, T., Yoshikawa, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 7881-7886
  • Peer Reviewed
• [Journal Article] The proton pumping pathway of bovine heart cytochrome c oxidase (2007)
  • Author(s): K. Shimokata, Y. Katayama, H. Murayama, M. Suematsu, T. Tsukihara, K. Muramoto, H. Aoyama, S. Yoshikawa, H. Shimada
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 4200-4205
  • Peer Reviewed
• [Journal Article] Structures and physiological roles of thirteen integral lipids of bovine heart cytochrome c oxidase (2007)
  • Author(s): K. Shinzawa-Itoh, H. Aoyama, K. Muramoto, H. Terada, T. Kurauchi, Y. Tadehara, A. Yamasaki, T. Sugimura, S. Kurono, K. Tsujimoto, T. Mizushima, E. Yamashita, T. Tsukihara, S. Yoshikawa
  • Journal Title: EMBO J. 26 Pages: 1713-1725
  • Peer Reviewed
• [Journal Article] A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase (2007)
  • Author(s): K. Muramoto, K. Hirata, K. Shinzawa-Itoh, S. Yoko-o, E. Yamashita, H. Aoyama, T. Tsukihara, S. Yoshikawa
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 7881-7886
  • Peer Reviewed
• [Journal Article] The proton pumping pathway of bovine heart cjrtochrome c oxidase (2007)
  • Author(s): Kunitoshi.Shimokata
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 4200-4205
  • Peer Reviewed
• [Journal Article] Structures and physiological roles of thirteen integral lipids of bovine heart c ochrome e oxidase (2007)
  • Author(s): Kyoko.Shinzawa-Itoh
  • Journal Title: EMBO J. 36 Pages: 1713-1725
  • Peer Reviewed
• [Journal Article] A Histidine residue acting as a controlling site for dioxygen reduction and roton pumping by cytochrome c oxidase (2007)
  • Author(s): Kazumasa.Muramoto
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 7881-7886
  • Peer Reviewed
• [Journal Article] Resonance Raman spectra of the FMN of the bovine heart NADH:ubiqvdnone oxidoreductase,the largest membrane protein in the mitochondrial respiratoy System (2007)
  • Author(s): Hidehisa.Sugiyama
  • Journal Title: J.Bioenerg.Biomembr. 39 Pages: 145-148
  • Peer Reviewed
• [Journal Article] A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase (2007)
  • Author(s): Muramoto, K., Hirata, K., Shinzawa-Itoh, K., Yoko-o, S., Yamashita,.E., Aoyama, H., Tsukihara, T., Yoshikawa, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA (In press)
• [Journal Article] Resonance Raman spectra of the FMN of the bovine heart NADH : ubiquinone oxidoreductase, the largest membrane protein in the mitochondrial respiratory system (2007)
  • Author(s): Sugiyama, H., Nakatsubo, R., Yamaguchi, S., Ogura, T., Shinzawa-Itoh, K., Yoshikawa, S.
  • Journal Title: J. Bioenergetics and Biomembranes (In press)
• [Journal Article] Structures and physiological roles of thirteen lipids of bovine heart cytochrome c oxidase (2007)
  • Author(s): Shinzawa-Itoh, K., Aoyama, H., Muramoto, K., Terada, H., Kurauchi, T., Tadehara, Y., Yamasaki, A., Sugimura, T., Kurono, S., Tsujimoto, K., Mizushima, T., Yamashita, E., Tsukihara, T., Yoshikawa, S.
  • Journal Title: EMBO J. 26 Pages: 1713-1725
• [Journal Article] The Proton-Pumping Pathway of Bovine Heart Cytochrome c Oxidase (2007)
  • Author(s): Shimokata, K., Katayama, Y., Murayama, H., Suematu, M., Tsukihara, T., Muramoto, K., Aoyama, H., Yoshikawa, S., Shimada, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 4200-4205
• [Journal Article] Reaction mechanism of bovine heart cytochrome c oxidase (2006)
  • Author(s): S. Yoshikawa, K. Muramoto, K. Shinzawa-Itoh, H. Aoyama, T, Tsukihara, T. Ogura, K. Shimokata, Y. Katayama, H. Shimnada
  • Journal Title: Biochim. Biophys. Acta 1757 Pages: 395-400
  • Peer Reviewed
• [Journal Article] Proton pumping mechanism of bovine heart cytochrome c oxidase (2006)
  • Author(s): S. Yoshikawa, K. Muramoto, K. Shinzawa-Itoh, H. Aoyama, T, Tsukihara, K. Shimokata, Y. Katayama, H. Shimnada
  • Journal Title: Biochim. Biophys. Acta 1757 Pages: 1110-1116
  • NAID: 110001154882
  • Peer Reviewed
• [Journal Article] Reaction mechanism of bovine heart cytochrome c oxidase (2006)
  • Author(s): Yoshikawa, S., Muramoto, K., Shinzawa-Itoh, K., Aoyama, H., Tsukihara, T., Ogura, T., Shimokata, K., Katayama, Y., Shimada, H
  • Journal Title: Biochim. Biophys. Acta 1757 Pages: 395-400
• [Journal Article] Proton pumping mechanism of bovine heart cytochrome c oxidase (2006)
  • Author(s): Yoshikawa, S., Muramoto, K., Shinzawa-Itoh, K., Aoyama, H., Tsukihara, T., Shimokata, K., Katayama, Y., Shimada, H.
  • Journal Title: Biochim. Biophys. Acta 1757 Pages: 1110-1116
  • NAID: 110001154882
• [Journal Article] Absolute configuration of the hydroxyfarnesylethyl group of haem A, determined by X-ray structural analysis of bovine heart cytopchyrome c oxidase using methods applicable at 2. 8 A resolution (2005)
  • Author(s): E. Yamashita(阪大), H. Aoyama(理研), M. Yao(理研), K. Muramoto, K. Shinzawa-Itoh, S. Yoshikawa, T. Tsukihara(阪大)
  • Journal Title: Acta Cryst. D61 Pages: 1373-1377
  • Peer Reviewed
• [Journal Article] Reaction mechanism and phospholipids structures of bovine heart cytochrome c oxidase (2005)
  • Author(s): S. Yoshikawa
  • Journal Title: Biochem. Soc. Trans. Pages: 934-937
  • Peer Reviewed
• [Journal Article] Probing the oxygen activation reaction in intact whole mitochondria through analysis of molecular vibrations (2005)
  • Author(s): T. Takahashi, S. Kuroiwa, T. Ogura, S. Yoshikawa
  • Journal Title: J. Am. Chem.. Soc. 127 Pages: 9970-9971
  • Peer Reviewed
• [Journal Article] Probing the oxygen activation reaction in intact whole mitochondria through analysis of molecular vibrations (2005)
  • Author(s): T.Takahashi, S.Kuroiwa, T.Ogura, S.Yoshikawa
  • Journal Title: J.Am.Chem.Soc. 127 Pages: 9970-9971
• [Journal Article] Mitochondrial cytochrome c oxidase (2005)
  • Author(s): S.Yoshikawa
  • Journal Title: Structures(B.Lewin ed.)(Virtual Text, Ergito Cambridge MA USA) 10 Pages: 201-205
• [Journal Article] Absolute configuration of the hydroxyfarnesylethyl group of haem A, determined by X-ray structural analysis of bovine heart cytochrome c oxidase using methods applicable at 2.8Å resolution (2005)
  • Author(s): E.Yamashita, H.Aoyama, M.Yao, K.Muramoto, K.Shinzawa-Itoh, S.Yoshikawa, T.Tsukihara
  • Journal Title: Acta Cryst D61 Pages: 1373-1377
• [Journal Article] Structura chemical studies on the reaction mechanism of cytochrome c oxidase (2005)
  • Author(s): S.Yoshikawa
  • Journal Title: Biophysical an Structural Aspects of Bioenrgetics(Marten Wikstrom ed.)(Royal Society of Chemistry, Cambridge UK.) Pages: 55-71
• [Journal Article] Reaction mechanism and phospholipids structures of bovine heart cytochrome c oxidase (2005)
  • Author(s): S.Yoshikawa
  • Journal Title: Biochem.Soc.Trans. 33 Pages: 934-937
• [Journal Article] Absolute configuration of the hydroxy-farnesylety group. (2005)
  • Author(s): Eiki Yamashita
  • Journal Title: Acta Cryst.D. (印刷中)
• [Journal Article] Structural chemical studies on the Reaction mechanism of cytochrome c oxidase. (2005)
  • Author(s): Shinya Yoshikawa
  • Journal Title: Biophysical and structural aspects of Bioenergetics Royal Society of Chemistry. UK (印刷中)
• [Journal Article] Mitochondrial cytochrome c oxidase (2005)
  • Author(s): Shinya Yoshikawa
  • Journal Title: Virtual Text (印刷中)
• [Journal Article] The intrinsic stability of the second intermediate following the dioxygen-bound form in the O2 reduction by cytochrome c oxidase (2004)
  • Author(s): K. Oda, T. Ogura, E.H. Appelman, S. Yoshikawa
  • Journal Title: FEBS Lett. 570 Pages: 161-165
  • Peer Reviewed
• [Journal Article] The intrinsic stability of the second intermediate following the dioxygen-bound form in the O_2 reduction by cytochrome c oxidase. (2004)
  • Author(s): Kenji Oda
  • Journal Title: EBS Lett. 570 Pages: 161-161
• [Presentation] Bovine heart cytochrome c oxidase structures facilitaiting the four-electron redeuction of O2 in one-step and the effective proton pump. (2010)
  • Author(s): S. Yoshikawa
  • Organizer: Invited Seminar in Department of Physiology and Biophysics, Albert Einstein College of Medicine
  • Place of Presentation: New York, NY, USA
  • Year and Date: 2010-01-07
• [Presentation] Bovine heart cytochrome c oxidase structures facilitaiting the four-electron redeuction of O_2 in one-step and the effective proton pump. Invited Seminar in Department of Physiology and Biophysics (2010)
  • Author(s): Shinya Yoshikawa
  • Organizer: Albert Einstein College of Medicine
  • Place of Presentation: New York, NY, USA
  • Year and Date: 2010-01-07
• [Presentation] チトクロム酸化酵素の02還元反応機構 (2009)
  • Author(s): 村本和優
  • Organizer: 日本生体エネルギー研究会第36回討論会
  • Place of Presentation: 旭川
  • Year and Date: 2009-12-18
• [Presentation] チトクロム酸化酵素のプロトンボンプ機構 (2009)
  • Author(s): 吉川信也
  • Organizer: 日本生体エネルギー研究会第36回討論会
  • Place of Presentation: 旭川
  • Year and Date: 2009-12-18
• [Presentation] 細菌チトクロムc酸化酵素の変異体解析が支持するプロトンポンプ経路の変異に対応したウシ心筋酵素部位の変異体解析 (2009)
  • Author(s): 網中良太
  • Organizer: 日本生体エネルギー研究会第36回討論会
  • Place of Presentation: 旭川
  • Year and Date: 2009-12-18
• [Presentation] シトクロムc-シトクロムc酸化酵素間電子移動反応の構造化学的解析 (2009)
  • Author(s): 坂本光一
  • Organizer: 日本生体エネルギー研究会第36回討論会
  • Place of Presentation: 旭川
  • Year and Date: 2009-12-18
• [Presentation] F_0F_1-ATP合成酵素の非共鳴ラマン研究 (2009)
  • Author(s): 三枝馨
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-01
• [Presentation] Paracoccus denitrificansチトクロム酸化酵素の大腸菌由来無細胞系での合成 (2009)
  • Author(s): 片山幸江
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-01
• [Presentation] 結晶中の完全酸化型ウシ心筋チトクロム酸化酵素の酸素還元中心の解析 (2009)
  • Author(s): 望月正雄
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-01
• [Presentation] シトクロムcにおけるシトクロムc酸化酵素との電子伝達反応に重要なアミノ酸残基の検討 (2009)
  • Author(s): 井上郁
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-01
• [Presentation] チトクロム酸化酵素の構造に基づく酸素の一段階4電子還元機構とプロトンの高効率ポンプ機構 (2009)
  • Author(s): 太田和宏
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] アジ化物イオン結合酸化型チトクロム酸化酵素の精密構造解析 (2009)
  • Author(s): 村本和優
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] チトクロム酸化酵素のF型中間体の再酸化反応による生成 (2009)
  • Author(s): 吉川信也
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] アミノ酸置換によるウシ心筋チトクロム酸化酵素の水素結合ネットワークの機能解析 (2009)
  • Author(s): 網中良太
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] チトクロムc酸化酵素におけるヒドロキシファルネシルエチル基の共鳴ラマンマーカーバンド (2009)
  • Author(s): 阪口美幸
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] チトクロム酸化酵素のCO光解離後の構造緩和過程の追跡 (2009)
  • Author(s): 石上泉
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] タンパク質機能の原子レベルの解明-序論-(シンポジウム、タンパク質機能の原子レベルの解明-X線構造、振動分光、分子生物学、理論解析による挑戦-) (2009)
  • Author(s): 吉川信也
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] チトクロム酸化酵素の部位特異的同位体標識(シンポジウム、タンパク質機能の原子レベルの解明 -X線構造、振動分光、分子生物学、理論解析による挑戦-) (2009)
  • Author(s): 島田秀男
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] チトクロム酸化酵素休止酸化型の1.4Å分解能X線構造解析 (2009)
  • Author(s): 前田和範
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] ウシ心筋NADH-ユビキノン酸化還元酵素2次元結晶の極低温電子顕微鏡試料の調製 (2009)
  • Author(s): 天野聡子
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] 共鳴ラマン分光法によるウシ心筋ミトコンドリア呼吸鎖NADH-ユビキノン酸化還元酵素のFMNと鉄-イオウクラスターの構造解析 (2009)
  • Author(s): 引田理英
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] ウシ心筋NADH-ユビキノン酸化還元酵素2次元結晶化 (2009)
  • Author(s): 島田悟
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] 共鳴ラマン分光法によるウシ心筋ミトコンドリア呼吸鎖NADH-ユビキノン酸化還元酵素のFMNと鉄-イオウクラスターの構造解析 (2009)
  • Author(s): 引田理英
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-20
• [Presentation] Structures of bovine cytochrome c oxidase facilitaiting the four-electron redeuction of O2 in one-step and the effective proton pump. (2009)
  • Author(s): S. Yoshikawa
  • Organizer: 14th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan, Plenary Lecture
• [Presentation] Mutagenesis studies on the functional roles of possible proton transfer pathways in bovine heart cytochrome c oxidase (2009)
  • Author(s): H. Shimada
  • Organizer: 14th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] Structure and dynamics of cytochrome c oxidase as studied with resonance Raman spectroscopy. (2009)
  • Author(s): T. Ogura
  • Organizer: 14th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] X-ray structures of CO, NO and Cyanide derivatives of bovine heart cytochrome c oxidase. (2009)
  • Author(s): S. Yoshikawa
  • Organizer: 54th National Meeting of the Italian Society of Biochemistry and Molecular Biology
  • Place of Presentation: Citta Universitatria, Catania Italy
• [Presentation] Structures of bovine cytochrome c oxidase facilitaiting the four-electron redeuction of O_2 in one-step and the effective proton pump (2009)
  • Author(s): Shinya Yoshikawa
  • Organizer: 14^<th> International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] Mutagenesis studies on the functional roles of possible proton transfer pathways in bovine heart cytochrome c oxidase (2009)
  • Author(s): OHideo Shimada
  • Organizer: 14^<th> Internationai Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] Structure and dynamics of cytochrome c oxidase as studied with resonance Raman spectroscopy (2009)
  • Author(s): Takashi Ogura
  • Organizer: 14^<th> International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] Time-resolved resonance Raman study on structural relaxation processof cytochrome c oxidase following photolysis of carbomnonoxide. (2009)
  • Author(s): Izumi Ishigami
  • Organizer: 14^<th> International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] A. new resonance Raman marker band of cytochrome c oxidase. (2009)
  • Author(s): Miyuki Sakaguchi
  • Organizer: 14^<th> International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] X-ray tructure and function of peroxide bridge between Fe and Cu in the O_2 reduction site of the fully oxidized cytochrome c oxldase (2009)
  • Author(s): Hiroshi Aoyama
  • Organizer: 14^<th> International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Japan
• [Presentation] X-ray structures of CO, NO and Cyanide derivatives of bovine heart cytochrome c oxidase (2009)
  • Author(s): Shinya Yoshikawa
  • Organizer: 54^<th> National Meeting of the Italian Society of Biochemistry and Molecular Biology, Citta Universitatria
  • Place of Presentation: Catania Italy
• [Presentation] ブタ心筋ミトコンドリア中のチトクロムc酸化酵素の共鳴ラマンスペクトル (2008)
  • Author(s): 柳沢幸子
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 水溶性の複合蛋白質Pseudomonas putidaチトクロムP450camの無細胞系での合成 (2008)
  • Author(s): 片山幸江
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] ウシ心筋チトクロム酸化酵幸のZn/Cd阻害部位のX線構造解析 (2008)
  • Author(s): 前田和範
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] チトクロム酸化酵素の結晶のSoret帯吸収スペクトル測定 (2008)
  • Author(s): 望月正雄
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] チトクロム酸化酵素二核中心に結合したCO, NO, CN^-の精密構造解析 (2008)
  • Author(s): 村本和優
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] チトクロム酸化酵素の高分解能の精密X線結晶構造解析 : 1.5A程度のX線回折データで膜蛋白質のプロトン化/脱プロトン化状態の決定方法の開発 (2008)
  • Author(s): 菅倫寛
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] ウシ心筋NADH-ユビキノン酸化還元酵素の2次元結晶化 (2008)
  • Author(s): 天野聡子
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] ウシ心筋NADH-ユビキノン酸化還元酵素の固有成分の分析 (2008)
  • Author(s): 小笠原由美
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 共鳴ラマン分光法によるウシ心筋NADH-ユビキノン酸化還元酵素のFMNと鉄硫黄クラスターの測定 (2008)
  • Author(s): 引田理英
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 還元型ウシ心筋チトクロム酸化酵素の配位子結合構造に基づくO_2還元機構 (2008)
  • Author(s): 村本和優
  • Organizer: 日本生体エネルギー研究会第34回討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-08
• [Presentation] シアン化物結合型チトクロム酸化酵素のX線結晶構造解析 (2008)
  • Author(s): 望月正雄
  • Organizer: 日本生体エネルギー研究会第34回討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] ウシチトクロム酸化酵素のプロトン輸送経路の変異体解析 (2008)
  • Author(s): 網中良太
  • Organizer: 日本生体エネルギー研究会第34回討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] チトクロム酸化酵素の無細胞発現系の構築 (2008)
  • Author(s): 島田秀夫
  • Organizer: 日本生体エネルギー研究会第34回討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] Reaction mechanism of bovine heart cytochrome c oxidase (2008)
  • Author(s): Shinya. Yoshikawa
  • Organizer: 53^<rd> National Meeting of Italian Society of Biochemis try and Molecular Biology
  • Place of Presentation: Riccione, Italy
  • Year and Date: 2008-09-26
• [Presentation] X-ray crystal structural analysis of cyanide binding cytochrome c oxidase (2008)
  • Author(s): Mochizuki M
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-26
• [Presentation] X-ray structure of carbon monoxide at copper site of the dinuclear site of cytochrome c oxidase (2008)
  • Author(s): Yoshikawa S
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-26
• [Presentation] X-ray structural analysis of Zinc/Cadmium inhibitory site in bovine heart cytochrome c oxidase (2008)
  • Author(s): Muramoto K
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-26
• [Presentation] High resolution diffraction experiment of bovine cytochrome c oxidase (2008)
  • Author(s): Suga M
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-26
• [Presentation] IPR beamline for macromolecular assemblies at SPring-8 (2008)
  • Author(s): Eiki Ymashita
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-26
• [Presentation] Proton Pumping Mechanism of Bovine Heart Cytochrome c Oxidase (2008)
  • Author(s): Shinya. Yoshikawa
  • Organizer: Evidence for H channel proton pump in bovine cytochrome c oxidase, 15^<th> European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2008-07-24
• [Presentation] High resolution X-ray diffraction experiment of bovine cytochrorne c oxidase (2008)
  • Author(s): Suga M
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2008-07-19
• [Presentation] X-ray structural analysis of zinc/cadmium inhibitory site in bovine heart cytochrome coxidase (2008)
  • Author(s): Muramoto K
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2008-07-19
• [Presentation] Mutations of possible proton-transfer pathways of bovine heart cytochrome c oxidase (2008)
  • Author(s): Hideo Shimada
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2008-07-19
• [Presentation] Proton Pumping Mechanism of Bovine Heart Cytochrome c Oxidase (2008)
  • Author(s): Shinya.Yoshikawa
  • Organizer: Joint Meeting of the Biophysical Society 52nd Annual Meeting and 16th International Biophysics Congress
  • Place of Presentation: Long Beach,California(USA)
  • Year and Date: 2008-02-02
• [Presentation] Lipid-Protein Interactions in Cytochrome c Oxidase (2008)
  • Author(s): Shinya.Yoshikawa
  • Organizer: Keystone Symposia, "Molecular Basis for Biological Membrane Organization"
  • Place of Presentation: Big sky Resort,Big Sky,Montana (USA)
  • Year and Date: 2008-01-13
• [Presentation] Reaction mechanism of bovine heart cytochrome c oxidase (2008)
  • Author(s): Yoshikawa, S.
  • Organizer: 53rd National Meeting of Italian Society of Biochemistry and Molecular Biology
  • Place of Presentation: Riccione, Italy
• [Presentation] Evidence for H channel proton pump in bovine cytochrome c oxidase (2008)
  • Author(s): Yoshikawa, S.
  • Organizer: 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] High resolution X-ray diffraction experiment of bovine cytochrome c oxidase (2008)
  • Author(s): Suga M
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] X-ray crystal structural analysis of cyanide binding cytochrome c oxidase (2008)
  • Author(s): Mochizuki M
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] A peroxide bridge between the two metals in the dinuclear center of the fully oxidized cytochrome c oxidase (2008)
  • Author(s): Aoyama H
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] X-ray structure of carbon monoxide at copper site of the dinuclear site of cytochrome c oxidase (2008)
  • Author(s): Muramoto K
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] Structural analyses for lipid/protein interactions in bovine heart cytochrome c oxidase (2008)
  • Author(s): Shinzawa-Itoh K, Aoyama H, Muramoto K, Terada H, Kurauchi T, Tadehara Y, Yamasaki A, Sugimura T, Kurono S, Tsujimoto K, Mizushima T, Yamashita E, Tsukihara T, Yoshikawa S.
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] X-ray structural analysis of zinc/cadmium inhibitory site in bovine heart cytochrome c oxidase (2008)
  • Author(s): Muramoto K
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] Synthesis of functional Paracoccus Denitrificans cytochrome c oxidase by Eschericia cali cell-free coupled transcription/translation system (2008)
  • Author(s): Y. Katayama
  • Organizer: The 15th European Bioenergetics Conference
  • Place of Presentation: Dublin, Ireland
• [Presentation] Mutations of possible proton-transfer pathways of bovine heart cytochrome c oxidase (2008)
  • Author(s): K, Shimokata
• [Presentation] Lipid-Protein Interactions in Cytochrome c Oxidase、Keystone Symposia (2008)
  • Author(s): Yoshikawa, S.
  • Organizer: Molecular Basis for Biological Membrane Organization
  • Place of Presentation: Big Sky, Montana USA
• [Presentation] Proton Pumping Mechanism of Bovine Heart Cytochrome c Oxidase, Joint Meeting of the Biophysical Society (2008)
  • Author(s): Yoshikawa, S.
  • Organizer: 52nd Annual Meeting and 16th International Biophysics Congress
  • Place of Presentation: Long Beach, California, USA
• [Presentation] ウシチトクロム酸化酵素の水形成用プロトン輸送経路D-pathwayの変異体解析 (2007)
  • Author(s): 下方 国稔
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] 酸化型ウシ心筋チトクロム酸化酵素のX線構造 (2007)
  • Author(s): 青山 浩
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] ミトコンドりア中のチトクロムc酸化酵素の構造解析:新たな&nu :(Fe-CO)ラマンモードの観測 (2007)
  • Author(s): 橋本 通子
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] ウシ心筋チトクロム酸化酵素の反応中間体のX線構造解析 (2007)
  • Author(s): 鍋倉 弘行
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] ウシ心筋チトクロム酸化酵素のZn阻害部位のX線構造解析 (2007)
  • Author(s): 村本 和優
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] 部分還元型ウシ心筋チトクロム酸化酵素のX線構造解析 (2007)
  • Author(s): 谷口 槙
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] シアン化物結合型チトクロム酸化酵素のX線結晶構造解析 (2007)
  • Author(s): 望月 正雄
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] Paracoccus denitirficans チトクロム酸化酵素遺伝子の大腸菌無細胞転写翻訳庫での発現に及ぼすYidC強制発現の効果 (2007)
  • Author(s): 片山 幸江
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
• [Presentation] 共鳴ラマン分光法によるウシ心筋ミトコンドリア呼吸鎖NADH:ユビキノン酸化還元酵素のFMNと鉄硫黄クラスターの測定 (2007)
  • Author(s): 森山 昌和
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
• [Presentation] ウシ心筋NADH-ユビキノン酸化還元酵素の2次元結晶化 (2007)
  • Author(s): 天野 聡子
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
• [Presentation] 代謝回転中のミトコンドリアの中のひとつの分子振動を観る (2007)
  • Author(s): 小倉 尚志
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
• [Presentation] Proton pumping mechanism of bovine heart cytochrome c oxidase (2006)
  • Author(s): S. Yoshikawa
  • Organizer: Bioenergetics Electron transport systems in biomembranes organized by S. Yoshikawa and W.A. Cramer
  • Place of Presentation: Kyoto, Japan
• [Presentation] Mutational analyses of a hydrogen bond network in a bacterial cytochrome c oxidase which is homologous to that of the bovine heart enzyme (2006)
  • Author(s): Y. Yukutake
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
• [Presentation] Reaction mechanism of bovine heart cytochrome c oxidase (2006)
  • Author(s): S. Yoshikawa
  • Organizer: The 14th European Bioenergetics Conference
  • Place of Presentation: Moscow Russia
• [Presentation] Mass spectral and X-ray structural analyses of lipids in bovine heart cytochrome c oxidase (2005)
  • Author(s): S. Yoshikawa
  • Organizer: BioScience 2005-from Gene to Systems
  • Place of Presentation: SECC, Glasgow, UK
• [Presentation] Experimental results indicating that heme a of bovine heart cytochrome c oxidase is the driving element of the proton-pumping (2005)
  • Author(s): S. Yoshikawa
  • Organizer: International Conference on Mitochondria, from Molecular Insight to Physiology and Pathology 40 Years of Bari Meetings
  • Place of Presentation: University of Bari, Bari Italy
• [Presentation] The low spin heme of cytochrome c oxidase as the driving element of the proton pumping process (2004)
  • Author(s): S. Yoshikawa
  • Organizer: 13th European Bioenergetics Conference, Colloquim ID, "Heme-Copper Oxygen Reductase"
  • Place of Presentation: Pisa, Italy
• [Book] Reducing dioxygen to water : cytochrome c oxidase : "Biological Inorganic Chemistry Structure and Reactivity"( edited by I. Bertini, H. B. Gray, E. I. Stiefel, J. S. Valentine) (2006)
  • Author(s): Yoshikawa, S.
  • Publisher: University Science Books, Sausalito, California
• [Book] Biophysical and Structural Aspects of Bioenrgetics(Marten Wikstrom ed. ) (2005)
  • Author(s): S. Yoshikawa
  • Publisher: Royal Society of Chemistry
• [Book] Virtual Text,Ergito Cambridge MA USA(B. Lewin ed., Mitochondrial cytochrome coxidase in "Structures") (2005)
  • Author(s): S. Yoshikawa