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Non-invasive MRI tracking of labeled stem cells and its application to regenerative medicine

Research Project

Project/Area Number 16100006
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionShiga University of Medical Science

Principal Investigator

INUBUSHI Toshiro  Shiga University of Medical Science, MR医学総合研究センター, 教授 (20213142)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Shigehiro  滋賀医科大学, MR医学総合研究センター, 准教授 (60220042)
遠山 育夫  滋賀医科大学, 分子神経科学研究センター, 教授 (20207533)
鳥居 隆三  滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
森田 将史  大阪大学, 免疫学フロンティア研究センター, 特任助教 (30381594)
加藤 雅也  石原産業株式会社, 中央研究所, 所長
近藤 靖  田辺製薬(株), 先端医学研究所, 主任研究員
Co-Investigator(Renkei-kenkyūsha) TOOYAMA Ikuo  滋賀医科大学, 分子神経科学研究センター, 教授 (20207533)
TORII Ryuzo  滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
MORITA Masahito  滋賀医科大学, MR医学総合研究センター, 客員助教 (30381594)
Research Collaborator KATO Masanari  石原産業株式会社, 中央研究所医薬研究所, 研究員
KONDO Yasushi  田辺製薬(株), 先端医学研究所, 主任研究員
Project Period (FY) 2004 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥111,930,000 (Direct Cost: ¥86,100,000、Indirect Cost: ¥25,830,000)
Fiscal Year 2008: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2007: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2006: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2005: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2004: ¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
KeywordsMRI / 幹細胞 / 分子イメージング / ナノ粒子 / 蛍光 / 近赤外 / 細胞標識 / 常時性鉄粒子 / 蛍光プローブ / 組胞画像 / 超常磁性鉄 / HVJ-E
Research Abstract

胚性幹(ES)細胞、ならびに、骨髄など成体から採取する幹細胞の分化・誘導技術の進展によって、再生医療への期待が高まっている。こうした新しい治療法を安全にかつ正確に遂行するには、治療の進行が的確に、かつ、連続的に確認でき、そして、治療の効果が正確に判定できる体内透視技術が必要となる。本研究では、臨床画像診断に用いられるMR画像法を元に移植細胞を生体内で可視化する技術を開発した。

Report

(6 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report

Research Products

(40 results)

All 2009 2008 2007 2006 2005 2004 Other

All Journal Article (29 results) (of which Peer Reviewed: 11 results) Presentation (5 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Trifluoromethoxy-benzylated ligands improve amyloid detection in the brain using (19)F magnetic resonance imaging2009

    • Author(s)
      Amatsubo T, Morikawa S, Inubushi T, Urushitani M, Taguchi H, Shirai N, Hirao K, Kato M, Morino K, Kimura H, Nakano I, Yoshida C, Okada T, Sano M, Tooyama I.
    • Journal Title

      Neurosci Res 63

      Pages: 76-81

    • NAID

      10027499428

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trifluoromethoxy-benzylated ligands improve amyloid detection in the brain using (19) F magnetic resonance imaging2009

    • Author(s)
      Amatsubo T, Morikawa S, et al.
    • Journal Title

      Neurosci Res. 63

      Pages: 76-81

    • NAID

      10027499428

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between high field MR images and histopathological findings of rat transplanted cancer immediately after partial microwave coagulation2008

    • Author(s)
      Murakami K, Morikawa S, et al
    • Journal Title

      Magn Eeson Med Sci. 7

      Pages: 105-112

    • NAID

      10024835746

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maturational changes in diffusion anisotropy in the rat corpus callosum : comparison with quantitative histological evaluation2008

    • Author(s)
      Jito J, Nakasu S, et al.
    • Journal Title

      J Magn Eeson Imaging. 28

      Pages: 847-854

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DJ-1 protects against neurodegeneration caused by focal cerebral ischemia and reperfusion in rats.2008

    • Author(s)
      Yanagisawa D
    • Journal Title

      J Cereb Blood Flow Metab. 28

      Pages: 563-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired peripheral circulation in lower-leg arteries caused by higher a rterial stiffness and greater vascular resistance associates with nephropathy in type 2 diabetic patients with normal ankle-brachial indices2008

    • Author(s)
      Yoshimura T
    • Journal Title

      Diabetes Res Clin Pract (電子版)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorine-19 fast recovery fast spin echo imaging for mapping 5-fluorouracil2007

    • Author(s)
      Morikawa S, Inubushi T, Morita M, Murakami K, Masuda C, Maki J, Tooyama I.
    • Journal Title

      Magn Reson Med Sci. 6(4)

      Pages: 235-40

    • NAID

      10020158662

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microglial transplantation increases amyloid-beta clearance in Alzheimer model rats2007

    • Author(s)
      Takata K, Kitamura Y, Yanagisawa D, Morikawa S, Morita M, Inubushi T, Tsuchiya D, Chishiro S, Saeki M, Taniguchi T, Shimohama S, Tooyama I
    • Journal Title

      FEBS Lett. 581(3)

      Pages: 475-8

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Magnetic resonance imaging using hemagglutinating virus of Japan-envelope vector successfully detects localization of intra-cardially administered microglia in normal mouse brain2007

    • Author(s)
      Song Y, Morikawa S, Morita M, Inubushi T, Takada T, Torii R, Tooyama I.
    • Journal Title

      Neurosci Lett. 395(1)

      Pages: 42-5

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microglial transplantation increases amyloid-beta clearance in Alzheimer model rats.2007

    • Author(s)
      Takata K
    • Journal Title

      FEBS Lett. 581

      Pages: 475-478

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microglial transplantation increases amyloid-beta clearance in Alzheimer model rats.2007

    • Author(s)
      Takata K, Kitamura Y, et al.
    • Journal Title

      FEBS Lett. 581・3

      Pages: 475-478

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The MR tracking of transplanted ATDC5 cells using fluorinated poly-L-lysine-CF3.2007

    • Author(s)
      Maki J, Masuda C, et al.
    • Journal Title

      Biomaterials 28・3

      Pages: 434-440

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of MR images and histochemical localization of intra-arterially administered microglia surrounding beta-amyloid deposits in the rat brain2006

    • Author(s)
      Song Y, Morikawa S, Inubushi T, Takada T, Torii R, Kitamura Y, Taniguchi T, Tooyama I.
    • Journal Title

      Histol Histopathol 21

      Pages: 705-711

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] I. MR tracking of transplanted glial cells using poly- L-lysine-CF3.2006

    • Author(s)
      Masuda C, Maki Z, et al.
    • Journal Title

      Neurosci Res 56・2

      Pages: 224-228

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A Low blood flow estimates in lower-leg arteries predict cardiovascular events in Japanese patients with type 2 diabetes with …2006

    • Author(s)
      Yoshimura T, Suzuki E, et al.
    • Journal Title

      Diabetes Care 29・8

      Pages: 1884-1890

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of MR images and histochemical localization of intra-art erially administered microglia surrounding beta-amyloid …2006

    • Author(s)
      Song Y, Morikawa S, et al.
    • Journal Title

      Histol Histopathol 21・7

      Pages: 705-711

    • Related Report
      2006 Annual Research Report
  • [Journal Article] N-Acetylaspartate concentrations in the thalami of neuropathic pain patients and healthy comparison subjects measured with (1)H-MRS.2006

    • Author(s)
      Fukui S, Matsuno M, et al.
    • Journal Title

      Magn Reson Imaging 24・1

      Pages: 75-79

    • Related Report
      2006 Annual Research Report
  • [Journal Article] N-Acetylaspartate concentrations in the thalami of neuropathic pain patients and healthy comparison subjects measured with (1)H-MRS.2006

    • Author(s)
      Fukui S, Matsuno M, et al.
    • Journal Title

      Magn.Reson.Imaging. 24・1

      Pages: 75-79

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Magnetic resonance imaging using hemagglutinating virus of Japan-envelope vector successfully detects localization …2006

    • Author(s)
      Song Y, Morikawa S, et al.
    • Journal Title

      Neurosci.Lett. 395・1

      Pages: 42-45

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Alternate biplanar MR navigation for microwave ablation of liver tumors.2005

    • Author(s)
      Sato K, Morikawa S, et al.
    • Journal Title

      Magn.Reson.Med.Sci. 4・2

      Pages: 89-94

    • NAID

      10016850799

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Abnormal peripheral circulation in type 2 diabetic patients with normal ankle-brachial index associates with coronary …2005

    • Author(s)
      Tsuchiya M, Suzuki E, et al.
    • Journal Title

      Diabetes Res.Clin.Pract. 70・3

      Pages: 253-262

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Assessment of the specific absorption rate and calibration of decouplingparameters for proton decoupled carbon-13 MR …2005

    • Author(s)
      Saito M, Matsuda T, et al.
    • Journal Title

      Eur.J.Radiol. 55・2

      Pages: 289-293

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Myocardial creatine concentration in various nonischemic heart diseases assessed by 1H magnetic resonance spectroscopy.2005

    • Author(s)
      Nakae I, Mitsunami k, et al.
    • Journal Title

      Circ.J. 69・6

      Pages: 711-716

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stiffness and impaired blood flow in lower-leg arteries are associated with severity of coronary artery calcification among asymptomatic type 2 diabetic2004

    • Author(s)
      Tsuchiya M, Suzuki E, et al.
    • Journal Title

      Diabetes Care. 27・10

      Pages: 2409-2415

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of metabolic abnormality in asynergic regions of ischemic human myocardium using 31P and 1H magnetic resonance spectroscopy.2004

    • Author(s)
      Nakae I, Mitsunami K, et al.
    • Journal Title

      J Cardiovasc Magn Reson. 6・3

      Pages: 685-696

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Magnetic resonance spectroscopic determination of a neuronal and axonal marker in white matter predicts reversibility of deficits in secondary normal2004

    • Author(s)
      Shiino A, Nishida Y, et al.
    • Journal Title

      J Neurol Neurosurg Psychiatry. 75・8

      Pages: 1141-1148

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Feasibility of respiratory triggering for MR-guided microwave ablation of liver tumors under general anesthesia.2004

    • Author(s)
      Morikawa S, Inubushi T, et al.
    • Journal Title

      Cardiovasc Intervent Radiol. 27・4

      Pages: 370-373

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effective magnetic labeling of transplanted cells with HVJ-E for magnetic resonance imaging.2004

    • Author(s)
      Toyoda K, Tooyama I, et al.
    • Journal Title

      Neuroreport 15・4

      Pages: 589-593

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Protective effects of moderate hypothermia on phosphoenergetic metabolism in rat liver during gradual hypoxia studied by in vivo 31P nuclear magnetic2004

    • Author(s)
      Takahashi K, Morikawa S, et al.
    • Journal Title

      J Surg Res. 117・2

      Pages: 323-328

    • Related Report
      2004 Annual Research Report
  • [Presentation] ナノプローブを用いるME分子イメージング2009

    • Author(s)
      犬伏俊郎
    • Organizer
      日本分子イメージング学会
    • Place of Presentation
      大宮ソニックシティー
    • Year and Date
      2009-05-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] MRIの分子イメージングへの応用2008

    • Author(s)
      犬伏俊郎
    • Organizer
      第2回創薬とイメージングに関するワークショップ
    • Place of Presentation
      大阪大学
    • Year and Date
      2008-12-22
    • Related Report
      2008 Final Research Report
  • [Presentation] 分子イメージング 生体画像法の新展開2008

    • Author(s)
      犬伏俊郎
    • Organizer
      第12回NMRマイクロイメージング研究会Keynote Lectures
    • Place of Presentation
      青山学院女子短期大学
    • Year and Date
      2008-07-26
    • Related Report
      2008 Final Research Report
  • [Presentation] ナノプローブを用いるMR分子イメージング2008

    • Author(s)
      犬伏俊郎
    • Organizer
      第3回日本分子イメージング学会 シンポジウム
    • Place of Presentation
      大宮
    • Year and Date
      2008-05-22
    • Related Report
      2008 Final Research Report
  • [Presentation] Bimodal Molecular Imaging with Magnetofluorescent Nanoparticle2007

    • Author(s)
      Inubushi T
    • Organizer
      日本分子イメージング学会
    • Place of Presentation
      フェニックスプラザ(福井)
    • Year and Date
      2007-06-28
    • Related Report
      2007 Annual Research Report
  • [Book] 進みつづける細胞移植治療の実際(田畑泰彦編集MRIによる細胞トレーシング)2008

    • Author(s)
      犬伏俊郎
    • Publisher
      メディカルドゥ
    • Related Report
      2008 Final Research Report
  • [Book] ますます広がる分子イメージング技術(佐治英郎, 田畑泰彦編 MRI分子イメージング-MRI分子プローブ)2008

    • Author(s)
      犬伏俊郎
    • Publisher
      メディカルドゥ
    • Related Report
      2008 Final Research Report
  • [Book] MRIで病気を診る-拓かれゆく画像診断の今日・明日-2008

    • Author(s)
      犬伏俊郎
    • Publisher
      ケイ・ディー・ネオブック
    • Related Report
      2008 Final Research Report
  • [Book] MRIで病気を診る-拓かれゆく画像診断の今日・明日-2008

    • Author(s)
      犬伏俊郎
    • Total Pages
      100
    • Publisher
      ケイ・ティー・ネオブック
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.shiga-med.ac.jp/-hqbioph/bmsc/index.htm

    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 球状金ナノ粒子組成物2007

    • Inventor(s)
      細見智栄、遠山育夫、犬伏俊郎、森川茂廣、山田弘志
    • Industrial Property Rights Holder
      国立大学法人 滋賀医科大学、株式会社アイ、エス、ティ
    • Industrial Property Number
      2007-338733
    • Filing Date
      2007-12-28
    • Related Report
      2008 Final Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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