Development of Measurement Methods and Analytical Reactions in Nanochemistry at the Liquid-Liquid Interfaces

• Project/Area Number: 16105002
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Osaka University
• Principal Investigator: WATARAI Hitoshi Osaka University, 大学院・理学研究科, 教授 (30091771)
• Co-Investigator(Kenkyū-buntansha): MONJUSHIRO Hideaki 大阪大学, 大学院・理学研究科, 准教授 (80191071) ; SUWA Masayori 大阪大学, 大学院・理学研究科, 助教 (90403097)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥112,710,000 (Direct Cost: ¥86,700,000、Indirect Cost: ¥26,010,000); Fiscal Year 2008: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2007: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2006: ¥30,290,000 (Direct Cost: ¥23,300,000、Indirect Cost: ¥6,990,000); Fiscal Year 2005: ¥40,950,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥9,450,000); Fiscal Year 2004: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
• Keywords: 液液界面吸着 / 界面集合体生成 / 界面キラル測定 / 界面SHG-CD / 界面磁気効果 / 界面単一分子反応 / 界面高速反応 / レーザー光泳動 / 液液界面磁化率 / 顕微円二色性 / 微粒子磁化率 / 界面分子集合体 / 共鳴ラマン散乱 / 全内部反射法 / 界面質量分析法 / 界面キラリティー / 界面キラル集合体生成 / 液液界面SHG / 界面キラル分子認識 / 遠心液膜円二色法 / 液液界面酵素触媒 / 界面タンパク機能診断 / 界面高速化学反応 / 遠心液膜法 / マイクロシースフロー法 / 界面電気泳動法 / 液液界面SHG-CD / 磁気泳動法

Research Abstract

液液界面における分子集合反応について、以下の成果を得た。
1)界面単一分子反応と高速界面反応の計測法、液液界面SERS法、遠心液膜円二色性(CD)法、液液界面SHG-CD法等の新手法を開発した。
2)界面単一分子ラクトン化反応、10^7-10^8M^<-1>s^<-1>の液液界面高速反応解析、液液界面におけるポルフィリンおよびフタロシアニンのキラル集合体の生成反応機構および界面吸着反応における磁場効果を明らかにした。

Research Products

• [Journal Article] OHDE, Takahiro Microscopic Measurement of Second Harmonic Generation from Chiral Surfaces (2009)
  • Author(s): TOKUNAGA, Daisuke; TAKECHI, Hideaki; YIN, Jian-Hua; WATARAI, Hitoshi
  • Journal Title: Anal. Sci. 25 Pages: 311-314
• [Journal Article] Magnetic susceptibility measurement of a microdroplet interface using a magnetic circuit. (2009)
  • Author(s): EGAMI, Shigeki; WATARAI, Hitoshi
  • Journal Title: The Analyst 134(2) Pages: 278-282
• [Journal Article] Effect of chloroform on complexation and chiral aggregation of bilirubin-bovine serum albumin at heptane/water interface. (2009)
  • Author(s): YIN, Jian-Hua; WATARAI, Hitoshi
  • Journal Title: J. Colloid Interface Sci. 329 Pages: 325-330
• [Journal Article] OYAMA, Hiroko In Situ Measurements of Aggregation and Disaggregation of Cu(II) Complex at Liquid/Liquid Interface. (2008)
  • Author(s): WATARAI, Hitoshi
  • Journal Title: Anal. Chem. 80(21) Pages: 8348-8353
• [Journal Article] Counterion-Dependent Morphology of Porphyrin Aggregates Formed at the Liquid/Liquid Interface Studied by Total Internal Reflection Resonant Rayleigh and Raman Scattering Microscopy. (2008)
  • Author(s): YAMAMOTO, Shigeki; WATARAI, Hitoshi
  • Journal Title: J. Phys. Chem. C 122 Pages: 12417-12424
• [Journal Article] Linear Dichroism of Zn(II)-TetrapyridylpoAggregates Formed at the Toluene/Water Interface. (2008)
  • Author(s): TAKECHI, Hideaki; ADACHI, Kenta; MONJUSHIRO, Hideaki; WATARAI, Hitoshi
  • Journal Title: Langmuir 24(9) Pages: 4722-4728
• [Journal Article] Microscopic Measurement of Circular Dichroism Spectra. (2008)
  • Author(s): MATSUGAKI, Aira; TAKECHI, Hideaki; MONJUSHIRO, Hideaki; WATARAI Hitoshi
  • Journal Title: Anal. Sci. 24 Pages: 297-300
  • NAID: 10021098731
• [Journal Article] Magnetophoretic Evaluation of Interfacial Adsorption of Dysprosium(III) on a Single Microdroplet. (2008)
  • Author(s): SUWA, Masayori; WATARAI, Hitoshi
  • Journal Title: Anal. Sci. 24(1) Pages: 133-137
  • NAID: 10020143704
• [Journal Article] Chiral complexation and aggregation of biliubin with serum albumin at a liquid/liquid interface. (2007)
  • Author(s): YIN, Jian-Hua; WATARAI, Hitoshi
  • Journal Title: Anal. Bioanal. Chem. 389(3) Pages: 895-902
• [Journal Article] Optical chirality of protonted tetraphenylporphyrin J-aggregate formed at the liquid/liquid interface in a certrifugal liquid membrane cell. (2007)
  • Author(s): WADA, Sayaka; FUJIWARA, Kazuhiko; MONJUSHIRO, Hideaki; WATARAI Hitoshi
  • Journal Title: J. Phys. Condens. Matter 19 Pages: 375105-375105
• [Journal Article] Effect of pulsed and static magnetic fields on surface tension measurement by drop volume method (2007)
  • Author(s): ISAI, Kento; SUWA, Masayori; WATARA Hitoshi
  • Journal Title: Bunseki Kagaku 56 Pages: 505-509
  • NAID: 110006291371
• [Journal Article] Resonance Raman spectroscopic study on chiral aggregation of bilirubin-bovine serum albumin complex formed at liquid/liquid interface. (2007)
  • Author(s): Yin, Jian-Hua; WATARAI Hitoshi
  • Journal Title: Anal. Sci. 23 Pages: 841-846
  • NAID: 10019526335
• [Journal Article] Role of Zn(II) and Pyridyl Groups in the Adsorption and Self-Aggregation of Zinc Tetrapyridylporphine at the liquid/liquid Interface. (2007)
  • Author(s): AKECHI, Hideaki; WATARAI, Hitoshi
  • Journal Title: Solvent Extraction Research and Development, Japan 14 Pages: 133-144
• [Journal Article] In situ measurement of undividual W/O microemulsions of aerosol OT in dodecane/wextraction system by total internal reflection lalight scattering microscopy (2007)
  • Author(s): TSUKAHARA, Satoshi; KITAGUCHI, Haruyasu; WATARAI, Hitoshi
  • Journal Title: chem. lett. 36(1) Pages: 148-149
• [Journal Article] Chiral complexation and aggregation of bilirubin with serum albumin at a liquid/liquid interface (2007)
  • Author(s): YIN, Jian-Hua, WATARAI, Hitoshi
  • Journal Title: Anal. Bioanal. Chem. 389(3) Pages: 895-902
  • Peer Reviewed
• [Journal Article] Optical chirality of protonated tetraphenylporphyrin J-aggregate for med at the liquid/liquid interface in a centrifugal liquid membrane cell (2007)
  • Author(s): WADA, Sayaka, FUJIWARA, Kazuhiko, MONJUSHIRO, Hideaki, WATARAI, Hitoshi
  • Journal Title: J. Phys. Condens. Matter 19 Pages: 375105-375105
  • Peer Reviewed
• [Journal Article] Resonance Raman spectroscopic study on chiral aggregation of bilirubin-bovine serum albumin complex formed at liquid/liquid interface (2007)
  • Author(s): Yin, Jian-Hua, WATARAI, Hitoshi
  • Journal Title: Anal. Sci. 23 Pages: 841-846
  • NAID: 10019526335
  • Peer Reviewed
• [Journal Article] (2007)
  • Author(s): WATARAI, Hitoshi edited by IMAE Toyoko
  • Journal Title: Measurement of complex formation and aggregation at the liquid-liquid interface. In "Advanced Chemistry of Monolayers at interfaces, 14"(Elsevier) Pages: 277-308
• [Journal Article] Optical chirality of protonated tetraphenylporphyrin J-aggregate formed at theliquid/liquid interface in a centrifugal liquid membrane cell (2007)
  • Author(s): WADA, Sayaka
  • Journal Title: J. Phys.: Condens Matter 19 Pages: 1-12
• [Journal Article] Measurement of hydrolysis kinetics galactose-substituted fluorescein by b-galactosidase at the toluene-water interface by spinning microtube fluorometry. (2006)
  • Author(s): OKAMOTO, Yusuke; MONJUSHIRO Hideaki; FUKUMOTO, Takao; WATARAI, Hitoshi
  • Journal Title: Anal. BioChem. 385(8) Pages: 1430-1438
• [Journal Article] Magnetic susceptibility measurements of solutions by surface nanodisplacement detection (2006)
  • Author(s): EGAMI, Shigeki; MONJUSHIRO, Hideaki; WATARAI, Hitoshi
  • Journal Title: Anal. Sci. 22(9) Pages: 1157-1162
  • NAID: 10018063956
• [Journal Article] Binding Behavior of Subphthalocyanine-Tagged Testosterone with Human Serum Albumin at the n-Hexane/Water Interface (2006)
  • Author(s): ADACHI, Kenta; WATARAI Hitoshi
  • Journal Title: Anal. Chem. 78(19) Pages: 6840-6846
• [Journal Article] Raman Microprobe Spectrometer Installed iSuper-Conducting Magnet (2006)
  • Author(s): GOTO, Takeyoshi; SUWA, Masayori; WATARAI Hitoshi
  • Journal Title: Anal. Sci. 22(7) Pages: 1043-1046
• [Journal Article] Spectroscopic Detection of Chiral Aggregation at Liquid-Liquid Interfaces (2006)
  • Author(s): WATARAI, Hitoshi; WADA, Sayaka;FUJIWARA, Kazuhiko
  • Journal Title: Tisinghua. Sci. Tech. 11 Pages: 228-232
• [Journal Article] Control of Optically Active Structure of Thioether-Phthalocyanine Aggregates by Chiral Pd(II)-BINAP Complexes in Toluene and at the Toluene/Water Interface. (2006)
  • Author(s): ADACHI, Kenta; CHAYAMA, Kenji; WATARAI, Hitoshi
  • Journal Title: Chirality 18(8) Pages: 599-608
• [Journal Article] Ion-Association Aggregation of an Anionic Porphyrin at the Liquid/Liquid Interface Studied by Second Harmonic Generation Spectroscopy (2006)
  • Author(s): FUJIWARA, Kazuhiko; WADA, Sayaka; MONJUSHIRO, Hideaki;WATARAI Hitoshi
  • Journal Title: Langmuir 22(6) Pages: 2482-2486
• [Journal Article] Electromagnetophoretic Measurements of Adsorption Forces of Polystyrene Microparticles on Silica Surfaces in Surfactant Solutions. (2006)
  • Author(s): IIGUNI, Yoshinori; WATARAI, Hitoshi
  • Journal Title: Bull Chem. Soc. Jpn. 79 Pages: 47-52
  • NAID: 40007080503
• [Journal Article] Formation of Helical J-Aggregate of Chiral Thioether-Derivatised Phthalocyanine Bound by Palladium(II) at the Toluene/Water Interface. (2006)
  • Author(s): ADACHI, Kenta; CHAYAMA, Kenji; WATARAI, Hitoshi
  • Journal Title: Langmuir 22(4) Pages: 1630-1639
• [Journal Article] Circular dichrodism measurement of (5, 10, 15, 20-tetrapyridylporphyrinato)zinc(II) at the toluene-water interface. (2006)
  • Author(s): MATSUMOTO, Yusuke; WATARAI, Hitoshi
  • Journal Title: Solvent Extraction Research and Development Japan 13 Pages: 207-211
• [Journal Article] Site-Selective Formation of Optically Active Inclusion Complexes of Alkoxo-Subphthalocyanines with beta-Cyclodextrin at the Toluene/Water Interface. (2006)
  • Author(s): ADACHI, Kenta; WATARAI, Hitoshi
  • Journal Title: Chem. Eur. J. 12(16) Pages: 4249-4260
• [Journal Article] Surface-Enhanced Raman Spectroscopy of Dodecanethiol-Bound Silver Nanoparticles at the Liquid/Liquid Interface (2006)
  • Author(s): YAMAMOTO, Shigeki;WATARAI Hitoshi
  • Journal Title: Langmuir 22(15) Pages: 6562-6569
• [Journal Article] WADA, Sayaka FUJIWARA, Kazuhiko Spectroscopic detection of chiral aggregation at liquid-liquid interfaces. (2006)
  • Author(s): WATARAI, Hitoshi
  • Journal Title: Tsinghua Science and Technology 11(2) Pages: 228-232
• [Journal Article] wo-phase Couette flow liner dichroism measurement of the shear-forced orientation of a palladium(II)-induced aggregate of thiother-derivatized subphthalocyanines at the toluene/glycerol interface (2006)
  • Author(s): ADACHI, Kenta; WATARAI, Hitoshi
  • Journal Title: New. J. Chem. 30(3) Pages: 343-348
• [Journal Article] Measurement of hydrolysis kinetics of galactose-substituted fluorescein by b-galactosidase at the toluene-water interface by spinning microtube fluorometry (2006)
  • Author(s): OKAMOTO, Yusuke
  • Journal Title: Anal. Bioanal. Chem. 385(8) Pages: 1430-1438
• [Journal Article] Binding Behavior of Subphthalocyanine-Tagged Testosterone with Human Serum Albumin at the n-Hexane/Water Interface (2006)
  • Author(s): ADACHI, Kenta
  • Journal Title: Anal. Chem. 78(19) Pages: 6840-6846
• [Journal Article] Control of Optically Active Structure of Thioether-Phthalocyanine Aggregates by Chiral Pd (II)-BINAP Complexes in Toluene and at the Toluene/Water Interface (2006)
  • Author(s): ADACHI, Kenta
  • Journal Title: Chirality 18(8) Pages: 599-608
• [Journal Article] Ion-Association Aggregation of an Anionic Porphyrin at the Liquid/Liquid Interface Studied by Second Harmonic Generation Spectroscopy (2006)
  • Author(s): FUJIWARA, Kazuhiko
  • Journal Title: Langmuir 22(6) Pages: 2482-2486
• [Journal Article] Site-Selective Formation of Optically Active Inclusion Complexes of Alkoxo-Subphthalocyanines with beta-Cyclodextrin at the Toluene/Water Interfaee (2006)
  • Author(s): ADACHI, Kenta
  • Journal Title: Chem. Eur. J. 12(16) Pages: 4249-4260
• [Journal Article] Surface-Enhanced Raman Spectroscopy of Dodecanethiol-Bound Silver Nanoparticles at the Liquid/Liquid Interface (2006)
  • Author(s): YAMAMOTO, Shigeki
  • Journal Title: Langmuir 22(15) Pages: 6562-6569
• [Journal Article] Magnetic susceptibility measurements of solutions by surface nanodisplacement detection (2006)
  • Author(s): EGAMI, Shigeki
  • Journal Title: Anal. Sci 22(9) Pages: 1157-1162
• [Journal Article] Raman Microprobe Spectrometer Installed in a Super-Conducting Magnet (2006)
  • Author(s): GOTO, Takeyoshi
  • Journal Title: Anal. Sci 22(7) Pages: 1043-1046
• [Journal Article] Electromagnetophoretic Measurements of Adsorption Forces of Polystyrene Microparticles on Silica Surfaces in Surfactant Solutions (2006)
  • Author(s): IIGUNI, Yoshinori
  • Journal Title: Bull. Chem. Soc. Jpn 79 Pages: 47-52
• [Journal Article] Formation of Helical J-Aggregate of Chiral Thioether-Derivatised Phthalocyanine Bound by Palladium (II) at the Toluene/Water Interface (2006)
  • Author(s): ADACHI, Kenta
  • Journal Title: Langmuir 22(4) Pages: 1630-1639
• [Journal Article] Circular dichroism measurement of (5,10,15,20-tetrapyridylporphyrinato) zinc (II) at the toluene-water interface (2006)
  • Author(s): MATSUMOTO, Yusuke
  • Journal Title: Solv. Extr. Research & Development, Japan 13 Pages: 207-211
• [Journal Article] Spectroscopic detection of chiral aggregation at liquid-liquid interfaces (2006)
  • Author(s): WATARAI, Hitoshi
  • Journal Title: Tsinghua Science and Technology 11(2) Pages: 228-232
• [Journal Article] Two-phase Couette flow linear dichroism measurement of the shear-forced orientation of a palladium(II)-induced aggregate of thioether-derivatized subphth alocyanines at the toluene/glycerol interface (2006)
  • Author(s): ADACHI, Kenta
  • Journal Title: New J. Chem 30(3) Pages: 343-348
• [Journal Article] Electromagnetophoretic Measurements of Adsorption Forces of Polystyrene Microparticles on Silica Surfaces in surfactant Solutions (2006)
  • Author(s): IIGUNI, Yoshinori
  • Journal Title: Bull.Chem.Soc.Jpn. 79巻1号 Pages: 47-52
• [Journal Article] Interfacial aggregation of thioether-substituted phthalocyaninatomagnesium(II)-Palladium(II) complexes in the toluene/water system (2005)
  • Author(s): ADACHI, Kenta
  • Journal Title: J.Mater.Chem., 15巻 Pages: 4701-4710
• [Journal Article] Aggregation of thioether-substituted subphthalocyanines with palladium(II) at the toluene-water interface (2005)
  • Author(s): ADACHI, Kenta
  • Journal Title: Soft Matter, 1巻(4号) Pages: 292-302
• [Journal Article] Measurement of dielectrophoretic mobility of single micro-particles in a flow channel. (2005)
  • Author(s): IKEDA, Ikuhiro
  • Journal Title: The Analyst 130巻(10号) Pages: 1340-1342
• [Journal Article] Lactone Cleavage Reaction Kinetics of Rhodamine Dye at Liquid/Liquid Interfaces Studied by Micro-Two-Phase Sheath Flow/Two-Photon Excitation Fluorescence Microscopy (2005)
  • Author(s): TOKIMTO, Takahira
  • Journal Title: Langmuir 21巻(4号) Pages: 1299-1304
• [Journal Article] Total Internal Reflection Second-Harmonic Generation Spectrometer System Optimized for the Liquid/Liquid Interface (2005)
  • Author(s): FUJIWARA, Kazuhiko
  • Journal Title: Rev.Sci.Instrum. 76巻(2号)
• [Journal Article] Interfacial hydrolysis in the μ-oxo dimer formation of tetraphenylpor phinatomanganese(III)in the toluene/water system (2004)
  • Author(s): K.Fujiwara, H.Watarai
  • Journal Title: Anal.Sci 20 Pages: 369-372
  • NAID: 10012047154
• [Journal Article] Interfacial aggregate growth process of Fe(II) and Fe(III) complexes with pyridylazophenol in solvent extraction system (2004)
  • Author(s): Y.Yulizar, H.Monjushiro, H.Watarai
  • Journal Title: J.Colloid Interface Sci 275 Pages: 560-569
• [Journal Article] Non-linear optical activity of porphyrin aggregate at the liquid/liquid interface (2004)
  • Author(s): K.Fujiwara, H.Monjushiro, H.Watarai
  • Journal Title: Chem.Phys.Lett 394 Pages: 349-353
• [Journal Article] Surface-enhanced Raman scattering from oleate-stabilized silver colloids at a liquid/liquid interface (2004)
  • Author(s): S.Yamamoto, K.Fujiwara, H.Watarai
  • Journal Title: Anal.Sci 20 Pages: 1347-1347
  • NAID: 10013524712
• [Journal Article] Dynamic microscopic extraction of europium(III) with 2-thenoyltrifluoroacetone observed as random fluorescence flashes at dodecane-water interface (2004)
  • Author(s): A.Takata, S.Tsukahara, H.Watarai
  • Journal Title: Chem.Lett 33(5) Pages: 518-519
  • NAID: 10012943301
• [Journal Article] Adsorption Equilibria of Novel phthalocyaninatomagnesium(III) Derivatives with Thioethers at the Toluene/Water Interface (2004)
  • Author(s): K.Adachi, H.Watarai
  • Journal Title: Bull.Chem.Soc.Jpn 77(11) Pages: 2011-2020
• [Presentation] Dynamic Force Analysis of Chemical Bonding with Electromagneto-Buoyancy (2009)
  • Author(s): H. Watarai, T. Kato
  • Place of Presentation: Nijmegen, Netherlands
• [Presentation] Chiral recognition by molecaggregation at liquid-liquid interface (2008)
  • Author(s): H. Watarai
  • Organizer: International Solvent Extraction Conference (ISEC2008)
  • Place of Presentation: Tucson, Arizona, USA
  • Year and Date: 2008-09-16
• [Presentation] Measurement of Optical Chirality at Liquid-Liquid Interface (2007)
  • Author(s): H. Watarai
  • Organizer: CSIXXXV
  • Place of Presentation: Xiamen
  • Year and Date: 2007-09-26
• [Presentation] Optical chirality analysis at liquid/liquid interface (2007)
  • Author(s): H. Watarai
  • Organizer: IUPAC Congress, Trino
  • Year and Date: 2007-08-10
• [Presentation] Optical chirality of molecular aggregates at liquid/liquid interface (2007)
  • Author(s): H. Watarai
  • Organizer: 233rd ACS National Meeting
  • Place of Presentation: Chicago
  • Year and Date: 2007-03-29
• [Book] Fundamentals in Solvent Extraction Processes Thermodynamic, Kinetic, and Interfacial Aspects. In "SOLVENT EXTRACTION and LIQUID MEMBRANES" (2008)
  • Author(s): H. Watarai.
  • Publisher: CRC press
• [Book] 分析化学 (2007)
  • Author(s): 渡會仁
  • Publisher: 丸善
• [Book] ナノテクのための化学・材料入門(ナノテクノロジー入門シリーズ2)日本表面科学会編集、Chapter 3局所構造:液液ナノ界面,固体界面,ナノ粒子 (2007)
  • Author(s): 山本茂樹, 飯国良規, 渡會仁, 本間芳和, 北森武彦
  • Publisher: 共立出版
• [Book] Measurement of complex formation and aggregation at the Liquid-Liquid interface. In“Advanced Chemistry of Monolayers at interfaces, 14" (2007)
  • Author(s): H. Watarai
  • Publisher: Elsevier
• [Book] 表面 (2006)
  • Author(s): ADACHI, Kenta
  • Total Pages: 20
  • Publisher: 広信社
• [Book] ぶんせき (2006)
  • Author(s): 渡會 仁
  • Total Pages: 7
  • Publisher: 日本分析化学会
• [Book] Interfacial Nanochmistry Molecular Science and Engineering at Liquid-Liquid Interfaces Series:Naostrucure Science and Technology (2005)
  • Author(s): Watarai, Hitoshi, Teramae, Norio, Sawada, Tsuguo (Eds.)
  • Publisher: Springer
• [Book] Interfacial Nanochemiatry Molecular Science and Engineering at Liquid-Liquid Interfaces (2005)
  • Author(s): H.Watarai, N.Teramae, T.Sawada
  • Total Pages: 321
  • Publisher: Kluwer Academic/Plenum Publishers
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/watarai/index.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/watarai/paper2.hte#2008