Studies on the signal transduction and the mechanism of chloroplast photorelocation movement

• Project/Area Number: 16107002
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: National Institute for Basic Biology (2005-2008); Tokyo Metropolitan University (2004)
• Principal Investigator: WADA Masamitsu 九州大学, 大学院理学研究院, 特任教授 (60011681)
• Co-Investigator(Kenkyū-buntansha): KANEGAE Takeshi 首都大学東京, 大学院理学研究科, 助教 (70264588) ; KIKUCHI Kazuhiro Cornell University, Boyce Thompson Institute, 博士研究員 (50332177)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥104,260,000 (Direct Cost: ¥80,200,000、Indirect Cost: ¥24,060,000); Fiscal Year 2008: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2007: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2006: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2005: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2004: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
• Keywords: 葉緑体 / 光定位 / 運動 / シロイヌナズナ / アクチン繊維 / phot / フォトトロピン / 葉緑体運動 / 光逃避運動 / アクチン / neo1 / シダ前葉体 / neol / 光定位運動 / Jドメイン / jac1タンパク質 / kac1タンパク質 / 葉緑体光定位運動 / シダ / 青色光 / 赤色光

Outline of Final Research Achievements

植物は効率良く光合成を行うために、光条件によって細胞内の葉緑体の分布を変えている。弱光に向かって集合し、強光からは逃避する。この現象を葉緑体光定位運動と呼ぶ。我々は葉緑体が光の強弱を感知する光受容体を以前に確定した。本研究では光受容後葉緑体がどのような機構で運動するかを調べ、関与する数種のタンパク質を同定するとともに、葉緑体が移動するために必須な、従来知られていなかったアクチン微繊維構造を発見した。

Research Products

• [Journal Article] Phototropins and neochromel mediate nuclear movement in the fern Adiantum capillus-veneris (2007)
  • Author(s): Tsuboi, H., N. Suetsugu, H. Kawai-Toyooka, M. Wada
  • Journal Title: Plant Cell Physiology 48 Pages: 892-896
  • Peer Reviewed
• [Journal Article] Fern as a model system to study photomorphogenesis (2007)
  • Author(s): Wada. M
  • Journal Title: Journal of Plant Research 120 Pages: 3-16
  • NAID: 10018828077
• [Journal Article] Phytochrome-dependent photomovement responses mediated by phototropin family proteins in Cryptogam plants (2007)
  • Author(s): Suetsugu, N., M. Wada
  • Journal Title: Photochem.Photobiol 83 Pages: 87-93
  • Peer Reviewed
• [Journal Article] The fern Adiantum capillus-veneris lacks stomatal responses to blue light (2006)
  • Author(s): Doi, M., M.Wada, K.Shimazaki
  • Journal Title: Plant Cell Physiol. 47 Pages: 748-755
  • NAID: 10017602239
• [Journal Article] A single chromoprotein with triple chromophores acts as both a phytochrome and a phototropin (2006)
  • Author(s): Kanegae, T, E.Hayashida, C.Kuramoto, M.Wada
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 17997-18001
• [Journal Article] An auxilin-like J-domain protein, JAC1, regulates phototropin-mediated chloroplast movement in Arabidopsis thalian (2005)
  • Author(s): Suetsugu, N., T.Kagawa, M.Wada
  • Journal Title: Plant Physiology 139 Pages: 151-162
• [Journal Article] Four distinct photoreceptors contribute to light-induced side branch formation in the moss Physcomitrella patens (2005)
  • Author(s): Uenaka, H., M.Wada, A.Kadota
  • Journal Title: Planta 222 Pages: 623-631
• [Journal Article] DNA interference : a simple and efficient gene-silencing system for high-throughput functional analysis in the fern Adiantum. (2004)
  • Author(s): Kawai-Toyooka, H., C.Kuramoto, K.Orui, K.Motoyama, K.Kikuchi, T.Kanegae
  • Journal Title: Plant Cell Physiol. 11 Pages: 1648-1657
  • NAID: 10014020751
• [Journal Article] Phototropins mediate blue and red light-induced chloroplast movements in Physcomitella patnes. (2004)
  • Author(s): Kasahara, M., T.Kagawa, Y.Sato, T.Kiyosue, M.Wada
  • Journal Title: Plant Physiology 113 Pages: 1388-1397
• [Journal Article] Chloroplast avoidance movement rate is fluence dependent. (2004)
  • Author(s): Kagawa, T., M.Wada
  • Journal Title: Photochemical & Photobiological Sciences 3 Pages: 592-595
• [Journal Article] Function analysis of Acphot2 using mutants deficient in blue light-induced chloroplast avoidance movement of the fern Adiantum capillus-veneris L. (2004)
  • Author(s): Kagawa, T., M.Kasahara, T.Abe, S.Yoshida, M.Wada
  • Journal Title: Plant Cell Physiology 45 Pages: 416-426
• [Journal Article] Positive and negative tropic curvature induced by microbeam irradiation of protonemal tip cells of the moss Ceratodon purpureus. (2004)
  • Author(s): Lamparter, T., T.Kagawa, G.Brucker, M.Wada
  • Journal Title: Plant Biology 6 Pages: 165-170
• [Journal Article] High pigmentlmutation negatively regulates phototropic signal transduction in tomato seedlings. (2004)
  • Author(s): Srinivas, A., RK Behera, T.Kagawa, M.Wada, R.Sharma
  • Journal Title: Plant Physiology 134 Pages: 790-800
• [Presentation] Chloroplast photorelocation movement:its photoperception and moving mechanisms (2007)
  • Author(s): Wada, M.
  • Organizer: International Conference of Plant Mitochondrial Biology 2007
  • Place of Presentation: Nara,Japan
• [Book] Light Sensing in Plants (2005)
  • Author(s): Wada, M., Shimazaki, K., Iino M.
  • Total Pages: 370
  • Publisher: Springer
• [Book] Light Sensing in Plants (2005)
  • Author(s): Wada, M.
  • Total Pages: 370
  • Publisher: Springer-Verlag, Tokyo