Control of embryogenesis of ascidians by localized mRNAs and embryonic induction

• Project/Area Number: 16107005
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Osaka University
• Principal Investigator: NISHIDA Hiroki Osaka University, 大学院・理学研究科, 教授 (60192689)
• Co-Investigator(Kenkyū-buntansha): KUMANO Gaku 大阪大学, 大学院・理学研究科, 助教 (80372605)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥104,650,000 (Direct Cost: ¥80,500,000、Indirect Cost: ¥24,150,000); Fiscal Year 2008: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2007: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2006: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2005: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2004: ¥33,800,000 (Direct Cost: ¥26,000,000、Indirect Cost: ¥7,800,000)
• Keywords: 胚発生 / 脊索動物 / ホヤ / 母性mRNA / 局在RNA / 胚誘導 / 繊維芽細胞成長因子 / 脊索 / 局在mRNA / 発生・発化 / 発生運命 / 発生・分化 / 母性因子 / 細胞間相互作用 / 原索動物

Research Abstract

ホヤは近年、発生過程の詳細な記述、ゲノムプロジェクトの完了などにより、発生生物学における新たなモデル動物として注目を集めている。本研究ではマボヤの初期発生を司るしくみの理解をさらに押し進めるため、卵内で局在しているmRNAについて詳細かつ網羅的な解析を行った。また、32細胞期あたりから始まる誘導的細胞間相互作用について、シグナルに対する応答能を制御する因子、そして誘導によって引き起こされる非対称細胞分裂の制御メカニズムを解析した。

Research Products

• [Journal Article] Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretzi. (2009)
  • Author(s): Niwano, T., Takatori, N., Kumano, G., Nishida, H.
  • Journal Title: Biol. Cell 101 Pages: 645-659
  • Peer Reviewed
• [Journal Article] Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by maternally localized (2009)
  • Author(s): Kumano, G., Nishida, H.
  • Journal Title: PEM. Dev. Biol. 331 Pages: 78-88
  • Peer Reviewed
• [Journal Article] Actin microfilaments guide the polarized transport of nuclear pore complexes and the cytoplasmic dispersal of Vasa mRNA during GVBD in the ascidian Halocynthia roretzi. (2009)
  • Author(s): Prodon, F., Hanawa, K., Nishida, H.
  • Journal Title: Dev. Biol. 330 Pages: 377-388
  • Peer Reviewed
• [Journal Article] Ascidians and the plasticity of the chordate developmental program. (2008)
  • Author(s): Lemaire, P., Smith, W.C., Nishida, H.
  • Journal Title: Curr. Biol. 18
  • Peer Reviewed
• [Journal Article] Cortical and cytoplasmic flows driven by actin microfilaments polarize the cortical ER-mRNA domain along the a-v axis in ascidian oocytes. (2008)
  • Author(s): Prodon, F., Sardet, C., Nishida, H.
  • Journal Title: Dev. Biol. 313 Pages: 682-699
  • Peer Reviewed
• [Journal Article] Cortical and cytoplasmic flows driven by actin microfilaments polarize the cortical ER-mRNA domain along the a-v axis in ascidian oocytes. (2008)
  • Author(s): Prodon, F.
  • Journal Title: Dev. Biol. 313 Pages: 682-699
  • Peer Reviewed
• [Journal Article] Localized PEM mRNA and protein are involved in cleavage plane orientation and unequal cell divisions in ascidians. (2007)
  • Author(s): Negishi, T., Takada, T., Kawai, N., Nishida, H.
  • Journal Title: Curr. Biol. 17 Pages: 1014-1025
  • Peer Reviewed
• [Journal Article] Direct activation by Ets and Zic is required for initial expression of the Brachyury gene in the ascidian notochord. (2007)
  • Author(s): Matsumoto, J., Kumano, G., Nishida, H.
  • Journal Title: Dev. Biol. 306 Pages: 870-882
  • Peer Reviewed
• [Journal Article] Cell fate polarization in ascidian mesenchyme/muscle precursors by directed FGF signaling and role for an additional ectodermal FGF antagonizing signal in notochord/nerve cord precursors (2007)
  • Author(s): Kim, G.J., Kumano, G., Nishida, H.
  • Journal Title: Development 134 Pages: 1509-1518
  • Peer Reviewed
• [Journal Article] Brain induction in ascidian embryos is dependent on juxtaposition of FGF9/16/20-producing and-receiving cells. (2007)
  • Author(s): Miyazaki, Y., Nishida, H., Kumano, G.
  • Journal Title: Dev. Genes Evol. 217 Pages: 177-188
  • Peer Reviewed
• [Journal Article] Localized PEM mRNA and protein are involved in cleavage plane orient ation and unequal cell divisions in ascidians. (2007)
  • Author(s): Negishi, T.
  • Journal Title: Current Biology 17 Pages: 1014-1025
  • Peer Reviewed
• [Journal Article] Cell fate polarization in ascidian mesenchyme/muscle precursors by directed FGF signaling and role for an additional ectodermal FGF antagonizing signal in notochord/nerve cord precursors. (2007)
  • Author(s): Kim, G. J.
  • Journal Title: Development 134 Pages: 1509-1518
  • Peer Reviewed
• [Journal Article] Direct activation by Ets and Zic is required for initial expression of the Brachyury gene in the ascidian notochord. (2007)
  • Author(s): Matsumoto, J.
  • Journal Title: Dev. Biol. 306 Pages: 870-882
  • Peer Reviewed
• [Journal Article] Brain induction in ascidian embryos is dependent on juxtaposition of FGF9/16/20-producing and-receiving cells. (2007)
  • Author(s): Miyazaki, Y.
  • Journal Title: Dev. Genes Evol. 217 Pages: 177-188
  • Peer Reviewed
• [Journal Article] Ascidian embryonic development: an emerging model system for the study of cell fate specification in chordates. (2007)
  • Author(s): Kumano, G.
  • Journal Title: Dev. Dynam. 236 Pages: 1732-1747
  • Peer Reviewed
• [Journal Article] FGF9/16/20 and Wnt-5α signals are involved in specification of secondary muscle fate in embryos of the ascidian, Halocynthia roretzi. (2007)
  • Author(s): Tokuoka, M.
  • Journal Title: Dev. Genes Evol. 217 Pages: 515-527
  • Peer Reviewed
• [Journal Article] Nuclear accumulation of β-catenin and transcription of downs tream genes are regulated by zygotic Wnt5 and maternal Dsh in ascidian embryos. (2007)
  • Author(s): Kawai, N.
  • Journal Title: Dev. Dynam. 236 Pages: 1570-1582
  • Peer Reviewed
• [Journal Article] Brain induction in ascidian embryos is dependent on juxtaposition of FGF9/16/20-producing and -receiving cells. (2007)
  • Author(s): Miyazaki, Y.
  • Journal Title: Dev. Genes Evol. (in press)
• [Journal Article] Ascidian embryonic development : an emerging model system for the study of cell fate specification in chordates. (2007)
  • Author(s): Kumano, G.
  • Journal Title: Dev. Dynam. (in press)
• [Journal Article] Extrinsic control of cell fate polarization by directed FGF signaling in mesenchyme/muscle precursors and involvement of an additional FGF antagonizing signal from ectoderm in notochord/nerve cord precursors in ascidian embryos. (2007)
  • Author(s): Kim, G. J.
  • Journal Title: Development (in press)
• [Journal Article] Overlapping expression of FoxA and Zic confers responsiveness to FGF signaling to specify notochord in ascidian embryos. (2006)
  • Author(s): Kumano, G., Yamaguchi, S., Nishida, H.
  • Journal Title: Dev. Biol. 300 Pages: 770-784
  • Peer Reviewed
• [Journal Article] The functional analysis of Type I postplasmic/PEM mRNAs in embryos of the ascidian Halocynthia roretzi. (2006)
  • Author(s): Nakamura, Y., Makabe, K.W., Nishida, H.
  • Journal Title: Dev. Genes Evol. 216 Pages: 69-80
  • Peer Reviewed
• [Journal Article] Overlapping expression of FoxA and Zic confers responsiveness to FGF signaling to specify notochord in ascidian embryos. (2006)
  • Author(s): Kumano, G.
  • Journal Title: Dev. Biol. 30 Pages: 770-784
• [Journal Article] The functional analysis of Type I postplasmic/PEM mRNAs in embryos of the ascidian Halocynthia roretzi. (2006)
  • Author(s): Nakamura, Y.
  • Journal Title: Dev.Genes Evol. 216 Pages: 69-80
• [Journal Article] POPK-1/Sad-1 kinase is required for the proper translocation of maternal mRNAs and putative germ plasm at the posterior pole of the ascidian embryo (2005)
  • Author(s): Nakamura, Y., Makabe, K.W., Nishida, H.
  • Journal Title: Development 132 Pages: 4731-4742
  • Peer Reviewed
• [Journal Article] Specification of embryonic axis and mosaic development in ascidians. (2005)
  • Author(s): Nishida, H.
  • Journal Title: Dev.Dynam. 233 Pages: 1177-1193
• [Journal Article] POPK-1/Sad-1 kinase is required for the proper translocation of maternal mRNAs and putative germ plasm at the posterior pole of the ascidian embryo. (2005)
  • Author(s): Nakamura, Y.
  • Journal Title: Development 132 Pages: 4731-4742
• [Journal Article] Regulation of NF-kB/Rel by IkB essential for ascidian notochord formation. (2005)
  • Author(s): Kawai, N.
  • Journal Title: Dev.Biol. 277 Pages: 80-91
• [Journal Article] Macho-1 functions as transcriptional activator for muscle formation in embryos of the ascidian Halocynthia roretzi. (2005)
  • Author(s): Sawada, K.
  • Journal Title: Gene Express. Patt. 5 Pages: 429-437
• [Journal Article] Tracing cell fate in brain formation during embryogenesis of the ascidian Halocynthia roretzi. (2004)
  • Author(s): Taniguchi, K., Nishida, H.
  • Journal Title: Dev. Growth Differ. 46 Pages: 163-180
  • NAID: 10012907895
  • Peer Reviewed
• [Journal Article] Ets-mediated brain induction in embryos of the ascidian (2004)
  • Author(s): Akanuma, T., Nishida. H.
  • Journal Title: Halocynthia roretzi. Dev. Genes Evol. 214 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Ets-mediated brain induction in embryos of the ascidian, Halocynthia roretzi. (2004)
  • Author(s): Akanuma, T.
  • Journal Title: Dev.Genes Evol. 214 Pages: 1-9
• [Journal Article] Tracing cell fate in brain formation during embryogenesis of the ascidian Halocynthia roretzi. (2004)
  • Author(s): Taniguchi, K.
  • Journal Title: Dev.Growth Differ. 46 Pages: 163-180
• [Presentation] How do embryos count the cell division rounds in each tissue lineage?: An ascidian case (2010)
  • Author(s): Hiroki Nishida
  • Organizer: 国際シンポジウム
  • Place of Presentation: Cell cycle and development(京都)
  • Year and Date: 2010-03-15
• [Presentation] Establishment of embryonic axes and regionalization along the axes: Polarity in organisms and cells. (2009)
  • Author(s): Hiroki Nishida
  • Organizer: 5th Interantional Tunicate Meeting
• [Presentation] Polarization of embryonic cells by prelocalized factors and external signals in ascidians. (2009)
  • Author(s): Hiroki Nishida
  • Organizer: The CDB Symposium 2009
  • Place of Presentation: "Shape and Polarity"(神戸)
• [Presentation] ホヤ胚発生における胚と細胞の極性化 (2008)
  • Author(s): 西田宏記
  • Organizer: 国際高等研究所セミナー
  • Place of Presentation: 奈良
  • Year and Date: 2008-12-06
• [Presentation] Cleavage pattern control and germ cell specification by localized maternal mRNA in ascidian embryos. (2008)
  • Author(s): Hiroki Nishida
  • Organizer: Swiss-Japan Joint Meeting
  • Place of Presentation: スイス Arosa
  • Year and Date: 2008-01-08
• [Presentation] マボヤ胚の前方と後方でFGFに対する応答能の違いを作り出す仕組み (2007)
  • Author(s): 熊野岳、西田宏記
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-20
• [Presentation] Cleavage pattern control and germ cell specification by localized maternal mRNA in ascidian embryos (2006)
  • Author(s): Hiroki Nishida
  • Organizer: Swiss-Japan Joint Meeting
  • Place of Presentation: Swiss, Arosa
• [Presentation] Notochord formation in ascidian embryos (2006)
  • Author(s): Hiroki Nishida
  • Organizer: 1st Meeting Italy-Japan on "VERTEBRATE ORGANOGENESIS"
  • Place of Presentation: Itary
• [Presentation] Role of localized maternal mRNAs in eggs of a basal chordate, the ascidian Halocynthia roretzi. (2005)
  • Author(s): Hiroki Nishida
  • Organizer: 15th International Congress of Developmental Biology
  • Place of Presentation: Sidney
• [Presentation] Culture, cleavage pattern, and gastrulation of Oikopleura dioica (2005)
  • Author(s): Hiroki Nishida
  • Organizer: 3rd International Tunicate Meeting
  • Place of Presentation: Santa Barbara
• [Presentation] Role of localized maternal mRNAs in eggs of a basal chordate, the ascidian Halocynthia roretzi (2005)
  • Author(s): Hiroki Nishida
  • Organizer: International Congress of Experimental Biology
  • Place of Presentation: San Diego
• [Book] 細胞周期フロンティア (2010)
  • Author(s): 西田宏記
  • Publisher: 共立出版(印刷中)
• [Book] 研究をささえるモデル生物 (2009)
  • Author(s): 西田宏記
  • Publisher: 化学同人
• [Book] 神経系の多様性:その起源と進化 (2007)
  • Author(s): 西田宏記
  • Publisher: 培風館
• [Book] 神経系の多様性:その起源と進化 (2007)
  • Author(s): 西田宏記, 他9名
  • Publisher: 培風館
• [Book] 神経系の多様性 : その起源と進化 (2007)
  • Author(s): 西田宏記
  • Publisher: 培風館(印刷中)
• [Book]
  • Author(s): http://www. bio. sci. osaka-u. ac. jp/bio_web/ lab_page/nishida/index. html
• [Remarks]
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/naiyou.html