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Development of rat models for human epilepsy by methods of gene modification

Research Project

Project/Area Number 16200029
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

SERIKWA Tadao  Kyoto University, Graduate School Medicine, Professor (30025655)

Co-Investigator(Kenkyū-buntansha) KURAMOTO Takashi  Kyoto University, Graduate School of Medicine, Associate Professor (20311409)
MASHIMO Tomoji  Kyoto University, Graduate School of Medicine, Research Associate Professor (80397554)
SHINOHARA Takashi  Kyoto University, Graduate School of Medicine, Professor (30322770)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥47,840,000 (Direct Cost: ¥36,800,000、Indirect Cost: ¥11,040,000)
Fiscal Year 2007: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2006: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2005: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2004: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
KeywordsRat / Epilepsy / Mutagenesis / Etyl nitrosourea / Disease model / Chlorambucil / Epilepsy gene / Gene-modified rat
Research Abstract

The aim of this study was the generation and partial characterization of mutant rats that show alteration in epilepsy related genes by screening of ENU- or chlorambucil-induced mutants. Although chlorambucil turned out to be not very efficient in our approach, a recessive mutation with a dwarf phenotype was obtained and is maintained by full-sib mating. We have furthermore developed a new, efficient approach that combines two methods : a high-throughput, low-cost screening assay that uses the phage Mu transposition reaction, and intracytoplasmic sperm injection (ICSI) for the recovery of the rare heterozygous genotypes from our newly generated frozen sperm repository, the Kyoto University Rat Mutant Archive (KURMA). This Mu transposition reaction can be combined with DNA pooling and therefore facilitates an efficient screening approach for mutagenized animals, termed MuT-POWER (Mu Transpositon Poling method With sequencer). The number of G1 DNA and sperm samples has already expanded to 5,000, and we could find 18 point mutations in epilepsy-related genes. Rats with a missense mutation in sodium channel SONIA gene were recovered from cryopreserved sperm by ICSI and a strain with the mutation has been generated. The rats showed a high sensitivity for pentylenetetrazol-induced convulsions and hot-water-induced convulsion, suggesting that they would be possible models for human heat-sensitive convulsions or GEFS+ patients. KURMA virtually allows the production of any rat model resembling human diseases.

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (72 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (53 results) (of which Peer Reviewed: 15 results) Presentation (16 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Progress and prospects in rat genetics: a community view.2008

    • Author(s)
      Aitman TJ, et. al.
    • Journal Title

      Nat Genet. 40(5)

      Pages: 516-522

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] AnENU-induced mutant archive for gene targeting in rats.2008

    • Author(s)
      Mashimo T, et. al.
    • Journal Title

      Nat Genet. 40(5)

      Pages: 514-515

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Progress and prospects in rat genetics : a community view2008

    • Author(s)
      Aitman, TJ., et. al.
    • Journal Title

      Nat Genet 40(5)

      Pages: 516-522

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] An ENU-induced mutant archive for gene targeting in rats2008

    • Author(s)
      Mashimo, T., et. al.
    • Journal Title

      Nat Genet 40(5)

      Pages: 514-515

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Progress and prospects in rat genetics : a community view.2008

    • Author(s)
      Aitman T, et. al.
    • Journal Title

      Nature Genet (in Press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An ENU-induced mutant archive for gene-targetting in rats.2008

    • Author(s)
      Mashimo T, et. al.
    • Journal Title

      Nature Genet (in Press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SNP and haplotype mapping for genetic analysis in the rat2008

    • Author(s)
      Saar K, et. al.
    • Journal Title

      Nature Genet (in Press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pancreatic metaplasia in the gastro-achlorhydria in WTC-dfk rat, a potassium channel Kcnql mutant.2008

    • Author(s)
      Kuwamura M, et. al.
    • Journal Title

      Vet Pathol (in Press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional polymorphisms in inbred rat strains and theirallele frequencies in commercially available outbred stocks.2008

    • Author(s)
      Kuramoto T, et. al.
    • Journal Title

      Physiol Genomics, (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of thymoma-prone congenic rat strain, ACI. BUF/Mna-Tsrl/Tsrl.2008

    • Author(s)
      Matsuyama M, et. al.
    • Journal Title

      J Cancer Res Clin Oncol. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of LEXF/FXLE rat recombinant inbred strains for the genetic dissection of complex traits.2008

    • Author(s)
      Voigt B, et. al.
    • Journal Title

      Physiol Genomics 32(3)

      Pages: 335-342

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of rat offspring derived from cryopreserved spermatozoa in Japanese National Bioresources2007

    • Author(s)
      Kashiwazaki N, et. al.
    • Journal Title

      Reprod Fertil Dev., 19(1)

      Pages: 124-125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Generation of rat offspring derived from cryopreserved spermatozoa in Japanese National Bioresources2007

    • Author(s)
      Kashiwazaki, N., et. al.
    • Journal Title

      Reprod Fertil Dev 19(1)

      Pages: 124-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Increased Ca2+ channel currents in cerebellar Purkinje cells of the ataxic groggy rat.2007

    • Author(s)
      Tanaka K, et. al.
    • Journal Title

      Neurosci Lett. 426(2)

      Pages: 75-80

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A comprehensive QTL analysis of serum cholesterol levels before and after a high-cholesterol diet in SHRSP.2007

    • Author(s)
      Mashimo T, et. al.
    • Journal Title

      Physiological Genomics 30(2)

      Pages: 95-101

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Voltage-dependent calcium channel abnormalities in hippocampal CA3 neurons of spontaneously epileptic rats.2007

    • Author(s)
      Yan HD, et. al.
    • Journal Title

      Epilepsia. 48(4)

      Pages: 758-764

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Absence-like and tonic seizures in Aspartoacylase/Attractin double-mutant mice.2007

    • Author(s)
      Gohma H, et. al.
    • Journal Title

      Exp Anim. 56(2)

      Pages: 161-165

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ataxic groggy rat has a missense mutation in the P/Q-type voltage gated Ca2+ channel <alpha>lA subunit gene and exhibits absence seizures.2007

    • Author(s)
      Tokuda S, et al.
    • Journal Title

      Brain Res 1133 (1)

      Pages: 168-177

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Generation of rat offspring derived from cryopreserved spermatozoa in Japanese National Bioresources.2007

    • Author(s)
      Kashiwazaki N, et al.
    • Journal Title

      Reprod Fertil Dev. 19 (1)

      Pages: 124-125

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination2006

    • Author(s)
      Shinohara T, et. al.
    • Journal Title

      Proc Natl Acad Sci USA 103(37)

      Pages: 13624-13628

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination2006

    • Author(s)
      Shinohara, T., et. al.
    • Journal Title

      Proc Natl Acad Sci USA 103(37)

      Pages: 13624-13628

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains.2006

    • Author(s)
      Mashimo T, et al.
    • Journal Title

      BMC Genetics 7

      Pages: 19-19

    • NAID

      120004770256

    • Related Report
      2006 Annual Research Report
  • [Journal Article] WTC deafness Kyoto (dfk) : a rat model for extensive investigations of Kcnql functions.2006

    • Author(s)
      Gohma H, et al.
    • Journal Title

      Physiol Genomics 24 (3)

      Pages: 198-206

    • Related Report
      2006 Annual Research Report
  • [Journal Article] WTC deafness Kyoto (dfk) : a rat model for extensive investigations of Kcnql functions.2006

    • Author(s)
      Gohma H, et al.
    • Journal Title

      Physiol Genomics 24(3)

      Pages: 198-206

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lanosterol synthase mutations cause cholesterol deficiency-associated cataracts in the Shumiya cataract rat.2006

    • Author(s)
      Mori M, et al.
    • Journal Title

      J Clin Invest 116

      Pages: 395-404

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anchorage-independent growth of mouse male germline stem cells in vitro.2006

    • Author(s)
      Kanatsu-Shinohara M, et al.
    • Journal Title

      Biol Reprod. 74(3)

      Pages: 522-529

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chromosomal mapping of hyperserum IgA and glomerular IgA deposition in a high IgA(HIGA) strain of DdY mice.2005

    • Author(s)
      Nogaki F, et al.
    • Journal Title

      Kidney Int. 68(6)

      Pages: 2517-2525

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Long-lasting antiepileptic effects of levetiracetam against epileptic sizures in spontaneously epileptic rat (SER) : differentiation of levetiracetam from conventional antiepileptic drugs.2005

    • Author(s)
      Ji-qun C, et al.
    • Journal Title

      Epilepsia 46(9)

      Pages: 1362-1370

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The rat pink-eyed dilution (p) mutation : An identical intragenic deletion in pink-eye dilute-coat strains and several Wistar-derived albino strains.2005

    • Author(s)
      Kuramoto T, et al.
    • Journal Title

      Mamm Genome 16

      Pages: 712-719

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Separation of Antiepileptogenic and Antiseizure Effects of Levetiracetam in the Spontaneously Epileptic Rat (SER).2005

    • Author(s)
      Yan HD, et al.
    • Journal Title

      Epilepsia 46(8)

      Pages: 1170-1177

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of AAV-2-mediated aspartoacylase gene transfer in the tremor rat model of Canavan disease.2005

    • Author(s)
      McPhee SW, et al.
    • Journal Title

      Brain Res Dev Brain Res. 27

      Pages: 112-121

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Restoration of aspartoacylase activity in CNS neurons does not ameliorate motor deficits and demyelination in a model of Canavan disease2005

    • Author(s)
      Klugmann M, et al.
    • Journal Title

      Mol Ther. 11(5)

      Pages: 745-753

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sparse and wavy hair : a new model for hypoplasia of hair follicle and mammary glands on rat chromosome 17.2005

    • Author(s)
      Kuramoto T, et al.
    • Journal Title

      J Hered. 96(4)

      Pages: 339-345

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mutation at the Lmxla locus provokes aberrant brain development in the rat.2005

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Brain Res Dev Brain Res. 155(2)

      Pages: 99-106

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Male germline stem cells : stem cells for germline modification and regenerative medicine.2005

    • Author(s)
      Shinohara T, et al.
    • Journal Title

      Seikagaku 77(11)

      Pages: 1409-1412

    • NAID

      10016886702

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic and epigenetic properties of mouse male germline stem cells during long-term culture.2005

    • Author(s)
      Kanatsu-Shinohara M, et al.
    • Journal Title

      Development. 74(3)

      Pages: 4155-4163

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Germline niche transplantation restores fertility in infertile mice.2005

    • Author(s)
      Kanatsu-Shinohara M, et al.
    • Journal Title

      Hum Reprod. 20(9)

      Pages: 2376-2382

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 特集 てんかん研究の新しい展開 てんかんモデルラット-遺伝性てんかんモデルの開発解析研究の現状と展望-2005

    • Author(s)
      徳田智子, 芹川忠夫
    • Journal Title

      神経研究の進歩 49(5)

      Pages: 656-664

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ラットフェノームプロジェクト:ラット既存系統の体系的特性評価2005

    • Author(s)
      真下知士, 他
    • Journal Title

      日本疾患モデル学会記録 21

      Pages: 17-21

    • NAID

      130000760626

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 遺伝子改変マウスの「迅速・簡便・安全」なジェノタイピング法2005

    • Author(s)
      庫本高志
    • Journal Title

      バイオテクノロジージャーナル 5(4)

      Pages: 461-436

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 実験用ラットの有用性-ナショナルバイオリソースプロジェクト「ラット」-2005

    • Author(s)
      真下知士, 他
    • Journal Title

      遺伝 59(3)

      Pages: 79-84

    • NAID

      40006736598

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis.2005

    • Author(s)
      Chuma S, et al.
    • Journal Title

      Development. Jan ; 132(1)

      Pages: 117-122

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic selection of mouse male germline stem cells in vitro : offspring from single stem cells.2005

    • Author(s)
      Kanatsu-Shinohara M, et al.
    • Journal Title

      Biol Reprod. Jan ; 72(1)

      Pages: 236-240

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rat Phenome Project : the untapped potential of existing rat strains.2005

    • Author(s)
      Mashimo T, et al.
    • Journal Title

      J Appl Physiol Jan ; 98(1)

      Pages: 371-379

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Generation of pluripotent stem cells from neonatal mouse testis.2004

    • Author(s)
      Kanatsu-Shinohara M, et al.
    • Journal Title

      Cell. 29 ; 119(7)

      Pages: 1001-1012

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transgenic mice Produced by retroviral transduction of male germline stem cells in vivo.2004

    • Author(s)
      Kanatsu-Shinohara M, et al.
    • Journal Title

      Biol Reprod Oct ; 71(4)

      Pages: 1202-1207

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rat genome sequencing and rat resources in Japan.2004

    • Author(s)
      Serikawa T.
    • Journal Title

      Tanpakushitsu Kakusan Koso. Oct ; 49(13)

      Pages: 2139-2148

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical and morphometrical studies on myelin breakdown in the demyelination (dmy) mutant rat.2004

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Brain Res. 1022(1-2)

      Pages: 110-116

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Antiepileptic effects of single and repeated oral administrations of S-312-d, a novel calcium channel antagonist, on tonic convulsions in spontaneously epileptic rats.2004

    • Author(s)
      Amano T, et al.
    • Journal Title

      J Pharmacol Sci. Jul ; 95(3)

      Pages: 355-362

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Colourful history of Japan's rat resources.2004

    • Author(s)
      Serikawa T.
    • Journal Title

      Nature. May6 ; 429(6987)

      Pages: 15-15

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Adenoviral gene transfer of aspartoacylase ameliorates tonic convulsions of spontaneously epileptic rats.2004

    • Author(s)
      Seki T.et al.
    • Journal Title

      Neurochem Int. Jul ; 45(1)

      Pages: 171-178

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Voltage-dependent Calcium Channel Abnormalities in Hippocampal CA3 Neurons of Spontaneously Epileptic Rats.

    • Author(s)
      Yan HD, et al.
    • Journal Title

      Epilepsia. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Absence-like and tonic seizures in Aspartoacylase/Attractin double-mutant mice.

    • Author(s)
      Gohma H, et al.
    • Journal Title

      Exp Anim (in press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] ナトリウムチャンネルScnla変異ラットの特性解析2007

    • Author(s)
      鶴見東志子, ら
    • Organizer
      第96回 関西実験動物研究会
    • Place of Presentation
      京都
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ENUミュータジェネシスにより作製したSCN1A遺伝子変異ラットの特徴について2007

    • Author(s)
      真下知士, ら
    • Organizer
      第3回 日本てんかん学会近畿地方会
    • Place of Presentation
      京都
    • Year and Date
      2007-11-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Gene-targeted rat models of human epilepsy efficiently developed by ENU mutagenesis.2007

    • Author(s)
      Mashimo T, et. al.
    • Organizer
      The 2007 meeting on Rat Genomics & Models
    • Place of Presentation
      Cold Spring Harbor
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Generation of rat offspring derived from cryopreserved spermatozoa in Japanese National Biorecources2007

    • Author(s)
      Kashiwazaki N, et. al.
    • Organizer
      The 33rd Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ENUミュータジェネシスにより作製されたScnla遺伝子変異ラット2007

    • Author(s)
      真下知士, ら
    • Organizer
      Neuro2007
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ENUミュータジェネシスによるSCN1A遺伝子変異ラットの開発2007

    • Author(s)
      真下知士, ら
    • Organizer
      第54回 日本実験動物学会総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Gene-targeted rat models of human epilepsy efficiently developed by ENU mutagenesis2007

    • Author(s)
      Mashimo, T., et. al.
    • Organizer
      The 2007 meeting on Rat Genomics & Models
    • Place of Presentation
      Cold Spring Harbor, New York, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 『ラット胚・精子の超低温保存と個体復元技術マニュアル(DVD版)』の作製2006

    • Author(s)
      滝澤明子, ら
    • Organizer
      第92回 関西実験動物研究会
    • Place of Presentation
      京都
    • Year and Date
      2006-12-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ENUミュータジェネシスによるラットリソース:新規てんかんモデルの開発2006

    • Author(s)
      真下知士, ら
    • Organizer
      第92回 関西実験動物研究会
    • Place of Presentation
      京都
    • Year and Date
      2006-12-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ENUミュータジェネシスによる新規ラットリソース資源2006

    • Author(s)
      真下知士, ら
    • Organizer
      第23回 日本疾患モデル学会
    • Place of Presentation
      渋川
    • Year and Date
      2006-12-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] MuT-POWER: A novel efficient screening method in gene-drivenENU mutagenesis2006

    • Author(s)
      Tokuda S, et. al.
    • Organizer
      Workshop on Immunology, Cancer, and Aging
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-02-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] MuT-POWER : A novel efficient screening method in gene-driven ENU mutagenesis2006

    • Author(s)
      Tokuda, S., et. al.
    • Organizer
      Workshop on Immunology, Cancer and Aging
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-02-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] MuT-POWER: a high-throughput DNA screening method for detecting ENU-induced mutations2006

    • Author(s)
      Mashimo T, et. al.
    • Organizer
      20th International Mouse Genome Conference,
    • Place of Presentation
      South Carolina
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] MuT-POWER: ENUミュータジェネシスにおける新規DNAスクリーニング法2006

    • Author(s)
      真下知士, ら
    • Organizer
      第53回 日本実験動物学会総会
    • Place of Presentation
      神戸
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] てんかんに関連する16遺伝子についてのラットSNP解析2006

    • Author(s)
      徳田智子, ら
    • Organizer
      第53回 日本実験動物学会総会
    • Place of Presentation
      神戸
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] MuT-POWER : a high-throughput DNA screening method for detecting ENU-induced mutations2006

    • Author(s)
      Mashimo, T., et. al.
    • Organizer
      20th International Mouse Genome Conference
    • Place of Presentation
      South Carolina
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.anim.med.kyoto-u.ac.jp/research.htm

    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 標的遺伝子中にミスマッチを有する生物のスクリーニング方法2006

    • Inventor(s)
      真下知士、柳原克彦、徳田智子、芹川忠夫
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      2006-003855
    • Filing Date
      2006-01-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 標的遺伝子中にミスマッチを有する生物のスクリーニング方法2006

    • Inventor(s)
      真下 知士, 他
    • Industrial Property Rights Holder
      京都大学
    • Filing Date
      2006-01-11
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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