| Budget Amount *help |
¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2006: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2005: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2004: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
|
|---|
| Research Abstract |
The present study was undertaken to examine natural products which have potential to reprogram somatic cells by using two procedures. 1. Screening of natural products by TG somatic cell lines; By using cell lines established from Oct4-GFP transgenic mice fetuses, natural products whose extracts have potential to induce Oct4 expression were examined. The water soluble extracts from natural products were freeze-dried, and after addition of each product at different concentrations to TG somatic cell lines, Oct4-GFP reaction was examined under fluorescence microscope. Of 506 products, 4 crud drugs showed positive reaction. Since the reaction was too weak, these crud drugs were extracted by methanol and the effects on the expression of Oct4-GFP in TG mouse somatic cells were examined. The positive reaction was observed in crud drugs Nos.212 and 368, but the reaction was weak and the expression of Oct4 was unclear at mRNA level. 2. Screening by preimplantation embryos; The natural products which have influences on the developmental potential or the timing of differentiation were examined. Although there were no products to enhance the developmental potential of zygotes to blastocysts, 5 crude drugs increased cell numbers of blastocysts more than 20%. Unexpectedly, 3 crude drugs had inhibitory effects on the developmental potential of zygotes even at 0.1 ug/ml. We are now examining whether such crude drugs have contraceptive reaction in animals.
|
