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Evaluation system of cardiac cells and tissues with combined micro-electrode array based electrical mapping and optical mapping system

Research Project

Project/Area Number 16200033
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Tokyo

Principal Investigator

SAKUMA Ichiro  The University of Tokyo, Department of Precision Engineering Graduate School of Engineering, Professor, 大学院工学系研究科, 教授 (50178597)

Co-Investigator(Kenkyū-buntansha) JIMBO Yasuhiko  The University of Tokyo, Department of Human and Engineered Environment Graduate School of Frontier Sciences, Professor, 大学院新領域創成科学研究科, 教授 (20372401)
MASAMUNE Etsuko  The University of Tokyo, Department of Precision Engineering Graduate School of Engineering, Associate Professor, 大学院工学系研究科, 助教授 (20345268)
KODAMA Itsuo  Nagoya University, Research Institute of Environmental Medicine, Professor, 環境医学研究所, 教授 (30124720)
LEE Jong-kook  Nagoya University, Research Institute of Environmental Medicine, Associate Professor, 環境医学研究所, 助教授 (60303608)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
Fiscal Year 2006: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2005: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2004: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Keywordsmicro-electrode array / optical mapping / regenerative medicine / arrhythmia / 細胞培養 / 光学測定 / 電極アレイ / 電気刺激 / 再生医療
Research Abstract

For progress of regenerative medicine in cardiology, such as bio-pacemaker based on cultured cardiac tissue for arrhythmia therapy, we need a system to evaluate function of cardiac cell, cardiac tissue, and a whole heart where the regenerated tissue is implanted. In this study, combined optical and electrical measurement system of cultured cardiac tissue and whole heart preparation has been developed.
1) We have developed a cardiac electric stimulation system with real time feedback of action potential propagation dynamics. In this system, cardiac electrical propagation is visualized by optical mapping method with high spatial and temporal resolution. Propagated excitation wave front is determined in real time, and the location and timing of electrical stimulation is controlled depending on the propagation pattern. It can initiate Make/Break excitation by controlling the timing and location considering the phase (depolarized, repolarized, and resting phase) of the cardiac tissue. The function of the system was confirmed by experiments with Langendorff perfused rabbit heart preparations.
2) We have also developed measurement system for cultured cardiac cells and stem cells where temporal and spatial electrical activation patterns are measured by an integrated micro-electrode arrays fabricated with photo lithography (64 channels, 30 μm X 30 μm, electrode spacing: 130 μm), and a fluorescence image of intracellular Ca is measured by a cooled CCD camera. Cardiac cells were separated from neonatal rats and cultured. mechanical contraction was observed after three days in vitro. It grows to contraction of extended areas of cultured cells. Synchronization of the electrical activities and mechanical contraction were also identified. Intracellular calcium concentration was measured by fluorescent dye (fluo-4) simultaneously. Synchronization of calcium dynamics and electrical activities were identified.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (22 results)

All 2007 2006 2005 2004

All Journal Article (21 results) Book (1 results)

  • [Journal Article] Mechanism of destabilization and early termination of spiral wave reentry in the ventricle by a class III antiarrhythmic agent,nifekalant2007

    • Author(s)
      Masatoshi Yamazaki, Haruo Honjo, Harumichi Nakagawa, Yuko S.Ishiguro, Yusuke Okubo, Mari Amino, Ichiro Sakuma, Kaichiro Kamiya, Itsuo Kodama
    • Journal Title

      Am Physiol Heart Circ Physiol 292

      Pages: 539-548

    • NAID

      10019861090

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mechanism of destabilization and early termination of spiral wave reentry in the ventricle by a class III antiarrhythmic agent, nifekalant2007

    • Author(s)
      Masatoshi Yamazaki, Haruo Honjo, Harumichi Nakagawa, Yuko S.Ishiguro, Yusuke Okubo, Mari Amino, Ichiro Sakuma, Kaichiro Kamiya, Itsuo Kodama
    • Journal Title

      Am Physiol Heart Circ Physiol 292

    • NAID

      10019861090

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The State of the Art of Nanobioscience in JApan2006

    • Author(s)
      Shoogo Ueno, Joji Ando, Hiroyuki Fujita, Tadashi Sugawara, Yasuhiko Jimbo, Keiji Itaka, Kazunori Kataoka, Takashi Ushida
    • Journal Title

      IEEE TRANSACTIONS ON NANOBIOSCIENCE 5

      Pages: 54-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The State of the Art of Nanobioscience in Japan2006

    • Author(s)
      Shoogo Ueno, Joji Ando, Hiroyuki Fujita, Tadashi Sugawara, Yasuhiko Jimbo, Keiji Itaka, Kazunori Kataoka, Takashi Ushida
    • Journal Title

      IEEE TRANSACTIONS ON NANOBIOSCIENCE 5

      Pages: 54-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Why Do Cardiac Arrhythmias Occur?--Experimental, In-Silico and Clinical Approach--2006

    • Author(s)
      Hiroshi Inada, Itsuo Kodama, Ichiro Sakuma, Kazuo Nakazawa
    • Journal Title

      CORONA PUBLITHING CO.,LTD.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 光学マッピングにおける心臓興奮伝播の動態解析2006

    • Author(s)
      高田, 山口, 荒船, 小林, 神保, 佐久間, 柴田, 本荘, 児玉
    • Journal Title

      生体医工学 44(in press)

    • NAID

      110004704980

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 心筋細胞活動電位数値計算モデルを用いた動物種によるIKrブロッカーに対する感受性の違いに関する検証2006

    • Author(s)
      山口, 荒船, 佐久間, 寺澤, 柴田, 本荘, 児玉, 神谷
    • Journal Title

      生体医工学 44(in press)

    • NAID

      130004493954

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 心筋細胞活動電位数値計算モデルを用いた動物種によるIKrブロッカーに対する感受性の違いに関する検証2005

    • Author(s)
      山口喬弘, 荒船龍彦, 佐久間一郎, 寺澤敏昭, 柴田仁太郎, 本荘晴朗, 児玉逸雄, 神谷香一郎
    • Journal Title

      生体医工学 43・4

      Pages: 605-615

    • NAID

      130004493954

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 光学マッピングにおける心臓興奮伝播の動態解析2005

    • Author(s)
      高田祐平, 山口喬弘, 荒船龍彦, 小林英津子, 神保泰彦, 佐久間一郎, 柴田仁太郎, 本荘晴朗, 児玉逸雄
    • Journal Title

      生体医工学 43・4

      Pages: 661-669

    • NAID

      110004704980

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 心筋細胞活動電子シミュレーションを用いたQT延長メカニズムの検討2005

    • Author(s)
      山口喬弘, 荒船龍彦, 佐久間一郎, 大内克洋, 柴田仁太郎, 本荘晴朗, 神谷香一郎, 児玉逸雄
    • Journal Title

      生体医工学 43・1

      Pages: 43-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of Differences in Sensitivity of Animals to an IKr Specific Bloker Using Simulation2005

    • Author(s)
      Takahiro Yamaguchi, Tatsuhiko Arafune, Ichiro Sauma, Toshiaki Terasawa, Nitaro Shibata, Haruo Honjo, Itsuo Kodama, Kaishiro KAmiya
    • Journal Title

      Transactions of Japanese Society for Medical and Biological Engineering Vol43(4)

      Pages: 605-615

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dynamic Analysis of Cardiac Excitation Propagation in Optical Mapping2005

    • Author(s)
      Yuhei Takata, Takahiro Yamaguchi, Tatsuhiko Arafune, Etsuko Kobayashi, Yasuhiko Jimbo, Ichiro Sakuma, Nitaro Shibata, Haruo Honjo, Itsuo Kodama
    • Journal Title

      Transactions of Japanese Society for Medical and Biological Engineering Vo143(4)

      Pages: 661-669

    • NAID

      110004704980

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2005

    • Author(s)
      Takahiro Yamaguchi, Tatsuhiko Arafune, Ichiro Sakuma, Katsuhiro Ouchi, Nitaro Shibata, Haruo Honjo, Kaichiro Kamiya, Itsuo Kodama
    • Journal Title

      Transactions of Japanese Society for Medical and Biological Engineering Vo143(1)

      Pages: 43-49

    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 心筋細胞活動電子シミュレーションを用いたQT延長メカニズムの検討2005

    • Author(s)
      山口, 荒船, 佐久間, 大内, 柴田, 本荘, 神谷, 児玉
    • Journal Title

      生体医工学 43

      Pages: 43-49

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons : evoked activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neurosci. 134

      Pages: 439-448

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons : spontaneous activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neurosci. 134

      Pages: 425-437

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stepwise pattern modification of neuronal network in photo-thermally-etched agarose architecture on multi-electrode array chip for single-cell-based electrophysiological measurement2005

    • Author(s)
      I.Suzuki, Y.Sugio, Y.Jimbo, K.Yasuda
    • Journal Title

      Lab Chip 5

      Pages: 241-247

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 心筋細胞活動電位シュミレーションを用いたQT延長メカニズムの検討2005

    • Author(s)
      山口, 荒船, 児玉, 佐久間他
    • Journal Title

      生体医工学 43(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Virtual Electrode-induced Spiral Reentry in Ventricular Myocardium Perfused in-vitro2004

    • Author(s)
      T.Arafune, A.Mishima, I.Sakuma, H.Inada, N.Shibata, H.Nakagawa, M.Yamazaki, H.Honjo, I.Kodama
    • Journal Title

      Environmental Medicine 47

      Pages: 72-75

    • NAID

      110004322471

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pattern Modification of a neuronal network for individual-call-based electrophysiol. measurement using photothermal etching of an agarose archtecture2004

    • Author(s)
      I.Suzuki, Y.Jimbo 他
    • Journal Title

      IEE Proc.Nanobiotchnol. 151

      Pages: 116-121

    • Related Report
      2004 Annual Research Report
  • [Journal Article] in viro系の多点電気刺激2004

    • Author(s)
      神保
    • Journal Title

      BME 18

      Pages: 43-47

    • Related Report
      2004 Annual Research Report
  • [Book] なぜ不整脈は起こるのか-心筋活動電位からスパイラルリエントリーまで-2006

    • Author(s)
      稲田紘, 児玉逸雄, 佐久間一郎, 中沢一雄
    • Total Pages
      240
    • Publisher
      コロナ社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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