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Evaluation of Genetic Diversity in and Molecular Genotyping of Asian Wheat Genetic Resources

Research Project

Project/Area Number 16201047
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Resource conservation science
Research InstitutionYokohama City University

Principal Investigator

SASANUMA Tsuneo (2006)  Yokohama City University, Kihara Institute for Biological Research, Assistant Professor, 木原生物学研究所, 助手 (70347350)

笹隈 哲夫 (2004-2005)  横浜市立大学, 木原生物学研究所, 教授 (70124310)

Co-Investigator(Kenkyū-buntansha) MIURA Hideho  Obihiro University of Agriculture and Veterinary Medicine, School of Veterinary and Medicine, Professor, 畜産学部, 教授 (10173981)
KAWAHARA Taihachi  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (20115827)
MORI Naoki  Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60230075)
KATO Kenji  Okayama University, Faculty of Agriculture, Professor, 農学部, 教授 (40161096)
笹沼 恒男  横浜市立大学, 木原生物学研究所, 助手 (70347350)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥45,240,000 (Direct Cost: ¥34,800,000、Indirect Cost: ¥10,440,000)
Fiscal Year 2006: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2005: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2004: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
KeywordsWheat / Aegilops / Landraces / Genetic diversity / Genotyping / Heading trait / Seed domancy / 遺伝資源 / 野生種・栽培腫 / 遺伝的多型 / 塩耐性 / 穂発芽 / 栽培植物の進化 / 野生種・栽培種
Research Abstract

Evaluation of genetic diversity in and molecular genotyping of genetic resources of wheat and its relatives were conducted. Modernization of agriculture has produced many modern wheat cultivars adapted with irrigation, chemical nutrition and mechanization, and today supported a huge world food demand. The modernization, however, resulted in loss of genetic diversity, thus we face the limitation of the increased yield and fragility against coming world environment changes. To overcome the problems, the genetic diversity present in wild species and are reconsidered, and the importance of evaluation and utilization of the potential genetic diversity are noticed. The establishment of the evaluation method of such diversity and the elucidation of the evolutionary process which has produced the diversity are also considered to be valuable.
The spread of cultivated wheat from its original place to the west and east produced a lot of differentiated local varieties, namely landraces, adapted wit … More h various local climates. Japanese wheat research community have collected wild species and landraces and have long-time conserved them as genetic resources. This research project is a collaborating research formed by researchers who have conserved the genetic resources and investigated genetic diversity or evolutionary processes. In this project approximately 600 cultivated and 700 wild accessions were investigated and the following outcomes were obtained.
1. The basic information of morphology, heading trait, salt tolerance and seed dormancy for each accession were evaluated and recorded. The level of diversity was considerably high in both wild and cultivated species.
2. Core collections (representative accessions covering whole population) were selected based on DNA marker and AFLP, and methods for conservation were proposed.
3. Multiple origins in both domestication and polyploidization processes were revealed, which are clearly contrasted with previous theories.
4. The genes controlling or related with heading trait and seed dormancy were identified using DNA marker. Genetic variation among accessions in those genes was revealed. These results enable us to use DNA marker to distinguish variety or to reveal evolutionary process.
5. The accessions used in this study and data obtained were registered in National BioResources Project (NBRP) database and free access and easy utilization were enabled. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (42 results)

All 2007 2006 2005 2004 Other

All Journal Article (41 results) Book (1 results)

  • [Journal Article] Allelic variation of heading trait-related genes, essential for wide adaptability of wheat, and its application to wheat breeding.2007

    • Author(s)
      Kato K.
    • Journal Title

      Gamma Field Symposia (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy.2007

    • Author(s)
      Nakamura S., Komatsuda T., Miura H.
    • Journal Title

      Theor. Appl. Genet. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mapping QTLs for grain dormancy on wheat 3A and group 4 chromosomes, and their combined effect.2007

    • Author(s)
      Mori M., Uchino N., Chono M., Kato K., Miura H.
    • Journal Title

      Theor.Appl.Genet. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Aegilops tauschii : genetic variation in Iran.2006

    • Author(s)
      Dudnikov A.J., Kawahara T.
    • Journal Title

      Genet. Resour. Crop Evol. 53

      Pages: 579-586

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic diversity and phylogenetic relationships among east Asian common wheat (Triticum aestivum L.) populations, revealed by the analysis of five isozymes.2006

    • Author(s)
      Ghimire S.K., Akashi Y., Masuda A., Washio T., Nishida H., Zhou Y.H., Yen C., Qi X., Li Z., Yoshino H., Kato K.
    • Journal Title

      Breed. Sci. 56

      Pages: 379-387

    • NAID

      110004863710

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ishii T., Arimura S., Ikeda N., Kamijima O., Mori N.
    • Journal Title

      Genes Genet. Syst. 81

      Pages: 211-214

    • NAID

      10018212800

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting tolerance QTLs.2006

    • Author(s)
      Kottearachchi N.S., Uchino N., Kato K., Miura H.
    • Journal Title

      Euphytica 152

      Pages: 421-428

    • NAID

      120006390186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 穂発芽耐性と種子休眠性の遺伝.2006

    • Author(s)
      三浦秀穂
    • Journal Title

      穂発芽-国産小麦の品質向上をめざして(穂発芽研究会編)

      Pages: 110-128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Variation and segregation for rachis fragility in spelt wheat, Triticum spelta L.2006

    • Author(s)
      Onishi I., Hongo A., Sasakuma T., Kawahara T., Kato K., Miura H.
    • Journal Title

      Genet. Resour. Crop Evol. 53

      Pages: 985-992

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. biuncialis and Ae. ovata.2006

    • Author(s)
      Sasanuma T., Kadosumi S., Kawahara T.
    • Journal Title

      Wheat Inf. Serv. 101

      Pages: 11-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pattern and rate of indel evolution inferred from whole chloroplast intergenic regions in sugarcane, maize and rice.2006

    • Author(s)
      Yamane K., Yano K., Kawahara T.
    • Journal Title

      DNA Research 13

      Pages: 197-204

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Aegilops tauschii : genetic variation in Iran.2006

    • Author(s)
      Dudnikov A.J., Kawahara T.
    • Journal Title

      Resour.Crop Evol. 53

      Pages: 579-586

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ghimire S.K., Akashi Y., Masuda A., Washio T., Nishida H., Zhou Y.H., Yen C., Qi X., Li Z., Yoshino H., Kato K.
    • Journal Title

      Breed.Sci. 56

      Pages: 379-387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ishii T., Arimura S., Ikeda N., Kamijima O., Mori N.
    • Journal Title

      Genes Genet.Syst. 81

      Pages: 211-214

    • NAID

      10018212800

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetics in tolerance to pre-harvest sprouting and seed dormancy. (in Japanese)2006

    • Author(s)
      Miura H.
    • Journal Title

      Pre-Harvest Dormancy-head for improvement of quality of domestic wheat

      Pages: 110-128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Variation and segregation for rachis fragility in spelt wheat, Triticum spelta L.2006

    • Author(s)
      Onishi I., Hongo A., Sasakuma T., Kawahara T., Kato K., Miura H.
    • Journal Title

      Genet.Resour.Crop Evol. 53

      Pages: 985-992

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. bluncialis and Ae. ovata.2006

    • Author(s)
      Sasanuma T., Kadosumi S., Kawahara T.
    • Journal Title

      Wheat Inf.Serv. 101

      Pages: 11-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Aegilops tauschii; genetic variation in Iran2006

    • Author(s)
      Dudnikov HJ, Kawahara T
    • Journal Title

      Genetic Resources and Crop Evolution 53

      Pages: 579-586

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increased grain dormancy in white-grained wheat by Introgression of preharvest sprouting tolerance QTLs2006

    • Author(s)
      Kottearachchi NS, Uchino N, Kato K, Miura T
    • Journal Title

      Euphytica 152

      Pages: 421-428

    • NAID

      120006390186

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pattern and rate of indel evolution inferred from whole chloroplast intergenic regions in sugarcane, maize and rice2006

    • Author(s)
      Yamane K, Yano K, Kawahara T
    • Journal Title

      DNA Reseaarch 13

      Pages: 197-204

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetic diversity and phylogenetic relationships among east Asian common wheat population revealed by the analysis of five Isozymes2006

    • Author(s)
      Ghimire SK, Akashi Y, Masuda A, Washio T, Nishida H, Zhou YH, Yen C, Qi X, Li Z, Yoshino H, Kato K
    • Journal Title

      Breeding Science 56

      Pages: 379-387

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ishii T, Arimura S, Ikeda N, Kojima O, Mori N
    • Journal Title

      Genes and Genetic Systems 81

      Pages: 211-214

    • NAID

      10018212800

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. biuncialis and Ae. ovata2006

    • Author(s)
      Sasanuma T, Kadosumi S, Kawahara T
    • Journal Title

      Wheat Information Service 101

      Pages: 11-12

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Variation and segregation for rachis fragility in spelt wheat, Triticum spelta L.2006

    • Author(s)
      Onishi, I., Hongo, A., Sasakuma, T., Kawahara, T., Kato, K., Miura, H.
    • Journal Title

      Genetic Resources and Crop Evolution 14

      Pages: 1-8

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic diversity and geographical differentiation in Asian common wheat (Triticum aestivum L.), revealed by the analysis of peroxidase and esterase isozymes.2005

    • Author(s)
      Ghimire S.K., Akashi Y., Maitani C., Nakanishi M., Kato K.
    • Journal Title

      Breed. Sci. 55

      Pages: 175-185

    • NAID

      10015669822

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. colomnaris and Ae. triaristata revealed based on the polyporphism of a genome-specific PCR fragment.2005

    • Author(s)
      Kadosumi S., Kawahara T., Sasanuma T.
    • Journal Title

      Genes Genet. Syst. 80

      Pages: 105-111

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 穂発芽耐性小麦の開発.2005

    • Author(s)
      三浦秀穂
    • Journal Title

      農林水産技術 研究ジャーナル 28 (7)

      Pages: 21-26

    • NAID

      10014351052

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mapping QTLs for grain dormancy on wheat 3A and group 4 chromosomes, and their combined effect.2005

    • Author(s)
      Mori M., Uchino N., Chono M., Kato K., Miura H.
    • Journal Title

      Theor. Appl. Genet. 110

      Pages: 1315-1323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Intra and interspecific phylogenetic relationships among diploid Triticum-Aegilops species based on base pair substitutions, indels and microsatellites in chloroplast non-coding sequences.2005

    • Author(s)
      Yamane K., Kawahara T.
    • Journal Title

      American J. Bot. 92

      Pages: 1887-1898

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic diversity and geographical differentiation in Asian common wheat (Triticum aestivum L.), revealed by the analysis of peroxidase and esterase isozymes.2005

    • Author(s)
      Ghimire S.K., Akashi Y., Maitani C., Nakanishi M., Kato K.
    • Journal Title

      Breed.Sci. 55

      Pages: 175-185

    • NAID

      10015669822

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. colomnaris and Ae. trIaristata revealed based on the polyporphism of a genome-specific PCR fragment.2005

    • Author(s)
      Kadosumi S., Kawahara T., Sasanuma T.
    • Journal Title

      Genes Genet.Syst. 80

      Pages: 105-111

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of wheat with tolerance to pre-harvest sprouting. (in Japanese)2005

    • Author(s)
      Miura H.
    • Journal Title

      Research Journal of Food and Agriculture 28

      Pages: 21-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Origin of U genome in two UM genome tetraploid Aegilops species, Ae. columnaris and Ae. triaristata, revealed based on the polymorphism of a genome-specific PCR fragment.2005

    • Author(s)
      Kadosumi, T.Kawahara, T., Sasanuma, T
    • Journal Title

      Genes and Genet.Systems 80

      Pages: 105-111

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mapping QTLs for grain dormancy on wheat 3A and group 4 chromosomes, and their combined effect2005

    • Author(s)
      Mori, M., Uchino, N., Chono, M., Kato, K., Miura H.
    • Journal Title

      Theoretical and Applied Genetics 111

      Pages: 156-168

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic analysis of photoperiodic response in wheat and its relation with the earliness of heading in southwestern part of Japan.2005

    • Author(s)
      M.Tanio, K.Kato, N.Ishikawa, Y.Tamura, M.Sato, H.Takagi, M.Matsuoka
    • Journal Title

      Breeding Science 55

      Pages: 327-334

    • NAID

      110001828201

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genealogical use for chloroplast DNA variation for intraspecific studies of Aegilops tauschii Coss.2005

    • Author(s)
      Y.Matsuoka, N.Mori, T.Kawahara
    • Journal Title

      Theoretical and Applied Genetics 111

      Pages: 265-271

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Variation and segregation for rachis fragility in spelt wheat, Triticum spelta L.2005

    • Author(s)
      Onishi, I., Hongo, A., Sasakuma, T., Kawahara, T., Kato.K., Miura, H.
    • Journal Title

      Genetic Resources and Crop Evolution (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mapping QTLs for grain dormancy on wheat 3A and group 4 chromosomes, and their combined effect2005

    • Author(s)
      Mori, M., Uchino, N., Chono, M., Kato, K., Miura, H.
    • Journal Title

      Theoretical and Applied Genetics (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Allelic variation at the VRN-1 promoter region in polyploid wheat.2004

    • Author(s)
      Yan L., Helguera M., Kato K., Fukuyama S., Sherman J., Dubcovsky J.
    • Journal Title

      Theor. Appl. Genet. 109

      Pages: 1677-1686

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chloroplast and nuclear DNA variation in common wheat : insight into the origin and evolution of common wheat.2004

    • Author(s)
      Hirosawa, S., S.Takumi, T.Ishii, T.Kawahara, C.Nakamura, N.Mori
    • Journal Title

      Genes and Genet.Systems 79(5)

      Pages: 271-283

    • NAID

      130000062124

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Allelic variation at the VRN-1 promoter region in polyploid wheat.

    • Author(s)
      Yan L., Helguera M., Kato K., Fukuyama S., Sherman J.
    • Journal Title

      Theor.Appl.Genet.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 雑誌「遺伝」特集:植物遺伝資源2004

    • Author(s)
      笹隈哲夫, 笹沼恒男等
    • Total Pages
      112
    • Publisher
      裳華房出版
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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