| Project/Area Number |
16206072
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Material processing/treatments
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKAI Osamu Nagoya University, EcoTopia Science Institute, Professor (40110712)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Nagahiro Nagoya University, Graduate School of Engineering, Associate Professor (00329096)
INOUE Yasushi Nagoya University, Ecotopia Scienm Institute, Associate Professor (10252264)
HOZUMI Atsushi National Institute of Advanced Industrial Science and Technology, Material Research Center of Sustainable Development, Researcher (40357950)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥49,790,000 (Direct Cost: ¥38,300,000、Indirect Cost: ¥11,490,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2005: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2004: ¥32,240,000 (Direct Cost: ¥24,800,000、Indirect Cost: ¥7,440,000)
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| Keywords | Scanning Probe Microscone / Nano-Bio / 走査プローブ顕微鏡 / 走査型プローブ顕微鏡 / ディップペンリソグラフィ / DNA / ケルビン力顕微鏡 / バイオ / リソグラフィー / ナノ / 化学反応 / 有機分子 |
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| Research Abstract |
Construction of the molecular device is one of the biggest aims in nanotechnology. Nanolithography is essential to the formulation of such a molecular device. We have focused on Scanning Probe Lithography (SPL) as one of such nanolithographic techniques. In this study, we attempted to immobilize biomolecules such as proteins in nano-domains by SPL.
At first, we have prepared surfaces with some surface characteristics by fabrication of self-ascembled monolayer (SAM) and investigated adsorption behaviors of biomolecules such as proteins on the SAMs. As a result, it was clarified that the surface wetting and surface potential, chemical affinity had great influences on the adsorption behavior of the biomolecules.
Next, we have fabricated micropatterned surfaces with two different domains in the surface characteristics(surface wetting, surface potential, chemical affinity)by light lithography and observed adsorption behavior of biomolecules on them. Then it was confirmed that the adsorbent adhered to the specific domain of the micro-pattern. We successfully controlled the immobilization of the adsorbents on the specific surface by the surface characteristics mentioned above.
Finally we have manufactured nanopatterned surfaces with different surface characteristics by SPL. Gold nanostructures on a silicon substrate were constructed by "Dip-Pen Nanolithography(DPN)"as one of SPL. Then, various kinds of frijol compounds were adsorbed on the structure body and surface characteristics of the nano-domain surface were controlled. We have successfully achieved position-selective immobilization of nanoparticles, as a model compound of a protein, and DNA.
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