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Analysis of different processes of gene transfer in aquatic ecosystems using the Aquatron

Research Project

Project/Area Number 16207001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionResearch Institute for Humanity and Nature (2005-2006)
Kyoto University (2004)

Principal Investigator

KAWABATA Zen'ichiro  Research Institute for Humanity and Nature, Research Department, Professor, 研究部, 教授 (80108456)

Co-Investigator(Kenkyū-buntansha) NASU Masao  Osaka University, Graduate School of Pharmacology, Professor, 大学院薬学研究科, 教授 (90218040)
ENDO Ginro  Tohokugakuin University, Faculty of Engineering, Professor, 工学部, 教授 (80194033)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
Fiscal Year 2006: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2005: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2004: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
KeywordsAquatron / Horizontal gene transfer / Natural transformation / Conjugation / Transduction / Microcystis aeruginosa bloom / Transposition / Phage / トランスポゾン / 接合性プラスミド / プランクトン群集 / 富栄養化 / 遺伝子伝播頻度 / 遺伝子伝播
Research Abstract

1 Bacterial DNAs which were released from their cells by phage infection are potential resources for gene transfer by natural transformation. Number of bacteria which took a dissolved plasmid with GFP gene in their cells by natural transformation is 100 times as great as that of bacteria which expressed GFP gene. This indicates natural transformation might take place in natural environments with a higher frequency than we have regarded so far.
2 Tetrahymena thermophila, a bacteriovore, inhibited a conjugal transfer from Escherichia coli to Pseudomonas stutzeri by predation on them, and the coexistence of T. thermophila with Microcystis aeruginosa, a producer, enhanced the inhibition.
3 A mini tranposon (mini Tn) was constructed by the transposon Tn MERI1 coding the mercury resistance genes. The mini Tn was introduced into a non-conjugal plasmid. Then this plasmid was transferred into Eshericia coli DH1with conjugal plasmid R338. It was observed the mini Tn transposed into R338 and then the mini Tn on R338 was transferred to Eshericia coli HB101 by conjugation. This suggested the possibility a conjugal transfer through transposition among replicon in a bacterial cell.
4 DNA sequences of the Tn MERI1 type transposon, IR and DR reiterated sequences on mercury resistance bacterial chromosome were analyzed. This analyses revealed that worldwide gene transfer by transposon took place among bacteria.
5 A community dominated with Microcystis aeruginosa, cyanobacteria, in the Aquatron reduced an infectivity of phage EC 10.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (45 results)

All 2007 2006 2005 2004

All Journal Article (36 results) Book (9 results)

  • [Journal Article] Long-term dynamics of catabolic plasmids introduced to a microbial community in a polluted environment : a mathematical model.2007

    • Author(s)
      Miki, T., Ueki, M., Kawabata, Z., Yamamura, N.
    • Journal Title

      FEMS Microbiology and Ecology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Viral abundance and its related factors in hypolimnion of a stratified lake.2007

    • Author(s)
      Honjo, M., Matsui, K., Ishii, N., Nakanishi, M., Kawabata, Z.
    • Journal Title

      Archiv fuer Hydrobiologie 168

      Pages: 105-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 有機水銀分解遺伝子と生物発光システムを用いた有機水銀化合物検出用微生物バイオセンサーの開発に関する研究.2007

    • Author(s)
      松井一彰, 遊佐清孝, 菅原宏幸, 成田勝, 遠藤銀朗
    • Journal Title

      水環境学会誌 30・2

      Pages: 77-81

    • NAID

      10018496801

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of bacterial biosensor for detecting organomercurials using organomercurial lyase gene and bioluminescence reporter system.2007

    • Author(s)
      Matsui, K., Yusa, K., Sugawara, H., Narita, M., Endo, G.
    • Journal Title

      Journal of Japan Society on Water Environment 30-2

      Pages: 77-81

    • NAID

      10018496801

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond.2006

    • Author(s)
      Honjo, M., Matsui, K., Ueki, M., Nakamura, R., Fuhrman, J.A., Kawabata, Z.
    • Journal Title

      Journal of Plankton Research 28

      Pages: 407-412

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative determination of free DNA uptake in river bacteria at the single cell level by in situ rolling circle amplification.2006

    • Author(s)
      Maruyama, F., Tani, K., Kenzaka, T., Yamaguchi, N., Nasu, M.
    • Journal Title

      Applied and Environmental Microbiology 72

      Pages: 6248-6256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A combination of direct viable counting, fluorescence in situ hybridization and green fluorescent protein gene expression for estimating plasmid transfer at the single cell level.2006

    • Author(s)
      Jorquera, M., Yamaguchi, N., Tani, K., Nasu, M.
    • Journal Title

      Microbes and Environments 21

      Pages: 101-111

    • NAID

      110004740910

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 蛍光染色による細菌の可視化と迅速・高精度検出.2006

    • Author(s)
      山口進康, 那須正夫
    • Journal Title

      日本細菌学雑誌 61

      Pages: 251-260

    • NAID

      10019168796

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 環境浄化微生物に見られる遺伝子の水平伝播-微生物種に共有された水銀耐性遺伝子から組み換え生物の開放系利用を考える.2006

    • Author(s)
      遠藤銀朗, 松井一彰, 成田勝
    • Journal Title

      環境バイオテクノロジー学会誌 6・1

      Pages: 27-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Direct visualization of bacteria with fluorescent staining methods.2006

    • Author(s)
      Yamaguchi, N., Nasu, M.
    • Journal Title

      Nihon Saikin Gakkaishi 61

      Pages: 251-260

    • NAID

      10019168796

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Horizontal gene transfer in environmental purification microorganisms- Consideration of the use of living modified organisms in the uncontained environments from the evidence of mercury resistance genes as microbial common properties.2006

    • Author(s)
      Endo, G., Matsui, K., Narita, M.
    • Journal Title

      Journal of Environmental Biotechnology 6-1

      Pages: 27-32

    • NAID

      40015603292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond.2006

    • Author(s)
      Honjo, M., et al.
    • Journal Title

      Journal of Plankton Research 28

      Pages: 407-412

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetic and physiological characterization of the intestinal bacterial microbiota of bluegill (Lepomis machrochirus) with three different feeding habits.2006

    • Author(s)
      Uchii, K., et al.
    • Journal Title

      Microbial Ecology 51・3

      Pages: 277-284

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quantitative determination of free DNA uptake in river bacteria at the single cell level by in situ rolling circle amplification.2006

    • Author(s)
      Maruyama, F., et al.
    • Journal Title

      Applied Environmental Microbiology 72

      Pages: 6248-6256

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A combination of direct viable counting, fluorescence in situ hybridization and green fluorescent protein gene expression for estimating plasmid transfer at the single cell level.2006

    • Author(s)
      Jorquera, M., et
    • Journal Title

      Microbes and Environmnts 21

      Pages: 101-111

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 蛍光染色による細菌の可視化と迅速・高精度検出2006

    • Author(s)
      山ロ進康 他
    • Journal Title

      日本細菌学雑誌 61

      Pages: 251-260

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 環境浄化微生物に見られる遺伝子の水平伝播-徹生物種に共有された水銀耐性遺伝子から組み換え生物の開放系利用を考える-2006

    • Author(s)
      遠藤銀朗 他
    • Journal Title

      環境バイオテクノロジー学会誌 6・1

      Pages: 27-32

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetic and physiological characterization of the intestinal bacterial microbiota of bluegill (Lepomis maclurochirus) with three different feeding hab2006

    • Author(s)
      Uchii, K., Matsui, K., Yonekura, R., Tani, K., Kenzaka, T., Nasu, M., Kawabata, Z.
    • Journal Title

      Microbial Ecology (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond2006

    • Author(s)
      Honjo, M., Matsui, K., Ueki, M., Nakamura, R., Fuhrman, J.A., Kawabata, Z.
    • Journal Title

      Journal of Plankton Research 28

      Pages: 407-412

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification.2005

    • Author(s)
      Maruyama, F., Kenzaka, T., Yamaguchi, N., Tani, K., Nasu, M.
    • Journal Title

      Applied and Environmental Microbiology 71

      Pages: 7933-7940

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.2005

    • Author(s)
      Kenzaka, T., Tamaki, S., Yamaguchi, N., Tani, K., Nasu, M.
    • Journal Title

      Applied and Environmental Microbiology 71

      Pages: 7236-7244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 自然生態系における細胞外DNAの動態と遺伝子伝播2005

    • Author(s)
      丸山史人, 谷佳津治, 那須正夫
    • Journal Title

      環境バイオテクノロジー学会誌 4

      Pages: 131-137

    • NAID

      40006735954

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Participation of the recA determinant in the transposition of class II transposon mini-TnMERI1.2005

    • Author(s)
      Matsui, K., Narita, M., Ishii, H., Endo, G.
    • Journal Title

      FEMS Microbiology Letters 253

      Pages: 309-314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 水俣湾底泥から分離されたBacillus属細菌由来のヒ素耐性トランスポゾンおよびそのarsオペロンの特徴に関する研究.2005

    • Author(s)
      佐藤充則, 黄介辰, 成田勝, 松井一彰, 遠藤銀朗
    • Journal Title

      東北学院大学環境防災工学研究所紀要 16

      Pages: 3-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dynamics and contributions to horizontal gene transfer of extracellular DNA in natural ecosystems.2005

    • Author(s)
      Maruyama, F., Tani, K., Nasu, M..
    • Journal Title

      Journal of Environmental Biotechnology 4

      Pages: 131-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Study on characteristics of an arsenic resistance transposon and flanked ars operon of Bacillus strain isolated from Minamata Bay sediment.2005

    • Author(s)
      Satoh, M., Huang, C-C., Narita M., Matsui, K., Endo, G.
    • Journal Title

      Bulletin of the Institute for Environmental and Disaster Prevention Engineering of Tohoku gakuin University 16

      Pages: 3-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Participation of the recA determinant in the transposition of class II transposon mini-TnMERI12005

    • Author(s)
      Matsui K., M.Narita, H.Ishii, G.Endo
    • Journal Title

      FEMS Microbiology Letters No.253

      Pages: 309-314

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The enhancement of conjugal plasmid pBHR1 transfer between bacteria in the presence of extracellular metabolic products produced by Microcystis aeruginosa.2004

    • Author(s)
      Ueki, M., Matsui, K., Choi, K., Z.Kawabata.
    • Journal Title

      FEMS Microbiology Ecology 51

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Release of transforming plasmid DNA from actively growing genetically engineered Escherichia coli.2004

    • Author(s)
      Ishii, N., Matsui, K., S.Fuma, S., Takeda, H., Kawabata, Z.
    • Journal Title

      FEMS Microbiology Letters 240

      Pages: 151-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Use of the SYBR Green I fluorescent dye and a centrifugal filter device for rapid determination of dissolved DNA concentration in fresh water.2004

    • Author(s)
      Matsui, K., Ishii, N., Honjo, M., Kawabata, Z.
    • Journal Title

      Aquatic Microbial Ecology 36

      Pages: 99-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dissemination of TnMERI1-like mercury resistance transposons among Bacillus isolated from worldwide environmental samples.2004

    • Author(s)
      Narita, M., Matsui, K., Ishii, H., Huang, C.-C., Kawabata, Z., Endo, G.
    • Journal Title

      FEMS Microbiology Ecology 48(1)

      Pages: 47-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The enhancement of conjugal plasmid pBHR1 transfer between bacteria in the presence of extracellular metabolic products produced by Microcystis aeruginosa2004

    • Author(s)
      Ueki, M., Matsui, K., Choi, K., Kawabata, Z.
    • Journal Title

      FEMS Microbiology Ecology 51

      Pages: 1-8

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Release pf transforming plasmid DNA from actively growing genetically engineered Escherichia coli2004

    • Author(s)
      Ishii, N., Matsui, K., Fuma, S., Takeda, H., Kawabata, Z.
    • Journal Title

      FEMS Microbiology Letters 240

      Pages: 151-154

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Use of the SYBER Green I fluorescent dye and a centrifugal filter device for rapid determination of disolved DNA concentration in fresh water2004

    • Author(s)
      Matsui, K., Ishii, N., Honjo, M., Kawabata, Z.
    • Journal Title

      Aquatic Microbial Ecology 36

      Pages: 99-106

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Trophic coupling of a testate amoeba and Microcystis species in a hypertrophic pond2004

    • Author(s)
      Nishibe, Y., Manage, P.M., Kawabata, Z., Nakano, S.
    • Journal Title

      Limnology 5

      Pages: 71-76

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dissemination of TnMERI-like mercury resistance transposons among Bacillus isolated from world wide environmental samples2004

    • Author(s)
      Narita, M., Matsui, K., Huang, C.C., Kawabata, Z., Endo, G.
    • Journal Title

      FEMS Microbiology Ecology 48

      Pages: 47-55

    • Related Report
      2004 Annual Research Report
  • [Book] 陸水の事典(日本陸水学会編)2006

    • Author(s)
      川端善一郎他
    • Total Pages
      574
    • Publisher
      講談社サイエンティフィック
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 病原微生物と人間の相互作用環、(日本微生物生態学会教育研究部会編)、微生物ってなに?p.184-194.2006

    • Author(s)
      川端善一郎
    • Total Pages
      204
    • Publisher
      日科技連
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 土と基礎の生態学.2.生態系の特性.54(10) : 30-36.2006

    • Author(s)
      川端善一郎
    • Publisher
      土と基礎
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Gene dynamics in natural aquatic ecosystems. Report of granted research,. p.20.2006

    • Author(s)
      Kawabata, Z.
    • Publisher
      The Asahi Glass Foundation
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 陸水の事典2006

    • Author(s)
      川端善一郎 他
    • Total Pages
      574
    • Publisher
      講談社サイエンティフィック
    • Related Report
      2006 Annual Research Report
  • [Book] 微生物ってなに?2006

    • Author(s)
      川端善一郎 他
    • Total Pages
      204
    • Publisher
      日科技遮
    • Related Report
      2006 Annual Research Report
  • [Book] 微生物生態学入門(日本微生物生態学会 教育研究部会 編)2004

    • Author(s)
      川端善一郎 他20名
    • Total Pages
      237
    • Publisher
      日科技連出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 微生物生態学入門(日本微生物生態学会 教育研究部会 編)2004

    • Author(s)
      松井一彰 他20名
    • Total Pages
      237
    • Publisher
      日科技連出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 微生物生態学入門2004

    • Author(s)
      川端 善一郎(共著)
    • Total Pages
      237
    • Publisher
      日科技連出版社
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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