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Molecular Mechanisms of Enhancement of Plant Growth by phototropins.

Research Project

Project/Area Number 16207003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionKYUSHU UNIVERCITY

Principal Investigator

SHIMAZAKI Ken-ichiro  Kyushu University, Department of Biology, Faculty of Science, Professor, 大学院理学研究院, 教授 (00124347)

Co-Investigator(Kenkyū-buntansha) DOI Michio  Kyushu University, Research and Development Center for Higher Education, Research Associate, 高等教育開発推進センター, 助手 (00167537)
KINOSHITA Toshinori  Kyushu University, Department of Biology, Faculty of Science, Research Associate, 大学院理学研究院, 助手 (50271101)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000)
Fiscal Year 2006: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2005: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2004: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
KeywordsStomata / Phototropin / Blue light effect / Photosynthesis / Growth / Plasma membrane H^+-ATPase / Chloroplast movement / 葉の横伸展 / 青色情報伝達
Research Abstract

Appropriate leaf positioning is essential for optimizing photosynthesis and plant growth. We show here the regulation of leaf positioning under blue light stimuli. When one-week-old Arabidopsis seedlings grown under white light were transferred to red light (25 μmol m-2 s-1) for 5 days, new petioles that appeared were almost horizontal and their leaves were curled and slanted downward. However, when a weak blue light from above (0.1 μmol m-2 s-1) was superimposed on red light, the new petioles grew obliquely upward and the leaves were flat and horizontal. The leaf positioning required both phototropin1 (phot1) and non-phototropic hypocotyl 3 (NPH3), and resulted in enhanced plant growth. In an nph3 mutant, neither optimal leaf positioning nor leaf flattening by blue light was found, and blue light-induced growth enhancement was drastically reduced. When blue light was increased from 0.1 μmol m-2 s-1 to 5 μmol m-2 s-1,normal leaf positioning and leaf flattening were induced in both phot1 and nph3 mutants, suggesting that phot2 signaling became functional and that the signaling was independent of phot1 and NPH3 in these responses. When plants were irradiated with blue light (0.1 μmol m-2 s-1) from the side and red light from above, the new leaves became oriented toward the source of blue light. We showed the tissue expression of NPH3 and its plasma membrane localization via the coiled-coil domain and the C-terminus region. We conclude that NPH3-mediated phototropin signaling optimizes the efficiency of light perception by inducing both optimal leaf positioning and leaf flattening, and enhances plant growth.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (30 results)

All 2007 2006 2005 2004 Other

All Journal Article (29 results) Book (1 results)

  • [Journal Article] Leaf positioning of Arabidopsis in response to blue light.2007

    • Author(s)
      Inoue et al.
    • Journal Title

      Mol. Plant (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Light regulation of stomatal movement.2007

    • Author(s)
      Shimazaki et al.
    • Journal Title

      Annu. Rev. Plant Biol. 58

      Pages: 219-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Phototropins and Blue Light-dependent Calcium Signaling in Higher Plants.2007

    • Author(s)
      Harada, Shimazaki
    • Journal Title

      Photochem.and Photobiol. 83

      Pages: 102-111

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 気孔の青色光応答2007

    • Author(s)
      武宮淳史, 井上晋一郎, 島崎研一郎
    • Journal Title

      蛋白質・核酸・酵素 増刊号 : 植物における環境と生物ストレスに対する応答 (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Leaf positioning of Arabidopsis in response to blue light.2007

    • Author(s)
      Inoue et al.
    • Journal Title

      Mol.Plant (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Light regulation of stomatal movement.2007

    • Author(s)
      Shimazaki et al.
    • Journal Title

      Annu.Rev.Plant Biol. 58

      Pages: 219-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 植物における環境と生物ストレスに対する応答2007

    • Author(s)
      武宮淳史, 井上晋一郎, 島崎研一郎
    • Journal Title

      蛋白質・核酸・酵素 増刊号

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cells.2007

    • Author(s)
      Zhang et al.
    • Journal Title

      Plant Cell Physiol (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Leaf positioning of Arabidopsis in responese to blue light.2007

    • Author(s)
      Inoue et al.
    • Journal Title

      Mol. Plant (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phototropins and Blue Light-dependent Calcium Signaling in Higher Plants.2007

    • Author(s)
      Harada, Shimazaki
    • Journal Title

      Photochem. and Photobiol 83

      Pages: 102-111

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 気孔の青色光応答2007

    • Author(s)
      武宮 淳史, 井上 晋一郎, 島崎 研一郎
    • Journal Title

      蛋白質・核酸・酵素 増刊号 : 植物における環境と生物ストレスに対する応答 (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The fern Adiantum capillus-veneris lacks stomatal responses to blue light.2006

    • Author(s)
      Doi et al.
    • Journal Title

      Plant Cell Physiol 47

      Pages: 748-755

    • NAID

      10017602239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Protein phosphatase 1 positively regulates stomatal opening in response to blue light in Vicia faba.2006

    • Author(s)
      Takemiya et al.
    • Journal Title

      PNAS 103

      Pages: 13549-13554

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 気孔の開口を駆動する細胞膜 H^+-ATPase2006

    • Author(s)
      木下俊則, 島崎研一郎
    • Journal Title

      蛋白質・核酸・酵素 51

      Pages: 871-876

    • NAID

      40007320097

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 気孔の開口を駆動する細胞膜H^+-ATPase2006

    • Author(s)
      木下俊則, 島崎研一郎
    • Journal Title

      蛋白質・核酸・酵素 51

      Pages: 871-876

    • NAID

      40007320097

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 気孔の開口を駆動する細胞膜H^+-ATPase2006

    • Author(s)
      木下 俊則, 島崎 研一郎
    • Journal Title

      蛋白質・核酸・酵素 51

      Pages: 871-876

    • NAID

      40007320097

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phototropin from Chlamydomonas reinhardtii is Functional in Arabidopsis thaliana.2005

    • Author(s)
      Onodera et al.
    • Journal Title

      Plant Cell Physiol 46

      Pages: 367-374

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Phototropins promote plant growth in response to blue light in low light environments.2005

    • Author(s)
      Takemiya et al.
    • Journal Title

      Plant Cell 17

      Pages: 1120-1127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Isolation of a Protein Interacting with Vfphot1a in Guard Cells of Vicia faba.2005

    • Author(s)
      Emi et al.
    • Journal Title

      Plant Physiol 138

      Pages: 1615-1626

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Biochemical characterization of the plasma membrane H^+-ATPase activation in guard-cell protoplasts of Arabidopsis thaliana in response to blue light.2005

    • Author(s)
      Ueno et al.
    • Journal Title

      Plant Cell Physiol 46

      Pages: 955-963

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Possible involvement of phototropins in leaf movement of kidney bean in response to blue light.2005

    • Author(s)
      Inoue et al.
    • Journal Title

      Plant Physiol 138

      Pages: 1994-2005

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Isolation of a Protein Interacting with Vfphotla in Guard Cells of Vicia faba.2005

    • Author(s)
      Emi et al.
    • Journal Title

      Plant Physiol 138

      Pages: 1615-1626

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phototropins promote plant growth in response to blue light in low light environments.2005

    • Author(s)
      Takemiya et al.
    • Journal Title

      Plant Cell (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant.2004

    • Author(s)
      Doi et al.
    • Journal Title

      J Exp Bot. 55

      Pages: 517-523

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Abscisic acid induces rapid subnuclear reorganization in guard cells.2004

    • Author(s)
      Ng et al.
    • Journal Title

      Plant Physiol 134

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.2004

    • Author(s)
      Shimaoka et al.
    • Journal Title

      Plant Cell Physiol 45

      Pages: 672-683

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Inhibition of blue light-dependent H^+ pumping by abscisic acid through hydrogen peroxide-induced dephosphorylation of the plasma membrane H^+-ATPase in guard cell protoplasts.2004

    • Author(s)
      Zhang et al.
    • Journal Title

      Plant Physiol 136

      Pages: 4150-4158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] A transgene encoding a blue-light receptor, phot1,restores blue-light responses in the Arabidopsis phot1 phot2 double mutant.2004

    • Author(s)
      Doi et al.
    • Journal Title

      Journal of Experimental Botany 55

      Pages: 517-523

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 気孔の青色光応答

    • Author(s)
      武宮淳史, 井上晋一郎, 島崎研一郎
    • Journal Title

      蛋白質・核酸・酵素 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Light Sensing in Plants2005

    • Author(s)
      Wada et al.
    • Total Pages
      370
    • Publisher
      Springer-Verlag Tokyo
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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