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Molecular mechanisms of RNAi-related factors involved in gene silencing

Research Project

Project/Area Number 16207011
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionThe University of Tokushima

Principal Investigator

SIOMI Mikiko  The University of Tokushima, Institute for Genome Research, Associate Professor, ゲノム機能研究センター, 助教授 (20322745)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2006: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2005: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2004: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
KeywordsRNAi / Argonaute / small RNA / RNA silencing / rasiRNA / germline / Drosophila / FMR1 / Slicer / 精神遅滞 / RNA / 翻訳抑制 / P-body / microRNAs / 脆弱X症候群 / 遺伝性精神遅滞 / 機能性RNA / 翻訳制御 / miRNA / Dicer
Research Abstract

Fragile X syndrome is the most common cause of inherited mental retardation. Since after the discovery of the responsible gene, fmr1, for Fragile X, we have been trying to understand molecular mechanisms underlying fmrl gene function. Through the study, we found that fly genome contains a single gene highly similar to human fmr1, dfmr1, and that the protein product of dfmrl, dFMR1, is able to associate with one of RNAi factors, Argonaute (AGO). To elucidate the function of dFMR1 (eventually human FMR1), we thought that it is necessary to elucidate the molecular mechanisms underlying RNAi and its related pathways, and identify protein factors necessary for the processes. Results we obtained for the three years supported by KIBAN(A) are summarized below ;
We found that AGO1 and AGO2 bind specifically with miRNA and siRNA, respectively, and function as Slicer in gene silencing mechanisms in Drosophila. We also showed that ATP is not required for RNAi reaction in vitro. It was also shown that AGO2 functions as siRNA duplex unwinder in RNAi (Miyoshi et al. Genes Dev 2005). Lately, we studied members of the Argonautes exclusively expressed in germline cells (AGO3, Aub, and Piwi) and found that they function in silencing selfish genes such as retrotransposons through associating with rasiRNAs originated from transcripts of retrotransposons in germlines (Saito et al. 2006, Gunawardane et al. 2007). We also proposed a 'rasiRNA biogenesis' model through our study (Gunawardane et al. 2007).

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (36 results)

All 2007 2006 2005 2004 Other

All Journal Article (23 results) Book (13 results)

  • [Journal Article] Expanding RNA physiology : microRNAs in a unicellular organism2007

    • Author(s)
      Haruhiko Siomi et al.
    • Journal Title

      Genes and Development (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A Slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila2007

    • Author(s)
      Gunawardane L.S. et al.
    • Journal Title

      Science 315

      Pages: 1587-1590

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Expanding RNA physiology : microRNAs in a unicellular organism2007

    • Author(s)
      Haruhiko Siomi et al.
    • Journal Title

      Genes & Dev (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome2006

    • Author(s)
      Kuniaki Saito et al.
    • Journal Title

      Genes and Development 20

      Pages: 2214-2222

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome2006

    • Author(s)
      Kuniaki Saito et al.
    • Journal Title

      Genes & Dev 20

      Pages: 2214-2222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Methods in Molecular Biology 3422006

    • Author(s)
      Akira Ishizuka et al.
    • Journal Title

      Humana press 10

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Slicer function of Drosophila Argonautes and its involvement in RISC formation2005

    • Author(s)
      Keita Miyoshi et al.
    • Journal Title

      Genes and Development 19

      Pages: 2837-2848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A potential link between transgene silencing and poly(A) tails.2005

    • Author(s)
      Mikiko C.Siomi et al.
    • Journal Title

      RNA 11

      Pages: 1004-1011

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.2005

    • Author(s)
      Kuniaki Saito et al.
    • Journal Title

      PLoS Biology 3(7)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Slicer function of Drosophila Argonautes and its involvement in RISC formation2005

    • Author(s)
      Keita Miyoshi et al.
    • Journal Title

      Genes & Dev 19

      Pages: 2837-2848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A potential link between transgene silencing and poly(A) tails2005

    • Author(s)
      Mikiko C.Siomi et al.
    • Journal Title

      RNA 11

      Pages: 1004-1011

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pediatric Nutrition in Chronic Diseases and Developmental Disorders-Prevention, Assessment, and Treatment-2nd Edition2005

    • Author(s)
      Nelson D et al.
    • Journal Title

      Oxford University Press 7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Methods in Molecular Biology 3092005

    • Author(s)
      Mikiko C.Siomi et al.
    • Journal Title

      Humana press 9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nuclear Import and Export in Plants and Animals2005

    • Author(s)
      Tetsuya Taura et al.
    • Journal Title

      Landes Bioscience 14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Slicer function of Drosophila Argonautes and its involvement in RISC formation.2005

    • Author(s)
      Keita Miyoshi et al.
    • Journal Title

      Genes & Development 19

      Pages: 2837-2848

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A potential link between transgene silencing and poly(A) tails2005

    • Author(s)
      Mikiko C.Siomi et al.
    • Journal Title

      RNA2005 11

      Pages: 1004-1011

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.2005

    • Author(s)
      Kuniaki Saito et al.
    • Journal Title

      Plos Biology 3・7

    • Related Report
      2005 Annual Research Report
  • [Journal Article] dentification of components of RNAi pathways using the Tandem Affinity Purification (TAP) method.2005

    • Author(s)
      Mikiko C.Siomi et al.
    • Journal Title

      Methods in Molecular Biology 2005 309

      Pages: 1-9

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Processing of pre-microRNAs by the Dicer-1-Locuacious complex Drosophila cells2005

    • Author(s)
      K.Saito
    • Journal Title

      PLoS Biology (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of components of RNA pathway using the Tandem Affinity Purification methods.2005

    • Author(s)
      M.C.Siomi
    • Journal Title

      Methods in Molecular Biology (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Distinct roles for Argonaute proteins in the small RNA gene silencing mechanism.2004

    • Author(s)
      K.Okamura
    • Journal Title

      Genes Dev. 18

      Pages: 1655-1666

    • Related Report
      2004 Annual Research Report
  • [Journal Article] RNA interference : a new mechanism by which FMRP acts on the normal brain? What can Drosophila teach us?2004

    • Author(s)
      H.Siomi
    • Journal Title

      MRDD Research Reviews 10

      Pages: 68-74

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysate.

    • Author(s)
      Akira Ishizuka et al.
    • Journal Title

      Methods in Molecular Biology (In press)

    • Related Report
      2005 Annual Research Report
  • [Book] Methods in Molecular Biology 3422006

    • Author(s)
      Akira Ishizuka et al.
    • Total Pages
      10
    • Publisher
      Humana Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] Pediatric Nutrition in Chronic Diseases and Developmental Disorders-Prevention, Assessment, and Treatment- 2nd Edition2005

    • Author(s)
      Nelson D et al.
    • Total Pages
      7
    • Publisher
      Oxford University Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Methods in Molecular Biology 3092005

    • Author(s)
      Mikiko C.Siomi et al.
    • Total Pages
      9
    • Publisher
      Humana Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Nuclear Import and Export in Plants and Animals2005

    • Author(s)
      Tetsuya Taura et al.
    • Total Pages
      14
    • Publisher
      Landes Bioscience
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] RNA工学の最前線2005

    • Author(s)
      三好啓太ら
    • Total Pages
      11
    • Publisher
      シーエムシー出版
    • Related Report
      2005 Annual Research Report
  • [Book] 化学と生物2005

    • Author(s)
      春原隆史ら
    • Total Pages
      7
    • Publisher
      学会出版センター
    • Related Report
      2005 Annual Research Report
  • [Book] Bio Clinica2005

    • Author(s)
      齋藤都暁ら
    • Total Pages
      6
    • Publisher
      北隆館
    • Related Report
      2005 Annual Research Report
  • [Book] Bio Clinica2005

    • Author(s)
      三好啓太ら
    • Total Pages
      7
    • Publisher
      北隆館
    • Related Report
      2005 Annual Research Report
  • [Book] 実験医学2005

    • Author(s)
      齋藤都暁ら
    • Total Pages
      1
    • Publisher
      羊土社
    • Related Report
      2005 Annual Research Report
  • [Book] RNAi法とアンチセンス法2005

    • Author(s)
      石塚明ら
    • Total Pages
      12
    • Publisher
      講談社
    • Related Report
      2005 Annual Research Report
  • [Book] RNAi法とアンチセンス法2005

    • Author(s)
      三好啓太ら
    • Total Pages
      10
    • Publisher
      講談社
    • Related Report
      2005 Annual Research Report
  • [Book] Pediatric Nutrition in Chronic Diseases and Developmental Disorders -Prevention, Assessment, and Treatment-2nd Edition.2005

    • Author(s)
      Nelson D. et al.
    • Total Pages
      3
    • Publisher
      Oxford university Press, New York.
    • Related Report
      2005 Annual Research Report
  • [Book] 実験医学増刊「躍進するRNA研究」small RNAが引き起こす遺伝子発現制御機構2004

    • Author(s)
      塩見美喜子
    • Total Pages
      11
    • Publisher
      羊土社
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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