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Studies on molecular mechanisms of reception and realization of Bombyx mori diapause hormone signal

Research Project

Project/Area Number 16208007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNagoya University

Principal Investigator

YAGINUMA Toshinobu  Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院生命農学研究科, 教授 (60135332)

Co-Investigator(Kenkyū-buntansha) NIIMI Teruyuki  Nagoya University, Graduate School of Bioagricutural Sciences, Assistant Professor, 大学院生命農学研究科, 助手 (00293712)
IKEDA Motoko  Nagoya University, Graduate School of Bioagricutural Sciences, Associate Professor, 大学院生命農学研究科, 助教授 (20262892)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥50,440,000 (Direct Cost: ¥38,800,000、Indirect Cost: ¥11,640,000)
Fiscal Year 2006: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2005: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2004: ¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
KeywordsBombyx mori embryonic diapause hormone / Quantification of diapause / Diapause hormone receptor / Transmembrane trehalase / Oxidation resistance gene 1 / Transformed silkworm / Embryonic RNAi / カイコ / 胚休眠 / 休眠ホルモン / トレハラーゼ / ドーパミン受容体 / 休眠誘導 / 休眠開始
Research Abstract

(1) Establishment of a sandwich ELISA system to detect diapause hormone
(2) Cloning and characterization of diapause hormone receptor
(3) Cloning and characterization of transmembrane trehalase
(4) Cloning and characterization of oxidation resistance gene 1
(5) Development of injection method into Bombyx mori eggs and vectors for production of transformed silkworms

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2006 2005

All Journal Article (21 results)

  • [Journal Article] G protein-coupled receptor for diapause hormone, an inducer of Bombyx embryonic diapause2006

    • Author(s)
      Homma, T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 344

      Pages: 386-393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Germ-line transformation and RNAi of the ladybird beetle, Harmonia axyridis2006

    • Author(s)
      Kuwayama, H.
    • Journal Title

      Insect Mol. Biol. 15

      Pages: 507-512

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diapause hormone-enhanced expression of the trehalase-2 gene encoding a novel membrane-penetrating type in Bombyx developing ovaries and the gene structure2006

    • Author(s)
      Mitsumasu, K.
    • Journal Title

      J. Insect Biotechnol. Sericol. 75

      Pages: 71-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Germ-line transformation and RNAi of the beetle, Harmonia axyridis2006

    • Author(s)
      Kuwayama, H.
    • Journal Title

      Insect Mol. Biol. 15

      Pages: 507-512

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The Pitx homoeobox gene in Bombyx mori : regulation of DH-PBAN neuropeptide hormone gene expression2006

    • Author(s)
      Shiomi, K.
    • Journal Title

      Moll. Cell. Neurosci.

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ナミテントウのlarvalRNAi法2006

    • Author(s)
      新美輝幸
    • Journal Title

      日本比較内分泌学会ニュース 121

      Pages: 32-37

    • NAID

      10016803650

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ナミテントウの遺伝子機能解析システムの現状と将来2006

    • Author(s)
      新美輝幸
    • Journal Title

      蚕糸・昆虫バイオテック 75

      Pages: 175-182

    • NAID

      10018486449

    • Related Report
      2006 Annual Research Report
  • [Journal Article] G protein-coupled receptor for diapause hormone, an inducer of Bombyx embryonic diapause2006

    • Author(s)
      Homma, T.
    • Journal Title

      Biochemical and biophysical Research Communications (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Diapause hormone-enhanced expression of the trehalase-2 gene encoding a novel membrane-penetrating type in Bombyx developing ovaries and the gene structure2006

    • Author(s)
      Mitsumasu, K.
    • Journal Title

      Journal of Insect Biotechnology and Sericology 75(2)(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Establishment of a sandwich ELISA system to detect diapause hormone, and developmental profile of hormone levels in egg and subesophageal ganglion of the silkworm, Bombyx mori2005

    • Author(s)
      Kitagawa, N.
    • Journal Title

      Zool. Sci. 22

      Pages: 213-221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diapause hormone levels in subesophageal ganglia of uni-, bi- and poly-voltine races during pupal-adult development of Bombyx mori, and the effects of ouabain, an inhibitor of Na^+-K^+ ATPase, on the hormone levels2005

    • Author(s)
      Kitagawa, N.
    • Journal Title

      J. Insect Biotechnol. Sericol 74

      Pages: 57-62

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Membrane-penetrating trehalase from Bombyx mori. Molecular cloning and localization in larval midgut2005

    • Author(s)
      Mitsumasu, K.
    • Journal Title

      Insect Mol. Biol. 14

      Pages: 501-508

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Larval RNAi applied to the analysis of postembryonic development in the ladybird beetle, Harmonia axyridis2005

    • Author(s)
      Niimi, T.
    • Journal Title

      J. Insect Biotechnol. Sericol. 74

      Pages: 95-102

    • NAID

      10016770272

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diapause hormone levels in subesophageal ganglia of uni-, bi-and poly-voltine races during pupal-adult development of Bombyx mori, and the effects of ouabain, an inhibitor of Na^+-K^+ ATPase, on the hormone levels2005

    • Author(s)
      Kitagawa, N.
    • Journal Title

      J. Insect Biotechnol. Sericol 74

      Pages: 57-62

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Membrane-penetrating trehalase from silkworm Bombyx mori. Molecular cloning and localization in larval midgut2005

    • Author(s)
      Mitsumasu, K.
    • Journal Title

      Insect Mol. Biol. 14

      Pages: 501-508

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] カイコの膜貫通型トレハラーゼ2005

    • Author(s)
      光増可奈子
    • Journal Title

      日本比較内分泌学会ニュース 171

      Pages: 19-21

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Membrane-penetrating trehalase from silkworm Bombyx mori : Molecular cloning and localization in larval midgut2005

    • Author(s)
      Mitsumasu, K.
    • Journal Title

      Insect Molecular Biology 14(5)

      Pages: 501-508

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Diapause hormone levels in subesophageal ganglia of uni-, bi-and poly-voltine races during pupal-adult development of Bombyx mori, and the effects of ouabain, an inhibitor of Na^+-K^+ ATPase, on the hormone levels.2005

    • Author(s)
      Kitagawa, N.
    • Journal Title

      Journal of Insect Biotechnology and Sericology 74(2)

      Pages: 57-62

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Establishment of a sandwich ELISA system to detect diapause hormone, and developmental profile of hormone levels in egg and subesophageal ganglion of the silkworm, Bombyx mori2005

    • Author(s)
      Kitagawa, N.
    • Journal Title

      Zool.Sci. 22(2)

      Pages: 213-221

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Diapause hormone levels in subesophageal ganglia of uni-, bi-, poly-voltine races during pupal-adult development of Bombyx mori, and the effects of ouabain, an inhibitor of Na+-K+ ATPase, on the hormone levels2005

    • Author(s)
      Kitagawa, N.
    • Journal Title

      J.Insect Biotechnol.Sericol. 74(2)(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Larval RNAi applied to the analysis of post embryonic development in the ladybird beetle, Harmonia axyridis2005

    • Author(s)
      Niimi, T.
    • Journal Title

      J.Insect Biotechnol.Sericol. 74(2)(In press)

    • NAID

      10016770272

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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