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Development of cultivation techniques of wild mushrooms based on compound culture system

Research Project

Project/Area Number 16208015
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学・森林工学
Research InstitutionShinshu University

Principal Investigator

YAMADA Akiyoshi  Shinshu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10324237)

Co-Investigator(Kenkyū-buntansha) KUGA Yukari  Shinshu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (30232747)
小倉 健夫  茨城県林業技術センター, きのこ特産部, 部長
増野 和彦  長野県林業総合センター, 特産部, 主任研究員
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2006: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2005: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2004: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
KeywordsEdible mushrooms / Forest management / Biotechnology / Mycorrhizal fungi / Saprotrophic fungi / artificial cultivation / Tricholoma matsutake / Symbiosis / ショウロ / クリタケ / アミガサタケ / チャナメツムタケ / 菌根性きのこの複合培養 / ミニリゾトロン / きのこ / 人工増殖 / 菌糸培養 / 菌根合成 / 子実体形成 / 菌床栽培 / 菌根苗
Research Abstract

1. We have clarified the unhealthy growth of mycorrhizal pine seedlings with Matsutake mushroom (Tricholoma matsutake) in vitro can be improved by the addition of dried yeast in the couture bottle.
2. We have clarified that Shouro mushroom (Rhizopogon rubesens) could be cultivated by direct inoculation of nursery pine seedlings with cultured mycelia that were prepared by newly established compound culture system.
3. We have clarified that direct transplantation of in vitro mycorrhizal pine seedlings to the nursery condition could be a practical technique in relation suitable culture strains of Amitake (Suillus bovinus), Shouro (R.rubesens), Shimofuri-shimeji (T.portentosum), Honshimeji (Lyophyllum shimeji) mushrooms.
4. We have succeeded in the fruiting of Shimofuri-shimeji, Mine-shimeji (T.sapoaceum), Kuma-shimeji (T.terreum), and Sumizome-shimeji (L.semitale) under open-pot culture condition in the tested 64 contoured strains belonging to 39 species in the genera such as Tricholoma, Sui … More llus, and Lactarius.
5. We have newly established cultured strains of Matsutake, Amigasatake (Morchella esculenta) and Chaname-tsumutake (Pholiota lubrica). In Matsutake it is clarified that cultured mycelium forms ectomycorrhizal structure in vitro with Pinus koraiensis, P. parviflora, and Picea abies.
6. We have clarified that Haru-shineji (Entoloma saepium) can form mycorrhizas with one-year old Prunus mune seedlings under natural field condition as well as an experimental condition where commercially produced P.mume seedlings were inoculated with basidiospres of E.saepium and maintained in the open pot culture, indicating that such mycorrhizal seedlings could be practically produced.
7. It was clarified by the minirhizotron observation that disturbed mycelial colony of matsutake does not recover rapidly and the disturbed areas are firstly colonized by other mycorrhizal fungal species.
8. We have clarified that Kuritake mushroom (Naematoloma sublateritium) forms rhizomrphs in soil, which could play an important role in their colony dispersal in soil. In addition, it was clarified by the microscopic observation that the rhizomorphs consist of its outer and inner layers. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (42 results)

All 2007 2006 2005 2004

All Journal Article (42 results)

  • [Journal Article] Sustainable fruit body formations of edible mycorrhizal Tricholoma species for three years in open pot culture with pine seedling hosts2007

    • Author(s)
      Yamada, A., Kobayashi, H., Ogura, T., Fukuda, M.
    • Journal Title

      Mycoscience 47(2)(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 大型培養容器によるマツタケのシロ様構造を有するマツ菌根苗の生産2007

    • Author(s)
      小林久泰, 小倉健夫, 倉持眞寿美, 小野瀬究明, 山田明義
    • Journal Title

      日本きのこ学会誌 15(印刷中)

    • NAID

      110008675305

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 食用菌根性きのこ類培養株の野外移植2007

    • Author(s)
      山田明義, 吉田 幸太, 佐藤 絵美子, 齋藤 千賀, 河村 史
    • Journal Title

      日本森林科学会学術講演集 118

    • NAID

      130005047431

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 管理施業の異なるアカマツ林における外生菌根バイオマスの比較2007

    • Author(s)
      岡田慶一, 山田明義
    • Journal Title

      日本森林科学会学術講演集 118

    • NAID

      130005047439

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Sustainable fruit body formation of edible mycorrhizal Tricholoma species for three years in open pot culture with pine seedling hosts2007

    • Author(s)
      Yamada, AA, Kobayashi, HH, Ogura, TT, Fukuda, M
    • Journal Title

      Mycoscience 47-2(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of Pine Seedlings with Shiro-like structure of Matsutake mushroom in a Large Culture Bottle2007

    • Author(s)
      Kobayashi, H, Ogura, T, Kuramochi M, Onos, K, Yamada, A
    • Journal Title

      Mushroom Science and Biotechnology 15(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Outplanting of cultured mycelia of edible mycorrhizal mushrooms2007

    • Author(s)
      Yamada, A, Yoshida, K, Satou, C, Kawamura F
    • Journal Title

      Proceedings of the Annual Meeting of the Japanese Forest Society 118

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison of ectomycorrhizal biomass among forests that are different in the forest management2007

    • Author(s)
      Okada, K, Yamada, A
    • Journal Title

      Proceedings of the Annual Meeting of the Japanese Forest Society 118

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sustainable fruit body formations of edible mycorrhizal Tricholoma species for three years in open pot culture with pine seeding hosts2007

    • Author(s)
      Yamada, A., Kobayashi, H., Ogura, T., Fukuda, M.
    • Journal Title

      Mycoscience 47 (2)(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 宿主植物実生の植え付けによる森林土壌からの外生菌根菌の検出2006

    • Author(s)
      山田明義, 山縣夏紀, 河村史紀, 岡田慶一, 細矢剛, 出川洋介, 福田正樹
    • Journal Title

      日本菌学会会報 47

      Pages: 25-32

    • NAID

      10018655100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Three-year analysis of ectomycorrhiza biomass in Pinus densiflora stands using different forest management regimes2006

    • Author(s)
      Okada, K., Yamada, A.
    • Journal Title

      International Conference on Mycorrhiza 5

      Pages: 163-163

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring 'shiro'2006

    • Author(s)
      Yamada, A., Maeda, K., Kobayashi, H., Murata, H.
    • Journal Title

      Mycorrhiza 16

      Pages: 111-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] マツタケ菌根苗作出における乾燥酵母添加の効果2006

    • Author(s)
      小林久泰, 小倉建夫, 山田明義
    • Journal Title

      日本森林科学会関東支部大会論文集 57

      Pages: 341-342

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Detection of ectomycorrhizal inocula from forest soils by the transplantation of host seedlings2006

    • Author(s)
      Yamada, A, Yamagata, N, Kawamura, F, Hosoya T, Degawa, Y
    • Journal Title

      Trans.Mycol.Soc.Jpn 47

      Pages: 25-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Three year analysis of ectomycorrhiza biomass in Pinus densiflora stands using different forest management regimes2006

    • Author(s)
      Okada, K, Yamada, A
    • Journal Title

      Proceedings of the International Conference on Mycorrhiza 5

      Pages: 163-163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densifiola seedlings that resembles naturally occurring 'shiro'2006

    • Author(s)
      Yamada, A, Maeda, K, Kobayashi, H, Murata, H
    • Journal Title

      Mycorrhiza 16

      Pages: 111-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of dry yeast on production of Japanese red pine seedling with mycorrhizas formed by Tricholoma matsutake2006

    • Author(s)
      Kobayashi, HH, Ogura, T, Yamada, A
    • Journal Title

      Proceedings of the Annual Meeting of the Kanto Branch of the Japanese Forest Society 57

      Pages: 341-342

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Three-year analysis of ectomycorrhiza biomass in Pinus densiflora stands using different forest management regimes2006

    • Author(s)
      Okada, K., Yamda, A.
    • Journal Title

      International Conference on Mycorrhiza 5

      Pages: 163-163

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring ‘shiro'2006

    • Author(s)
      Yamada A, Maeda K, Kobayashi H, Murata H
    • Journal Title

      Mycorrhiza 16

      Pages: 111-116

    • Related Report
      2005 Annual Research Report
  • [Journal Article] マツタケ菌根苗作出における乾燥酵母添加の効果2006

    • Author(s)
      小林久泰, 小倉建夫, 山田明義
    • Journal Title

      日本森林科学会関東支部大会論文集 57(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Production of mycorrhiazl seedlings with "Shiro" of Matsutake in a large culture bottle2005

    • Author(s)
      Kobayashi H, Ogura T, Yamada A
    • Journal Title

      Proceedings of the 4th International Workshop on Edible Mycorrhizal Mushrooms 4

      Pages: 76-76

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Mycorrhiza synthesis between Tricholoma matsutake and Pinaceae plants2005

    • Author(s)
      Yoshida K, Yamada A, Murata H, Kobayashi H
    • Journal Title

      MSA/MSJ Joint Meeting 2005 49

      Pages: 224-224

    • NAID

      130004614522

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Mycorrhizal morphology in Rosaceae and Ulmaceae produced by entolomatoid fungi in Japan2005

    • Author(s)
      Kobayashi H, Yamada A, Tokumasu S, Kakishima M
    • Journal Title

      MSA/MSJ Joint Meeting 2005 49

      Pages: 144-144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Assessment of mycorrhization ability in the genetically modified saprotrophic mushroom Lentinula edodes2005

    • Author(s)
      Kawamura F, Yamada A, Babasaki K
    • Journal Title

      MSA/MSJ Joint Meeting 2005 49

      Pages: 137-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Highly polymorphic DNA markers to specify strains of the ectomycorrhizal basidiomycete Tricholoma matsutake based on marY1, the long terminal repeat of gypsy-type retroelement marY12005

    • Author(s)
      Murata H, Babasaki K, Yamada A
    • Journal Title

      Mycorrhiza 15

      Pages: 179-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic mosaics in the massive persisting rhizosphere colony 'shiro' of the ectomycorrhizal basidiomycete Tricholoma matsutake.2005

    • Author(s)
      Murata H, Ohta A, Yamada A, Narimatsu M, Futamura N
    • Journal Title

      Mycorrhiza 15

      Pages: 505-512

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] レトロトランスポゾンの挿入部位に基づくマツタケの個体識別法2005

    • Author(s)
      村田仁, 馬場崎勝彦, 山田明義
    • Journal Title

      ブレインテクノニュース 107

      Pages: 35-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] マツタケの人工栽培に向けて-研究開発の現状と展望-2005

    • Author(s)
      山田明義
    • Journal Title

      Techno Innovation 55

      Pages: 24-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] マツタケとマツ科植物との菌根合成2005

    • Author(s)
      山田明義, 吉田幸太, 村田仁, 小林久泰
    • Journal Title

      日本森林学会大会講演要旨集 116

    • NAID

      130004614522

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Production of mycorrhiazl seedlings with 'shiro' of Matsutake in a large culture bottle2005

    • Author(s)
      Kobayashi, H, Ogura, T, Yamada, A
    • Journal Title

      Proceedings of the International Workshop on Edible Mycorrhiza Mushroom 4

      Pages: 76-76

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mycorrhiza synthesis between Tricholoma matsutake and Pinaceae plants2005

    • Author(s)
      Yoshida, K, Yamada, A, Kobayashi, H, Murata, H
    • Journal Title

      Proceedings of MSA/MSJ joint Meeting 49

      Pages: 224-224

    • NAID

      130004614522

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mycorrhizal morphology in Rosaceae and Ulmaceac produced by entolomatoid fungi in Japan2005

    • Author(s)
      Yamada, A, Kobayashi, H, Tokumsu, S, Kakishima, M
    • Journal Title

      Proceedings of MSA/MSJ joint Meeting 49

      Pages: 144-144

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Assessment of mycorrhization ability in the genetically modified saprotrophic mushroom Lentinula edodes2005

    • Author(s)
      Kawamura, F., Yamada, A., Babasaki, K.
    • Journal Title

      Proceedings of MSA/MSJ Joint Meeting 49

      Pages: 137-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Highly polymorphic DNA markers to specify strains of the ectomycorrhizal basidiomycete Tricholoma matsutake based on marY1, the long terminal repeat o gypsy-type retroelement marY12005

    • Author(s)
      Murata, H., Babasaki, K., Yamada, A.
    • Journal Title

      Mvcorrhiza 15

      Pages: 179-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic mosaics in the massive persisting rhizosphere colony `shiro' of the ectomycorrhizal basidiomycete Tricholoma matsutake.2005

    • Author(s)
      Murata H., Ohta A., Yamada A., Narimatsu M., Futamura N.
    • Journal Title

      Mvcorrhiza 15

      Pages: 505-512

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Clone discrimination of Tricholoma matsutake based on the insertion site of retrotransposon2005

    • Author(s)
      Murata, H., Babasaki, K., Yamada, A.
    • Journal Title

      BRAIN Techno News 107

      Pages: 35-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Artificial cultivation of Matsutake - Present situation a perspective -2005

    • Author(s)
      Yamada, A.
    • Journal Title

      Techno Innovation 55

      Pages: 24-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mycorrhizal synthesis between Tricholoma matsutake and Pinaceae plants2005

    • Author(s)
      Yamada, A., Yoshida, K., Murata, H., Kobayashi, H.
    • Journal Title

      Proceedings of the Annual Meeting of the Japanese Forest Society 116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Highly polymorphic DNA markers to specify strains of the ectomycorrhizal basidiomycete Tricholoma matsutake based on marY1, the long terminal repeat of gypsy-type retroelement marY12005

    • Author(s)
      Murata H, Babasaki K, Yamada A.
    • Journal Title

      Mycorrhiza (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic mosaics in the massive persisting rhizosphere colony 'shiro' of the ectomycorrhizal basidiomycete Tricholoma matsutake.2005

    • Author(s)
      Murata H, Ohta A, Yamada A, Narimatsu M, Futamura N
    • Journal Title

      Mycorrhiza (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 大型密閉容器中でのマツタケ菌根苗の成長における土壌水分量の影響2004

    • Author(s)
      小林久泰, 倉持眞寿美, 小倉健夫, 山田明義
    • Journal Title

      日本きのこ学会大会講演要旨集 8

      Pages: 73-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] The effects of soil water contents on the growth of Tricholoma matsutake colonized pine seedlings in the culture bottle.2004

    • Author(s)
      Kobayashi, H., Kuramochi, M., Ogura, T., Yamada, A.
    • Journal Title

      Proceedings of the Annual Meeting of the Japanese Mushroom Society 8

      Pages: 73-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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