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A novel regulatory way of smooth muscle contraction

Research Project

Project/Area Number 16209007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionGunma University

Principal Investigator

KOHAMA Kazuhiro  Gunma University, Department of Molecular and Cellular Pharmacology, Professor (30101116)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Ryoki  Gunma University, Department of Molecular and Cellular Pharmacology, Assoc. Prof. (20212863)
NAKAMURA Akio  Gunma University, Department of Molecular and Cellular Pharmacology, Assoc. Prof. (30282388)
YOSHIYAMA Shinji  Gunma University, Department of Molecular and Cellular Pharmacology, Assist Prof. (30396650)
TANAKA Hideyuki  Gunma University, Department of Faculty of Medicine, Research Associate (70343085)
NASU Ritsuko  Takasaki University, Department of Health & Welfare Nutritia, Assist Prof. (60383147)
登坂 雅彦  群馬大学, 大学院・医学系研究科, 助手 (40323357)
熊谷 啓之  群馬大学, 医学系研究科, 助手 (20321945)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2006: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2005: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2004: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
KeywordsActin / Myosin / smooth muscle / Regulation / migration / 薬理学 / 蛋白質 / 循環器・高血圧 / リン酸化 / ブレビスタチン / 血小板成長因子
Research Abstract

Myosin light-chain kinase (MLCK) of smooth muscle consists of an actin-binding domain at the N-terminal the catalytic domain in the central portion, and the myosin-binding domain at the C-terminal. The kinase activity is mediated by the catalytic domain that phosphorylates the myosin light-chain of 20 kDa (MLC20), activating smooth muscle myosin to interact with actin. Although the regulatory role of the kinase activity is well established, the role of non-kinase activity derived from actin-binding and myosin-binding domains remains unknown. We hypothesized that the actin-myosin interaction of smooth muscle could be activated by the non-kinase activity independently of MLC20 phosphorylation. We expressed cDNA of MLCK as a recombinant protein, and mutated it to lose the kinase activity, while keeping non-kinase activity unaffected. With the mutant, we detected an activating effect on the actin-myosin interaction. We also down-regulated MLCK expression by its RNAi in the cultured smooth muscle cells, to observe the lack of the activation. The rescue experiments with the MLCK-deficient cells demonstrated that MLC20 phosphorylation is not obligatory for the smooth muscle to contract.

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (48 results)

All 2008 2007 2006 2005 2004

All Journal Article (23 results) (of which Peer Reviewed: 8 results) Presentation (18 results) Book (2 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 1 results)

  • [Journal Article] Role of Non-kinase Activity of Myosin Light-chain Kinase in Regulating Smooth Muscle Contraction, a Review Dedicated to Dr. Setsuro Ebashi.2008

    • Author(s)
      Nakamura A., Xie C., Zhang Y., Gao Y., Wang H-H., Ye L-H., Kishi H., Okagaki T., Ybshiyama S., Hayakawa K., Ishikawa R., Kohama K.
    • Journal Title

      Biochem. Biophys. Res. Commun. 369

      Pages: 135-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Blebbistatin Inhibits the Chemotaxis of Vascular Smooth Muscle Cellsthrough Breaking the Interaction between Myosin II and Actin.2008

    • Author(s)
      Wang H., Tanaka H., Qin X., Zhao T., Ye L., Okagaki T., Katayama T., Nakamura A., Ishikawa R., Thatcher S., Wright G., and Kohama K.
    • Journal Title

      J. Physiol. Heart Circ.Physiol. 294

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Role of Non-kinase Activity of Myosin Light- chain Kinase in Regulating Smooth Muscle Contraction, a Review Dedicated to Dr. Setsuro Ebashi2008

    • Author(s)
      Nakamura A., Xie C., Zhang Y., Gao Y., Wang H-H., Ye L-H., Kishi H., Okagaki T., Yoshiyama S., Hayakawa K., Ishikawa R., Kohama K.
    • Journal Title

      Biochem. Biophys. Res. Commun 369

      Pages: 135-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Blebbistatin Inhibits the Chemotaxis of Vascular Smooth Muscle Cells through Breaking the Interaction between Myosin II and Actin2008

    • Author(s)
      Wang H., Tanaka H., Qin X., Zhao T., Ye L., Okagaki T., Katayama T., Nakamura A., Ishikawa R., Thatcher S., Wright G., and Kohama K.
    • Journal Title

      J. Physiol Heart Circ. Physiol 294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction,a review dedicated to Dr.Setsuro Ebashi2008

    • Author(s)
      Nakamura A., Xie C., Zhang Y., Gao Y., Wiang H-H., YeL-H., Kishi H., Okagalki T., Yoshiyama S., Hayakawa K., Ishikawa R., Kohama K.
    • Journal Title

      Biochem.Biophys.Res.Commun 369

      Pages: 135-143

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drebrin attenuates the interaction between actin and myosin-V2007

    • Author(s)
      Ishikawa R., Katoh K. Takaliashi A., Xie C., Oseki K., Watanabe M., Igarashi M., Nakamura A., Kohama K.
    • Journal Title

      Biochem.Biophys.Res.Commun 359

      Pages: 398-401

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Actin-binding Proteins in Nerve Cell Growth Cones2007

    • Author(s)
      Ishikawa R., Kohama K.
    • Journal Title

      J.Pharnacol.Sci 105

      Pages: 6-11

    • NAID

      10024315489

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular Signal Transduction For Migration and Actin Remodeling In Vascular Smooth Muscle Cells After Sphingosylphosphorylcholine Stimulation.2006

    • Author(s)
      Li S., Tanaka H., Wang H-H., Yoshiyama S., Kumagai H., Nakamura A., Brown D-L., Thatcher S-E., Wright G-L., and Kohama K.
    • Journal Title

      Am. J. Physiol. Heart Circ. Physiol. 291

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Blebbistatin Inhibits Sphingosylphosphorylcholine-Induced Contraction of Collagen-Gel Fiber Populated by Vascular Smooth-Muscle Cells.2006

    • Author(s)
      Katayama T., Yoshiyama S., Tanaka H., Wang H-H., Nakamura A., and Kohama K.
    • Journal Title

      J. Pharmacol. Sci. 102

      Pages: 339-342

    • NAID

      10020345252

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Intracellular Signal Transduction For Migration and Actin Remodeling In Vascular Smooth Muscle Cells After Sphingosylphosphorylcholine Stimulation2006

    • Author(s)
      Li S., Tanaka H., Wang H-H., Yoshiyama S., Kumagai H., Nakamura A., Brown D-L., Thatcher S-E., Wright G-L., and Kohama K.
    • Journal Title

      Am. J. Physiol. Heart Circ. Physiol 291

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Blebbistatin Inhibits Sphingosylphosphorylcholine-Induced Contraction of Collagen-Gel Fiber Populated by Vascular Smooth-Muscle Cells2006

    • Author(s)
      Katayama T., Yoshiyama S., Tanaka H., Wang H-H., Nakamura A., and Kohama K.
    • Journal Title

      J. Pharmacol. Sci 102

      Pages: 339-342

    • NAID

      10020345252

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Blebbistatin Inhibits Sphingosylphosphorylcholine-Induced Contraction of Collagen-Gel Fiber Populated by Vascular Smooth-Muscle Cells.2006

    • Author(s)
      Katayama, T., Yoshiyama, S., Tanaka, H., Wang, H., Nakamura, A., Kohama,K.
    • Journal Title

      J.Pharmacol. Sci. 102

      Pages: 339-42

    • NAID

      10020345252

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Intracellular signal transduction for migration and actin remodeling in vascular smooth muscle cells after sphingosylphosphorylcholine stimulation.2006

    • Author(s)
      Li, S., Tanaka, H., Wang, H-H., Yoshiyama, S., Kumagai, H., Nakamura, A., Brouwn, D-L., Thatceher, S-E., Wright, G-L., Kohama,K.
    • Journal Title

      Am. J. Physiol. (Heart & Circulatory Physiol.) 291

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A calmodulin-dependent protein kinase from lower eukaryote Physarum polycephamum2005

    • Author(s)
      Nakamura, A., Hanyuda, Y., Okagaki, T., Kohama, K.
    • Journal Title

      Biochem.Biophys.Res.Commun. 328

      Pages: 838-844

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Claps-specific binding of Two aminoacyl-tRNA synthetases to annexin, a Ca^<2+> and phospholipid-binding protein.2005

    • Author(s)
      Shirakawa T., Nakamura, A., Kohama, K., Hirakata, M., Ogihara, S.
    • Journal Title

      Cell Structure and Function 29

      Pages: 159-164

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Vanilloid receptor expressed in the sarcoplasmic reticulum of rat skeletal muscle.2005

    • Author(s)
      Xin, H., Tanaka H., Yamaguchi, M., Takemori S., Takamura, A., Kohama, k.
    • Journal Title

      Biochem.Biophys.Res.Commun. 332

      Pages: 756-762

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structual evidence for non-canonical binding of Ca^<2+> to a canonical EF-hand of a conventional Myosin.2005

    • Author(s)
      Debreczeni, J.E., Farkas, L., Harmat, V., Hetenyi, C., Hajdu, I., Zavodszky, P., Kohama, K., Nyitray, L.
    • Journal Title

      Journal of Biol.Chem. 50

      Pages: 41458-41464

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Vascular endothelium has a local anti-adenovirus vector system and Glucocorticoid optimizes its gene transduction.2005

    • Author(s)
      Murata, T., Hori, M., Lee, S., Nakamura, A., Kohama, K., Karaki, H., Ozaki, H.
    • Journal Title

      Arteroscler.Thromb.Vasc.Biol. 25

      Pages: 1896-1803

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A calmoduin-dependent protein kinase from lower eukaryote Physarum polycephalum.2005

    • Author(s)
      Nakamura, A., Hanyuda, Y., Okagaki, T., Takagi, T., Kohama, K.
    • Journal Title

      Biochem.Biophys.Res Commun. 328

      Pages: 838-844

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nicotinic Acetylcholine receptor α7 Subunit Mediates Migration of Vascular Smooth Muscle Cells Toward Nicotine.2004

    • Author(s)
      Li S., Zhao T., Xin H., Ye LH., Zhang X., Tanaka H., Nakamura A., and Kohama K.
    • Journal Title

      J. Pharmacol, Sci. 94

      Pages: 334-338

    • NAID

      130000073854

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nicotinic Acetylcholine receptor a7 Subunit Mediates Migration of Vascular Smooth Muscle Cells Toward Nicotine2004

    • Author(s)
      Li S., Zhao T., Xin H., Ye LH., Zhang X., Tanaka H., Nakamura A., and Kohama K.
    • Journal Title

      J. Pharmacol. Sci 94

      Pages: 334-338

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nicotinic acetylcholine receptor alpha7 subunit mediates migration of vascular smooth muscle cells toward nicotine.2004

    • Author(s)
      Li, S, Zhao, T, Xin, H, Ye, LH, Zhang, X, Tanaka, H, Nakamura, A, Kohama, K.
    • Journal Title

      J.Pharmacol.Sci. 94

      Pages: 334-338

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Protein phosphatase 2B dephosphorylates twitchin, initiating the catch state of invertebrate smooth muscle2004

    • Author(s)
      Yamada, A., Yoshio, M., Nakamura, A., Kohama, K., Oiwa, K.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 40762-40768

    • Related Report
      2004 Annual Research Report
  • [Presentation] Blebbistatin inhibits the chemotaxis of vascular smooth muscle cells through breaking the interaction between smooth muscle myosin II and actin.2008

    • Author(s)
      Wang, H-H., Tanaka, H., Qin, X., Zhao, T., Lihong, Ye, Okagaki, T., Katayama, T., Nakamura, A., Ishikawa, R., Thatcher, S. E., Wright G. L., and Kohama, K.
    • Organizer
      The Joint Biophysical Society 52nd Annual Meeting and 16th International Biophysics Congress
    • Place of Presentation
      California
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Blebbistatin inhibits the chemotaxis of vascular smooth muscle cells through breaking the interaction between smooth muscle myosin II and actin2008

    • Author(s)
      Wang H-H., Tanaka H., Qin X., Zhao T., Lihong Ye, Okagaki T., Katayama T., Nakamura A., Ishikawa R., Thatcher S.E., Wright G. L., and Kohama K.
    • Organizer
      The Joint Biophysical Society 52nd Annual Meeting and 16th International Biophysics Congress
    • Place of Presentation
      California
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The mn-kinase actiVity of myosin light chain kinase in regulating smooth musc1e contraction2008

    • Author(s)
      Nakamura A.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Screening of phenotype-specific genes of vascular smooth muscle cell(VSMC) and their characterization2008

    • Author(s)
      Miyamo H., Nagahama R, Nakamura A., Kohama K., Okagaki T.
    • Organizer
      The 81st Arnual Meeting of The Japanese Pharrnacological-Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brain specific actin-binding protein Drebrin inhibits the cofilin-dependent actin dynamics2008

    • Author(s)
      Ishikawa R., Katoh K., Ebihara S., Takahashi A., Igarashi M., Nakamura A., Kohama K.
    • Organizer
      The 81th Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Utrsstructural change of myosin filaments in smooth muscle cell with blebbistain2008

    • Author(s)
      Tanaka-H., Watanabe M., Wang H-H., Kohama K.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Arachidonic acid binds to heavy chain of smooth muscle myosin and stimulates ATPase activity2008

    • Author(s)
      Katayama T., Miyakawa T., Yoshiyama S., Makamura A., Tanaka H., Tanokura M., Kohama K.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Non-kinase acticity of MLCK in elongated fiopodia formation and chemotaxis of vasculaar smooth muscle cells in response to sphingosylphosphorylcholine2008

    • Author(s)
      Wang H-H., Nakamura A., Mastumoto A., Hayakawa K., Yoshiyama S., Qin X., Tanaka H., Ye L., Li S., Ishikawa R., Kohama K.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacolosical Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Effect of a high-fat diet on diabetic mother rats and their offsprings and insulin signaling2008

    • Author(s)
      Nasu R., Kohama T., Kohama K., Kakarla S., Blough E.R.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Dynamics of actomyosin and intracellular Ca^<2+> during cell movement, in case of Physarum polycephalm2008

    • Author(s)
      Yoshiyama S., Ishigami M., Kohama K.
    • Organizer
      The 81st Annual Meeting if The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression and characterization of the kinase-dend ,utants of myosin light chain kinase2008

    • Author(s)
      Xie C., Nakamura A., Zhang Y., Gao Y., Kawano K., Ishikawa R., Matsumoto A., Tanaka H., Kohama K.
    • Organizer
      The 82st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] The regulatory effect of Ca^<2+>/calmodulin on the actin-binding activity of smooth muscle chain kinase2008

    • Author(s)
      Zhang Y., Xie C., Nakamura A., Gao Y., Ishikawa R., Yoshiyama S., Mastumoto A., Kohama k.
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Related Report
      2007 Annual Research Report
  • [Presentation] Dr Ebashi's contributions to smooth muscle research.2007

    • Author(s)
      Kohama K
    • Organizer
      5th United States-Japan Workshop on Cellular and Molecular Aspects of Vascular Smooth Muscle Function, Jan. 7-9, 2007, Hawaii
    • Place of Presentation
      Hawaii
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Dr Ebashi's contributions to smooth muscle research2007

    • Author(s)
      Kohama K.
    • Organizer
      5th United States-Japan Workshop on Cellular and Molecular Aspects of Vascular Smooth Muscle Function
    • Place of Presentation
      Hawaii
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phosphorylation of Recombinant Smooth Muscle Myosin Light Chain Kinase by Various Ser/Thr Kinase.2006

    • Author(s)
      Nakamura A., Ishikawa R., Kohama K.
    • Organizer
      The American Society for Cell Biology, 2239, B143, 46th Annual Meeting
    • Place of Presentation
      San Diego
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phosphorylation of Recombinant Smooth Muscle Myosin Light Chain Kinase by Various Ser/Thr Kinase2006

    • Author(s)
      Nakamura A., Ishikawa R., Kohama K.
    • Organizer
      The American Society for Cell Biology, 2239, B143, 46th Annual Meeting
    • Place of Presentation
      San Diego
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Full-length Smooth Muscle Myosin Light Chain Kinase.2005

    • Author(s)
      Nakamura A., Kawano K., Gao Y., Hanyuda Y., Takahashi A., Ishikawa R., Kohama K.
    • Organizer
      The American Society for Cell Biology, 916, B13945th Annual Meeting
    • Place of Presentation
      San Francisco
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Full-length Smooth Muscle Myosin Light Chain Kinase2005

    • Author(s)
      Nakamura A., Kawano K., Gao Y., Hanyuda Y, Takahashi A., Ishikawa R., Kohama K.
    • Organizer
      The American Society for Cell Biology, 916 B139 45th Annual Meeting
    • Place of Presentation
      San Francisco
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] REGURATORY MECHANISMS OF STRIATED MUSCLE CONTRACTION2006

    • Author(s)
      Kawamichi H., Zhang Y., Hino M., Nakamura A., Farkas L., Nyitray L., and Kohama K.
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Regulatory Mechanisms of Striated Muscle Contraction.2006

    • Author(s)
      Kawamichi, H., Zhang, Y., Hino, M., Nakamura, A., Farkas, L., Nyitray, L., Kohama,K.
    • Publisher
      Springer
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 束化アクチンを用いたマイクロアクチュエーター2006

    • Inventor(s)
      小浜一弘 他6名
    • Industrial Property Rights Holder
      国立大学法人群馬大学他1名
    • Acquisition Date
      2006-02-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 生体適合性ブロック共重合体、その用途及び製造法2006

    • Inventor(s)
      小濱一弘, 片貝良一, 鈴木嘉樹
    • Industrial Property Rights Holder
      小濱一弘, 片貝良一, 鈴木嘉樹
    • Filing Date
      2006-10-02
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体適合性ブロック共重合体、その用途及び製造方法2005

    • Inventor(s)
      小濱 他
    • Industrial Property Rights Holder
      小濱 他
    • Filing Date
      2005-10-04
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 束化アクチンを用いたマイクロアクチュエータ2005

    • Inventor(s)
      日野 瑞城, 小濱 一弘, 他5名
    • Industrial Property Number
      2005-038287
    • Filing Date
      2005-02-15
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 平滑筋弛緩剤とその有効成分の抽出法2004

    • Inventor(s)
      小浜一弘 他4名
    • Industrial Property Rights Holder
      国立大学法人群馬大学他1名
    • Industrial Property Number
      2004-108238
    • Filing Date
      2004-05-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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