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Development of a high sensitivity analytical system for breath and its clinical use

Research Project

Project/Area Number 16209020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

SHIMOUCHI Akito  National Cardiovascular Center Research Institute, National Cardiovascular Center Research Institute, Department of Etiology and Pathogenesis, Laboratory Chief (80211291)

Co-Investigator(Kenkyū-buntansha) KONDO Takaharu  Nagoya University, Research Center of Health, Physical Fitness and Sports, Department of Health and Nutrition, Professor (20135388)
蓮見 啓二  (株)ルネサス東日本セミコンダクタ日立中央研究所, 分室長(研究職)
植田 秀雄  (株)タイヨウミトレーベン研究所, 代表(研究職)
野田 恵一  (株)日本エイピーアイ分析技術部, 主任研究員
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥46,410,000 (Direct Cost: ¥35,700,000、Indirect Cost: ¥10,710,000)
Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2006: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2005: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2004: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
KeywordsBreath / Mass spectrography / Non-invasive examination / Skin gas / high sensitivity analysis / 高感度 / 生活習慣病 / 呼気採取 / 高感度質量分析 / 高感度分析 / 大気圧イオン化質量分析
Research Abstract

In this project, we developed a high sensitivity analytical system for human breath and applied it to clinical use. Chemical compounds in breath originate mainly from volatile metabolic products in health and diseases. Among them, hydrogen and methane are believed to represent the conditions of gastro-intestinal systems in the presence of bacterial flora. On the other hands, carbon monoxide and nitric oxide are well-known bioactive chemicals to play important roles in physiological and pathophysiological conditions. Fasted 1011 subjects aged from 20 to 88 years old volunteered for the study. After tooth brushing and gargling, fasted subjects breathed purified artificial air via a mouthpiece in a sitting position for 12 min, during which exhaled air was collected via one-way valve into the Douglas bag. Minute ventilation volumes of carbon dioxide(CO2), oxygen(O2), hydrogen(H2), methane(CH4), carbon monoxide(CO), nitric oxide(NO) and nitrogen oxide(NOX) were measuredby the standardmethod … More s. All data were normalized by body surface area. First, V02, VCO2 and VH2 were significantly decreased in age-dependent manner. Contrarily, VCH4 was increased in aged subjects. VCO was significantly increased to aging especially in women, while VNO did not significantly changed. Significant increases in VCO and decrease in VNO were observed in heavy smokers. VCO was increased in the 4th weeks after the starting date of menstruation. Age, sex, smoking habits and menstruation cycle must be concerned for the evaluation of low chemical compounds in breath. In addition to the above results, we found that (1) Dietary turmeric activated bowel motility and colonic fermentation, (2) Ethanol and acetaldehyde were detected in the skin gas after ingestion of alcohol. Their peaks appeared 30 min after those in breath and venous blood, (3) Carbon monoxide and nitrogen oxide in breath and skin gas could be reversibly and acutely altered during 2-day smoking cessation and restart even in case of a long-term cigarette smoker. Less

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (34 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (20 results) (of which Peer Reviewed: 5 results) Presentation (8 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Changes in concentrations of carbon monoxide and nitric oxide in breath and skin gas during smoking cessation and restart2008

    • Author(s)
      Nose K, Shimouchi A.
    • Journal Title

      J Breath Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Changes in the mass spectrums of breath and skin gas during exercise2008

    • Author(s)
      Shimouchi A, Nose K.
    • Journal Title

      J Breath Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Delayed appearance of ethanol and acetaldehyde in the skin gas after alcohol ingestion.2008

    • Author(s)
      Shimouchi A, Nose K, Okina M, Kano K, Wado H, Takeuchi.
    • Journal Title

      J Breath Research. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Changes in the mass spectra of breath and skin gas during exercise.2008

    • Author(s)
      Shimouchi A, Nose K.
    • Journal Title

      J Breath Research. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Changes in concentrations of carbon monoxide and nitric oxide in breath and skin gas during smoking cessation and restart.2008

    • Author(s)
      Nose K, Shimouchi A.
    • Journal Title

      J Breath Research. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Changes in concentrations of carbon monoxide and nitric oxide in breath and skin gas during smoking cessation and restart2008

    • Author(s)
      Nose K, Shimouchi A.
    • Journal Title

      J Breath Research (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in the mass spectrums of breath and skin gas during exercise2008

    • Author(s)
      Shimouchi A, Nose K.
    • Journal Title

      J Breath Research (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] alpha(2)-Adrenoreceptor mediated sympathoinhibition of heart rate during acute hypoxia is diminished in conscious prostacyclin synthase deficient mice2007

    • Author(s)
      Pearson JT, Shirai M, Shimouchi A, et. al.
    • Journal Title

      Pflugers Arch. 454

      Pages: 29-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] alpha(2)-Adrenoreceptor mediated sympathoinhibition of heart rate during acute hypoxia is diminished in conscious prostacyclin synthase deficient mice.2007

    • Author(s)
      Pearson JT, Shirai M, Shimouchi A, et. al.
    • Journal Title

      Pflugers Arch. 454

      Pages: 29-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Permeation of volatile chemical compounds through an egg shell as determined by a newly developed high-sensitivity analytical system2007

    • Author(s)
      Shimouchi A, Chiba Y, Pearson JT et al.
    • Journal Title

      The world's Poultry Science : New insights into fundamental physiology and peri-natal adaptation of domestic fowl

      Pages: 181-188

    • Related Report
      2006 Annual Research Report
  • [Journal Article] alpha(2)-Adrenoreceptor mediated sympathoinhibition of heart rate during acute hypoxia is diminished in conscious prostacyclin synthase deficient mice.2007

    • Author(s)
      Pearson JT, Shirai M, Shimouchi A, et al.
    • Journal Title

      Pflugers Arch 454(1)

      Pages: 29-39

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 高感度生体ガス分析による非侵襲的診断法の開発に向けたモデル実験2007

    • Author(s)
      下内章人
    • Journal Title

      循環器病研究の進歩 27

      Pages: 41-48

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Permeation of volatile chemical compounds through an eggshell as determined by a newly developed high-sensitivity analytical system.2006

    • Author(s)
      Shimouchi A, Chiba Y, Pearson JT, Shirai M, Tazawa H.
    • Journal Title

      "New insights into fundamental physiology and peri-natal adaptation of domestic fowl" edited by Yahav S, Tschentke B, Nottingham University Press, United kingdom

      Pages: 181-188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 喫煙者における呼気・皮膚ガス成分の特徴に関する検討2006

    • Author(s)
      下内章人, 野瀬和利
    • Journal Title

      呼気病態生化学の進歩 9

      Pages: 3-7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 禁煙・喫煙に伴う呼気及び皮膚ガス成分の動態2006

    • Author(s)
      野瀬和利, 下内章人
    • Journal Title

      呼気病態生化学の進歩 9

      Pages: 9-12

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Chemical permeation through an eggshell as determined by a newly developed high- sensitivity analytical system2006

    • Author(s)
      Shimouchi A, Chiba Y, Pearson JT, et al.
    • Journal Title

      World Poultry Science Journal 61(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 高感度質量分析による皮膚ガスと呼気中の微量ガス成分の対比2005

    • Author(s)
      下内章人, 藤川和子, 瀬戸純子
    • Journal Title

      呼気生化学の進歩 7

      Pages: 45-46

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 種々のガス採取容器の低分子ガス保存能力に関する検討2005

    • Author(s)
      藤川和子, 下内章人, 瀬戸純子
    • Journal Title

      呼気生化学の進歩 7

      Pages: 47-52

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cardiovascular hemodynamic responses to chronic intermittent hypoxia in free-moving rats2004

    • Author(s)
      Shimouchi A, Ukai K, Chiba Y, et al.
    • Journal Title

      American Journal of respiratory and Critical care Medicine 167(7)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comaparative studies on care-giving for the patients with chronic respiratory failure or sequelae of cerebral strokes2004

    • Author(s)
      Morimoto T, Shimouchi A, Asano H
    • Journal Title

      American Journal of respiratory and Critical care Medicine 167(7)

    • Related Report
      2004 Annual Research Report
  • [Presentation] Breath hydrogen is increased by ingestion of turmeric, as a possible role of antioxidant?2008

    • Author(s)
      Akito Shimouchi, et. al.
    • Organizer
      HSSRC/AIST-NIEHS/NIH Joint International Symposium: Biomarkers of Oxidative Stress in Health and Diseases
    • Place of Presentation
      大阪府豊中市
    • Year and Date
      2008-01-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Breath hydrogen is increased by ingestion of turmeric, as a possible role of antioxidant?2008

    • Author(s)
      Shimouchi A, et. al.
    • Organizer
      HSSRC/AIST-NIEHS/NIH Joint International Symposium : Biomarkers of Oxidative Stress in Health and Diseases
    • Place of Presentation
      Toyonaka, Japan
    • Year and Date
      2008-01-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Breath hydrogen is increased by ingestion of turmeric, as a possible role of antioxidant?2008

    • Author(s)
      Akito Shimouchi, et. al.
    • Organizer
      HSSRC/AIST-NIEHS/NIH Joint International Symposium : Biomarkers of Oxidative Stress in Health and Diseases
    • Place of Presentation
      大阪府豊中市
    • Year and Date
      2008-01-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Changes in the mass spectrums of breath and skin gas during exercise2007

    • Author(s)
      Akito Shimouchi, Kazutoshi Nose
    • Organizer
      Breath Analysis Summit 2007
    • Place of Presentation
      クリーブランド
    • Year and Date
      2007-11-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Changes in the mass spectrums of breath and skin gas during exercise2007

    • Author(s)
      Shimouchi A, Nose K.
    • Organizer
      Breath Analysis Summit 2007
    • Place of Presentation
      Cleaveland
    • Year and Date
      2007-11-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Minute ventilation volumes of low molecular weight compounds in breath2007

    • Author(s)
      Shimouchi A, Nose K
    • Organizer
      American Thoracic Society
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2007-05-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Minute ventilation volumes of low molecular weight compounds in breath2007

    • Author(s)
      Shimouchi A, Nose K.
    • Organizer
      American Thoracic Society
    • Place of Presentation
      San, Francisco, USA
    • Year and Date
      2007-05-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular dependency of permeation rates through an avian eggshell observed by a newly developed high-sensitivity analytical system2006

    • Author(s)
      Shimouchi A, et. al.
    • Organizer
      American Thoracic Society
    • Place of Presentation
      Diego, USA
    • Year and Date
      2006-05-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 酸素と体の事典 「低酸素性肺血管攣縮」(分担執筆)2008

    • Author(s)
      下内章人, 白井幹康
    • Publisher
      朝倉書店(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 酸素と体の事典 「質量分析装置」(分担執筆)2008

    • Author(s)
      野瀬和利, 下内章人
    • Publisher
      朝倉書店(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 酸素と体の事典 「低酸素性肺血管攣縮」 (分担執筆)2008

    • Author(s)
      下内章人,白井幹康
    • Publisher
      朝倉書店(印刷中)
    • Related Report
      2007 Annual Research Report
  • [Book] 酸素と体の事典 「質量分析装置」 (分担執筆)2008

    • Author(s)
      野瀬和利,下内章人
    • Publisher
      朝倉書店(印刷中)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncvc.go.jp/res/resj.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.ncvc.go.jp/res/resj.html

    • Related Report
      2007 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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