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Novel cholineacetyltransferase variants (pChAT) in the brain of human and monkey

Research Project

Project/Area Number 16300111
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionShiga University of Medical Science

Principal Investigator

TOOYAMA Ikuo  Shiga University of Medical Science, Molecular Neuroscience Research Center, Professor, 分子神経科学研究センター, 教授 (20207533)

Co-Investigator(Kenkyū-buntansha) KIMURA Hiroshi  Shiga University of Medical Science, Molecular Neuroscience Research Center, Professor, 分子神経科学研究センター, 教授 (40079736)
MATSUO Akinori  Shiga University of Medical Science, Molecular Neuroscience Research Center, Research Assosiate, 分子神経科学研究センター, 助手 (20324585)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsacetylcholine / cholinergic neurons / alternative splicing / antibody / learning / memory / alternative splicin
Research Abstract

Although most ChAT antibodies clearly stain central cholinergic neurons, it is difficult to reveal peripheral cholinergic system. The difficulty suggests that different forms of ChAT may exist in peripheral tissues. To test the hypothesis, we investigated the rat pterygopalatine ganglion, which is a structure that contains many cholinergic neurons sending their axons peripherally to cerebral vessels. From the experiments, we found a splice variant of ChAT mRNA expressed, not in the brain, but in the pterygopalatine. According to the genomic structure of ChAT, the variant lacks exons 6, 7, 8 and 9. The cDNA sequence has no frame shift and preserves regions of the ChAT protein which are essential for its catalytic function. Since the variant is expressed preferentially in peripheral tissues, we termed the form as pChAT(peripheral type of ChAT). And we termed the known ChAT as cChAT (common type of ChAT).
In this study, we investigated the structure of cChAT ad pChAT of human and monkey. We cloned cDNAs of cChAT and pChAT from the placenta of human and monkey. In the second step of our experiments, we produced antisera specific to cChAT and pChAT. Using these antisera, we investigated localizations of cChAT and pChAT proteins in the brain of rat, monkey and human. We have published the results in eleven papers of international journals (List in the next page). Findings of pChAT should facilitate investigations of cholinergic mechanism in the central and peripheral nervous system.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (31 results)

All 2007 2006 2005 2004

All Journal Article (30 results) Book (1 results)

  • [Journal Article] The production of antibodies that distinguish rat choline acetyltransferase from its splice variant product of a peripheral type.2007

    • Author(s)
      Kimura S, Bellier J-P, Matsuo A, Tooyama I, Kimura H
    • Journal Title

      Neurochemical International 581

      Pages: 251-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Immunohistochemical mapping of NPR-A in the brainstem of Macaca Fascicularis.2007

    • Author(s)
      Abdelalim EM, Osman, AHK, Takada T, Torii R, Tooyama I
    • Journal Title

      Neuroscience 145

      Pages: 1087-1096

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The production of antibodies that distinguish rat choline acetyltransferase from its splice variant product of a peripheral type.2007

    • Author(s)
      Kimura S, Bellier J-P, Matsuo A, Tooyama I, Kimura H
    • Journal Title

      Neurochem Internatinal 50

      Pages: 251-255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Immunohistochemical mapping of natriuretic peptide receptor-A in the brainstem of Macaca fascicularis.2007

    • Author(s)
      Abdelalim EM, Osman AH, Takada T, Torii, Tooyama I
    • Journal Title

      Neuroscience 145

      Pages: 1087-1096

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of FGF1 (aFGF) Expression between the Dorsal Motor Nucleus of Vagus and the Hypoglossal Nucleus of Rat.2006

    • Author(s)
      Toyoda K, Okano H, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Tooyvma I
    • Journal Title

      Acta Histochemica et Cytochemica 39

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular Cloning of BNP from Heart and Its Immunohistochemical Localization in the Hypothalamus of Monkey.2006

    • Author(s)
      Abdelalim EM, Takada T, Torii R, Tooyama I
    • Journal Title

      Peptides 29

      Pages: 1886-1893

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Localization of fibroblast growth factor-1 (FGF1) in cholinergic neurons innervating the rat larynx.2006

    • Author(s)
      Okano H, Toyoda K, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Kimura H, Tooyama I
    • Journal Title

      Journal of Histochemistry and Cytochemistry 54

      Pages: 1061-1067

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison of FGF1(aFGF) Expression between the Dorsal Motor Nucleus of Vagus and the Hypoglossal Nucleus of Rat.2006

    • Author(s)
      Toyoda K, Okano H, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Tooyama I
    • Journal Title

      Acta Histochem Cytochem 39

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular Cloning of BNP from Heart and Its Immunohistochemical Localization in the Hypothalamus of Monkey.2006

    • Author(s)
      Abdelalim EM, Takada T, Torii R, Tooyama I
    • Journal Title

      Peptides 27

      Pages: 1886-1893

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Localization of fibroblast growth factor-1(FGF1) in cholinergic neurons innervating the rat larynx.2006

    • Author(s)
      Okano H, Toyoda K, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Kimura H, Tooyama I
    • Journal Title

      J Histochem Cytochem 54

      Pages: 1061-1067

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Localization of fibroblast growth factor-1 (FGF1) in cholinergic neurons innervating the rat larynx.2006

    • Author(s)
      Okano H, Toyoda K, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Kimura H, Tooyama I
    • Journal Title

      J Histochem Cytochem 54

      Pages: 1061-1067

    • Related Report
      2006 Annual Research Report
  • [Journal Article] In vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells.2006

    • Author(s)
      Abdelalim EM, Takada T, Toyoda F, Omatsu-Kanbe M, Matsuura H, Tooyama I, R Torii.
    • Journal Title

      Biochem Biophys Res Commun 340

      Pages: 689-695

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparison of FGF1 (aFGF) Expression between the Dorsal Motor Nucleus of Vagus and the Hypoglossal Nucleus of Rat2006

    • Author(s)
      Toyoda K, Okano H, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Tooyama I
    • Journal Title

      Acta Histochem Cytochem 39

      Pages: 1-7

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Magnetic resonance imaging using hemagglutinating virus of Japan-envelope vector successfully detects localization of intra-cardially administered microglia in normal mouse brain2006

    • Author(s)
      Song Y, Morikawa S, Morita M, Inubushi T, Takada T, Torii R, Toyama I
    • Journal Title

      Neurosci Lett 395

      Pages: 42-45

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Rat choline acetyltransferase of the peripheral type differs from that of the common type in untracellular translocation.2005

    • Author(s)
      A Matsuo, JP Bellier, T Hisano, Y Aimi, O Yasuhara, I Tooyama, N Saito, H Kimura
    • Journal Title

      Neurochemical International 46

      Pages: 423-433

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Immunohistochemical study of TAFII250 in the rat laryngeal nervous system.2005

    • Author(s)
      Okano H, Bamba H, Hisa Y, Makino S, Ando S, Tammiya G, Goto S, Kaji R, Tooyama I
    • Journal Title

      Histology and Histopathology 20

      Pages: 1049-1035

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells.2005

    • Author(s)
      Abdelalim EM, Takada T, Toyoda F, Omatsu-Kanbe M, Matsuura H, Tooyama I, Torii R
    • Journal Title

      Biochem Biophys Res Commun 340

      Pages: 689-695

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rat choline acetyltransferase of the peripheral type differs from that of the common type in untracellular translocation.2005

    • Author(s)
      A Matsuo, JP Bellier, T Hisano, Y Aimi, O Yasuhara, I Tooyama, N Saito, H Kimura
    • Journal Title

      Neurochem International 46

      Pages: 423-433

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Immunohistochemical study of TAFII250 in the rat laryngeal nervous system.2005

    • Author(s)
      Okano H, Bamba H, Hisa Y, Makino S, Ando S, Tammiya G, Goto S, Kaji R, Tooyama I
    • Journal Title

      Histochem Histopathol 20

      Pages: 1049-1035

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cloning and structure of monkey cChAT from the striatum of Japanese macaque (Macaca fuscata)2005

    • Author(s)
      Song Y, Kimura S, Bellier JP, Yasuhara O, Kimura H, Torii, R, Tooyama I
    • Journal Title

      Neuroscience Research 52(Supple)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunohistochemical study of TAFII250 in the rat laryngeal nervous system2005

    • Author(s)
      Okano H, Bamba H, Hisa Y, Makino S, Ando S, Tammiya G, Goto S, Kaji R, Tooyama I
    • Journal Title

      Histol Histopathol 20

      Pages: 1029-1035

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Rat cholineacetyltransferase of the peripheral type differs from that of the common type in intracellular translocation.2005

    • Author(s)
      A Matsuo, JP Belier, T Hisano, Y Aimi, O Yasuhara, I Tooyama, N Saito, H Kimura
    • Journal Title

      Neurochemistry International 46・5

      Pages: 423-433

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Coexpression pattern of two variants of choline acetyltransferase and neuronal nitric oxide synthase in myenteric neurons of porcine ileum.2004

    • Author(s)
      A Brehmer, F Schrodl, W Neuuber, I Tooyama, H Kimura
    • Journal Title

      Journal of Chemical Neuroanatomy 27

      Pages: 33-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Innervation of rat iris by trigeminal and ciliary neurons expressing pChAT, a novel splice variant of choline acetyltranferase.2004

    • Author(s)
      O Yasuhara, Y Aimi, A Shibano, A Matsuo, JP Bellier, M Park, I Tooyama, H Kimura
    • Journal Title

      Journal Comparative Neurology 472

      Pages: 232-245

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fate of (D-Ala2)-deltorphin-I-like immunoreactive neurons in 6-hydroxydopamine lesioned rat brain.2004

    • Author(s)
      A Casini, A Pinna, I Tooyama, H Kimura, G Di Chiara, TG Renda
    • Journal Title

      European Journal of HIstochemistry 48

      Pages: 135-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coexpression pattern of two variants of choline acetyltransferase and neuronal nitric oxide synthase in myenteric neurons of porcine ileum.2004

    • Author(s)
      A Brehmer, F Schrodl, W Neuuber, I Tooyama, H Kimura
    • Journal Title

      J Chem Neuroanat 27

      Pages: 33-41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Innervation of rat iris by trigeminal and ciliary neurons expressing pChAT, a novel splice variant of choline acetyltranferase.2004

    • Author(s)
      O Yasuhara, Y Aimi, A Shibano, A Matsuo, JP Bellier, M Park, I Tooyama, H Kimura
    • Journal Title

      J Comp Neurol 472

      Pages: 232-245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fate of (D-Ala2)-deltorphin-I-like immunoreactive neurons in 6-hydroxydopamine lesioned rat brain.2004

    • Author(s)
      A Casini, A Pinna, I Tooyama, H Kimura, G Di Chiara, TG Renda
    • Journal Title

      Eur J Histochem 48

      Pages: 135-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coexpression pattern of two variants of choline acetyltransferase and neuronal nitric oxide synthase in myenteric neurons of porcine ileum.2004

    • Author(s)
      A Brehmer, F Schrodl, W Neuuber, I Tooyama, H Kimura
    • Journal Title

      Journal Chemical Neuroanatomy 27

      Pages: 33-41

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ameliorative effects of acetyl-L-carnitine and acidic fibroblast growth factor fragment analog on brain lipid hydroperoxide level, passive avoidance learning and/or immunoreactivity for choline acetyltransferase in the medial septum in senescenceaccelerated mice.2004

    • Author(s)
      K Sasaki, M Ishibashi, F Yasui, S Matsugo, I Tooyama, H Kimura, O Oomura
    • Journal Title

      International Congress Series 1260

      Pages: 123-128

    • Related Report
      2004 Annual Research Report
  • [Book] 神経内科学テキスト改訂第2版(分担執筆)2005

    • Author(s)
      遠山育夫
    • Total Pages
      10
    • Publisher
      南江堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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