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Plastic changes and long-term depression of synapses and synaptic glutamate receptors in the cerebellum by eye movement learning

Research Project

Project/Area Number 16300114
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

SHIGEMOTO Ryuichi  National Institute for Physiological Sciences, Cerebral Research, Professor, 大脳皮質機能研究系, 教授 (20221294)

Co-Investigator(Kenkyū-buntansha) NAGAO Souichi  RIKEN, Brain Science Institute, Laboratory Head, 脳科学研究総合センター, チームリーダー (40164397)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2005: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2004: ¥10,900,000 (Direct Cost: ¥10,900,000)
KeywordsGlutamate receptors / Long-term depression / cerebellum / motor learning / Horizontal optokinetic response / AMPA receptors / Purkinie cells / Synapses
Research Abstract

Short- and long-term memory is believed to be encoded by modifications in synaptic strength. Morphological substrates for the modification, however, have not been well demonstrated in vivo. We used a simple, cerebellar motor learning task, short- and long-term adaptation of horizontal optokinetic response (HOKR), to study the link between learning and underlying changes in synapses and synaptic AMPA receptors. Long-term depression of parallel fiber (PF)-Purkinje cell (PC) synapses in the flocculus is involved in this type of cerebellar motor learining. To investigate learning-dependent changes of synaptic AMPA glutamate receptors, we used SDS-digested freeze-fracture replica labeling, which can detect AMPA channels with one-to-one detection sensitivity. PF-PC synapses were identified with clusters of intramembrane particles on E-face and immunogold labeling for delta2 receptors. Almost all PF-PC synapses were labeled with immunogold particles for AMPA receptors. In short-term adaptation of HOKR induced with one hour training, we found a significant reduction of labeling for AMPA receptors at PF-PC synapses in the middle one third of the flocculus but no changes in synapse density. No such difference in AMPAR density was detected in the flocculus of untrained animals or in the paraflocculus of the trained animals. On the contrary, in long-term adaptation induced with the one hour training for five days, we detected no significant difference in AMPA receptor density but found significant reduction of synapse density in the flocculus of trained animals. These results suggest that memory traces for short-and long-term memory are differently formed in the cerebellar motor learning.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (22 results)

All 2006 2005 2004

All Journal Article (22 results)

  • [Journal Article] Localization of metabotropic GABA receptor subunits GABA1 and GABA2 relative to synaptic sites in the rat developing cerebellum.2006

    • Author(s)
      Lujan R
    • Journal Title

      Eur J Neurosci 23

      Pages: 1479-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Localisation of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum.2006

    • Author(s)
      Lujan R
    • Journal Title

      Eur J Neurosci (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Glutamate and GABA receptor signalling in the developing brain.2005

    • Author(s)
      Lujan R
    • Journal Title

      Neuroscience 130

      Pages: 567-80

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Preferential localization of the hyperpolarization-activated cyclic nucleotide-gated cation channel subunit HCN1 in basket cell terminals of the rat cerebellum.2005

    • Author(s)
      Lujan R
    • Journal Title

      Eur J Neurosci 21

      Pages: 2073-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] GABAergic basket cells expressing cholocystokinin contain vesicular glutamate transporter type 3 (VGLUT3) in their synaptic terminals in hippocampus and isocortex of the rat.2004

    • Author(s)
      Sorogyi J
    • Journal Title

      Eur J Neurosci 19

      Pages: 552-569

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Bidirestional interactions between H-channels and Na+-K+ pumps in mesencephalic trigeminal neurons.2004

    • Author(s)
      Kang Y
    • Journal Title

      J Neurosci 24

      Pages: 3694-3702

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Immunocytochemical localization of the α IA subunit of the P/Q-type calcium channel in the rat cerebellum.2004

    • Author(s)
      Kulik A
    • Journal Title

      Eur J Neurosci 19

      Pages: 2169-2178

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunolocalization of metabotropic glutamate receptor 1 alpha (mGluR l alpha) in distinct classes of interneuron in the CA1 region of the rat hippocampus.2004

    • Author(s)
      Ferraguti F
    • Journal Title

      Hippocampus 14

      Pages: 193-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Depression of GABAergic input to identified hippocampal neurons by group III metabotropic glutamate receptors in the rat.2004

    • Author(s)
      Kogo N
    • Journal Title

      Eur J Neurosci 19

      Pages: 2727-2740

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Localization of the GABAB receptor l a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat.2004

    • Author(s)
      Lujan R
    • Journal Title

      J Comp Neurol 475

      Pages: 36-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distribution of metabotropic GABA receptor subunits GABAB1 a/b and GABAB2 in the rat hippocampus during prenatal and postnatal development.2004

    • Author(s)
      Lopez-Bendito G
    • Journal Title

      Hippocampus 14

      Pages: 836-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] HCN2 and HCN1 channels govern the regularity of autonomous pacemaking and synaptic resetting in globus pallidus neurons.2004

    • Author(s)
      Chan CS
    • Journal Title

      J Neurosci 24

      Pages: 9921-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] グルタミン酸受容体の分布とシナプスでの機能。2004

    • Author(s)
      籾山明子
    • Journal Title

      蛋白質核酸酵素 49

      Pages: 287-294

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GABAergic basket cells expressing cholocystokinin contain vesicular glutamate transporter type 3 (YGLUT3) in their synaptic terminals in hippocampus and isocortex of the rat.2004

    • Author(s)
      Somogyi J
    • Journal Title

      Eur J Neurosci 19

      Pages: 552-569

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunocytochemical localization of the α1A subunit of the P/Q-type calcium channel in the rat cerebellum.2004

    • Author(s)
      Kulik A
    • Journal Title

      Eur J Neurosci 19

      Pages: 2169-2178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Immunolocalization of metabotropic glutamate receptor 1alpha (mGluRlalpha) in distinct classes of interneuron in the CA1 region of the rat hippocampus.2004

    • Author(s)
      Ferraguti F
    • Journal Title

      Hippocampus 14

      Pages: 193-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat.2004

    • Author(s)
      Lujan R
    • Journal Title

      J Comp Neurol 475

      Pages: 36-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Distribution of metabotropic GABA receptor subunits GABAB1a/b and GABAB2 in the rat hippocampus during prenatal and postnatal development.2004

    • Author(s)
      Lopez-Bendito G
    • Journal Title

      Hippocampus 14

      Pages: 836-848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Localization of glutamate receptors and their functions in syanpses2004

    • Author(s)
      Momiyama A
    • Journal Title

      Tanpaku, Kakusan, and Koso 49

      Pages: 287-294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GABAergic basket cells expressing cholocystokinin contain vesicular glutamate transporter type 3(VGLUT3) in their synaptic terminals in hippocampus and isocortex of the rat.2004

    • Author(s)
      Somogyi J
    • Journal Title

      Eur J Neurosci 19

      Pages: 552-569

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunolocalization of metabotropic glutamate receptor 1alpha(mGluR1alpha) in distinct classes of interneuron in the CA1 region of the rat hippocampus.2004

    • Author(s)
      Ferraguti F
    • Journal Title

      Hippocampus 14

      Pages: 193-215

    • Related Report
      2004 Annual Research Report
  • [Journal Article] グルタミン酸受容体の分布とシナプスでの機能2004

    • Author(s)
      籾山明子
    • Journal Title

      蛋白質核酸酵素 49

      Pages: 287-294

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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