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Voltage-dependent Na+ channel: quality control and stress response.

Research Project

Project/Area Number 16300119
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionUniversity of Miyazaki

Principal Investigator

WADA Akihiko  University of Miyazaki, Department of Pharmacology, Professor, 医学部, 教授 (30131949)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hideyuki  University of Miyazaki, Department of Pharmacology, Associate Professor, 医学部, 助教授 (40148953)
YANAGITA Toshihiko  University of Miyazaki, Department of Pharmacology, Assistant Professor, 医学部, 助手 (60295227)
YOKOO Hiroki  University of Miyazaki, Department of Pharmacology, Assistant Professor, 医学部, 助手 (30332894)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2006: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2005: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsNav1.7 Na+ channel / cell surface expression / Ca2+ overload / insulin / insulin-like growth factor / lithium / lysosphosphatidic acid / troglitazone / Na^+チャネル / 翻訳後修飾 / 糖鎖付加 / heat shock protein 90 / glycogen synthase kinase-3β / lysophosphatidic acid / lithium / Hsp90 / 細胞膜発現調節 / IGF-I / GSK-3β / β-アミロイド / インターナリゼーション / 副腎髄質クロマフィン細胞 / 電位依存性Na+チャネル / 血清除去 / β-アミロイド・ペプタイド / プリオン・ペプタイド / glycogen synthase kinase-3
Research Abstract

Among nine isoforms of voltage-dependent Na^+ channel, Na_v1.7 has received widespread attention largely as the first convincing molecular target of therapeutic drugs against pain. Abnormal up-regulation of Na_v1.7, or hyperexcitability of Na_v1.7 or sympathetic nervous system causes chronic intolerable pain (e.g. painful diabetic neuropathy and inflammatory pain). In cultured bovine adrenal chromaffin cells, we examined the mechanisms whereby various signaling molecules regulate cell surface expression of Na_v1.7, with cellular consequence of Na_v1.7 regulation.
1. Cytoplasmic : Ca^<2+> overload activated protein kinase C-α and calpain, decreasing cell surface Na_v1.7. Persistent high-degree of Ca^<2+> overload lowered Na^+ channel α-and β_1-subunit mRNA levels, while sustained (but not transient) moderate-degree of Ca^<2+> overload was sufficient to promote endocytic internalization of cell surface Na_v1.7.
2. Insulin and insulin-like growth factor-I (IGF-I), two growth factors promoti … More ng neurogenesis and neuroprotection/neuroregeneration, increased cell surface Na_v1.7 via differential mechanisms. IGF-I inhibited glycogen synthase kinase-3β (GSK-3β), increasing Na_v1.7 gene transcription and its cell surface expression; increased ^<22>Na^+ influx via up-regulate i Na_v1.7 enhanced ^<45>Ca^<2+> influx via voltage-dependent Ca^<2+> channel and exocytic secretion of catecholamines. Lithium, a therapeutics against acute neuronal injuries and chronic neurodegenerative diseases, exhibited effects similar to those of IGF-I.
3. Lysophosphatidic acid (LPA), a lipid signal causing neurogenic pain, activated LPA_1 receptor, and increased Na^+ channel α-and β_1-subunit mRNA levels, up-regulating cell surface Na_v1.7 and ^<22>Na^+ influx. Electrophysiological properties of up-regulated Na^+ channels are comparable with those of native Na_v1.7.
4. Insulin deficiency-induced diabetic neuropathy affects motor, sensory, and autonomic neurons, being attributed to dysregulated expression and localization of Na^+ channels. Troglitazone, a drug against insulin resistance, lowered cell surface number of Na_v1.7. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (45 results)

All 2007 2006 2005 2004

All Journal Article (43 results) Book (2 results)

  • [Journal Article] Voltage-dependent Na_v1.7 sodium channels : multiple roles in adrenal chromaffin cells and peripheral nervous system.2007

    • Author(s)
      Akihiko Wada et al.
    • Journal Title

      Acta Physiological (Oxford) (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Voltage-dependent Nav1.7 sodium channels : multiple roles in adrenal chromaffin cells and peripheral nervous system.2007

    • Author(s)
      Wada A et al.
    • Journal Title

      Acta Physiological (Oxford) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of voltage-dependent sodium channels in neuronal development, pain, and neurodegernation.2006

    • Author(s)
      Akihiko Wada
    • Journal Title

      Journal of Pharmacological Sciences 102・3

      Pages: 253-268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhancement of insulin-induced PI3K/Akt/GSK-3β and ERK signaling by neuronal nicotinic receptor/PKC-α/ERK pathway : up-regulation of IRS-1/-2 mRNA and protein in adrenal chromaffin cells.2006

    • Author(s)
      Takashi Sugano et al.
    • Journal Title

      Journal of Neurochemistry 98・1

      Pages: 20-33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Constitutive activity of glycogen synthase kinase-3β : positive regulation of steady-state levels of insulin receptor substrates-1 and -2 in adrenal chromaffin cells.2006

    • Author(s)
      Takayuki Nemoto et al.
    • Journal Title

      Brain Research 1110・1

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells.2006

    • Author(s)
      Hideyuki Kobayashi et al.
    • Journal Title

      Brain Research 1123・1

      Pages: 12-19

    • NAID

      120007122978

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibition by selenium compounds of catecholamine secretion due to inhibition of Ca^<2+> influx in cultured bovine adrenal chromaffin cells.2006

    • Author(s)
      Yasuhito Uezono
    • Journal Title

      Journal of Pharmacological Sciences 101・3

      Pages: 223-229

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of voltage-dependent sodium channels in neuronal development, pain, and neurodegernation.2006

    • Author(s)
      Wada A
    • Journal Title

      Journal of Pharmacological Sciences 102(3)

      Pages: 253-268

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhancement of insulin-induced PI3K/Akt/GSK-3β and ERK signaling by neuronal nicotinic receptor/PKC-a/ERK pathway : up-regulation of IRS-1/-2 mRNA and protein in adrenal chromaffin cells.2006

    • Author(s)
      Sugano T et al.
    • Journal Title

      Journal of Neurochemistry 98(1)

      Pages: 20-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Constitutive activity of glycogen synthase kinase-3β : positive regulation of steady-state levels of insulin receptor substrates-1 and-2 in adrenal chromaffin cells.2006

    • Author(s)
      Nemoto T et al.
    • Journal Title

      Brain Research 1110(1)

      Pages: 1-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells.2006

    • Author(s)
      Kobayashi H et al.
    • Journal Title

      Brain Research 1123(1)

      Pages: 12-19

    • NAID

      120007122978

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibition by selenium compounds of catecholamine secretion due to inhibition of Ca2+ influx in cultured bovine adrenal chromaffin cells.2006

    • Author(s)
      Uezono Y et al.
    • Journal Title

      Journal of Pharmacological Sciences 101(3)

      Pages: 223-229

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of voltage-dependent sodium channels in neuronal development, pain, and neurodegeneration.2006

    • Author(s)
      Akihiko Wada
    • Journal Title

      Journal of Pharmacological Sciences 102・3

      Pages: 253-268

    • NAID

      10020344805

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role of insulin signalings as a modulator of neurogenesis and neuroprotection : regulatory mechanism of Na^+ channel expression by insulin.2006

    • Author(s)
      Toshihiko Yanagita et al.
    • Journal Title

      Journal of Pharmacological Sciences 100(Suppl.I)

      Pages: 52-52

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lithium-induced inhibition of Na^+ channel activity and up-regulation of cell surface Na^+ channel expression.2006

    • Author(s)
      Toshihiko Yanagita et al.
    • Journal Title

      Journal of Pharmacological Sciences 100(Suppl.I)

      Pages: 101-101

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lysophosphatidic acid-induced up-regulation of cell surface Na_V1.7 Na^+ channels in cultured bovine adrenal chromaffin cells.2006

    • Author(s)
      Toyoaki Maruta et al.
    • Journal Title

      Journal of Pharmacological Sciences 100(Suppl.I)

      Pages: 85-85

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role or insulin signalings as a modulator of neurogenesis and neuroprotection : regulatory mechanism of Na^+ channel expression by insulin.2006

    • Author(s)
      Toshihiko Yanagita et al.
    • Journal Title

      Journal of Pharmacological Sciences 100・Suppl.1

      Pages: 52-52

    • Related Report
      2005 Annual Research Report
  • [Journal Article] New twist on neuronal insulin receptor signaling in health, disease, and therapeutics.2005

    • Author(s)
      Akihiko Wada et al.
    • Journal Title

      Journal of Pharmacological Sciences 99・2

      Pages: 128-143

    • NAID

      10025730645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Lithium : potential therapeutics against acute brain injuries and chronic neurodegenerative diseases.2005

    • Author(s)
      Akihiko Wada et al.
    • Journal Title

      Journal of Pharmacological Sciences 99・4

      Pages: 307-321

    • NAID

      10025731486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] インスリン受容体シグナル伝達分子の発現調節機構.2005

    • Author(s)
      横尾宏毅 他
    • Journal Title

      日本薬理学雑誌 125・3

      Pages: 141-146

    • NAID

      10015568253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Insulin induces internalization of the 5HT_<2A> receptor expressed in HEK293 cells.2005

    • Author(s)
      Masamichi Ohkura et al.
    • Journal Title

      European Journal of Pharmacology 518・1

      Pages: 18-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relaxant effect of adrenomedullin on bovine isolated iris sphincter muscle under resting conditions.2005

    • Author(s)
      Yoshikazu Uchikawa et al.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 32・8

      Pages: 675-680

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Beyond vasodilation : the antioxidant effect of adrenomedullin in Dahl salt-sensitive rat aorta.2005

    • Author(s)
      Yuan-Ning Cao et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 332・3

      Pages: 866-872

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New twist on neuronal insulin receptor signaling in health, disease, and therapeutics.2005

    • Author(s)
      Wada A et al.
    • Journal Title

      Journal of Pharmacological Sciences 99(2)

      Pages: 128-143

    • NAID

      10025730645

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lithium : potential therapeutics against acute brain injuries and chronic neurodegenerative diseases.2005

    • Author(s)
      Wada A et al.
    • Journal Title

      Journal of Pharmacological Sciences 99(4)

      Pages: 307-321

    • NAID

      10025731486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of insulin receptor and its signaling molecules : regulatory mechanisms in neuronal cells.2005

    • Author(s)
      Yokoo H et al.
    • Journal Title

      Nippon Yakurigaku Zasshi 125(3)

      Pages: 141-146

    • NAID

      10015568253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Insulin induces internalization of the 5HT2A receptor expressed in HEK293 cells.2005

    • Author(s)
      Ohkura M et al.
    • Journal Title

      European Journal of Pharmacology 518(1)

      Pages: 18-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relaxant effect of adrenomedullin on bovine isolated iris sphincter muscle under resting conditions.2005

    • Author(s)
      Uchikawa Y et al.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 32(8)

      Pages: 675-680

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Beyond vasodilation : the antioxidant effect of adrenomedullin in Dahl salt-sensitive rat aorta.2005

    • Author(s)
      Cao YN et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 332(3)

      Pages: 866-872

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lithium : Potential therapeutics against acute brain injuries And chronic neurodegenerative diseases.2005

    • Author(s)
      Akihiko Wada et al.
    • Journal Title

      Journal of Pharmacological Sciences 99・4

      Pages: 307-321

    • NAID

      10025731486

    • Related Report
      2005 Annual Research Report
  • [Journal Article] インスリン受容体シグナル伝達分子の発現調節機構2005

    • Author(s)
      横尾 宏毅 他
    • Journal Title

      日本薬理学雑誌 125・3

      Pages: 141-146

    • NAID

      10015568253

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of cell surface expression of voltage-dependent Na_v1.7 sodium channels : mRNA stability and posttranscriptional control in adrenal chromaffin cells.2004

    • Author(s)
      Akihiko Wada et al.
    • Journal Title

      Frontiers in Bioscience 9

      Pages: 1954-1966

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] アドレノメデュリン 薬理作用と生理作用 副腎髄質交感神経に対する作用.2004

    • Author(s)
      和田 明彦
    • Journal Title

      日本臨床 62・Suppl9

      Pages: 252-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in PC12 cells after exposure to nerve growth factor.2004

    • Author(s)
      Hideyuki Kobayashi et al.
    • Journal Title

      Neuroscience 125・4

      Pages: 973-980

    • NAID

      120007122980

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Molecular mechanisms and drug development in aquaporin water channel diseases : aquaporins in the brain.2004

    • Author(s)
      Hideyuki Kobayashi et al.
    • Journal Title

      Journal of Pharmacological Sciences 96・3

      Pages: 264-270

    • NAID

      10014167168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of cell surface expression of voltage-dependent Nav1.7 sodium channels : mRNA stability and posttranscriptional control in adrenal chromaffin cells.2004

    • Author(s)
      Wada A et al.
    • Journal Title

      Frontiers in Bioscience 9

      Pages: 1954-1966

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The adrenomedullin family and sympathoadrenal system.2004

    • Author(s)
      Wada A
    • Journal Title

      Nippon Rinsho 62(Suppl 9)

      Pages: 252-255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in PC12 cells after exposure to nerve growth factor.2004

    • Author(s)
      Kobayashi H et al.
    • Journal Title

      Neuroscience 125(4)

      Pages: 973-980

    • NAID

      120007122980

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular mechanisms and drug development in aquaporin water channel diseases : aquaporins in the brain.2004

    • Author(s)
      Kobayashi H et al.
    • Journal Title

      Journal of Pharmacological Sciences 96(3)

      Pages: 264-270

    • NAID

      10014167168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of cell surface expression of voltage-dependent Na_v1.7 sodium Channels : mRNA stability and posttranscriptional control in adrenal chromaffin cells.2004

    • Author(s)
      Akihiko Wada et al.
    • Journal Title

      Frontiers in Bioscience 9

      Pages: 1954-1966

    • Related Report
      2004 Annual Research Report
  • [Journal Article] アドレノメデュリン 薬理作用と生理作用 副賢髄質交感神経系に対する作用2004

    • Author(s)
      和田 明彦
    • Journal Title

      臨床分子内分泌学(1)(日本臨床) 62・Suppl 9

      Pages: 252-255

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular mechanisms and drug development in aquaporin water channel diseases : aquaporins in the brain.2004

    • Author(s)
      Hideyuki Kobayashi et al.
    • Journal Title

      Journal of Pharmacological Sciences 96

      Pages: 264-270

    • NAID

      10014167168

    • Related Report
      2004 Annual Research Report
  • [Journal Article] インスリン受容体シグナル伝達分子の発現調節機構2004

    • Author(s)
      横尾 宏毅 他
    • Journal Title

      日本薬理学雑誌 125

      Pages: 141-146

    • NAID

      10015568253

    • Related Report
      2004 Annual Research Report
  • [Book] 心臓ナビゲーター2005

    • Author(s)
      和田 明彦
    • Publisher
      メディカルトレビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 心臓ナビゲーター2004

    • Author(s)
      和田 明彦
    • Total Pages
      4
    • Publisher
      メディカルレビュー社
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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