| Project/Area Number |
16300130
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Gunma University |
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Principal Investigator |
KUROMI Hiroshi Gunma University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (30009633)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Kohei Gunma University, Graduate School of Medicine, Assistant Professor, 大学院・医学系研究科, 助手 (40332556)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2005: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 2004: ¥8,400,000 (Direct Cost: ¥8,400,000)
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| Keywords | synaptic vesicle / recycling / endocytosis / drosophila / exocytosis / channel / active-zone / La3+ / ノンアクチブゾーン / シナプス伝達 / 高頻度刺激 / Ca2+チャネル / PLTXII / タンパクリン酸化 |
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| Research Abstract |
We wish to clarify mechanisms of endocytosis of synaptic vesicles and roles of endocytosis in synaptic transmission at Drosophila Neuromuscular junction.
1. It has been believed that exocytosis and endocytosis of synaptic vesicles are controlled by the same Ca2+ influx, because endocytosis always follows exocytosis and both process require external Ca2+. We found that La3+ and fulunarizine, Ca2+ channel blocker, depressed endocytosis without affecting exocytosis, while PLTXII, Ca2+ channel blocker from spider toxin, depressed predominantly exocytosis. These results suggest that Ca2+ influx through distinct routes controls exocytosis and endocytosis.
2. Using Ca2+ channel blockers, we revealed two distinct endocytosis; active-zone endocytosis and non-active-zone endocytosis. Active-zone endocytosis is clathrin-independent and operate early phase of high activity, whereas non-active-zone endocytosis is clathrin-dependent and works in late phase of high activity. These results suggest that synaptic transmission at high activity is maintained coordinately by two types of endocytosis.
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